##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780624_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 167526 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82401537671765 34.0 34.0 34.0 31.0 34.0 2 33.3066867232549 34.0 34.0 34.0 32.0 34.0 3 33.50798085073362 34.0 34.0 34.0 34.0 34.0 4 33.47757363036185 34.0 34.0 34.0 34.0 34.0 5 33.5037247949572 34.0 34.0 34.0 34.0 34.0 6 37.22513520289388 38.0 37.0 38.0 36.0 38.0 7 36.82048159688645 38.0 37.0 38.0 34.0 38.0 8 36.90524455905352 38.0 37.0 38.0 35.0 38.0 9 36.71880782684479 38.0 37.0 38.0 34.0 38.0 10-11 36.44645308787889 38.0 37.0 38.0 34.0 38.0 12-13 36.32871912419564 38.0 37.0 38.0 34.0 38.0 14-15 36.58828778816422 38.0 37.0 38.0 34.0 38.0 16-17 36.73691247925695 38.0 37.0 38.0 34.5 38.0 18-19 36.75158482862362 38.0 37.0 38.0 34.0 38.0 20-21 36.48000907321848 38.0 37.0 38.0 34.0 38.0 22-23 36.551735253035346 38.0 37.0 38.0 34.0 38.0 24-25 35.90965879923117 38.0 36.5 38.0 32.5 38.0 26-27 36.12107374377709 38.0 37.0 38.0 33.5 38.0 28-29 35.99792271050464 38.0 37.0 38.0 33.0 38.0 30-31 35.746913911870394 38.0 36.5 38.0 31.5 38.0 32-33 36.230015042441174 38.0 37.0 38.0 34.0 38.0 34-35 36.299371440850976 38.0 37.0 38.0 34.0 38.0 36-37 36.4265248379356 38.0 37.0 38.0 34.0 38.0 38-39 36.129075486790114 38.0 37.0 38.0 32.5 38.0 40-41 35.613480892518176 38.0 36.0 38.0 31.0 38.0 42-43 35.973675727946706 38.0 36.5 38.0 32.5 38.0 44-45 36.15020951971634 38.0 37.0 38.0 34.0 38.0 46-47 36.45505772238339 38.0 37.0 38.0 34.0 38.0 48-49 36.261971276100425 38.0 37.0 38.0 34.0 38.0 50-51 36.403223977173695 38.0 37.0 38.0 34.0 38.0 52-53 36.33630003700918 38.0 37.0 38.0 34.0 38.0 54-55 36.50972983298115 38.0 37.0 38.0 34.0 38.0 56-57 36.56559877272782 38.0 37.0 38.0 34.0 38.0 58-59 36.62523130737915 38.0 37.0 38.0 34.0 38.0 60-61 36.30887742798133 38.0 37.0 38.0 34.0 38.0 62-63 36.55968625765553 38.0 37.0 38.0 34.0 38.0 64-65 36.56009514940965 38.0 37.0 38.0 34.0 38.0 66-67 36.01371727373662 38.0 37.0 38.0 33.0 38.0 68-69 35.55684789226747 38.0 36.0 38.0 31.0 38.0 70-71 35.62851437985745 38.0 36.0 38.0 31.0 38.0 72-73 35.4076561250194 38.0 36.0 38.0 29.0 38.0 74-75 36.01868665162422 38.0 37.0 38.0 33.0 38.0 76 35.42292539665485 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33041396755714203 1101 2 0.5280203630855667 1101 3 0.20802645256654273 1101 4 0.2924537549500883 1101 5 0.1462382488185625 1101 6 -0.9529654134323167 1101 7 0.0709584303241968 1101 8 0.4532554721193307 1101 9 0.6059460329251181 1101 10-11 0.23198540133176238 1101 12-13 0.7649724305393235 1101 14-15 0.713873563919968 1101 16-17 -0.007417737108269762 1101 18-19 -0.4655251185771192 1101 20-21 0.06220232766985134 1101 22-23 0.623938060815469 1101 24-25 -1.3291122270731606 1101 26-27 -0.29606315681380835 1101 28-29 0.5884502556115905 1101 30-31 1.0587866660009837 1101 32-33 0.03461402270514924 1101 34-35 0.290752103433924 1101 36-37 0.20054733777414668 1101 38-39 0.2637274651074222 1101 40-41 1.2619109289192423 1101 42-43 0.5428669577026923 1101 44-45 0.4911543961783664 1101 46-47 0.6156522329743268 1101 48-49 -1.3324944310278681 