##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780626_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45678 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.969897981522834 34.0 31.0 34.0 12.0 34.0 2 31.06442926572967 34.0 32.0 34.0 23.0 34.0 3 31.18967555497176 34.0 33.0 34.0 23.0 34.0 4 31.5242786461754 34.0 34.0 34.0 23.0 34.0 5 31.465059766189412 34.0 34.0 34.0 23.0 34.0 6 34.393909540697926 38.0 36.0 38.0 24.0 38.0 7 33.35233591663383 37.0 34.0 38.0 21.0 38.0 8 32.471014492753625 37.0 31.0 38.0 11.0 38.0 9 33.252835062831124 37.0 34.0 38.0 21.0 38.0 10-11 33.630653706379434 37.0 34.0 38.0 21.5 38.0 12-13 33.987718376461316 38.0 34.0 38.0 22.0 38.0 14-15 33.56635579491221 37.0 34.0 38.0 21.0 38.0 16-17 33.466833048732425 37.0 34.0 38.0 21.0 38.0 18-19 33.460637506020404 37.0 34.0 38.0 21.0 38.0 20-21 33.53480887954814 37.0 34.0 38.0 21.0 38.0 22-23 33.463855685450326 37.0 34.0 38.0 21.0 38.0 24-25 33.88681641052585 38.0 34.0 38.0 21.5 38.0 26-27 33.934169622137574 38.0 34.0 38.0 21.5 38.0 28-29 34.03434913962958 38.0 34.0 38.0 22.5 38.0 30-31 33.92833530364727 38.0 34.0 38.0 21.5 38.0 32-33 33.66263846928499 37.5 34.0 38.0 21.0 38.0 34-35 33.558507377731075 37.0 34.0 38.0 21.0 38.0 36-37 33.538377337011255 37.0 34.0 38.0 21.0 38.0 38-39 34.0560992162529 38.0 34.0 38.0 22.5 38.0 40-41 33.56106878584877 37.5 34.0 38.0 21.0 38.0 42-43 33.8633477823022 38.0 34.0 38.0 21.5 38.0 44-45 32.99192171285958 37.0 33.5 38.0 16.0 38.0 46-47 33.668286702570164 37.0 34.0 38.0 21.0 38.0 48-49 33.437234554927976 37.0 34.0 38.0 21.0 38.0 50-51 33.38912605630719 37.0 34.0 38.0 21.0 38.0 52-53 33.461075353561895 37.0 34.0 38.0 21.0 38.0 54-55 33.17773326327773 37.0 33.5 38.0 21.0 38.0 56-57 33.24856604930163 37.0 33.5 38.0 20.0 38.0 58-59 33.101372652042556 37.0 33.0 38.0 16.0 38.0 60-61 33.16507946932878 37.0 33.0 38.0 20.0 38.0 62-63 33.17983493147686 37.0 33.0 38.0 20.0 38.0 64-65 33.21495030430404 37.0 33.0 38.0 20.5 38.0 66-67 33.1331165988003 37.0 33.0 38.0 20.0 38.0 68-69 32.759161959805596 37.0 31.5 38.0 15.5 38.0 70-71 32.59547265642104 37.0 31.5 38.0 14.0 38.0 72-73 32.70343491396296 37.0 31.5 38.0 18.5 38.0 74-75 32.26310258767897 37.0 31.0 38.0 15.0 38.0 76 31.380577083059677 36.0 27.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.9456657524769057 1101 2 -1.5283992957762642 1101 3 -0.7656394884627495 1101 4 -2.350189937242007 1101 5 -1.181412963985803 1101 6 -1.1831801812564748 1101 7 -1.7425328672550329 1101 8 -0.6285438737011866 1101 9 -0.6692324333673056 1101 10-11 -0.3558243338870568 1101 12-13 -1.551835496684376 1101 14-15 -0.10033459966108182 1101 16-17 -1.2611723375352852 1101 18-19 0.9427917147438833 1101 20-21 -0.4298037452643868 1101 22-23 -0.5609333943586705 1101 24-25 -1.166470741108661 1101 26-27 -0.3369700417502983 1101 28-29 -0.5206065149764214 1101 30-31 -0.9025665850372064 1101 32-33 0.501935276195077 1101 34-35 -0.7806781600427541 1101 36-37 0.49507237611815214 1101 38-39 -0.7264900807813532 1101 40-41 0.5099376261560167 1101 42-43 0.006287724887762636 1101 44-45 -0.03142732301699169 1101 46-47 0.9758224918045997 1101 48-49 -0.6853868484407428 1101 50-51 -0.9260538968952332 1101 52-53 -0.2937493357063019 1101 54-55 -1.165816305329443 1101 56-57 0.21872565080700923 1101 58-59 -0.12184768120255285 1101 60-61 -0.4034652168957038 1101 62-63 -0.4989122347231003 1101 64-65 -0.07291594183179484 1101 66-67 -0.1317402368687226 1101 