##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 167526 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30681207693134 34.0 31.0 34.0 12.0 34.0 2 31.320529350667957 34.0 32.0 34.0 23.0 34.0 3 31.568699783914138 34.0 33.0 34.0 23.0 34.0 4 32.008088296741995 34.0 34.0 34.0 27.0 34.0 5 31.986527464393586 34.0 34.0 34.0 27.0 34.0 6 34.27467378198011 38.0 34.0 38.0 24.0 38.0 7 33.67901698840777 37.0 34.0 38.0 21.0 38.0 8 32.25091627568258 37.0 31.0 38.0 11.0 38.0 9 33.267588314649664 37.0 34.0 38.0 21.0 38.0 10-11 33.81557489583706 37.0 34.0 38.0 22.5 38.0 12-13 34.297894058235734 37.0 34.5 38.0 24.5 38.0 14-15 33.88977830306938 37.0 34.0 38.0 22.5 38.0 16-17 33.77598402636009 37.0 34.0 38.0 22.0 38.0 18-19 33.72654990867089 37.0 34.0 38.0 22.0 38.0 20-21 33.786836073206544 37.0 34.0 38.0 22.0 38.0 22-23 33.85039337177513 37.0 34.0 38.0 22.0 38.0 24-25 34.334956961904425 37.5 34.5 38.0 24.5 38.0 26-27 34.436842639351504 38.0 34.5 38.0 25.0 38.0 28-29 34.598086267206284 38.0 35.5 38.0 26.0 38.0 30-31 34.435741317765604 38.0 35.0 38.0 24.5 38.0 32-33 34.155226651385455 37.0 34.0 38.0 23.5 38.0 34-35 33.9536579396631 37.0 34.0 38.0 23.0 38.0 36-37 33.95521889139596 37.0 34.0 38.0 23.5 38.0 38-39 34.593460119623224 37.5 35.0 38.0 26.0 38.0 40-41 33.97081945489059 37.5 34.0 38.0 23.0 38.0 42-43 34.38031410049783 37.0 34.5 38.0 24.5 38.0 44-45 33.26843594427134 37.0 34.0 38.0 21.5 38.0 46-47 34.102801953129664 37.0 34.0 38.0 24.0 38.0 48-49 33.78408127693612 37.0 34.0 38.0 23.0 38.0 50-51 33.74518582190227 37.0 34.0 38.0 23.0 38.0 52-53 33.771814524312646 37.0 34.0 38.0 23.0 38.0 54-55 33.42077050726454 37.0 34.0 38.0 21.5 38.0 56-57 33.596286546565906 37.0 34.0 38.0 22.5 38.0 58-59 33.44658142616669 37.0 33.0 38.0 22.5 38.0 60-61 33.45352960137531 37.0 33.5 38.0 22.0 38.0 62-63 33.55939675035516 37.0 33.5 38.0 22.5 38.0 64-65 33.54672110597758 37.0 33.5 38.0 22.5 38.0 66-67 33.47079856261118 37.0 33.5 38.0 22.5 38.0 68-69 33.03638539689362 37.0 32.0 38.0 21.0 38.0 70-71 32.79991165550422 37.0 31.5 38.0 20.0 38.0 72-73 32.94448622900326 37.0 32.0 38.0 20.5 38.0 74-75 32.47349366665473 37.0 31.0 38.0 19.5 38.0 76 31.484378544225972 36.0 28.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.594438251569162 1101 2 -0.7633377943655688 1101 3 -0.3952052125347336 1101 4 -2.6008238406375135 1101 5 -0.637188824233565 1101 6 -2.5736779069395297 1101 7 -1.2041215116584851 1101 8 -0.30346918480247 1101 9 -0.7258451286457728 1101 10-11 -1.1850973117430428 1101 12-13 -1.110938005871354 1101 14-15 -0.2540953256401437 1101 16-17 -1.9706363617861626 1101 18-19 -0.060586365698362954 1101 20-21 -1.3443230810394624 1101 22-23 -0.3966846577540011 1101 24-25 -1.123673848722838 1101 26-27 -1.1987383412406345 1101 28-29 -0.38384412629159925 1101 30-31 -1.1014364478586387 1101 32-33 0.23867671696022086 1101 34-35 -0.6481507330890821 1101 36-37 0.366445626385584 1101 38-39 -0.36312906534589473 1101 40-41 0.6666662267937369 1101 42-43 -0.4516411463332375 1101 44-45 0.4011947917541292 1101 46-47 0.6781400253714835 1101 48-49 -0.2411958333356523 1101 50-51 -0.7528395346398398 1101 52-53 -0.1583018127399356 1101 54-55 -1.1909504362969088 1101 56-57 -0.5890669911200987 1101 58-59 -0.37018221277075014 1101 60-61 0.24147205491450308 1101 62-63 -1.2029033425763984 1101 64-65 -0.21818615006186803 1101 66-67 -0.8888145720446445 1101 68-69 0.6486172576969906 1101 70-71 0.5367045457858168 1101 72-73 -0.06762896804865193 