FastQCFastQC Report
Wed 25 May 2016
SRR1780625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTA11.0309278350515463No Hit
CGCCGGTGGCGGCGGCCATGATGTCCACCCGCGGGCCGCGCAGGAGGGCG11.0309278350515463No Hit
GTGGTGGAGCCGTTCATGCACTCCACGCTCCAGCTCGCACGCTGTCTCTT11.0309278350515463No Hit
GGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAA11.0309278350515463No Hit
TGGTTACTAGCACAGAGAGTTCTCCTAGTAGGTTAATAGTGGGGGGTAAG11.0309278350515463No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG11.0309278350515463No Hit
AGTCCGAGGAGGTTAGTTGTGGCAATAAAAATGATTAAGGATACTAGTAT11.0309278350515463No Hit
CGCCGCGTGCGGCCTCGGCGGCCACGTTCCTGGCGCTGTCTCTTATACAC11.0309278350515463No Hit
GTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTTGATT11.0309278350515463No Hit
CAGGGAGGCCGGCAGGGCGGTGTTCCCGTCCACCCGCCCCTGCCTCTTAT11.0309278350515463No Hit
GAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTG11.0309278350515463No Hit
CTATACAACCGTATCGGCGATATCGGTTTCATCCTCGCCTTAGCATGATT11.0309278350515463No Hit
GTGCTAATGGTGGAGTTAAAGACTTTTTCTCTGATTTGTCCTTGGAAAAA11.0309278350515463No Hit
CTAAATGGTCGCTATGGGTTTTTAAATTTTTTCTTACCTGTCGTTCAAAT11.0309278350515463No Hit
CCCGCCAGCCAGCGCAAAGCCCAGGGCCGGCTCGTTGAAGGCTGTCTCTT11.0309278350515463No Hit
CAGCCTCACTGTCCACTTCTTAGGCCTCACCTATACTTCTCCATAACAAC11.0309278350515463No Hit
TCTTCTGAATCTTACCCCCCTCTCTTCTACACCAACGCCTTTCTCTTGTA11.0309278350515463No Hit
CGACCCGGTCCCTCCGTCGGGCCGCGTTGCCGTCGACCGACTCGGGGCCG11.0309278350515463No Hit
CTATCTCCCTAATTGAAAACAAAATACTCAAATGGGCCTGTCCTTGTAGT11.0309278350515463No Hit
CCCAGGCGGCGCGGTCGGCGTCGCCGGCCGGGGCGTCGGGCCAGCCGGCG11.0309278350515463No Hit
CAACAGAGGCTTACGACCCCTTATTTACCGAGAAAGCTCACAAGAACTGC11.0309278350515463No Hit
GTGCAGGGCCTGCATCTGGGCTGGGTCTCTGTCTCTTATACACATCTGAC11.0309278350515463No Hit
GCGCTCACCGGCGGCCCCATGACCGGGGCGGCCCGGCTGGGCACCCCCGA11.0309278350515463No Hit
CCTCGGCCAGGCTGGCCAGGCGGGGATTCACGTCCTCCAGGACCGGCTCC11.0309278350515463No Hit
CTCGAGGGCCTCGACGCCGTCGATCTCGGTGAGCAGCTGCGGGACGATCG11.0309278350515463No Hit
CGCATGAACCCCGCCCCCGTCACTGAAGACACGCGGGACGAGGAGACTCC11.0309278350515463No Hit
GCACATACCAAGGCCACCACACACCACCTGTCCAAAAAGGCCTTCGATAC11.0309278350515463No Hit
GACGTGTTCGCCGAGGGTCTCAGCCACGAGCTCGGACTCCTCCATGCCTG11.0309278350515463No Hit
ATTGAGGAGTATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACC11.0309278350515463No Hit
CGTCGCCGTCGGGCGGCGCCGTCGTCGTCGCCGCCCCTGGCCTGTCTCTT11.0309278350515463No Hit
GCCATACACTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCA11.0309278350515463No Hit
ATGTAATTATTATACGAATGGGGGCTTCAATCGGGAGTACTACTCGATTG11.0309278350515463No Hit
GAACGGCAGCGCGGCGGCGGTGTTGAGCACCGTCACGGTCTCCGCGATCA11.0309278350515463No Hit
