##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 45678 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.88561232978677 34.0 34.0 34.0 31.0 34.0 2 33.363544813695874 34.0 34.0 34.0 33.0 34.0 3 33.53474320241692 34.0 34.0 34.0 34.0 34.0 4 33.50345899557774 34.0 34.0 34.0 34.0 34.0 5 33.54431017119839 34.0 34.0 34.0 34.0 34.0 6 37.32179605061518 38.0 38.0 38.0 37.0 38.0 7 36.92447129909365 38.0 37.0 38.0 35.0 38.0 8 37.00367791934848 38.0 37.0 38.0 36.0 38.0 9 36.78278383466877 38.0 37.0 38.0 34.0 38.0 10-11 36.52565786593108 38.0 37.0 38.0 34.0 38.0 12-13 36.41400674285214 38.0 37.0 38.0 34.0 38.0 14-15 36.651856473575904 38.0 37.0 38.0 34.5 38.0 16-17 36.79367529226323 38.0 37.0 38.0 34.5 38.0 18-19 36.78367047594028 38.0 37.0 38.0 34.0 38.0 20-21 36.55012259731161 38.0 37.0 38.0 34.0 38.0 22-23 36.60910066114979 38.0 37.0 38.0 34.0 38.0 24-25 35.98136958710977 38.0 36.5 38.0 32.5 38.0 26-27 36.20275625027365 38.0 37.0 38.0 34.0 38.0 28-29 36.071675642541265 38.0 37.0 38.0 33.5 38.0 30-31 35.79991461972941 38.0 36.5 38.0 32.5 38.0 32-33 36.27293226498533 38.0 37.0 38.0 34.0 38.0 34-35 36.30912036428916 38.0 37.0 38.0 34.0 38.0 36-37 36.41736722273305 38.0 37.0 38.0 34.0 38.0 38-39 36.05195061079732 38.0 37.0 38.0 32.5 38.0 40-41 35.668385218267 38.0 36.5 38.0 31.5 38.0 42-43 36.05164411751828 38.0 37.0 38.0 32.5 38.0 44-45 36.22667367222733 38.0 37.0 38.0 34.0 38.0 46-47 36.5074543543938 38.0 37.0 38.0 34.0 38.0 48-49 36.30940496519112 38.0 37.0 38.0 34.0 38.0 50-51 36.44137221419502 38.0 37.0 38.0 34.0 38.0 52-53 36.3748960112089 38.0 37.0 38.0 34.0 38.0 54-55 36.537567319059505 38.0 37.0 38.0 34.0 38.0 56-57 36.57810105521257 38.0 37.0 38.0 34.0 38.0 58-59 36.602040369543325 38.0 37.0 38.0 34.0 38.0 60-61 36.33120977275713 38.0 37.0 38.0 34.0 38.0 62-63 36.60468934716931 38.0 37.0 38.0 34.0 38.0 64-65 36.615361880993035 38.0 37.0 38.0 34.0 38.0 66-67 36.012237838784536 38.0 37.0 38.0 33.0 38.0 68-69 35.43976312448005 38.0 36.0 38.0 30.5 38.0 70-71 35.35699899295065 38.0 36.0 38.0 30.0 38.0 72-73 34.97890669468891 38.0 36.0 38.0 26.0 38.0 74-75 35.516276982354746 38.0 36.5 38.0 31.0 38.0 76 34.960484259380884 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7324177026507428 1101 2 0.505261953256344 1101 3 0.15539988106888813 1101 4 0.4254410171774978 1101 5 0.08096777714587233 1101 6 -1.5318145460807244 1101 7 -0.3693132426243295 1101 8 0.5100379178140386 1101 9 0.24161981216322204 1101 10-11 -0.055139951310522406 1101 12-13 0.7670006652343986 1101 14-15 0.3074933914414544 1101 16-17 -0.9778303770524133 1101 18-19 -1.120200755198212 1101 20-21 0.12445872143993597 1101 22-23 0.4445427855919917 1101 24-25 -1.4609818928054068 1101 26-27 -0.5098896812531208 1101 28-29 0.3292638499020697 1101 30-31 -0.06582970481165518 1101 32-33 -0.5205199292749825 1101 34-35 -0.07717202530683664 1101 36-37 -0.1378403106943864 1101 38-39 -0.7609535560103993 1101 40-41 0.7006003101057701 1101 42-43 0.3432924325624427 1101 44-45 0.20498984638668816 1101 46-47 0.20656464817865583 1101 48-49 -0.9061238445739832 1101 50-51 -1.3376783061765423 1101 52-53 -1.94584674953839 1101 54-55 -1.4895897080753997 1101 56-57 -0.48985620240173233 1101 58-59 -1.8644172948462483 1101 60-61 -1.991422451201835 1101 62-63 -0.4788732859469249 1101 64-65 -1.590849869050686 1101 66-67 -1.5608014427368317 1101 68-69 -1.375971790766016 1101 70-71 -0.5690983246807448 1101 72-73 -1.4263930332044552 1101 74-75 -0.9739352993395229 1101 76 -1.0466346586954174 