1101 74-75 -1.3527027497370838 1101 76 -1.0044449019054902 1102 1 0.3242715664541578 1102 2 -0.682592452750665 1102 3 0.48623752474324533 1102 4 0.41857247624164273 1102 5 0.8336622164115255 1102 6 1.1507418380751844 1102 7 2.1321539596284254 1102 8 2.812036971893619 1102 9 1.2405927050689485 1102 10-11 0.22832755477107014 1102 12-13 -3.6379076037791656 1102 14-15 -0.7388942795466491 1102 16-17 -2.0321488386304445 1102 18-19 -0.19853994529953667 1102 20-21 0.6363350861175547 1102 22-23 -0.3637218158666755 1102 24-25 0.6721744677204455 1102 26-27 -5.710615861127312 1102 28-29 0.15091668878957876 1102 30-31 0.7502035216303042 1102 32-33 -6.7241651703667955 1102 34-35 -0.1130630137908355 1102 36-37 0.9639720511431307 1102 38-39 1.0408700629095193 1102 40-41 1.0155819944738056 1102 42-43 0.9176516508771826 1102 44-45 -5.861690682801829 1102 46-47 0.22979089166765476 1102 48-49 0.7510129494909847 1102 50-51 -6.140929324331459 1102 52-53 -1.2152921145814872 1102 54-55 1.3335128959467397 1102 56-57 -0.6081364037821899 1102 58-59 1.1367372598237822 1102 60-61 0.8924473191620734 1102 62-63 -4.59328146178747 1102 64-65 -0.2805158874488214 1102 66-67 0.7943062735475976 1102 68-69 -7.815477762462102 1102 70-71 0.17667403472555776 1102 72-73 0.7070795423578033 1102 74-75 -6.422932672369754 1102 76 -1.8013284150361457 1103 1 -0.17118074070338807 1103 2 -0.3389857750768428 1103 3 -0.10704811091993705 1103 4 0.3619628729819766 1103 5 0.1979845522456074 1103 6 -0.8607722250131928 1103 7 0.8895620643701889 1103 8 1.23437859921453 1103 9 0.8506594238747667 1103 10-11 0.9626930502378599 1103 12-13 -1.068379076903362 1103 14-15 -0.24472855428137308 1103 16-17 -0.29123215506682953 1103 18-19 -0.358855773394815 1103 20-21 -0.1734052802082715 1103 22-23 -0.1618491512662672 1103 24-25 0.5995344885761043 1103 26-27 -0.41849659764113056 1103 28-29 0.556267228043609 1103 30-31 0.32933192877916184 1103 32-33 -4.320569499847625 1103 34-35 -0.3993166224961513 1103 36-37 0.25054670185907923 1103 38-39 0.712407878278988 1103 40-41 1.0824287343283956 1103 42-43 0.5888635965365907 1103 44-45 -5.0103403752773765 1103 46-47 0.3353050899316159 1103 48-49 0.43858114074512855 1103 50-51 0.5253004355387674 1103 52-53 0.05434213891135187 1103 54-55 0.3525919640076438 1103 56-57 1.165763122137733 1103 58-59 1.4127561457838667 1103 60-61 1.085671160272831 1103 62-63 0.54009338976077 1103 64-65 1.1993767445163428 1103 66-67 0.4353185455594968 1103 68-69 -2.5981468043568796 1103 70-71 0.8440552009046272 1103 72-73 1.1505906315833414 1103 74-75 -1.215143662144687 1103 76 0.7044793073518285 1104 1 -1.8534858706167796 1104 2 -2.9523688880317422E-5 1104 3 0.35717573984621964 1104 4 0.3864443297885529 1104 5 -0.22555975155185948 1104 6 -0.217587681375619 1104 7 1.795677987088382 1104 8 1.5701147750972808 1104 9 -0.0697047777685853 1104 10-11 0.0292886531692389 1104 12-13 -0.07845680286383327 1104 14-15 0.3854535465403046 1104 16-17 -0.4164508981270174 1104 18-19 0.9287369425723213 1104 20-21 1.4600879465294554 1104 22-23 0.45028276079235496 1104 24-25 0.8470028430064858 1104 26-27 0.8130912326941981 1104 28-29 -0.0798842265422266 1104 30-31 -0.36494521978845995 1104 32-33 -0.23611025045833145 1104 34-35 0.3406211967354764 1104 36-37 0.49852582688684066 1104 38-39 0.060229330644077095 1104 40-41 1.3118291340619095 1104 42-43 1.0710154952707711 1104 44-45 -2.910935534060407 1104 46-47 0.4868618298827627 1104 48-49 1.2602578007495708 