ATGCTCACCCTCTCCACCGCCCGCTGGGCCGCCGAGGAGGGCCCTGTCTC11.0309278350515463No Hit
CCCCGTCGTCGCGCGCCGCGGGCCTGTCTCTTATACACATCTGACGCTGC11.0309278350515463No Hit
AGGTCACCCTGCTCTCGGCGCTGATCACCCTGACGCCCGGCACGTTGACG11.0309278350515463No Hit
ATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTG11.0309278350515463No Hit
GCACGGCGGTGCCCCCGATGCCGCTCAGGGAACCGGTGTGGCCGGTGTCT11.0309278350515463No Hit
TTTTCGCTTCCCACAGGTCTCCTGTCTCTTATACACATCTGACGCTGCCG11.0309278350515463No Hit
CGTCAGTACATGGTTCTCCCTGAGCACAGTGAGCTCTGTCATTGCTGTTT11.0309278350515463No Hit
TCTGTGAGTTGAACGCACACATCACAAAAAGTTTCGGACAATGCTTCCAT11.0309278350515463No Hit
GCAGGAAGTTGAACCAGGCAACGCCCGCACCGGATAGCGCCTGTTTCTTA11.0309278350515463No Hit
GTTATACACATCTGACGCCGCCCACCGGAACGGCCTTGTGGCGCCTGTCT11.0309278350515463No Hit
AGGTTTCACCGTGTTGGCCACGCTGGTGTCGAACTCCTGACCTCAGGTGA11.0309278350515463No Hit
AGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGCTGTCTCTTA11.0309278350515463No Hit
CTGCAGGGCGTCCCCGTTGACCACCTCGCCGTCCAGCGCGTGCGCCAGGG11.0309278350515463No Hit
ACATGATGTCCCGCATGGGCCTGCGCCCGGACCGCGGCCTGTCTCTTATA11.0309278350515463No Hit
GGCCCCAAACTGACCCTCCTCCTCCTTAATATACCTTGTATCGACCCGCT11.0309278350515463No Hit
GGGGGGGAGTGGATGTAGGTGGCACCCGTCCGGGATGACGCCGGTCGGAC11.0309278350515463No Hit
TGTATCCCGTGGCCGGCGTCATGACGGTCGCTGCGCCGTCCGGCAACGCG11.0309278350515463No Hit
GTGCTACTCTCCTCGCGCCGGGCCCATAACACTTGGGGGACGCTAAAGTG11.0309278350515463No Hit
GTTGATATCGGTGGCAAATTCAATCCATGAACCCCTGAAGGGGATAATGC11.0309278350515463No Hit
GTTGGATGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAA11.0309278350515463No Hit
GTAAATAATAGGAGCTTAAACCCCCTTATTTCTAGGACTATGACTGTCTC11.0309278350515463No Hit
GGTTAGGATGAGTGGGAAGAAGAAAGAGAGGAAGTAAAGTTTAATTATGC11.0309278350515463No Hit
AGCCAGCGGGCAGCTCTCGTCCGGCCAGTCGGTCGTACAGCCGCGCGTTC11.0309278350515463No Hit
CAACCATCCCTGAGAATCCAAAGTTCTCGGTGCCACCTCTTGCTCTTGTA11.0309278350515463No Hit
GCTATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCT11.0309278350515463No Hit
GTCGCCGGATGGGACAAGGGGTGCGTAGGGGGGGGGGTCGGGGTGGGGGC11.0309278350515463No Hit
GGCGAGCGCACCGAGCTCTACGACGGCACCGTCACGGTGGACCACTGGTT11.0309278350515463No Hit
AGCTGCTGCGGGTGCCGGCGGACAACGGGCGGGGCATCCAGCCGCGCCCC11.0309278350515463No Hit
TAGGTGGCCTGACTGGCATTGTATTAGCAAACTCATCACTAGACATCGTA11.0309278350515463No Hit
ATCTTGGACAACCAGCTATCACCAGGCTCGGTAGGTTCTGTCTCTTATAC11.0309278350515463No Hit
AATATACTCTCCGGACAATGAACCATAACCAATACTACCAATCAATACTC11.0309278350515463No Hit
GGTCCACCCGGCGGAACTCGCGGTCCCGGCAGGCACCGCGGCGGCGGCAG11.0309278350515463No Hit
CGACGACGGCGTCGTGCTCGGCCGGGCCCGCTCGGAGCTGTCTCTTATAC11.0309278350515463No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT11.0309278350515463No Hit
GCGTACAGCTCCTGCAGCTGCAGCAGGTGGCCCTCGTCCACGATCGAGGC11.0309278350515463No Hit
