FastQCFastQC Report
Wed 25 May 2016
SRR1780625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCTGGTGGACCGGGACACGGACCTGCAGGCCTACCTGCTGTCTCTTAT11.0309278350515463No Hit
GCCCGGGAGACCGTGCTGGTGGACGTGGACTCCCGCCTGGCCAGCCTGGC11.0309278350515463No Hit
TTTGGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGG11.0309278350515463No Hit
GTGTGACCCTGGGAAGCATTCTCCGCCTCTGCCCGCCCCGCCTCCGAGCC11.0309278350515463No Hit
CCGCACAAGAGTGCTACTCTCCTCGCTCCGAGCCCACGAGACTTCGCGGC11.0309278350515463No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT11.0309278350515463No Hit
TCCTAGACCTAACCTGACTAGAAAAGCTATTACCTAAAACAATTTCACAG11.0309278350515463No Hit
GCCCGCGGCGCGCGGCGACGGGGCTGTCTCTTATACACATCTCCGAGCCC11.0309278350515463No Hit
CCTGTAATCCCAGCACTTTGGGATGCTGAGTCAGGCGGATCACCTGAGGT11.0309278350515463No Hit
ACTCTACCCAGCCCGGTTCCGGGCCGGGGCCGGACGAGCAGCACGCCCAG11.0309278350515463No Hit
GGCACCGGCTCCTCCGCCGCCGGCTGGCCCGACGCCCCGGCCGGCGACGC11.0309278350515463No Hit
ATGTGTAGGAAGAGGCAGATAAAGAATATTGAGGCGCCATTGGCGTGAAG11.0309278350515463No Hit
CCGTTCTCCGGGCGTTTTCTTTGGTCAGAGCGTCCACGCCAACGGAACCA11.0309278350515463No Hit
GCACCAGCTACACCACGTCCATGAACTTCAACGTGGGCACCGCCGTGCCT11.0309278350515463No Hit
GATCTCGAGGCGGCGGCGGCCGCCGACCTTCATGCCGAGCAGGCCCTGGT11.0309278350515463No Hit
ATCTCCGAGCCCACGAGCTAACAACAATCTCGTATGCCGTCTTCTGCTTG11.0309278350515463RNA PCR Primer, Index 36 (100% over 29bp)
ACCCTACAAACCTGCACACACAATACACATACTTACACACCTACACCCCT11.0309278350515463No Hit
GCTATTCCTAGTTTTATTGCTATAGCCATTATGATTATTAATGATGAGTA11.0309278350515463No Hit
GTCCTTGTAGTATAAACTAATACACCAGTCTTGTAAACCGAAGATGAAAA11.0309278350515463No Hit
GCCCAGCGCGTGGTCGATCGGCACCGAGAACCAGTGGTCCACCGTGACGG11.0309278350515463No Hit
CCTCTACACTTATCATCTTCACAATTCTAATCCTACTGACTATCCTAGAA11.0309278350515463No Hit
GCTGGACCCCGGCGGACCCGTCCCTCTCGACGTCGCTGCTCATCAAGGCG11.0309278350515463No Hit
GCGCCGGCCTCGAGGGCGGCGCGCGCCAGGGCGGCCCCCTGCTTGCCCTG11.0309278350515463No Hit
GAGACCTGTGGGAAGCGAAAACTGTCTCTTATACACATCTCCGAGCCCAC11.0309278350515463No Hit
TCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGAT11.0309278350515463No Hit
GCTCGGCGCCGAACAGGTCCTTGGCGCGCTGGATGGCCAGGTTCTCGGCC11.0309278350515463No Hit
ATCTCAGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTT11.0309278350515463TruSeq Adapter, Index 25 (96% over 28bp)
GTGCCCAGCGTCAACGTGCCGGGCGTCAGGGTGATCAGCGCCGAGAGCAG11.0309278350515463No Hit
GAATTAAGGGTGTTAGTCATGTTAGCTTGTTTCAGGTGCGAGATAGTAGT11.0309278350515463No Hit
CGATGAGACGGGGGTGGGCGCCCCGTCCGCGGCGACGGCCAGGTCCCCGT11.0309278350515463No Hit
CTTCTCGTCCCCATGGATGACCCCCCTCAGATAGGGGTCCCTTGACCACC11.0309278350515463No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT11.0309278350515463No Hit
CGTGCGAGCTGGAGCGTGGAGTGCATGAACGGCTCCACCACCTGTCTCTT11.0309278350515463No Hit