1104 50-51 1.6604436733802146 1104 52-53 0.3989412950294877 1104 54-55 -0.2596221154949063 1104 56-57 0.9926873948448147 1104 58-59 1.5162338273295006 1104 60-61 0.535056014814252 1104 62-63 1.0409061812331188 1104 64-65 0.4576534489356163 1104 66-67 0.6639172575292918 1104 68-69 -3.330580737290475 1104 70-71 0.12056419490863135 1104 72-73 1.1073083753097421 1104 74-75 -0.19876791264434956 1104 76 1.0682368023551625 1105 1 -0.4462416731666323 1105 2 0.04411562178349726 1105 3 -0.45165067401451964 1105 4 0.12730081883726285 1105 5 0.21940919254751634 1105 6 0.4986768931040544 1105 7 1.8349980263168817 1105 8 1.4792484789220595 1105 9 1.3422599165759763 1105 10-11 1.5189998877774968 1105 12-13 -1.1524003574023496 1105 14-15 0.3329701641713356 1105 16-17 1.578726174990564 1105 18-19 1.342922406231466 1105 20-21 0.30627079495486953 1105 22-23 0.5376620786522253 1105 24-25 0.6946218906255339 1105 26-27 0.6022859586394915 1105 28-29 -0.39310641244176026 1105 30-31 0.3478933984963817 1105 32-33 1.3213834838775043 1105 34-35 1.023528464905901 1105 36-37 0.9692025186161644 1105 38-39 0.7381567579078876 1105 40-41 0.987577199878622 1105 42-43 1.1079099052086576 1105 44-45 2.0765244201730013 1105 46-47 1.1580899000582008 1105 48-49 1.0362368788796772 1105 50-51 0.38914586363846126 1105 52-53 -0.3500856200843856 1105 54-55 -0.13957199018162214 1105 56-57 0.35541377601513346 1105 58-59 0.7727294141225727 1105 60-61 0.9948228231394083 1105 62-63 1.2098132314674004 1105 64-65 0.8155939523681184 1105 66-67 1.0197067312135033 1105 68-69 1.8135404352059794 1105 70-71 1.8566348062195104 1105 72-73 0.8006286324743996 1105 74-75 -0.26481999938294365 1105 76 -0.5666111871839057 1106 1 -0.17194704597670452 1106 2 -0.11503542409010592 1106 3 -0.38771451269095536 1106 4 -0.6102420048822061 1106 5 0.08170482243917121 1106 6 -0.668741799855674 1106 7 1.0950737860027075 1106 8 1.1305868071954635 1106 9 1.4130896522526584 1106 10-11 0.15838471170121693 1106 12-13 0.18505983626763367 1106 14-15 -0.004643220039824314 1106 16-17 0.4652909003152814 1106 18-19 -0.25455784147299454 1106 20-21 -0.3840777367370052 1106 22-23 -0.08080527319395259 1106 24-25 0.4665095694638879 1106 26-27 -0.17885117938982376 1106 28-29 -0.8533418616614199 1106 30-31 -0.32563202649131284 1106 32-33 0.6574394309200002 1106 34-35 0.7819098443635184 1106 36-37 0.32650552950661194 1106 38-39 0.19217174272809956 1106 40-41 0.03416569294790861 1106 42-43 0.5515984549120247 1106 44-45 0.9827439561449225 1106 46-47 0.5220659837652235 1106 48-49 0.49508432895348164 1106 50-51 0.6409841501326241 1106 52-53 -0.5233905435355268 1106 54-55 0.10135263078784362 1106 56-57 0.8012052140565231 1106 58-59 -0.7100087971689604 1106 60-61 1.0502035248449317 1106 62-63 0.6257209086345839 1106 64-65 0.5769996844796381 1106 66-67 0.9871955204511664 1106 68-69 0.9553763077885904 1106 70-71 0.39771076087511403 1106 72-73 0.547796928531362 1106 74-75 -2.10818731316359 1106 76 -0.7483787275008709 1107 1 -0.36447810530185265 1107 2 0.015643470271431426 1107 3 0.009936740709029834 1107 4 0.2590974790551641 1107 5 -0.1761335220436635 1107 6 0.3486073143103141 1107 7 0.08765814239468028 1107 8 1.1680785369609552 1107 9 -1.0436478321257852 1107 10-11 0.019784770511776628 1107 12-13 0.17236502629923933 1107 14-15 -0.0711405000515839 1107 16-17 0.7606025962647109 1107 18-19 1.0868236552556638 1107 20-21 0.499197092344815 1107 22-23 -0.4022646939099701 1107 24-25 -0.15934961600991215 1107 26-27 0.24760805754320359 1107 28-29 -0.8021491100625013 1107 30-31 0.024120785388419108 1107 32-33 1.1869182223630688 1107 34-35 0.3539343977708498 1107 36-37 -0.3128525165126419 1107 38-39 0.7185813582540845 1107 40-41 0.8765120503690724 1107 42-43 0.6181876264183117 1107 44-45 0.6260165798308748 1107 46-47 0.7940188618068333 1107 48-49 0.48294546252695625 1107 50-51 1.2787892298944428 1107 52-53 1.2645560491278474 1107 54-55 0.8418878068519433 1107 56-57 1.1064320022650023 1107 58-59 1.023671273403707 1107 60-61 -0.08459670304597466 1107 62-63 -0.23469131660984743 1107 64-65 0.5416719994101697 1107 66-67 1.1539280426889107 1107 68-69 1.5305711177569776 1107 70-71 0.26267161509844783 1107 72-73 0.783117262281273 1107 74-75 -0.2051052724372795 1107 76 -0.6398089267216314 1108 1 -0.5812434999843177 1108 2 -0.08476636579414176 1108 3 -0.3423156356513495 1108 4 -0.07867287498809361 1108 5 0.3484738618935168 1108 6 0.5321381036752442 1108 7 1.1811799773581697 1108 8 1.5725075216695927 1108 9 -0.12695820545544478 1108 10-11 0.5598901569286383 1108 12-13 0.7276842851503247 1108 14-15 0.9047945460980245 1108 16-17 -0.9529082178970256 1108 18-19 0.20146582359841148 1108 20-21 0.3871379244153488 1108 22-23 -0.4075942847627019 1108 24-25 -1.0200139637162025 1108 26-27 -0.4153276085265034 1108 28-29 0.4221414258239804 1108 30-31 0.4731065272261361 1108 32-33 0.5097588340175676 1108 34-35 0.035433226810667406 1108 36-37 0.2391605018094367 1108 38-39 1.1261271634614118 1108 40-41 0.3375345741326683 1108 42-43 0.6624860835060673 1108 44-45 0.23952222799177747 1108 46-47 0.7963952221870514 1108 48-49 0.34108707982962017 1108 50-51 0.6448089984576058 1108 52-53 0.2704539000715087 1108 54-55 0.5824168053365781 1108 56-57 1.074540557162372 1108 58-59 -1.015177052812021 1108 60-61 1.2230989161853287 1108 62-63 0.4681861458505452 1108 64-65 0.6864836125800267 1108 66-67 0.4976479076840903 1108 68-69 1.4369115227714389 1108 70-71 2.025113067089073 1108 72-73 0.7585357083482194 1108 74-75 1.4824549975723684 1108 76 0.31702052637108125 1109 1 -0.20438905450242117 1109 2 -0.8244782999505205 1109 3 0.16677071493827 1109 4 -0.46613526735244903 1109 5 0.3416007422752969 1109 6 0.5448901180612467 1109 7 -0.6683376074535303 1109 8 0.5609139953701856 1109 9 1.041861174148245 1109 10-11 0.9536291340009484 1109 12-13 0.060338951955316134 1109 14-15 -0.10061749526173003 1109 16-17 -0.6723984831389274 1109 18-19 -0.028035853615683948 1109 20-21 0.8212683667157279 1109 22-23 0.9423728625900623 1109 24-25 0.8157271228418708 1109 26-27 0.6988915359214047 1109 28-29 0.1662193038887878 1109 30-31 0.6032419147144665 1109 32-33 1.011565729615306 1109 34-35 0.46415162775128493 1109 36-37 0.6013306785220962 1109 38-39 0.7444701796802491 1109 40-41 0.659758525441589 1109 42-43 0.6079290755176814 1109 44-45 0.10922257726729612 1109 46-47 -0.42730104644385847 1109 48-49 -0.045869554676031044 1109 50-51 1.4261573402634724 1109 52-53 0.6195997312434827 1109 54-55 0.3564597558501248 1109 56-57 0.5803070612948886 1109 58-59 0.5821445918952435 1109 60-61 0.5794852978985077 1109 62-63 -0.249756586798064 1109 64-65 -0.13770717484605655 1109 66-67 0.42197010569910276 1109 68-69 1.6963523361338275 1109 70-71 0.6611359947786681 1109 72-73 0.16559297569961018 1109 74-75 1.9779464040114831 1109 76 0.32468038043616687 1110 1 0.9983986794681101 1110 2 0.3727605159262879 1110 3 0.06379713203637039 1110 4 0.6186118453516514 1110 