1102 1 -0.16962806711347156 1102 2 0.11361840263220557 1102 3 0.18348587274711292 1102 4 0.3723030144035775 1102 5 0.05201493387264122 1102 6 0.4154808769428584 1102 7 0.49424431632157706 1102 8 0.4395628831399705 1102 9 0.45512536001342596 1102 10-11 0.607308037593782 1102 12-13 0.4385072533065184 1102 14-15 -0.5125482174351035 1102 16-17 0.10417378383524323 1102 18-19 -0.6662739174728287 1102 20-21 0.5075637838532501 1102 22-23 0.5831090130538499 1102 24-25 -4.599548120267265 1102 26-27 -1.392691345608192 1102 28-29 0.35336232424325686 1102 30-31 1.5643280621786388 1102 32-33 -0.02390065049551282 1102 34-35 0.6791126487569628 1102 36-37 -2.123190518738774 1102 38-39 -0.28687241870110114 1102 40-41 0.7152067594816458 1102 42-43 -0.13065867700760947 1102 44-45 0.5474395689941787 1102 46-47 0.7026204734213763 1102 48-49 -3.8034019305656557 1102 50-51 -1.11312040048999 1102 52-53 0.4557655944283283 1102 54-55 0.8922150769454049 1102 56-57 0.6238747268631712 1102 58-59 0.059429792532391446 1102 60-61 -5.944395752172703 1102 62-63 -2.861458237403234 1102 64-65 0.005630713473586013 1102 66-67 0.8041430787610864 1102 68-69 -0.15917220685477673 1102 70-71 -1.092503317745937 1102 72-73 -5.777337901443012 1102 74-75 -2.224152012238271 1102 76 -0.6036734936468733 1103 1 0.6855917131063052 1103 2 0.41609093010473686 1103 3 0.10491444417568374 1103 4 0.4111766407771995 1103 5 0.1292127360704427 1103 6 0.22427208573406432 1103 7 0.9088047558820165 1103 8 0.7201947512718405 1103 9 0.19344953583760116 1103 10-11 0.2108794661652098 1103 12-13 -0.7358746148253488 1103 14-15 -0.7889218438087298 1103 16-17 0.037964992626449146 1103 18-19 0.4107178407689247 1103 20-21 -0.5622851172456507 1103 22-23 0.6334524196472557 1103 24-25 -2.3229684499375978 1103 26-27 -0.47236167527851336 1103 28-29 -0.19306624718530685 1103 30-31 0.9321165237170987 1103 32-33 0.8822806681858069 1103 34-35 0.1583022091965205 1103 36-37 -0.5884377714860278 1103 38-39 -0.0587130780417624 1103 40-41 -0.18857071304583428 1103 42-43 0.14523418013525458 1103 44-45 1.0477829755875874 1103 46-47 0.5002166272675339 1103 48-49 -2.4752425899063155 1103 50-51 -0.6726671037866936 1103 52-53 0.6229359240986554 1103 54-55 0.10876727474760628 1103 56-57 0.26947912246757255 1103 58-59 -0.4650894382368378 1103 60-61 -4.219876521403471 1103 62-63 -1.3638620835570805 1103 64-65 -0.10686928652641114 1103 66-67 -0.025252525634513745 1103 68-69 -0.11947440465697667 1103 70-71 0.5050241547815375 1103 72-73 -4.9449890003441155 1103 74-75 -1.9321877265239848 1103 76 -3.509442724416104 1104 1 -0.010655941677931935 1104 2 0.37944302516415007 1104 3 0.2854022490537318 1104 4 -0.1978915268275543 1104 5 -0.48529633209716394 1104 6 0.13208947160023143 1104 7 0.6102431548813883 1104 8 0.4622835567752759 1104 9 0.7603663588519893 1104 10-11 -0.20700671269657533 1104 12-13 0.22244308373625188 1104 14-15 0.5916910392431731 1104 16-17 0.40309945166335126 1104 18-19 0.6465527375794338 1104 20-21 0.07220518919587704 1104 22-23 0.37488456473793974 1104 24-25 0.45826982398359917 1104 26-27 0.8157871677483755 1104 28-29 1.2240069235463906 1104 30-31 0.7205780621786388 1104 32-33 0.6569210684359703 1104 34-35 0.27068494840852964 1104 36-37 1.124414010877949 1104 38-39 1.508641518581122 1104 40-41 1.6804724389241557 1104 42-43 0.4777545053385026 1104 44-45 1.0058817873449328 1104 46-47 0.8268582782994258 1104 48-49 0.3965835514320162 1104 50-51 0.8064135716354457 1104 52-53 0.33519358513680686 1104 