68-69 0.36712656669664767 1101 70-71 0.34861410208019095 1101 72-73 -0.09879348430756352 1101 74-75 -0.7909599307557045 1101 76 -0.32108963530829016 1102 1 0.17118584253002922 1102 2 -0.9284513068719633 1102 3 -1.051960570293538 1102 4 -0.23420519383007843 1102 5 -0.017751382848491204 1102 6 0.14899734995018576 1102 7 0.604641196545245 1102 8 2.0547085534832803 1102 9 0.9318424626104971 1102 10-11 -0.8835635849272734 1102 12-13 -4.164569546615024 1102 14-15 -0.7864909796888213 1102 16-17 -2.3515416163147513 1102 18-19 -0.18238165557512076 1102 20-21 -0.31381900185245115 1102 22-23 0.17974795099500085 1102 24-25 0.2105230175598578 1102 26-27 -5.287342787936147 1102 28-29 -0.7766744761414657 1102 30-31 0.23578292952590374 1102 32-33 -6.600439897175242 1102 34-35 -0.009830379182837135 1102 36-37 0.6184687006673926 1102 38-39 0.23840242962086222 1102 40-41 0.48874310465809856 1102 42-43 0.32407551961730263 1102 44-45 -5.20826504839841 1102 46-47 -0.3237614194297933 1102 48-49 0.7034827770793655 1102 50-51 -5.543122204800923 1102 52-53 -1.5355575881334858 1102 54-55 0.6140467321185383 1102 56-57 -0.2510212285272502 1102 58-59 0.7226391426532004 1102 60-61 0.3464047553650502 1102 62-63 -4.060762096026846 1102 64-65 -0.5755164966168138 1102 66-67 0.06252120557233098 1102 68-69 -8.699707691278391 1102 70-71 -1.2517673126216167 1102 72-73 -0.6058583248068672 1102 74-75 -6.832482122156538 1102 76 -1.0307810361404677 1103 1 0.3178891392333263 1103 2 -0.4854568013774596 1103 3 0.4404844846515168 1103 4 0.6342013995765114 1103 5 1.0723585072613986 1103 6 -0.3707828698300375 1103 7 1.085547789951839 1103 8 1.4450931688678992 1103 9 0.19200729777533354 1103 10-11 0.3609419095782158 1103 12-13 -0.804748118043598 1103 14-15 -0.10455416650200533 1103 16-17 0.14371937269623913 1103 18-19 -0.3384256116190798 1103 20-21 -0.28772010075355325 1103 22-23 -0.8427108402137904 1103 24-25 0.354066973603814 1103 26-27 -0.8663949857383457 1103 28-29 0.74832552385854 1103 30-31 0.42809062183359714 1103 32-33 -4.516511325746674 1103 34-35 -0.1306408187432737 1103 36-37 0.037081338030027666 1103 38-39 0.7135397922582243 1103 40-41 0.6474656321306185 1103 42-43 0.5930315635733479 1103 44-45 -2.9269463670797293 1103 46-47 0.4592056135372431 1103 48-49 0.0312300298266166 1103 50-51 -0.1286304465591641 1103 52-53 -0.03411528044117773 1103 54-55 -0.11940107007926315 1103 56-57 1.2930721780661614 1103 58-59 1.249287494301548 1103 60-61 1.1461987114090064 1103 62-63 0.42199889298414206 1103 64-65 1.1511318550315366 1103 66-67 -0.04503373948261924 1103 68-69 -2.9094604385311342 1103 70-71 -0.07621786207216275 1103 72-73 0.9752543125557764 1103 74-75 -0.6136634408378576 1103 76 0.15232335946392794 1104 1 -1.3505911609542913 1104 2 1.344124186740114 1104 3 1.3867008698922945 1104 4 1.052525352046807 1104 5 0.817324110763586 1104 6 0.8963841095320646 1104 7 1.594115644861155 1104 8 1.9500337567353156 1104 9 1.1793744022156076 1104 10-11 0.8098162060134939 1104 12-13 0.5127315567531525 1104 14-15 1.4055458960370828 1104 16-17 0.9231752826399529 1104 18-19 2.1303361144597233 1104 20-21 2.3997408123171198 1104 22-23 1.771610610693024 1104 24-25 1.9572564670372046 1104 26-27 2.271128466419249 1104 28-29 1.2873499141024354 1104 30-31 -0.05893251762856977 1104 32-33 1.433895178318373 1104 34-35 1.3784898879484047 1104 36-37 1.4859556344653058 1104 38-39 1.6089744389123766 1104 40-41 1.9128502475152374 1104 42-43 