1101 50-51 -1.206934168042821 1101 52-53 -1.2420629948810813 1101 54-55 -0.6934125121623822 1101 56-57 -0.645076446801589 1101 58-59 -0.6155954311362635 1101 60-61 -1.423653338659392 1101 62-63 -0.537620046944312 1101 64-65 -0.9737076047903983 1101 66-67 -1.3763959858688963 1101 68-69 -1.1229021182334264 1101 70-71 -0.4678266524101957 1101 72-73 -1.5201544693474887 1101 74-75 -0.7158668794470344 1101 76 -0.7884514695876774 1102 1 -0.54383687694542 1102 2 0.11786453874209712 1102 3 0.32320570504556656 1102 4 0.2875501916941232 1102 5 0.15299426930455695 1102 6 0.3032185246738237 1102 7 0.23571815572465482 1102 8 0.5097009335991132 1102 9 0.6886527998424157 1102 10-11 0.6461730443523521 1102 12-13 0.6916369178445407 1102 14-15 -0.04645116093565349 1102 16-17 0.308698640792997 1102 18-19 -0.03995031199544741 1102 20-21 0.5409535535606693 1102 22-23 0.36660995794960627 1102 24-25 -4.8803272210799165 1102 26-27 -1.203276843203696 1102 28-29 0.03129650166149389 1102 30-31 1.3842198140885955 1102 32-33 0.5124390199809454 1102 34-35 0.7887906781315408 1102 36-37 -2.426182530374483 1102 38-39 -0.034627117000020746 1102 40-41 0.9420896365579026 1102 42-43 0.9200054561226594 1102 44-45 0.87352336207136 1102 46-47 1.0258080573177963 1102 48-49 -3.2628093487569743 1102 50-51 -0.4383823537244069 1102 52-53 0.03466142486394119 1102 54-55 0.4540212897336602 1102 56-57 0.7719988501364128 1102 58-59 0.2113632699643162 1102 60-61 -5.637503180655685 1102 62-63 -2.4953404348706485 1102 64-65 -0.272280123042556 1102 66-67 0.8800058883819517 1102 68-69 -0.8753811418794939 1102 70-71 -1.469134269278456 1102 72-73 -6.416035476212471 1102 74-75 -2.1938008447951844 1102 76 -0.044308503476749195 1103 1 0.45929629997045396 1103 2 0.45702007756163 1103 3 0.023149703732435967 1103 4 0.28503013260307597 1103 5 0.22548700361694785 1103 6 0.271075702014528 1103 7 0.8091329806239997 1103 8 0.4610280681664065 1103 9 0.18099124088171692 1103 10-11 0.11628475731665588 1103 12-13 -0.7181261386685023 1103 14-15 -0.2106291485574303 1103 16-17 -0.12868609788363017 1103 18-19 0.4864909976628482 1103 20-21 -0.524319356286874 1103 22-23 0.4124996546735247 1103 24-25 -3.091135957284763 1103 26-27 -0.6074904592471029 1103 28-29 -0.0779784744413945 1103 30-31 0.6330466831324202 1103 32-33 0.7453465502264862 1103 34-35 0.025690715787597185 1103 36-37 -1.058200798389045 1103 38-39 -0.1979964649709416 1103 40-41 -0.4386854988590656 1103 42-43 -0.6615126622332212 1103 44-45 0.8483686343053307 1103 46-47 0.7823109684205392 1103 48-49 -2.099736904299654 1103 50-51 -0.6015996291639709 1103 52-53 -0.06067598304770172 1103 54-55 -0.41558979080202363 1103 56-57 0.14701454015948912 1103 58-59 -0.5030831377445537 1103 60-61 -4.31819190025325 1103 62-63 -1.2295635132048943 1103 64-65 -0.10421583739724127 1103 66-67 -0.8000262640961111 1103 68-69 0.11921424863311358 1103 70-71 0.4319703083461235 1103 72-73 -5.002499572611974 1103 74-75 -2.3791627774198147 1103 76 -3.482528434150982 1104 1 -0.06136548106441353 1104 2 0.06807048839884544 1104 3 0.18627206660163154 1104 4 -0.34393722249352265 1104 5 -0.3234359366450974 1104 6 0.10578145373560943 1104 7 0.32359000927156956 1104 8 0.5402730159789755 1104 9 0.8781264840529417 1104 10-11 -0.5016988091945578 1104 12-13 0.2616140345493463 1104 14-15 0.6275076491330012 1104 16-17 0.36371008244059766 1104 18-19 0.6070062097436804 1104 20-21 -0.07900067984893866 1104 22-23 0.16834908838438878 1104 24-25 0.6829679734280987 1104 26-27 1.011505765491954 1104 28-29 1.342234716764466 1104 30-31 0.7628468163769284 1104 32-33 0.495917280850513 1104 34-35 0.42714308087753494 1104 36-37 1.0263117488017173 1104 38-39 1.1631760866613092 1104 40-41 1.5460713299217446 1104 42-43 0.41785442637436887 1104 44-45 0.7410290828951602 1104 46-47 0.9724442129242021 1104 48-49 0.22098927824531245 1104 50-51 0.4587801176714663 1104 52-53 0.5386888848181712 1104 54-55 0.06207620964212879 1104 56-57 -0.1450263214883023 1104 58-59 -0.5266230000585637 1104 60-61 0.7154945310147909 1104 62-63 0.3275428603238595 1104 64-65 0.5663926458361672 1104 66-67 0.4616992522263459 1104 68-69 1.6083259519420139 1104 70-71 1.544504174657476 1104 72-73 1.3503718464419876 1104 74-75 0.23736620325973234 1104 76 0.06287685121432673 1105 1 0.7225900599620658 1105 2 0.26704182979581503 1105 3 0.16879794651881497 1105 4 -0.018976995839935284 1105 5 -0.2644624158866762 1105 6 0.21800549209236664 1105 7 0.7994099940160382 1105 8 -0.39451613080101566 1105 9 0.9144346457615598 1105 10-11 0.6299912856076659 1105 12-13 -0.11276539227858962 1105 14-15 0.06169965087648421 1105 16-17 0.38786394535875957 1105 18-19 0.6121691170118524 1105 20-21 0.4989463616678904 1105 22-23 0.5320779758203713 1105 24-25 0.7728274046147519 1105 26-27 0.7490496251410761 1105 28-29 0.8224591909935199 1105 30-31 1.3357290218907139 1105 32-33 0.8482241349422637 1105 34-35 -0.7482039824329192 1105 36-37 0.5862943139101375 1105 38-39 0.37138792059396764 1105 40-41 0.6303319815508175 1105 42-43 0.4809186085689916 1105 44-45 0.8455185674761765 1105 46-47 0.959991341615499 1105 48-49 -0.26515216231260297 1105 50-51 0.6605606559737538 1105 52-53 0.4305969157651006 1105 54-55 0.76872108269432 1105 56-57 0.5849551539393971 1105 58-59 0.41175555502479 1105 60-61 0.7988049810529319 1105 62-63 -0.13716238104042588 1105 64-65 0.5134886467079056 1105 66-67 0.6966126226088178 1105 68-69 0.6781221816467138 1105 70-71 1.1350392078927314 1105 72-73 1.2335776871306692 1105 74-75 0.034214846607234506 1105 76 0.6719386361113564 1106 1 -1.1779196467476787 1106 2 0.12076343151898072 1106 3 0.30223619216216946 1106 4 0.20715081149278802 1106 5 -0.01963607825472735 1106 6 0.12626292647575355 1106 7 -1.1668277391353215 1106 8 0.2391495795853018 1106 9 -0.5228175801434247 1106 10-11 0.46758842233718667 1106 12-13 1.1281870815165433 1106 14-15 -0.39946190479666654 1106 16-17 0.24492556509341767 1106 18-19 0.5507234625168707 1106 20-21 0.2621416378286554 1106 22-23 0.4499492771972342 1106 24-25 -0.30208277002245865 1106 26-27 0.9134741393413819 1106 28-29 0.8270990075362405 1106 30-31 0.5650158038680786 1106 32-33 0.13191444865792334 1106 34-35 0.939779380145886 1106 36-37 0.9753936463707547 1106 38-39 