FastQCFastQC Report
Wed 25 May 2016
SRR1780624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167526
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA150.002154898870.539711
GAGGCAC207.913236E-569.992247
TTCGGAA352.9628063E-759.99334767
CAATACG308.354498E-658.32686269
AATTCAG252.3864312E-455.9937918
GTCGCCC452.687375E-854.43840837
CCGAAAT452.687375E-854.43840847
CTTCGGA407.451381E-752.4941866
CGGAATA407.451381E-752.4941869
TCCAGAT200.006944770552.4941847
GCTTCGG407.451381E-752.4941865
GTACGTA200.006944770552.4941813
TGAAGTT608.367351E-1152.49417539
GGTCACC608.367351E-1152.49417531
TTCGGTC608.367351E-1152.49417528
TTCTGAT608.367351E-1152.49417519
TCGTTGT352.0811378E-549.99445734
TCGCCCC506.164373E-848.99456438
TCGGTCA651.8371793E-1048.45616529
GTGACAC305.868158E-446.6614924
Produced by FastQC (version 0.11.5)