2.2699233709529594 1104 44-45 -1.9068713201754122 1104 46-47 2.0850655259825146 1104 48-49 2.4126871905520773 1104 50-51 2.705171929926145 1104 52-53 1.4301436313787121 1104 54-55 0.9768153151615167 1104 56-57 2.000357981693419 1104 58-59 2.272145530574221 1104 60-61 2.338819099151344 1104 62-63 2.257145859838424 1104 64-65 2.0288679400846945 1104 66-67 1.334515979091492 1104 68-69 -2.025564253415439 1104 70-71 1.0831630009672324 1104 72-73 2.059900966714622 1104 74-75 -0.6139135971855758 1104 76 1.4163008453926373 1105 1 0.5791945005386872 1105 2 0.6876201216994637 1105 3 0.19398098814802012 1105 4 0.509842425217542 1105 5 0.18354731845020922 1105 6 0.08189778285061777 1105 7 1.7975524519564985 1105 8 1.3615267353014602 1105 9 0.949699605467643 1105 10-11 0.5439255925619051 1105 12-13 -0.9206572089526901 1105 14-15 0.6335577216098756 1105 16-17 1.034686738663602 1105 18-19 1.0530079548144897 1105 20-21 0.5211377081042556 1105 22-23 0.7145152670123167 1105 24-25 0.958087952624787 1105 26-27 1.26041154106818 1105 28-29 -0.6024320518990436 1105 30-31 0.747146565889544 1105 32-33 0.7077310984957492 1105 34-35 1.3588113957089405 1105 36-37 0.7844589604076546 1105 38-39 0.42373792745636507 1105 40-41 0.9658805505455348 1105 42-43 0.36249543303721765 1105 44-45 1.517925427792072 1105 46-47 1.4787277580593852 1105 48-49 1.2245866731832606 1105 50-51 0.4226516047228941 1105 52-53 -0.20695910328500133 1105 54-55 0.02273179705360917 1105 56-57 0.8742493342433093 1105 58-59 0.38227117728524007 1105 60-61 0.5380984783087754 1105 62-63 0.6417541377393903 1105 64-65 0.26593371983340575 1105 66-67 0.7554324609835845 1105 68-69 1.5354113563406528 1105 70-71 2.197908012053716 1105 72-73 0.8953592076606611 1105 74-75 -0.3468219489963644 1105 76 -0.24371393657336782 1106 1 -0.37455350584089686 1106 2 0.31481310415560415 1106 3 -1.2761147056318833 1106 4 -0.04717511864210877 1106 5 0.6125744316878468 1106 6 0.9184209088474233 1106 7 0.7006624998033928 1106 8 0.8946208341850337 1106 9 1.8663662721343073 1106 10-11 -0.05336308367414233 1106 12-13 0.8127957416055267 1106 14-15 0.6897778173036642 1106 16-17 0.5923023686476512 1106 18-19 0.10145293089104968 1106 20-21 0.13198300316008016 1106 22-23 0.026317499867175798 1106 24-25 1.2062936942515918 1106 26-27 0.5598533273520658 1106 28-29 -0.9104464059660273 1106 30-31 0.10420398215747895 1106 32-33 1.2922606040778888 1106 34-35 0.9977667386116522 1106 36-37 0.22365353776012853 1106 38-39 0.9857395223526879 1106 40-41 1.1365344580415524 1106 42-43 1.6890504569606648 1106 44-45 1.7591534979675032 1106 46-47 1.1117022397180776 1106 48-49 0.5208386668036766 1106 50-51 0.6156340608632362 1106 52-53 0.27310469256826764 1106 54-55 0.9806664063040103 1106 56-57 1.277957468214602 1106 58-59 -0.008877148073615615 1106 60-61 1.6870219232848527 1106 62-63 1.1890029415671393 1106 64-65 1.723668967042336 1106 66-67 2.206788122865561 1106 68-69 1.9652359177441667 1106 70-71 1.2003003565513168 1106 72-73 2.3118265122858617 1106 74-75 -1.1819495407029024 1106 76 -0.32824344215550383 1107 1 -0.13443029705557663 1107 2 0.06739111406587739 1107 3 0.9078949367947899 1107 4 0.22040222165519907 1107 5 0.11311012205881354 1107 6 0.597743260528155 1107 7 0.6694585356946448 1107 8 1.7274184771474026 1107 9 -0.6209110815552563 1107 10-11 0.3825214750509147 1107 12-13 0.8429618056205257 1107 14-15 -0.06665046714792311 1107 16-17 1.1231380456263622 1107 18-19 