5 0.7671284204911473 1110 6 0.7013483684263022 1110 7 1.3655930974449149 1110 8 2.448847089754892 1110 9 -1.2664223782213284 1110 10-11 -0.32805147133557 1110 12-13 0.39738926391601836 1110 14-15 0.5541349274118375 1110 16-17 0.6900799451101136 1110 18-19 1.3541359238965995 1110 20-21 0.16490967827818537 1110 22-23 0.8653252056140914 1110 24-25 0.8340996172000317 1110 26-27 -0.8508363281433802 1110 28-29 0.5359392029993657 1110 30-31 0.368116466685791 1110 32-33 1.67391804608949 1110 34-35 1.2259862222023727 1110 36-37 0.506222487894199 1110 38-39 1.1114687194386477 1110 40-41 1.2938551668859049 1110 42-43 0.8228434522600239 1110 44-45 1.969324794179549 1110 46-47 0.8290576419936784 1110 48-49 1.160801094467729 1110 50-51 0.5774781951685526 1110 52-53 0.2679735530940093 1110 54-55 -0.07705278895274148 1110 56-57 0.8913517977876566 1110 58-59 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0.5679358193254131 2116 50-51 0.19598546904584424 2116 52-53 1.180117765617723 2116 54-55 0.23325714147103582 2116 56-57 -0.35932498905611254 2116 58-59 -0.4859333553330316 2116 60-61 -1.8659346972600517 2116 62-63 0.5861693391278564 2116 64-65 1.0337525201430537 2116 66-67 -0.46970503349237447 2116 68-69 0.35102916983026944 2116 70-71 -2.8094667648436946 2116 72-73 0.709165960449063 2116 74-75 -1.1954861788982214 2116 76 -1.268357624889422 2117 1 -0.7897023552245273 2117 2 -0.4550493846620647 2117 3 -0.07713409133550897 2117 4 -0.6461375967258469 2117 5 -1.9902767326839381 2117 6 -2.2514158839575487 2117 7 -3.1133935124150973 2117 8 -3.3263152149722366 2117 9 -0.3978080548291558 2117 10-11 -1.4810465007533011 2117 12-13 0.0031916579107402754 2117 14-15 -1.4259193432312927 2117 16-17 -0.882163410540123 2117 18-19 -0.9787307833462506 2117 20-21 -0.8773301269561458 2117 22-23 -0.7812663951162833 2117 24-25 -0.31408206265388827 2117 26-27 0.2578926229623235 2117 28-29 -0.5508152096603993 2117 30-31 -0.8638702446146738 2117 32-33 0.3742249034642171 2117 34-35 -0.6694185252108937 2117 36-37 -2.2282368344835675 2117 38-39 -0.5735814935863104 2117 40-41 -2.1882304488576594 2117 42-43 -1.8429939386914924 2117 44-45 -0.6137955141994382 2117 46-47 -2.0030736219543783 2117 48-49 -1.698967621296113 2117 50-51 0.1605553913543929 2117 52-53 0.3108752572936453 2117 54-55 -1.3948505166865672 2117 56-57 -1.6619526250161591 2117 58-59 -1.4954866294728788 2117 60-61 -0.15817387595040344 2117 62-63 -1.635151415589121 2117 64-65 -1.6262641280256531 2117 66-67 -2.89184987256008 2117 68-69 -1.0290290987601907 2117 70-71 0.0230915037467625 2117 72-73 0.2589717318142135 2117 74-75 -1.1194456683543805 2117 76 -1.395144528330043 2118 1 0.06264967372352359 2118 2 0.4049596616031188 2118 3 -0.1509473074981713 2118 4 -0.05729034937510846 2118 5 -0.5540529022367942 2118 6 0.046863171784679025 2118 7 0.25809567514413345 2118 8 -5.288654751458839 2118 9 -1.740144575894032 2118 10-11 -1.1932083427452014 2118 12-13 -0.15895187616352047 2118 14-15 -1.7369977872736797 2118 16-17 -1.0007287159592622 2118 18-19 -0.9772015337554834 2118 20-21 -3.8782778309278854 2118 22-23 -0.8010174074364329 2118 24-25 -0.7822209012857542 2118 26-27 0.46814290296577354 2118 28-29 -0.7286873988565787 2118 30-31 -0.7091629559095836 2118 32-33 -0.4980881403886386 2118 34-35 0.8142981974564591 2118 36-37 -0.7972684533342616 2118 38-39 -0.5598451706777183 2118 40-41 -1.4546644972767169 2118 42-43 -0.8672950070123377 2118 44-45 0.006045960772567582 2118 