ACCCTAGGCTCACTAAACATTCTACTACTCACTCTCACTGCCCAAGAACT11.0309278350515463No Hit
CCGCCGCGGCCGGCGCGGGAGCCTCCGAGCCGTGGGCCGCCGCCATCCGC11.0309278350515463No Hit
CCTCACGGGCGATCGTGACGCCGTCGTTGGTGATGGTGGGGGCGCCCCAC11.0309278350515463No Hit
CCGATGCACACCGCGATGAGGCGACGACCCTGAACCTGTCTCTTATACAC11.0309278350515463No Hit
CCTTAACAATGAACAAGAGATTCGAAAAATAGGAGGACTACTCAAAACCA11.0309278350515463No Hit
CTATTAAACCCATATAACCTCCCCCAAAATTCAGAATAATAACACACCCG11.0309278350515463No Hit
CCTACACCCCTTTTCCCCATACTAGTTATTATCGAAACCATCAGCCTACT11.0309278350515463No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG11.0309278350515463No Hit
CTCCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACCTGT11.0309278350515463No Hit
CTACTACTATCTCGCACCTGAAACAAGCTAACATGACTAACACCCTTAAT11.0309278350515463No Hit
ACTGCTTTGTGATGTGTGCATTCATCTCACAGAGGTAAACCTTTCTTTTA11.0309278350515463No Hit
TGTTAAAGCCACTTTCGTAGTCTATTTTGTGTCAACTGGAGTTTTTTACA11.0309278350515463No Hit
GCCTGGGACGGGGCGGAACCCGCTGCAGCCGCTCGCCGTAGCAGCGCGAG11.0309278350515463No Hit
CTTCGAGATCAACGAGGCCGCGCGACTGGTCCAGGAGGCTGTCTCTTATA11.0309278350515463No Hit
CCCAAGTGCCCCGGCGGACGGCCGCCTCCGGCTCGGGCTGCGCCGTCACG11.0309278350515463No Hit
CTTATACCATCTGACGCTGCCGGCGAACTTCTAGGTGAAGATCTCGGTGG11.0309278350515463No Hit
GGGCGATGGGGCGCCAATGAGGTCCCGGATCACCGGGCCACTGCCACAGC11.0309278350515463No Hit
CTTCCCCTACTCCTCGCACTGATTTCCACTGCCCACACTCTAGGCTCACT11.0309278350515463No Hit
CATGAATGCTGTGCAATGGTTTTTTTTTTTTTTTTTTTCCCTCCTTTAAA11.0309278350515463No Hit
CGCTGCCCCTGACCGTCGGCGTCGGCCAGGTCATCGCCGGCTGGGACCAG11.0309278350515463No Hit
GTCCAGCTCCACGACGCCGGAGACCTCGGCGCCCACCCGCGTCCAGTTCT11.0309278350515463No Hit
GCTTGTGCACTCTGTGCACCTGCAGAGTTAGCACTATGTTGATGCCACCA11.0309278350515463No Hit
AAGCTTATGTTGTTTATGCGGGGAAACGCCATATCGGGGGCACCGATTAT11.0309278350515463No Hit
GGCAAGCAGGGGGCCGCCCTGGCGCGCGCCGCCCTCGAGGCCGGCGCCTG11.0309278350515463No Hit
GAGGCGAAGCCCACGAGGTCGGCGGTCTGGCCGAGCAGGATCTCCAGGTC11.0309278350515463No Hit
GACCACACCGCTAACAATCAATACTAAACCCCCATAAATAGGAGAAGGCT11.0309278350515463No Hit
CTACTACGGCGGCCCCGGGTTCCTGGAAACGGCCAAGAACCCAGCCATCC11.0309278350515463No Hit
GGCTAAGGTTGTCTGGTAGTAAGGTGGAGTGGGTTTGGGGCCTGTCTCTT11.0309278350515463No Hit
GCCCAGTCGTCGGCGGACAGCTCGCCGGACAGGCGCTGTCTCTTATACAC11.0309278350515463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGA50.044.014
AACAATG50.044.05
CAATGAA50.044.07
AACAAGA50.044.012
TAGGAGG50.044.030
GAGGACT50.044.033
AGGACTA50.044.034
GGACTAC50.044.035
ATAGGAG50.044.029
TCGAAAA50.044.022
AAATAGG50.044.027
AAGAGAT50.044.015
GAAAAAT50.044.024
TTCGAAA50.044.021
ATGAACA50.044.09
ACTCAAA50.044.040
TCAAAAC50.044.042
AATAGGA50.044.028
ATTCGAA50.044.020
AGAGATT50.044.016