1.3855027500955472 1107 20-21 1.1026204747015314 1107 22-23 -0.09250532054262095 1107 24-25 0.2137263981487294 1107 26-27 0.83489685219471 1107 28-29 -0.9799187509506311 1107 30-31 0.40181346387704764 1107 32-33 0.9552443994441617 1107 34-35 0.5949610602937128 1107 36-37 0.14263036915158267 1107 38-39 0.6381434328923987 1107 40-41 -0.014630668515295042 1107 42-43 0.7383339710894958 1107 44-45 0.7094012547640816 1107 46-47 0.9537740222277833 1107 48-49 0.725974598235311 1107 50-51 0.7998780678272155 1107 52-53 1.0524672210268164 1107 54-55 0.8186200690869185 1107 56-57 0.8759553254531269 1107 58-59 1.5701037009956238 1107 60-61 0.650896303892857 1107 62-63 0.4811885582806781 1107 64-65 0.7853068403515664 1107 66-67 1.3468041935766877 1107 68-69 1.041772679495871 1107 70-71 0.16303639512102563 1107 72-73 1.1983605955857186 1107 74-75 0.7211574634486908 1107 76 -0.4021687133487539 1108 1 -1.0855633138254284 1108 2 -0.22773420900919916 1108 3 -0.02165967040602368 1108 4 0.17454058794719884 1108 5 -0.10886499364714197 1108 6 0.25417170315603244 1108 7 0.6723436712359074 1108 8 1.1304959550580804 1108 9 -0.6388830716977125 1108 10-11 0.03432167941468123 1108 12-13 -0.3012048109569818 1108 14-15 0.17579107880386857 1108 16-17 -1.686292741174146 1108 18-19 -0.4093220380273124 1108 20-21 -0.4641283381854109 1108 22-23 -0.90437044045607 1108 24-25 -0.8100759700666913 1108 26-27 -1.0522823267438 1108 28-29 0.1475381222837342 1108 30-31 0.34011363818731866 1108 32-33 -0.48029085330572485 1108 34-35 -0.6358497830073588 1108 36-37 0.8876264621972041 1108 38-39 0.864175770453258 1108 40-41 0.33804709790894094 1108 42-43 0.7418201765351924 1108 44-45 0.4385291023327511 1108 46-47 0.5311317189279094 1108 48-49 0.26324093230996226 1108 50-51 0.28944950498535604 1108 52-53 -0.09338239128308601 1108 54-55 0.34404532604429505 1108 56-57 0.831866847963191 1108 58-59 -0.2749705311375763 1108 60-61 0.5056778149825973 1108 62-63 0.16009701533423737 1108 64-65 0.41703130990737236 1108 66-67 -0.0016660835165325238 1108 68-69 1.3083772356057537 1108 70-71 1.592861483778833 1108 72-73 0.49745298003003313 1108 74-75 1.2793710836927303 1108 76 1.0807909548606567 1109 1 -0.9063273849832001 1109 2 -1.706824322744982 1109 3 -0.17319072902369825 1109 4 -0.8920094266343135 1109 5 -0.255846620943732 1109 6 0.0468809478337775 1109 7 -1.199922295518249 1109 8 -0.17029144651672112 1109 9 0.5071070128750534 1109 10-11 0.44712424576055554 1109 12-13 0.08969632640084768 1109 14-15 0.37934896740112833 1109 16-17 -0.45984188086501376 1109 18-19 -0.05195837515183399 1109 20-21 0.4802286171951664 1109 22-23 0.674952977450026 1109 24-25 -0.08240026286342328 1109 26-27 -0.7712382905816568 1109 28-29 -0.3368596613266561 1109 30-31 0.17837552211849328 1109 32-33 0.7390442298088828 1109 34-35 -0.10642429452674662 1109 36-37 0.4565970075457031 1109 38-39 0.11809819681662503 1109 40-41 -0.6834460487810574 1109 42-43 -0.20198268144089582 1109 44-45 -0.5357782759116319 1109 46-47 -1.0375180331864087 1109 48-49 -0.6572315086349221 1109 50-51 0.48746641953770364 1109 52-53 0.05634056001466092 1109 54-55 0.3058126051344132 1109 56-57 -0.01960588461190582 1109 58-59 0.1169513119653729 1109 60-61 0.5356574008676915 1109 62-63 -1.157488286503039 1109 64-65 -0.04231543841575558 1109 66-67 0.25240215795328425 1109 68-69 0.553929874859179 1109 70-71 0.19201575616145306 1109 72-73 -0.2533781660767076 1109 74-75 1.4182453910709683 