FastQCFastQC Report
Wed 25 May 2016
SRR1780626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45678
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAA150.00221175569.9956248
GGGAGAA150.00221175569.9956214
AGAACTC150.00221175569.9956217
ATTGCTT150.00221175569.9956220
TCTGTGC252.3674448E-455.99649424
TTGGTCA252.3674448E-455.99649421
GGGTATA200.00679211252.7277221
TCGTTCG200.00691127552.4967123
CTACTGG200.00691127552.496719
GGGTACA200.00691127552.4967162
CGTTCGG200.00691127552.4967124
TGGCCAA200.00691127552.4967110
GGCCAAC200.00691127552.4967111
GTGCTAG200.00691127552.4967127
ACCACGT200.00691127552.4967136
TTCGGTA200.00691127552.4967126
CGGTAAG200.00691127552.4967128
AAGGACT200.00691127552.496714
GTAACCA200.00691127552.4967133
GACTATG200.00691127552.496717
Produced by FastQC (version 0.11.5)