54-55 0.14522756242740797 1104 56-57 0.1273590474206685 1104 58-59 -0.022125085516385923 1104 60-61 0.5517932722175445 1104 62-63 0.6245681853609923 1104 64-65 0.9146754289207379 1104 66-67 0.48750544809907126 1104 68-69 1.5713010800209446 1104 70-71 1.8103712467139985 1104 72-73 1.6615776037834706 1104 74-75 0.4109017043703176 1104 76 0.021326506353126717 1105 1 0.8731208506354449 1105 2 0.5102634242772268 1105 3 0.33067201993326023 1105 4 -0.5262359466353885 1105 5 -0.2364049795472738 1105 6 0.09513455659653403 1105 7 0.8898070868843462 1105 8 -0.7760756449985564 1105 9 0.8000928924809614 1105 10-11 0.04724310252885289 1105 12-13 -0.6214224003731346 1105 14-15 -0.021206226093120506 1105 16-17 0.49973655439801234 1105 18-19 0.521090801141888 1105 20-21 0.09022071026017642 1105 22-23 0.08811442430926775 1105 24-25 0.7136282866591444 1105 26-27 0.5975683946515531 1105 28-29 0.6152670861480161 1105 30-31 1.1789113955119745 1105 32-33 0.7783179642231062 1105 34-35 -0.8575486066542908 1105 36-37 0.15684194879369784 1105 38-39 -0.0999135442422272 1105 40-41 0.35711693264181577 1105 42-43 0.2861432710443452 1105 44-45 0.9047177075223161 1105 46-47 0.5615219885728919 1105 48-49 -0.533109722773446 1105 50-51 0.6045473484277579 1105 52-53 0.3386119147746456 1105 54-55 0.8766577176380537 1105 56-57 0.17419940218785257 1105 58-59 0.5528010046536025 1105 60-61 0.6985966720697192 1105 62-63 -0.3586173283123273 1105 64-65 0.1666155619584302 1105 66-67 0.4037101783281898 1105 68-69 0.10733212214955046 1105 70-71 0.16994256969995547 1105 72-73 0.6987172933621792 1105 74-75 -0.24052105985731487 1105 76 0.7031446881713066 1106 1 -1.586608016988162 1106 2 -0.3158928755632786 1106 3 0.18035304066690827 1106 4 0.33141955575695903 1106 5 0.02475929477489558 1106 6 0.1661074433588965 1106 7 -1.1169712225255424 1106 8 0.06283982549586398 1106 9 -1.162825767806119 1106 10-11 0.03807244862134951 1106 12-13 1.0899013635822072 1106 14-15 -0.19863844299901956 1106 16-17 -0.007041755011876205 1106 18-19 0.20849112012115256 1106 20-21 0.07766090164773232 1106 22-23 0.6630745519009693 1106 24-25 -0.9821452380617544 1106 26-27 0.43249662431662017 1106 28-29 0.6525477879024066 1106 30-31 0.466192097266358 1106 32-33 -0.5538191968614257 1106 34-35 0.7788150297093495 1106 36-37 0.7842342932913056 1106 38-39 -0.2911017420093742 1106 40-41 0.3794454812861545 1106 42-43 0.5724271625913886 1106 44-45 0.29845773942025033 1106 46-47 0.5990020523687463 1106 48-49 0.5300880443716807 1106 50-51 0.1673463914899571 1106 52-53 0.4014109578368448 1106 54-55 0.15795755814841073 1106 56-57 0.20031582962007377 1106 58-59 0.575328915339405 1106 60-61 0.6975998618623862 1106 62-63 0.6050987801406293 1106 64-65 -0.6182727952983456 1106 66-67 0.09330347976359832 1106 68-69 1.3585282943983543 1106 70-71 0.06946410080043108 1106 72-73 0.07032813865723853 1106 74-75 0.3581631506690002 1106 76 0.25816861161628424 1107 1 0.6884689885026631 1107 2 -0.06318094306026723 1107 3 0.18430375715278302 1107 4 0.10683136772964019 1107 5 0.05569541369816022 1107 6 0.3653173000617258 1107 7 0.6206074569976963 1107 8 -0.1328880437956883 1107 9 -0.5238141165405565 1107 10-11 0.6017191608217018 1107 12-13 0.4725516916925585 1107 14-15 0.4375608338189849 1107 16-17 0.7610712756563984 1107 18-19 0.603730171949195 1107 20-21 0.8695117118794258 1107 22-23 0.16595388764491048 1107 24-25 0.2603550967447319 1107 26-27 -0.22529767058092176 1107 28-29 0.9947200123566731 1107 30-31 0.5526391308809266 