1109 76 0.6175655247060909 1110 1 0.6513445726887603 1110 2 0.2868264709058117 1110 3 -0.0668415226744905 1110 4 0.5376202029953205 1110 5 0.9584390933419868 1110 6 -0.43036772941489687 1110 7 0.9746808790849286 1110 8 1.8820895058642293 1110 9 -1.9669670611990249 1110 10-11 -0.9192778706415581 1110 12-13 -0.2166528799483558 1110 14-15 -0.6312330431808846 1110 16-17 0.23724806622493588 1110 18-19 0.20650723331376497 1110 20-21 -0.280088843122293 1110 22-23 -0.20864490614786035 1110 24-25 0.1718325413693762 1110 26-27 -2.143989613332973 1110 28-29 -0.3421506666176555 1110 30-31 -0.5380265942836218 1110 32-33 0.7676156583803149 1110 34-35 0.8120942239917639 1110 36-37 -0.1399638390151452 1110 38-39 -0.004653125934694913 1110 40-41 -0.2855624508974586 1110 42-43 -0.36441654387476063 1110 44-45 0.7837984436650842 1110 46-47 -0.26026935593773004 1110 48-49 0.06657801517459916 1110 50-51 -0.11703093495965078 1110 52-53 -0.03704568337158065 1110 54-55 -0.6116477123259045 1110 56-57 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-0.18507197251287977 2116 52-53 0.5073794037364365 2116 54-55 -0.4622090750823915 2116 56-57 -0.007772099607386451 2116 58-59 -0.37419593284041497 2116 60-61 -0.6367906569462178 2116 62-63 0.6555198435782685 2116 64-65 0.37846143601966276 2116 66-67 -0.283370199770296 2116 68-69 -0.3101170989438984 2116 70-71 -2.8599264299270715 2116 72-73 0.45827495045445943 2116 74-75 -1.268385141901021 2116 76 -0.9414227318431401 2117 1 -1.2015820146128284 2117 2 -1.4920673783005363 2117 3 0.32750371542074674 2117 4 -0.8859909081157937 2117 5 -1.8626174542770642 2117 6 -2.23604729290696 2117 7 -4.09401951774047 2117 8 -3.3593539465167197 2117 9 -0.261237894532357 2117 10-11 -1.234010013498704 2117 12-13 -0.4391231180435966 2117 14-15 -1.5766695511173907 2117 16-17 -1.392278223457609 2117 18-19 -1.1952164770036902 2117 20-21 -1.2429484661381665 2117 22-23 -0.5071940132907145 2117 24-25 -1.1789859110115728 2117 26-27 0.2020545334924222 2117 28-29 0.055356773858534325 2117 30-31 -0.19781082047409626 2117 32-33 0.7170824242533271 2117 34-35 -0.7369509148971218 2117 36-37 -2.237558085046892 2117 38-39 -0.4719770346648531 2117 40-41 -2.5988685024847626 2117 42-43 -2.5246968018112668 2117 44-45 -0.5068141555412637 2117 46-47 -1.232801598001224 2117 48-49 -1.3752002586349192 2117 50-51 -0.9080775619437773 2117 52-53 -0.6449325881334858 2117 54-55 -3.176131839310031 2117 56-57 -2.696891764241535 2117 58-59 -2.087070678775369 2117 60-61 -0.4727916732063804 2117 62-63 -1.7694674531697032 2117 64-65 -1.4946013180453832 2117 66-67 -2.7650457587133843 2117 68-69 -1.1885469769926758 2117 70-71 0.13718358023552923 2117 72-73 0.7295211394788481 2117 74-75 -1.7091562292993956 2117 76 -2.82207567899761 2118 1 -0.21595701461282957 2118 2 -0.12637987830053632 2118 3 0.4312537154207483 2118 4 -0.3334909081157953 2118 5 -0.04517995427706367 2118 6 0.2821402070930432 2118 7 0.3058554822595312 2118 8 -3.9702914465167183 2118 9 -1.0443003945323568 2118 10-11 -1.200135013498702 2118 12-13 0.0692518819564043 2118 14-15 -1.0071695511173857 2118 16-17 -1.4084344734576035 2118 18-19 -1.9238102270036883 2118 20-21 -3.8691047161381675 2118 22-23 -1.0857877632907176 2118 24-25 -0.8705484110115762 2118 26-27 0.28283578349242333 2118 28-29 -0.9596744761414655 2118 30-31 -2.278217070474099 2118 32-33 -0.7805113257466729 2118 34-35 0.18320533510288328 2118 36-37 -1.247995585046894 2118 38-39 -0.7993832846648488 2118 40-41 -1.8991497524847638 2118 42-43 -0.9403530518112646 2118 44-45 -1.1774079055412656 2118 46-47 -1.2883328480012253 2118 48-49 -1.0132315086349166 2118 50-51 -0.6900150619437753 2118 52-53 -1.7358075881334862 2118 54-55 -0.52263183931003 2118 56-57 0.9187644857584658 2118 58-59 -0.4357894287753723 2118 60-61 -3.446416673206379 2118 62-63 -0.26815495316970583 2118 64-65 -1.5319450680453812 2118 66-67 -3.178264508713383 2118 68-69 0.1340780230073264 2118 70-71 -1.2859101697644704 2118 72-73 -1.9938226105211498 2118 74-75 -1.1281249792993968 2118 76 -0.8061381789976103 2119 1 -1.1925941827544229 2119 2 0.15000950223043574 2119 3 -0.019011771304917602 2119 4 0.07252679099925174 2119 5 -0.34139234365759563 2119 6 0.9220694106328651 2119 7 -2.103560446944009 2119 8 -0.43754808368485953 2119 9 -0.11445968656775563 2119 10-11 -0.3740819161535711 2119 12-13 -0.215810064946254 2119 14-15 -1.825010259081992 2119 16-17 -0.36216898673194464 2119 18-19 -1.9108898730213895 2119 20-21 -1.25044984888153 2119 22-23 0.6996989623730059 2119 24-25 -1.0438227472947617 2119 26-27 0.16655259765170882 2119 28-29 0.2952281787257931 2119 30-31 -0.5053675129519704 2119 32-33 -0.849254688578533 2119 34-35 -0.9838743109148211 2119 36-37 -1.0386504523035285 2119 38-39 -0.5129408067887482 2119 40-41 -0.9676807259360913 2119 42-43 -1.0758928748201129 2119 44-45 -1.5698326843023267 2119 46-47 -1.1992355028684827 2119 48-49 -0.9926297387234158 2119 50-51 0.24049821239250235 2119 52-53 -1.2626040483104788 2119 54-55 -1.3315521932923318 2119 56-57 0.3454547512451924 2119 58-59 0.7939096862688828 2119 60-61 0.5491939462626476 2119 62-63 -1.2445089354705843 2119 64-65 0.08203723283956776 2119 66-67 0.30785938509193045 2119 68-69 -1.213851180532501 2119 70-71 -1.3843526476405756 2119 72-73 -2.9836633184857533 2119 74-75 -0.47778869611355645 2119 76 0.7793485466661103 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 73.0 14 165.0 15 340.0 16 450.0 17 560.0 18 573.0 19 596.0 20 537.0 21 484.0 22 437.0 23 403.0 24 443.0 25 531.0 26 605.0 27 686.0 28 865.0 29 1003.0 30 1260.0 31 1564.0 32 1939.0 33 2517.0 34 3502.0 35 5323.0 36 9184.0 37 11622.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.549143272983876 25.796634838026772 11.277482523650958 28.376739365338388 2 16.182845133324577 23.26283987915408 36.371995271246554 24.182319716274794 3 16.180655895617146 26.222689259599807 30.66684180568326 26.929813039099788 4 12.200621743508911 19.24996716143439 37.873812338543715 30.675598756512983 5 11.734314111826261 36.34134594334253 37.11414685406542 14.810193090765795 6 25.98625158719734 40.22724287403126 17.645255921887998 16.141249616883403 7 24.042208502999255 30.231183501904635 23.254082928324358 22.47252506677175 8 21.204956434169624 35.019046368054646 21.187442532510183 22.588554665265555 9 22.603879329217566 16.655720478129517 22.314899951836768 38.42550024081615 10-11 23.938219711896316 26.107754279959718 27.12465519506108 22.829370813082882 12-13 23.051578440387058 23.673321949297254 26.456937694294847 26.818161916020845 14-15 20.786812032050438 25.95779149700074 24.112264109637024 29.143132361311793 16-17 21.505976618941286 28.19409781514077 25.765138578746882 24.534786987171067 18-19 21.317702176102284 27.20346775252857 26.370462804851353 25.1083672665178 20-21 20.755068085292702 27.905118437759974 26.254433206357547 25.08538027058978 22-23 22.04015061955427 