46-47 -0.9880288214029846 2118 48-49 -1.83920627038718 2118 50-51 -0.6321182107822523 2118 52-53 -1.4414240017741662 2118 54-55 0.09428319572835875 2118 56-57 0.6682629776374114 2118 58-59 0.20963204891085496 2118 60-61 -2.6311804668007497 2118 62-63 -0.17885349192236788 2118 64-65 -1.0164489303001432 2118 66-67 -2.7245855062294204 2118 68-69 0.8941368786672257 2118 70-71 -1.5356338344112466 2118 72-73 -2.58656329015529 2118 74-75 -0.1592653892124858 2118 76 0.06919810973319329 2119 1 -0.31045242493360803 2119 2 0.3918346194275344 2119 3 -0.07116234674958122 2119 4 -0.5856998236986151 2119 5 -0.2121607367735372 2119 6 0.14449684027333376 2119 7 -3.005867255328006 2119 8 -0.5741890880087617 2119 9 -0.9430260118883638 2119 10-11 -0.34158767558368197 2119 12-13 -0.03879644507279778 2119 14-15 -1.3491173634933986 2119 16-17 -0.5669331382099756 2119 18-19 -1.6451728318637677 2119 20-21 -2.0609206093429826 2119 22-23 0.21903567756705655 2119 24-25 -0.9355094722644495 2119 26-27 -0.2517156120037569 2119 28-29 0.586296743123313 2119 30-31 -0.33778162113666355 2119 32-33 -0.4708989463359572 2119 34-35 0.06375834996337204 2119 36-37 -1.322669402566106 2119 38-39 -0.733732297564373 2119 40-41 -0.9215932149139832 2119 42-43 -1.3323342584238134 2119 44-45 -2.086144245494168 2119 46-47 -0.7072631377086296 2119 48-49 -1.2908674261106938 2119 50-51 0.2158753560351272 2119 52-53 -2.0763296409310996 2119 54-55 -2.455253435173411 2119 56-57 -0.07599551933719084 2119 58-59 0.617610544985709 2119 60-61 0.1284524369039559 2119 62-63 -0.8657025820544035 2119 64-65 0.03105764909118136 2119 66-67 0.5886722484548841 2119 68-69 -1.1458905635803376 2119 70-71 -1.1503795437483575 2119 72-73 -2.6572495712808717 2119 74-75 -0.5388793982549274 2119 76 -0.6511046547358426 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 23.0 13 78.0 14 231.0 15 456.0 16 737.0 17 964.0 18 1058.0 19 1039.0 20 1155.0 21 1188.0 22 1379.0 23 1601.0 24 1962.0 25 2289.0 26 2681.0 27 3308.0 28 3925.0 29 4661.0 30 5626.0 31 6925.0 32 8797.0 33 11395.0 34 15261.0 35 22455.0 36 34332.0 37 33998.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.50613423141689 21.5155159971133 11.193168150108251 28.78518162136156 2 18.20493535331829 18.9272112985447 36.07320654704345 26.794646801093563 3 17.624726908062033 23.338466864844857 29.65569523536645 29.381110991726654 4 13.156166803958788 18.30223368312978 37.85860105297088 30.682998459940546 5 12.799207287227057 36.7405656435419 36.284517030192326 14.175710039038716 6 28.54601673770042 37.22646037033058 17.289256593006456 16.93826629896255 7 28.197414132731634 30.16904838651911 22.128505426023423 19.505032054725834 8 23.943746045389968 33.44376395305803 18.859162159903537 23.75332784164846 9 23.550971192531307 15.871566204648829 21.588887695044352 38.98857490777551 10-11 24.8895693802753 25.2575719589795 27.03699724221912 22.81586141852608 12-13 24.287573272208494 23.62379570932273 26.286367489225555 25.802263529243223 14-15 21.706182920860044 24.94448622900326 24.027912085288254 29.32141876484844 16-17 22.06403782099495 27.854482289316284 26.440970356840133 23.640509532848633 18-19 21.72946288934255 27.13698172224013 26.887766675023578 24.245788713393743 20-21 21.09582990103029 27.600193402815087 26.760025309504197 24.54395138665043 22-23 22.59500017907668 26.814345235963373 27.345606055179495 23.24504852978045 24-25 22.084034716999152 27.47752587658035 26.847175960746387 23.591263445674105 