1107 32-33 0.8547116722962045 1107 34-35 0.17321706533275005 1107 36-37 0.8557489578152087 1107 38-39 0.6094607328801374 1107 40-41 0.4527681008120652 1107 42-43 0.8803486839520431 1107 44-45 0.7104371153409588 1107 46-47 1.0234697100625993 1107 48-49 0.7742289309392518 1107 50-51 0.9898989561076093 1107 52-53 0.6686200110041085 1107 54-55 1.0395365055168355 1107 56-57 0.8741473549983994 1107 58-59 0.8893439146697943 1107 60-61 1.2600412707280597 1107 62-63 0.9746581748105072 1107 64-65 0.6263368203438162 1107 66-67 -0.08966826256933302 1107 68-69 1.4318174332760805 1107 70-71 0.5633036615343343 1107 72-73 1.344969895721654 1107 74-75 0.10140005973555333 1107 76 0.9755249796355727 1108 1 -0.22094977689975082 1108 2 0.3480109785602181 1108 3 0.07971759378197874 1108 4 0.28997736760942416 1108 5 0.11261673364766978 1108 6 0.016155792578402384 1108 7 -0.21512013812161968 1108 8 -0.2383758120231363 1108 9 -0.13029365011031047 1108 10-11 0.04473773388174607 1108 12-13 1.0246644524066255 1108 14-15 0.42755301991022776 1108 16-17 -0.6288127178520426 1108 18-19 0.1599001545181693 1108 20-21 0.7314459548318837 1108 22-23 -0.19940645376279065 1108 24-25 0.8443846693840271 1108 26-27 -0.8117014693427151 1108 28-29 -1.2781056172640533 1108 30-31 0.14971192044635728 1108 32-33 0.9715296082221485 1108 34-35 0.4979751346962189 1108 36-37 0.9504174677629109 1108 38-39 0.9720561527274683 1108 40-41 1.0669713826537475 1108 42-43 0.14995858958406671 1108 44-45 0.8397272517838346 1108 46-47 0.762905906492243 1108 48-49 0.8787549873196099 1108 50-51 0.6234867423671488 1108 52-53 -0.30189751018360056 1108 54-55 -0.09038475432568305 1108 56-57 0.4180254580217806 1108 58-59 0.3160734933197986 1108 60-61 0.9643148777485564 1108 62-63 0.9035516051164549 1108 64-65 0.9116346504814601 1108 66-67 1.0166008965338733 1108 68-69 1.2454256559123777 1108 70-71 -0.7973683346188238 1108 72-73 -0.527302749586994 1108 74-75 -1.335337332823201 1108 76 0.6512477661956453 1109 1 -1.352300024785432 1109 2 0.2314755454893529 1109 3 0.18639592565716612 1109 4 0.2530569826575402 1109 5 0.2243694312271387 1109 6 -0.24695298549100642 1109 7 -5.969535196967968E-4 1109 8 0.13671896779605675 1109 9 0.4096888520769184 1109 10-11 0.18865724394299122 1109 12-13 0.4753789464282079 1109 14-15 0.5543829994961058 1109 16-17 0.7241473288087832 1109 18-19 0.23977766982875437 1109 20-21 0.5475439425834097 1109 22-23 -0.23848490229006813 1109 24-25 -0.7261023530714965 1109 26-27 0.13005997714314077 1109 28-29 -0.9449180990371602 1109 30-31 -0.016458974858402087 1109 32-33 0.48185331775845697 1109 34-35 -0.13970348880917527 1109 36-37 0.7296533966051442 1109 38-39 0.6090931897645007 1109 40-41 -0.16948239395750875 1109 42-43 0.5785675134685846 1109 44-45 0.4851049129095273 1109 46-47 0.6834075104584088 1109 48-49 0.7018229371592071 1109 50-51 0.5308941497745536 1109 52-53 -0.37393017276744445 1109 54-55 -0.9493523833720516 1109 56-57 -0.4782416752532228 1109 58-59 -0.7269128000601981 1109 60-61 0.43824313671618853 1109 62-63 0.2934028737078762 1109 64-65 0.6209084912513632 1109 66-67 0.16961926923728043 1109 68-69 0.1330896979071241 1109 70-71 -0.056656756899371885 1109 72-73 -0.9307439860990954 1109 74-75 -0.932187726523992 1109 76 -0.36385867883205947 1110 1 -1.5247867972722062 1110 2 -1.098683184669376 1110 3 -0.15381571455447585 1110 4 0.12924745884802036 1110 5 0.3714593783170841 1110 6 0.050401511863491066 1110 7 0.22638717346443116 1110 8 0.5774597085368001 1110 9 