27.122465957353647 26.558737247690356 24.278646175401725 24-25 21.996365865405664 28.009107228862913 25.582337230176456 24.41218967555497 26-27 20.366478392223826 28.48636104908271 27.094005867157055 24.053154691536406 28-29 21.276106659661107 27.14216909672052 28.35610140549061 23.225622838127762 30-31 21.23888961863479 27.44866237576076 26.98782783834669 24.32462016725776 32-33 20.19462323219055 28.190813958579625 27.01409869083585 24.600464118393976 34-35 21.43701563115723 27.71356013835982 27.199089277113707 23.65033495336924 36-37 21.75445509873462 27.347957441218966 26.75576864135908 24.141818818687334 38-39 21.150225491483866 27.62380139235518 27.901834581198827 23.324138534962128 40-41 20.949910241253995 26.211743071062653 28.099960593721267 24.738386093962085 42-43 20.773676605805857 26.599238145277816 28.10543368798984 24.521651560926486 44-45 20.02605192871842 26.31901571872674 28.475414860545555 25.179517492009285 46-47 20.444196330837602 26.0048601077105 29.015061955427118 24.53588160602478 48-49 19.321117386925874 26.759052497920223 29.180349402338106 24.739480712815798 50-51 19.291562677875564 26.279609439992996 29.292000525417052 25.13682735671439 52-53 19.190857743333773 26.445991505757693 28.900126975787032 25.463023775121503 54-55 18.61946670169447 26.912299137440343 28.68448706160515 25.78374709926004 56-57 18.70703620999168 26.900258330049475 28.953763299619073 25.438942160339767 58-59 19.258724112264108 26.85428433819344 28.30355970051228 25.58343184903017 60-61 19.386794518148783 26.500722448443454 27.661018433381496 26.45146460002627 62-63 19.468890932177416 26.27413634572442 27.974079425544023 26.28289329655414 64-65 19.97022636717895 26.697753842112178 27.01957178510443 26.31244800560445 66-67 19.935198563860062 26.65834756337843 26.453653837733704 26.952800035027803 68-69 19.860764481807436 26.232540829283245 26.659442182232144 27.247252506677178 70-71 20.636849249091465 26.1570121283769 25.897587460046413 27.308551162485223 72-73 20.716756425412672 25.501335435001533 25.90743902972985 27.874469109855948 74-75 21.127238495555847 25.55825561539472 26.09352423486142 27.22098165418801 76 21.154603966898726 25.06020403695433 26.634265948596695 27.150926047550243 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 0.0 16 0.5 17 1.0 18 1.0 19 5.5 20 10.5 21 11.0 22 11.5 23 23.0 24 41.5 25 49.0 26 77.5 27 115.0 28 165.0 29 206.0 30 272.5 31 389.0 32 542.5 33 646.0 34 771.0 35 1012.5 36 1252.5 37 1376.0 38 1557.5 39 2054.0 40 2503.0 41 2795.5 42 2954.0 43 3047.0 44 3232.5 45 3331.5 46 3338.0 47 3411.5 48 3364.0 49 3046.5 50 2850.0 51 2668.0 52 2249.0 53 1802.5 54 1593.0 55 1508.0 56 1221.5 57 893.5 58 767.0 59 654.0 60 467.0 61 334.5 62 276.0 63 241.0 64 170.5 65 130.5 66 100.5 67 75.0 68 57.5 69 33.5 70 28.5 71 30.0 72 19.5 73 9.0 74 9.0 75 9.0 76 6.0 77 5.0 78 5.5 79 4.0 80 3.5 81 3.0 82 4.0 83 5.0 84 3.0 85 0.5 86 0.5 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 1.