26-27 20.852584076501557 27.943722168499217 27.826426942683526 23.3772668123157 28-29 21.894213435526424 27.047443381922804 28.72688418514141 22.331458997409356 30-31 21.858099638265106 26.9940188388668 27.98311903823884 23.16476248462925 32-33 20.84243639793226 28.18696799302795 27.209806239031554 23.760789370008236 34-35 22.08642240607428 27.251889258980693 27.91566682186646 22.746021513078567 36-37 22.466064969019733 26.992526533194848 26.99909267815145 23.542315819633966 38-39 21.62619533684324 27.728233229468856 28.019232835500162 22.626338598187743 40-41 21.960471807361245 26.18548762580137 28.19054952664064 23.663491040196746 42-43 21.487112448216994 26.33024127598104 28.377087735635065 23.8055585401669 44-45 20.9749531416019 25.762269737234817 28.510798323842266 24.751978797321012 46-47 20.986294664708762 25.409488676384562 29.63629526163103 23.967921397275646 48-49 20.088822033594784 26.00282941155403 30.01772859138283 23.890619963468357 50-51 20.041665174360997 26.18309993672624 29.354547950765852 24.420686938146915 52-53 19.275813903513484 26.54334252593627 29.513030813127518 24.667812757422727 54-55 18.79051609899359 26.916717405059515 29.173978964459245 25.11878753148765 56-57 18.72724233850268 26.959397347277438 29.26321884364218 25.050141470577703 58-59 19.23223857789239 26.5391640700548 28.575862851139522 25.652734500913294 60-61 19.361173787949333 26.091173907333786 28.830151737640726 25.717500567076158 62-63 19.363859938158853 26.106992347456515 28.805677924620653 25.723469789763975 64-65 20.452049234148728 26.03655551974022 27.68256867590702 25.82882657020403 66-67 20.400713919033464 25.709442116447594 27.22562467915428 26.66421928536466 68-69 20.12881582560319 25.39993792008405 27.1647386077385 27.306507646574264 70-71 21.137315998710648 25.455153229946397 26.72510535678044 26.682425414562516 72-73 20.897651707794612 25.380239485214233 26.439478051168173 27.28263075582298 74-75 21.28744194930936 25.30323651254134 26.379785824289957 27.029535713859342 76 22.065231665532515 23.767653976099233 27.14623401740625 27.020880340962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 2.5 14 4.0 15 3.0 16 4.0 17 6.0 18 15.5 19 22.0 20 22.0 21 25.0 22 42.5 23 77.0 24 108.0 25 122.0 26 204.0 27 404.0 28 590.0 29 658.0 30 856.0 31 1255.0 32 1845.5 33 2235.0 34 2620.0 35 3487.5 36 4402.5 37 4835.0 38 5494.0 39 6896.0 40 8692.5 41 10312.5 42 10879.0 43 11119.0 44 11840.5 45 12481.5 46 12641.0 47 12703.5 48 12299.0 49 11341.0 50 10850.0 51 10274.5 52 8810.5 53 7109.5 54 6297.0 55 5819.0 56 4672.5 57 3475.0 58 2946.0 59 2463.5 60 1720.5 61 1234.0 62 1008.0 63 868.5 64 603.5 65 415.0 66 293.5 67 235.0 68 190.5 69 128.5 70 92.0 71 73.0 72 54.0 73 30.5 74 25.5 75 25.0 76 21.0 77 14.0 78 10.5 79 10.0 80 7.0 81 3.5 82 4.5 83 6.0 84 5.5 85 5.0 86 3.5 87 2.0 88 2.0 89 1.5 90 1.0 91 2.0 92 3.0 93 2.0 94 1.5 95 2.0 96 2.0 97 1.5 98 0.5 99 5.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7437651469025703 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 167526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.29324403376192 #Duplication Level Percentage of deduplicated Percentage of total 1 77.204858378038 38.05677924620656 2 8.410128483028373 8.291250313384191 3 4.319500115041355 6.387665198237885 4 2.386805362138074 4.706135167078543 5 1.5488199178968018 3.817317908861908 6 1.0874435389142518 3.216217184198274 7 0.7834921711330968 2.703460955314399 8 0.6175904285593189 