0.8588952012832678 1110 10-11 -0.7161046608189139 1110 12-13 0.5560667771160439 1110 14-15 0.5072930524061618 1110 16-17 0.45139600605746466 1110 18-19 0.290571320622405 1110 20-21 0.7134169584564205 1110 22-23 -0.7199663837715491 1110 24-25 -0.5226631996323476 1110 26-27 0.9327054797886447 1110 28-29 0.25217184805278237 1110 30-31 -0.2952949536943734 1110 32-33 0.08423427013941165 1110 34-35 0.21135471224902602 1110 36-37 0.44526186221360575 1110 38-39 0.5593577400290499 1110 40-41 -0.9430273675024878 1110 42-43 -0.241273756372685 1110 44-45 0.7364276642322736 1110 46-47 0.36779904484994574 1110 48-49 0.7531456884819647 1110 50-51 -0.9519100830296736 1110 52-53 0.41310686426959364 1110 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-1.0545183036239933 2116 36-37 0.5878286450242882 2116 38-39 -2.95477311556521 2116 40-41 -0.8479828456286924 2116 42-43 -0.9246845190517377 2116 44-45 -0.07948406631360427 2116 46-47 -0.9211092013753728 2116 48-49 0.0022746083425886354 2116 50-51 -0.4903343958442292 2116 52-53 -0.07537552055425323 2116 54-55 0.14929260307781078 2116 56-57 0.3346761205913964 2116 58-59 -1.225377118036711 2116 60-61 -1.2164994107092895 2116 62-63 0.37660070568619375 2116 64-65 -1.4308530263638133 2116 66-67 0.0891314643592267 2116 68-69 -0.8677233102229565 2116 70-71 0.9485826288278218 2116 72-73 0.767268660694036 2116 74-75 -0.10129341758089083 2116 76 -0.929893005841997 2117 1 0.5468897900293825 2117 2 0.5064755454893515 2117 3 0.3330394441756823 2117 4 -0.2783666284535684 2117 5 0.3265685053012106 2117 6 0.3808826626571431 2117 7 0.6315211020358618 2117 8 0.1929110974256858 2117 9 -0.29933892570085874 2117 10-11 -0.6399017838347874 2117 12-13 -1.361715960979197 2117 14-15 -0.17828482457796468 2117 16-17 -1.231205680450472 2117 18-19 -1.12497927461569 2117 20-21 -0.028150501861034627 2117 22-23 0.22317597733956518 2117 24-25 -1.5247713345529803 2117 26-27 -2.2046333098939 2117 28-29 -1.4993162471853125 2117 30-31 0.18932806217863885 2117 32-33 -1.3737890433526516 2117 34-35 0.32699211304267806 2117 36-37 -0.7632574830244891 2117 38-39 0.19471240272746826 2117 40-41 -0.3216235976612154 2117 42-43 0.23820293013525173 2117 44-45 -0.06639971672010603 2117 46-47 -0.9497232765786237 2117 48-49 -0.5093171091370863 2117 50-51 -0.15385700763285115 2117 52-53 0.2364575587140365 2117 54-55 -0.4057759944831645 2117 56-57 -0.49554491599396755 2117 58-59 -1.1632264574676086 2117 60-61 -0.6240832521727029 2117 62-63 -0.1856769874032338 2117 64-65 -1.263900536526414 2117 66-67 -0.5965265640960524 2117 68-69 0.048975114573792666 2117 70-71 -0.9775479606030757 2117 72-73 -0.12443611572872726 2117 74-75 -0.24703147652398627 2117 76 0.12288900635312672 2118 1 -0.5294852099706162 2118 2 0.14347554548935193 2118 3 -0.879085555824318 2118 4 0.23794587154642954 2118 5 0.21844350530120948 2118 6 -0.2508048373428551 2118 7 0.6989586020358587 2118 8 0.310348597425687 2118 9 -1.7520889257008605 2118 10-11 -0.24112053383478838 2118 12-13 -1.12402846097919 2118 14-15 0.24030892542203475 2118 16-17 -2.5471119304504697 2118 18-19 -0.6246667746156902 2118 20-21 -0.35690050186103406 2118 22-23 0.7071447273395677 2118 24-25 0.5804161654470192 2118 26-27 -2.630977059893901 2118 28-29 -0.7050662471853144 2118 30-31 -0.5474219378213618 2118 32-33 -0.22925779335265162 2118 34-35 -0.07985163695732211 2118 36-37 -2.427976233024488 2118 38-39 0.0680561527274719 2118 40-41 -0.12518609766121358 2118 42-43 0.5732341801352518 2118 44-45 -2.016524716720106 2118 46-47 0.10637047342137862 2118 48-49 0.22952664086291463 2118 50-51 -0.4485132576328539 2118 52-53 -1.3764486912859653 2118 54-55 -1.1084634944831606 2118 56-57 -0.6549824159939703 2118 58-59 -1.4303202074676094 2118 60-61 -1.0146457521727044 2118 62-63 -1.1297082374032286 2118 64-65 -0.19686928652641456 2118 66-67 -0.387714064096059 2118 68-69 -2.1762436354262036 2118 70-71 -0.5443604606030732 2118 72-73 0.4956263842712758 2118 74-75 0.12781227347601032 2118 76 -0.810673493646874 2119 1 0.8501254094984105 2119 2 -1.0056925961035716 2119 3 -0.1322536974172408 2119 4 -0.9624789072146314 2119 5 -0.19182198142445372 2119 6 -0.5465924479623254 2119 7 -0.10167856610573267 2119 8 0.30263178326639206 2119 9 -0.7946375982672294 2119 10-11 -0.29266035684364056 2119 12-13 -0.029373593722560543 2119 14-15 0.15464520860787445 2119 16-17 -0.3457313994770175 2119 18-19 -0.23829509319975983 2119 20-21 -1.5809712983212094 2119 22-23 -0.2681473080586656 2119 24-25 0.8225046610222364 2119 26-27 0.41072205515035165 2119 28-29 0.21224348732796017 2119 30-31 0.9108435489043032 2119 32-33 -0.0920896517597285 2119 34-35 0.1740952656975452 2119 36-37 -0.48404702948467104 2119 38-39 -0.943873050812357 2119 40-41 -1.5714161861567888 2119 42-43 0.2638182509317133 2119 44-45 0.7050328054037891 2119 46-47 -0.002302092950301926 2119 48-49 -0.3475707042698275 2119 50-51 0.5084424945795405 2119 52-53 0.2093212202184631 2119 54-55 -0.09320685731502465 2119 56-57 -1.271194805374499 2119 58-59 -0.3838777295915037 2119 60-61 0.043159557561814665 2119 62-63 -0.17700027280146458 2119 64-65 -0.21173654316358181 2119 66-67 0.12035673236412237 2119 68-69 -0.3781197416208997 2119 70-71 -0.559475504850866 2119 72-73 0.4599803665721538 2119 74-75 -0.06758595661248279 2119 76 0.1894680992734834 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 3.0 23 10.0 24 7.0 25 38.0 26 72.0 27 108.0 28 195.0 29 336.0 30 556.0 31 973.0 32 1408.0 33 2257.0 34 3730.0 35 5656.0 36 10221.0 37 20106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.358378212706334 23.536494592582862 11.423442357371163 29.68168483733964 2 17.113271158982442 19.486404833836858 39.66241954551425 23.73790446166645 3 16.67761285520382 25.34480493892027 31.483427470554755 26.494154735321164 4 12.231271071412934 21.045142081527214 38.90932177415824 27.814265072901616 5 11.986076448180743 37.93073251893691 37.197337886947764 12.885853145934586 6 25.892114365777836 40.794255440255704 18.015237094443716 15.298393099522748 7 25.42142825868033 30.172074083804016 23.76636455186304 20.640133105652612 8 21.868295459520994 37.68553789570472 20.52848198257367 19.91768466220062 9 22.48784973072376 15.103550943561453 22.247033582906433 40.16156574280835 10-11 23.59779324839091 26.261000919479837 28.48636104908271 21.654844783046542 12-13 23.4379788957485 22.94211655501554 27.167345330355968 26.452559218879983 14-15 21.09221069223696 24.72962914313236 24.099128683392443 30.079031481238232 16-17 22.872060948377776 27.789088839266167 25.210166819913304 24.128683392442753 18-19 22.60497394807128 27.011909453128418 26.512763255834322 23.87035334296598 20-21 20.990411138841456 27.416918429003022 25.397346643898594 26.19532378825693 22-23 22.014974385918823 27.833968212268488 26.41315294014624 23.73790446166645 24-25 22.746179780200535 28.832260606856693 25.65567669337537 22.765882919567407 26-27 22.460484259380884 28.903410832348175 26.329961907263886 22.30614300100705 28-29 21.114103069311266 