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7246376811594203 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 45678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.129602872279875 #Duplication Level Percentage of deduplicated Percentage of total 1 74.49938105293818 44.796181969438244 2 11.257554795019296 13.538245982748807 3 5.596009611883783 10.094575068960989 4 3.2440107769606055 7.802443189281492 5 1.7330517731012889 5.210385743684049 6 1.2269715284351561 4.426638644424012 7 0.6881234981431588 2.896361486930251 8 0.5352071652224568 2.5745435439380007 9 0.3058326658414039 1.6550637068172862 >10 0.9138571324546713 7.0055606637768735 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013135426244581637 0.0 2 0.0 0.0 0.0 0.013135426244581637 0.0 3 0.0 0.0 0.0 0.013135426244581637 0.0 4 0.0 0.0 0.0 0.019703139366872454 0.0 5 0.0 0.0 0.0 0.019703139366872454 0.0 6 0.0 0.0 0.0 0.019703139366872454 0.0 7 0.0 0.0 0.0 0.024081614781733 0.0 8 0.0 0.0 0.0 0.026270852489163273 0.0 9 0.0 0.0 0.0 0.03721704102631464 0.0 10 0.0 0.0 0.0 0.04597399185603573 0.0 11 0.0 0.0 0.0 0.05473094268575682 0.0 12 0.0 0.0 0.0 0.05473094268575682 0.0 13 0.0 0.0 0.0 0.05473094268575682 0.0 14 0.0 0.0 0.0 0.05473094268575682 0.0 15 0.0 0.0 0.0 0.07005560663776873 0.0 16 0.0 0.0 0.0 0.07443408205262927 0.0 17 0.0 0.0 0.0 0.07662331976005955 0.0 18 0.0 0.0 0.0 0.0810017951749201 0.0 19 0.0 0.0 0.0 0.08319103288235037 0.0 20 0.0 0.0 0.0 0.09851569683436227 0.0 21 0.0 0.0 0.0 0.10289417224922282 0.0 22 0.0 0.0 0.0 0.11165112307894391 0.0 23 0.0 0.0 0.0 0.12040807390866501 0.0 24 0.0 0.0 0.0 0.12259731161609527 0.0 25 0.0 0.0 0.0 0.1291650247383861 0.0 26 0.0 0.0 0.0 0.13792197556810717 0.0 27 0.0 0.0 0.0 0.1620035903498402 0.0 28 0.0 0.0 0.0 0.17294977888699156 0.0 29 0.0 0.0 0.0 0.19703139366872455 0.0 30 0.0 0.0 0.0 0.21892377074302727 0.0 31 0.0 0.0 0.0 0.24738386093962084 0.0 32 0.0 0.0 0.0 0.3021148036253776 0.0 33 0.0 0.0 0.0 0.34371032006655283 0.0 34 0.0 0.0 0.0 0.4290905906563335 0.0 35 0.0 0.0 0.0 0.5713910416393012 0.0 36 0.0 0.0 0.0 0.7859363369674679 0.0 37 0.0 0.0 0.0 1.131835894741451 0.0 38 0.0 0.0 0.0 1.6178466657909716 0.0 39 0.0 0.0 0.0 2.143263715574237 0.0 40 0.0 0.0 0.0 2.856955208196506 0.0 41 0.0 0.0 0.0 3.666973159945707 0.0 42 0.0 0.0 0.0 4.402557029642279 0.0 43 0.0 0.0 0.0 5.236656596173212 0.0 44 0.0 0.0 0.0 6.0970270151933095 0.0 45 0.0 0.0 0.0 6.917991155479662 0.0 46 0.0 0.0 0.0 7.706116730154561 0.0 47 0.0 0.0 0.0 8.524891632733482 0.0 48 0.0 0.0 0.0 9.291124830334077 0.0 49 0.0 0.0 0.0 10.090196593546127 0.0 50 0.0 0.0 0.0 10.84110512719471 0.0 51 0.0 0.0 0.0 11.657690792066203 0.0 52 0.0 0.0 0.0 12.465519506107974 0.0 53 0.0 0.0 0.0 13.205481851219405 0.0 54 0.0 0.0 0.0 13.866631638863348 0.0 55 0.0 0.0 0.0 14.464293532991812 0.0 56 0.0 0.0 0.0 15.138578746880336 0.0 57 0.0 0.0 0.0 15.744997591838521 0.0 58 0.0 0.0 0.0 16.29230701869609 0.0 59 0.0 0.0 0.0 16.83961644555366 0.0 60 0.0 0.0 0.0 17.474495380708436 0.0 61 0.0 0.0 0.0 18.13564516835238 0.0 62 0.0 0.0 0.0 18.755199439555145 0.0 63 0.0 0.0 0.0 19.34191514514646 0.0 64 0.0 0.0 0.0 19.89579228512632 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCAA 15 0.002211755 69.99562 48 GGGAGAA 15 0.002211755 69.99562 14 AGAACTC 15 0.002211755 69.99562 17 ATTGCTT 15 0.002211755 69.99562 20 TCTGTGC 25 2.3674448E-4 55.996494 24 TTGGTCA 25 2.3674448E-4 55.996494 21 GGGTATA 20 0.006792112 52.727722 1 TCGTTCG 20 0.006911275 52.49671 23 CTACTGG 20 0.006911275 52.49671 9 GGGTACA 20 0.006911275 52.49671 62 CGTTCGG 20 0.006911275 52.49671 24 TGGCCAA 20 0.006911275 52.49671 10 GGCCAAC 20 0.006911275 52.49671 11 GTGCTAG 20 0.006911275 52.49671 27 ACCACGT 20 0.006911275 52.49671 36 TTCGGTA 20 0.006911275 52.49671 26 CGGTAAG 20 0.006911275 52.49671 28 AAGGACT 20 0.006911275 52.49671 4 GTAACCA 20 0.006911275 52.49671 33 GACTATG 20 0.006911275 52.49671 7 >>END_MODULE