2.4354428566312096 9 0.502549074219838 2.2295046739013644 >10 3.0867411811719685 26.369041223451884 >50 0.04843846498504462 1.5830378568102863 >100 0.0036328848738783467 0.20414741592349844 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002387689075128637 0.0 2 0.0 0.0 0.0 0.002984611343910796 0.0 3 0.0 0.0 0.0 0.002984611343910796 0.0 4 0.0 0.0 0.0 0.002984611343910796 0.0 5 0.0 0.0 0.0 0.002984611343910796 0.0 6 0.0 0.0 0.0 0.002984611343910796 0.0 7 0.0 0.0 0.0 0.004775378150257274 0.0 8 0.0 0.0 0.0 0.004775378150257274 0.0 9 0.0 0.0 0.0 0.011938445375643184 0.0 10 0.0 0.0 0.0 0.0161169012571183 0.0 11 0.0 0.0 0.0 0.017907668063464777 0.0 12 0.0 0.0 0.0 0.018504590332246936 0.0 13 0.0 0.0 0.0 0.019101512601029096 0.0 14 0.0 0.0 0.0 0.02268304621372205 0.0 15 0.0 0.0 0.0 0.02268304621372205 0.0 16 0.0 0.0 0.0 0.024473813020068527 0.0 17 0.0 0.0 0.0 0.02805534663276148 0.0 18 0.0 0.0 0.0 0.0322338025142366 0.0 19 0.0 0.0 0.0 0.0393968697396225 0.0 20 0.0 0.0 0.0 0.04297840335231546 0.0 21 0.0 0.0 0.0 0.04476917015866194 0.0 22 0.0 0.0 0.0 0.04715685923379057 0.0 23 0.0 0.0 0.0 0.04954454830891921 0.0 24 0.0 0.0 0.0 0.05312608192161217 0.0 25 0.0 0.0 0.0 0.05611069326552296 0.0 26 0.0 0.0 0.0 0.06506452729725536 0.0 27 0.0 0.0 0.0 0.093716796198799 0.0 28 0.0 0.0 0.0 0.119981376025214 0.0 29 0.0 0.0 0.0 0.15997516803361866 0.0 30 0.0 0.0 0.0 0.19399973735420173 0.0 31 0.0 0.0 0.0 0.22265200625574538 0.0 32 0.0 0.0 0.0 0.2751811659085754 0.0 33 0.0 0.0 0.0 0.3133841911106336 0.0 34 0.0 0.0 0.0 0.3641225839571171 0.0 35 0.0 0.0 0.0 0.43694710074854054 0.0 36 0.0 0.0 0.0 0.5831930566001695 0.0 37 0.0 0.0 0.0 0.854792688896052 0.0 38 0.0 0.0 0.0 1.2284660291536835 0.0 39 0.0 0.0 0.0 1.7877821950025667 0.0 40 0.0 0.0 0.0 2.462304358726407 0.0 41 0.0 0.0 0.0 3.3009801463653403 0.0 42 0.0 0.0 0.0 4.275754211286606 0.0 43 0.0 0.0 0.0 5.317980492580256 0.0 44 0.0 0.0 0.0 6.243806931461385 0.0 45 0.0 0.0 0.0 7.155307235891742 0.0 46 0.0 0.0 0.0 8.056659861752802 0.0 47 0.0 0.0 0.0 8.970547855258289 0.0 48 0.0 0.0 0.0 9.782362140802025 0.0 49 0.0 0.0 0.0 10.536274966273892 0.0 50 0.0 0.0 0.0 11.275264735026205 0.0 51 0.0 0.0 0.0 12.094242087795328 0.0 52 0.0 0.0 0.0 13.104831488843523 0.0 53 0.0 0.0 0.0 14.062294807970106 0.0 54 0.0 0.0 0.0 14.994687391807838 0.0 55 0.0 0.0 0.0 15.827990879027732 0.0 56 0.0 0.0 0.0 16.5049007318267 0.0 57 0.0 0.0 0.0 17.20628439764574 0.0 58 0.0 0.0 0.0 17.871852727337846 0.0 59 0.0 0.0 0.0 18.55294103601829 0.0 60 0.0 0.0 0.0 19.197617086303023 0.0 61 0.0 0.0 0.0 19.887062306746415 0.0 62 0.0 0.0 0.0 20.572925993577115 0.0 63 0.0 0.0 0.0 21.30654346191039 0.0 64 0.0 0.0 0.0 22.12134235879804 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCCTA 15 0.0021548988 70.53971 1 GAGGCAC 20 7.913236E-5 69.99224 7 TTCGGAA 35 2.9628063E-7 59.993347 67 CAATACG 30 8.354498E-6 58.326862 69 AATTCAG 25 2.3864312E-4 55.99379 18 GTCGCCC 45 2.687375E-8 54.438408 37 CCGAAAT 45 2.687375E-8 54.438408 47 CTTCGGA 40 7.451381E-7 52.49418 66 CGGAATA 40 7.451381E-7 52.49418 69 TCCAGAT 20 0.0069447705 52.49418 47 GCTTCGG 40 7.451381E-7 52.49418 65 GTACGTA 20 0.0069447705 52.49418 13 TGAAGTT 60 8.367351E-11 52.494175 39 GGTCACC 60 8.367351E-11 52.494175 31 TTCGGTC 60 8.367351E-11 52.494175 28 TTCTGAT 60 8.367351E-11 52.494175 19 TCGTTGT 35 2.0811378E-5 49.994457 34 TCGCCCC 50 6.164373E-8 48.994564 38 TCGGTCA 65 1.8371793E-10 48.456165 29 GTGACAC 30 5.868158E-4 46.66149 24 >>END_MODULE