FastQCFastQC Report
Wed 25 May 2016
SRR1780626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45678
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT9302.0359910679101536No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT4280.9369937387801567Illumina PCR Primer Index 10 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1220.2670870003064933No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG950.2079775822058759No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC940.20578834449844563No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC610.13354350015324665Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC207.847472E-570.03
GATCCCC252.3667129E-455.99999630
TACTGAT252.3667129E-455.9999962
TGTGCAT200.006909562352.512
TATCTCA200.006909562352.546
CCTCATG200.006909562352.545
GCCGTAC200.006909562352.570
AAATGTC200.006909562352.545
CAAAGCT200.006909562352.566
GGGGCCA200.006909562352.57
GTACTGA305.8199494E-446.6666641
CTCTATT305.8199494E-446.66666423
TTGAAAA2300.042.60869658
TCTCGTA2400.042.29166438
CTCGTAT2500.042.039
TCGTATG2550.041.1764740
CTTGAAA2400.040.83333257
ATCTCGT2500.040.637
CGTATGC2500.040.641
TGAAAAA2500.040.659