CGCCTGTCCGGCGAGCTGTCCGCCGACGACTGGGCCTGTCTCTTATACAC11.0309278350515463No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTCTGTCTCTTA11.0309278350515463No Hit
TCGCTGGGACGAGCGCTTTCATCTCCGAGCCCACAAGACACCGGCTAATC11.0309278350515463No Hit
TTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACAT11.0309278350515463No Hit
GAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCCCT11.0309278350515463No Hit
GCATGGAGGAGTCCGAGCTCGTGGCTGAGACCCTCGGCGAACACGTCCTG11.0309278350515463No Hit
CATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTAAAACTAGGCGGCT11.0309278350515463No Hit
CTCCAGGCCGGCATCGACAAGCTCGCCGACACCGTCAAGGTCACCCTCGG11.0309278350515463No Hit
CCTTCGACGAGCCGGCCCTGGGCTTTGCGCTGGCCGGCGGGCTGTCTCTT11.0309278350515463No Hit
TATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCACA11.0309278350515463No Hit
GTTGTAGCCCGTGCGAGAATAATGATGTATGCTTTGTTTCTGTTGAGTGT11.0309278350515463No Hit
GATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCA11.0309278350515463No Hit
TCCCTGAACCGAAGGAGCCCCCCATGACCGAGCCCACGAGACTGGGTTTC11.0309278350515463No Hit
GTTGTGGTAGTCAAAATGTAATAATTATTAGTAGTAAGGCTAGGAGCTGT11.0309278350515463No Hit
CTCCGAGCGGGCCCGGCCGAGCACGACGCCGTCGTCGCTGTCTCTTATAC11.0309278350515463No Hit
AGGCTCGACTGGATGCCCCGCCCCTTCTCCGCCGGCACCCACAGCAGCGC11.0309278350515463No Hit
CGCCAGGAACGTGGCCGCCGAGGCCGCACGCGGCGCTGTCTCTTATACAC11.0309278350515463No Hit
GGACCATTGCGGATAAGGAAATATCTTCACATAAGAAGTAGACAGAAGTA11.0309278350515463No Hit
ATACGGGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGAT11.0309278350515463No Hit
CCGTCGCCCTGGCGCACGCGCTGGACGGCGAGGTGGTCAACGGGGACGCC11.0309278350515463No Hit
TGTTTGTAGGGCTCATGGTAGGGGTAAAAGGAGGGCAATTTCTAGATCAA11.0309278350515463No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC11.0309278350515463No Hit
GTATTAGTTGATACTACAAGGACAGGCCCATTTGAGTATTTTGTTTTCAA11.0309278350515463No Hit
GCCGCGGTCCGGGCGCAGGCCCATGCGGGACATCATGTCTGTCTCTTATA11.0309278350515463No Hit
AATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATAATAA11.0309278350515463No Hit
GGCTGTTACTACGAGGGTTATGTGGCTGATTGAAGAGTATGCAATGAGCG11.0309278350515463No Hit
GCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCCTGTCTCTT11.0309278350515463No Hit
CCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATA11.0309278350515463No Hit
ACCCAGCCGTTCGAGAACTGGACGCGGGTGGGCGCCGAGGTCTCCGGCGT11.0309278350515463No Hit
GTTCGAAGGCGGCCCCGAGTCGGTCGACGGCAGCGCGGCCGGACGGAGGG11.0309278350515463No Hit
GGCCCTCCTCGGCGGCCCAGCGGGCGGTGGAGAGGGTGAGCATCTGTCTC11.0309278350515463No Hit
CTTATACACATCTCAGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT11.0309278350515463No Hit
CTCCAGAACCGTGACCAGCGGGTGAGGACTACAGCGGCGGCGGGAGTCTC11.0309278350515463No Hit
GTTATCCTTTAAAAGTTGAGAAAGCCATGTTGTTAGACATGGGGGCATGA11.0309278350515463No Hit
TTCCAGGCCTGGCGCGAGGCGAACCCGGAGAACGCGCGGCTGTACGACCG11.0309278350515463No Hit
ATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTGGTTTC11.0309278350515463No Hit
CGGTCGAGCGCCGCCCCGAGGGCAACGAGAACCCGAACCTTCCCTCCGGT11.0309278350515463No Hit
AGACCCAGCCCAGATGCAGGCCCTGCACCTGTCTCTTATACACATCTCCG11.0309278350515463No Hit
CGCCATCCTGCGCGGCCCGCGGGTGGACATCATGGCCGCCGCCACCGGCG11.0309278350515463No Hit
GTCCACGGCGCCGGGGAGCTCCTCGGCGTGCTCCCGGCCCGCGCGGCGGA11.0309278350515463No Hit
ATAATCCTCTCTCAAGGACTTCAAACTCTGCTCCCACTAATAGCTTTTTG11.0309278350515463No Hit
AAATAGGATTATCCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGT11.0309278350515463No Hit
AGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.0309278350515463Illumina PCR Primer Index 6 (96% over 29bp)
CCCTACTCGCGCTGCTACGGCGAGCGGCTGCAGCGGGTTCCGCCCCGTCC11.0309278350515463No Hit
CACGCCGGCTTCCCCGTGGTCATCTTCTTCGACGAGATGGAGTCGCTGTT11.0309278350515463No Hit
GCTAGGAGGAGGCCTAGTAGTGGGGTGAGGCTTGGATTAGCGTTTAGAAG11.0309278350515463No Hit
GTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGTTTGTGGGGT11.0309278350515463No Hit
GCCACGGGCGGCGACGACGACGGCGCCGCCCGACGGCGACGCTGTCTCTT11.0309278350515463No Hit
GTGCAGGATCGTCGCCTCATCGCGGGGTGCAGCGGCTATCTCTTATACAC11.0309278350515463No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG11.0309278350515463No Hit
GGCCCCGTCCGAGCCGCGTGACGGCGCAGCCCGAGCCGGAGGAGGCCGTC11.0309278350515463No Hit
GTCTCAATGAGCTCCAAATGGTTCATTCACAGAATGGACAAAAACAGTGT11.0309278350515463No Hit
CCTCCTGGACCAGTCGCGCGGCCTCGTTGATCTCGAAGCTGTCTCTTATA11.0309278350515463No Hit
GCGCTATCCGGTGCCGGCGTTGCCTGGCTCAACTTCCTGCCTGTCTCTTA11.0309278350515463No Hit
ACCGCGCACATCAGCGGGTTGCCCATGAACGTGGGCCCGTGCAGGAGAGC11.0309278350515463No Hit
GTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAA11.0309278350515463No Hit
CCCTACGACATGCTCGAGGTCATCGCCTCGATCGTGGACGAGGGCCACCT11.0309278350515463No Hit
GACCAACCGAACCCCCTTCGACCTTGCCGAAGGGGAGTCCGAACTAGTCT11.0309278350515463No Hit
AGTTTGTTGTTATGGAGAAGTATAGGTGAGGCCTAAGAAGTGGACAGTGA11.0309278350515463No Hit
CAAGCAGTGAGCCTTGGTGGCATCAACATAGTGCTAACTCTGCAGGTGCA11.0309278350515463No Hit
GCGCCACCATGCCGGAGCGCAGGACGCGGTCCACGGCCTCGCGCCTGTCT11.0309278350515463No Hit
AACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATCTGTCTCTTATAC11.0309278350515463No Hit
GAGCAAGATTCTTTAGATCGGGGAGGTAGGGCAAATGGGAAACTGGTTTC11.0309278350515463No Hit
GCAATAAGAAAGTGTTAAGGGAAAACCTGGTTAATTCTAAATTTTGATTC11.0309278350515463No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.044.01
GTATGCC50.044.026
AATCTCG50.044.020
ATCTCGT50.044.021
AAATCTC50.044.019
GCCCACG50.044.02
ATCCAAA50.044.014
TGCCGTC50.044.029
CCAAAAT50.044.016
CCACGAG50.044.04
TATGCCG50.044.027
GAAAAAA50.044.044
TTCTGCT50.044.036
CCGTCTT50.044.031
CACGAGA50.044.05
CCCACGA50.044.03
TCTGCTT50.044.037
TCTCGTA50.044.022
ACGAGAC50.044.06
TGCTTGA50.044.039