FastQCFastQC Report
Wed 25 May 2016
SRR1780624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167526
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT10150.6058761028138916No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT3920.2339935293626064RNA PCR Primer, Index 24 (95% over 23bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC2640.15758747895849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGA252.830404E-670.0000160
ACGTCTC252.830404E-670.0000158
CTCGAGT207.908891E-570.062
CGTCTCG308.349009E-658.33333259
TCTCGAG308.349009E-658.33333261
CGAGTGA252.385124E-456.00000464
CTCGAAC252.385124E-456.00000433
GAGGCGC452.6850103E-854.44444713
CGAACTG200.006941725552.535
TGAAGGT407.4456693E-752.528
TCTGTCA200.006941725552.516
GTACTCG200.006941725552.53
TACTCGT200.006941725552.54
CGTCCAA200.006941725552.517
CGTGCGA352.0797732E-550.010
CGAGAAT352.0797732E-550.014
TTCTGTT352.0797732E-550.038
GCGTGAA506.158916E-849.00000425
CGCCATT506.158916E-849.00000417
TGGCGTG451.6771173E-646.66666823
Produced by FastQC (version 0.11.5)