28.14374534786987 27.840535925390782 22.901615657428085 30-31 23.06690310433907 27.407066859319585 27.72560094575069 21.800429090590654 32-33 22.34883313630194 28.14374534786987 27.647883007136915 21.859538508691273 34-35 21.168834011997024 28.255396470948817 29.16611935723981 21.409650159814355 36-37 21.49393581155042 27.463987039712773 28.464468672008408 22.577608476728404 38-39 21.38556854503262 26.814878059459694 27.670870003064934 24.128683392442753 40-41 20.766014273829853 25.616270414641622 29.587547615920133 24.03016769560839 42-43 22.750558255615395 25.37107579140943 29.467139542011473 22.411226410963703 44-45 19.475458645299707 27.247252506677178 29.398178554227417 23.879110293795698 46-47 22.294102193616183 25.055825561539475 28.683392442751433 23.966679802092912 48-49 20.876570778055083 25.470686107097507 28.853058365077278 24.79968474977013 50-51 18.795700337142605 25.50461929156268 30.470905030868252 25.228775340426463 52-53 19.150356845746312 25.495862340732955 31.90047725382022 23.45330355970051 54-55 18.230877008625594 25.922763693681862 30.76645212137134 25.079907176321203 56-57 20.42668242917816 25.283506283112224 28.943911729935635 25.345899557773983 58-59 18.67419764438023 26.722930075747627 30.03634134594334 24.566530933928806 60-61 19.975699461447523 28.34406059809974 27.443189281492188 24.23705065896055 62-63 18.02508866412715 30.10420771487368 26.67695608389159 25.19374753710758 64-65 18.721266255089976 30.663557949122115 25.484916152195808 25.1302596435921 66-67 18.54065414422698 30.57927229738605 25.066771750076622 25.813301808310346 68-69 18.724550111651123 30.215858837952624 25.24081614781733 25.818774902578923 70-71 19.421822321467666 30.258548973247517 24.51617846665791 25.803450238626912 72-73 19.506107973203733 28.812557467489817 24.58842331100311 27.09291124830334 74-75 19.732694075922762 29.551425193747537 24.370594159113796 26.345286571215905 76 19.781951924339943 29.412408599325712 24.89382197118963 25.911817505144707 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 3.0 19 3.0 20 4.5 21 7.0 22 9.0 23 21.5 24 36.5 25 41.0 26 81.5 27 131.0 28 186.0 29 232.0 30 267.5 31 367.0 32 530.0 33 629.0 34 740.5 35 989.5 36 1301.5 37 1476.0 38 1770.5 39 2432.0 40 3030.5 41 3264.5 42 3267.0 43 3259.0 44 3319.5 45 3475.0 46 3562.0 47 3531.5 48 3303.5 49 2987.5 50 2869.0 51 2588.5 52 2004.0 53 1558.5 54 1417.0 55 1346.0 56 1011.0 57 683.5 58 620.0 59 483.0 60 312.0 61 226.5 62 175.0 63 140.5 64 94.0 65 79.0 66 55.0 67 34.0 68 28.0 69 13.0 70 4.0 71 4.0 72 2.0 73 1.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 45678.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.78646175401725 #Duplication Level Percentage of deduplicated Percentage of total 1 57.55218993041342 24.624545733175708 2 16.915677445763404 14.475239721528963 3 9.583503888661483 12.301326678050703 4 5.270159639787146 9.019659354612724 5 3.20814572247237 6.863260212793905 6 2.0159639787146952 5.175357940365164 7 1.4940646745804338 4.474801873987477 8 1.0437986082685222 3.572835938526205 9 0.6907490790012281 2.6599238145277813 >10 2.195047073270569 13.045667498576996 >50 0.015349979533360621 0.5473094268575682 >100 0.010233319688907082 1.20408073908665 >500 0.005116659844453541 2.0359910679101536 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT 930 2.0359910679101536 No Hit ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT 428 0.9369937387801567 Illumina PCR Primer Index 10 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC 122 0.2670870003064933 No Hit TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG 95 0.2079775822058759 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 94 0.20578834449844563 No Hit CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC 61 0.13354350015324665 Illumina PCR Primer Index 10 (95% over 21bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.013135426244581637 0.0 2 0.0 0.0 0.0 0.013135426244581637 0.0 3 0.0 0.0 0.0 0.013135426244581637 0.0 4 0.0 0.0 0.0 0.024081614781733 0.0 5 0.0 0.0 0.0 0.024081614781733 0.0 6 0.0 0.0 0.0 0.024081614781733 0.0 7 0.0 0.0 0.0 0.028460090196593547 0.0 8 0.0 0.0 0.0 0.030649327904023818 0.0 9 0.0 0.0 0.0 0.04597399185603573 0.0 10 0.0 0.0 0.0 0.056920180393187095 0.0 11 0.0 0.0 0.0 0.06786636893033846 0.0 12 0.0 0.0 0.0 0.06786636893033846 0.0 13 0.0 0.0 0.0 0.06786636893033846 0.0 14 0.0 0.0 0.0 0.06786636893033846 0.0 15 0.0 0.0 0.0 0.08319103288235037 0.0 16 0.0 0.0 0.0 0.08538027058978064 0.0 17 0.0 0.0 0.0 0.08756950829721091 0.0 18 0.0 0.0 0.0 0.09413722141950173 0.0 19 0.0 0.0 0.0 0.09851569683436227 0.0 20 0.0 0.0 0.0 0.11384036078637419 0.0 21 0.0 0.0 0.0 0.11821883620123473 0.0 22 0.0 0.0 0.0 0.12478654932352555 0.0 23 0.0 0.0 0.0 0.1357327378606769 0.0 24 0.0 0.0 0.0 0.13792197556810717 0.0 25 0.0 0.0 0.0 0.144489688690398 0.0 26 0.0 0.0 0.0 0.1532466395201191 0.0 27 0.0 0.0 0.0 0.1773282543018521 0.0 28 0.0 0.0 0.0 0.19484215596129428 0.0 29 0.0 0.0 0.0 0.21892377074302727 0.0 30 0.0 0.0 0.0 0.24519462323219055 0.0 31 0.0 0.0 0.0 0.27584395113621435 0.0 32 0.0 0.0 0.0 0.3349533692368317 0.0 33 0.0 0.0 0.0 0.37873812338543716 0.0 34 0.0 0.0 0.0 0.4728753448049389 0.0 35 0.0 0.0 0.0 0.6283112220324882 0.0 36 0.0 0.0 0.0 0.8625596567275274 0.0 37 0.0 0.0 0.0 1.2194054030386619 0.0 38 0.0 0.0 0.0 1.6988484609658916 0.0 39 0.0 0.0 0.0 2.239590174701169 0.0 40 0.0 0.0 0.0 2.9970664214720433 0.0 41 0.0 0.0 0.0 3.8640045536144316 0.0 42 0.0 0.0 0.0 4.623670038092736 0.0 43 0.0 0.0 0.0 5.499365121064845 0.0 44 0.0 0.0 0.0 6.396952581111257 0.0 45 0.0 0.0 0.0 7.246376811594203 0.0 46 0.0 0.0 0.0 8.095801042077149 0.0 47 0.0 0.0 0.0 8.938657559437804 0.0 48 0.0 0.0 0.0 9.737729322649853 0.0 49 0.0 0.0 0.0 10.569639651473357 0.0 50 0.0 0.0 0.0 11.39279302946714 0.0 51 0.0 0.0 0.0 12.237838784535224 0.0 52 0.0 0.0 0.0 13.122290818337055 0.0 53 0.0 0.0 0.0 13.914794868426814 0.0 54 0.0 0.0 0.0 14.624107885634222 0.0 55 0.0 0.0 0.0 15.272122247033582 0.0 56 0.0 0.0 0.0 15.96611060028898 0.0 57 0.0 0.0 0.0 16.63601733876264 0.0 58 0.0 0.0 0.0 17.224922282061385 0.0 59 0.0 0.0 0.0 17.80944874994527 0.0 60 0.0 0.0 0.0 18.47497701300407 0.0 61 0.0 0.0 0.0 19.175533079381758 0.0 62 0.0 0.0 0.0 19.82354744078112 0.0 63 0.0 0.0 0.0 20.443101711983886 0.0 64 0.0 0.0 0.0 21.010114278208327 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGATC 20 7.847472E-5 70.0 3 GATCCCC 25 2.3667129E-4 55.999996 30 TACTGAT 25 2.3667129E-4 55.999996 2 TGTGCAT 20 0.0069095623 52.5 12 TATCTCA 20 0.0069095623 52.5 46 CCTCATG 20 0.0069095623 52.5 45 GCCGTAC 20 0.0069095623 52.5 70 AAATGTC 20 0.0069095623 52.5 45 CAAAGCT 20 0.0069095623 52.5 66 GGGGCCA 20 0.0069095623 52.5 7 GTACTGA 30 5.8199494E-4 46.666664 1 CTCTATT 30 5.8199494E-4 46.666664 23 TTGAAAA 230 0.0 42.608696 58 TCTCGTA 240 0.0 42.291664 38 CTCGTAT 250 0.0 42.0 39 TCGTATG 255 0.0 41.17647 40 CTTGAAA 240 0.0 40.833332 57 ATCTCGT 250 0.0 40.6 37 CGTATGC 250 0.0 40.6 41 TGAAAAA 250 0.0 40.6 59 >>END_MODULE