0.14371892357421956 1106 40-41 0.7983866473633299 1106 42-43 0.6757468030559863 1106 44-45 1.1625301449673842 1106 46-47 0.7424630942081265 1106 48-49 1.0871280556821787 1106 50-51 -0.016511362149159936 1106 52-53 0.4044523967369855 1106 54-55 0.24481871613257056 1106 56-57 0.37379359826623926 1106 58-59 0.6361100657899073 1106 60-61 0.8622351493768434 1106 62-63 0.7053809533141759 1106 64-65 -0.6526302477206016 1106 66-67 0.25501999803705644 1106 68-69 1.4596051589280918 1106 70-71 0.3732249676714048 1106 72-73 0.15402537488192536 1106 74-75 -0.4550269994780507 1106 76 0.06031843724218078 1107 1 0.8170814559502517 1107 2 -0.15469051246396504 1107 3 0.19889501742130733 1107 4 0.13414301909405424 1107 5 0.09774611684929368 1107 6 0.21737334329557 1107 7 0.7295002767435292 1107 8 -0.11688401594509656 1107 9 -0.5547682527891666 1107 10-11 0.30052509071915523 1107 12-13 -0.2411340241219193 1107 14-15 0.2199235248879603 1107 16-17 0.6191307036657889 1107 18-19 0.5416675557315997 1107 20-21 0.6711093287791883 1107 22-23 0.08134951979077698 1107 24-25 0.17482949197857067 1107 26-27 -0.26870993770044294 1107 28-29 0.7425496434253844 1107 30-31 0.2809378431241214 1107 32-33 0.752099334344905 1107 34-35 -0.060050620589244375 1107 36-37 1.0360787893539154 1107 38-39 0.15712129805043418 1107 40-41 0.40482456546963164 1107 42-43 0.718182295226832 1107 44-45 0.5044997472609936 1107 46-47 0.6652742388085855 1107 48-49 0.41733095210208404 1107 50-51 0.8400506094747442 1107 52-53 0.4859969089769294 1107 54-55 0.4388751742667978 1107 56-57 0.7657200115574199 1107 58-59 0.6300314434271925 1107 60-61 0.9369613127231631 1107 62-63 0.6971934211521571 1107 64-65 0.5618628787956155 1107 66-67 -0.1924961748659726 1107 68-69 0.8089536482319204 1107 70-71 -0.1267382757307871 1107 72-73 0.7497441501520825 1107 74-75 -0.41621662676615045 1107 76 0.9886706389623896 1108 1 -0.609251372262996 1108 2 0.29840220003087126 1108 3 -0.165996840961391 1108 4 0.34906587348170603 1108 5 0.13204836233765604 1108 6 -0.20377626408836846 1108 7 -0.2145347143020686 1108 8 -0.7925797040564078 1108 9 -0.6356506057303406 1108 10-11 0.27577133675855947 1108 12-13 0.8779939549385531 1108 14-15 0.2278791661608679 1108 16-17 -0.4316769144634236 1108 18-19 0.06630076967291387 1108 20-21 0.5986906071921894 1108 22-23 -0.24074202174386983 1108 24-25 0.6040294506505646 1108 26-27 -0.7397458930662069 1108 28-29 -1.2120067693036418 1108 30-31 -0.10159590808127916 1108 32-33 0.3567797310937664 1108 34-35 0.4456061503158324 1108 36-37 1.0054271844496583 1108 38-39 0.8022428712698897 1108 40-41 0.39748884429591413 1108 42-43 -0.5094144811886494 1108 44-45 0.6456951598522593 1108 46-47 0.5987533681652408 1108 48-49 0.901712409603121 1108 50-51 0.5588221344781914 1108 52-53 -0.2846326543238007 1108 54-55 -0.5736093277307219 1108 56-57 0.18600861880359787 1108 58-59 0.2524570530152914 1108 60-61 0.8204679940842254 1108 62-63 0.643863072619304 1108 64-65 0.3665363875433698 1108 66-67 0.8021570142785279 1108 68-69 1.257147269942898 1108 70-71 -0.8255776475450887 1108 72-73 -0.5440377068530182 1108 74-75 -1.1812582991728178 1108 76 0.725738416893229 1109 1 -1.1204817903561626 1109 2 0.3026730441709944 1109 3 0.2840498443794104 1109 4 0.26421958928902 1109 5 0.09408626391803665 1109 6 -0.3938979917917962 1109 7 -0.03571256815966706 1109 8 0.21760461658543306 1109 9 0.5980095249886119 1109 10-11 0.5031030101686653 1109 12-13 0.48458782705530723 1109 14-15 0.6214279436965668 1109 16-17 0.6020921487174036 1109 18-19 0.6931508924380765 1109 20-21 0.8415581245695094 1109 22-23 0.30723581136467715 1109 24-25 -0.24773594686647016 1109 26-27 0.28094262925630886 1109 28-29 -0.826506890339715 1109 30-31 0.3683568878519097 1109 32-33 0.44134071598155344 1109 34-35 -0.011802121634922003 1109 36-37 0.7632426563174235 1109 38-39 0.6429161776294663 1109 40-41 0.2673102296401737 1109 42-43 0.8588420806953607 1109 44-45 0.7921228667374791 1109 46-47 0.7005403792659806 1109 48-49 0.5908788169067805 1109 50-51 -0.07554086998284504 1109 52-53 -0.09796045457970592 1109 54-55 -0.7639515139879336 1109 56-57 0.138510607253302 1109 58-59 -0.10221321015170304 1109 60-61 0.730188054948087 1109 62-63 0.07497625863445023 1109 64-65 0.4965420935294702 1109 66-67 0.15539645820425108 1109 68-69 -0.00128635041882319 1109 70-71 0.0172440490347725 1109 72-73 0.004428593247276069 1109 74-75 0.19946930050902978 1109 76 0.42448828721173015 1110 1 -0.8679529173971794 1110 2 -0.7112390758343139 1110 3 0.07831507735431842 1110 4 0.19220312838366738 1110 5 0.26988607806910636 1110 6 0.0616841136111006 1110 7 0.5396624484339938 1110 8 0.7582922576314957 1110 9 0.9438876611893292 1110 10-11 -0.39257600217701594 1110 12-13 0.5778109785221872 1110 14-15 0.8108013672993835 1110 16-17 0.2842307389205061 1110 18-19 -0.09136390910182257 1110 20-21 0.928933672160106 1110 22-23 -0.5041806229908232 1110 24-25 -0.7670376858814691 1110 26-27 1.0307700552486097 1110 28-29 0.3533948752251348 1110 30-31 0.009615350615746365 1110 32-33 0.7105296181453582 1110 34-35 0.7209404775951356 1110 36-37 1.1788075043195434 1110 38-39 0.9525139474422915 1110 40-41 -0.7049982795859009 1110 42-43 0.31957485648189987 1110 44-45 0.8814365531837822 1110 46-47 0.8128064087363143 1110 48-49 1.2430492669266968 1110 50-51 0.0013607628327605426 1110 52-53 0.6784964682929839 1110 54-55 -0.29090624649822416 1110 56-57 0.5996482682117517 1110 58-59 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-3.733975616125072 2116 40-41 -0.5980153573847602 2116 42-43 -0.7644985148020922 2116 44-45 -0.11909475611413001 2116 46-47 -0.6279273041036575 2116 48-49 0.021670392796401927 2116 50-51 -0.5094551764461741 2116 52-53 -0.048246099701955814 2116 54-55 -0.22188663865508573 2116 56-57 -0.18533591901152136 2116 58-59 -2.1445177369006743 2116 60-61 -1.1159605773443388 2116 62-63 0.45831685413190826 2116 64-65 -1.3522451250616712 2116 66-67 -0.41217071681390394 2116 68-69 -0.7056678561075245 2116 70-71 1.0126156297658326 2116 72-73 0.2643656544915274 2116 74-75 0.06269128065911644 2116 76 -0.8597237679807179 2117 1 0.7746672895413482 2117 2 0.5243614516560484 2117 3 0.24212112320540768 2117 4 -0.3305956137546957 2117 5 0.12388283197456218 2117 6 0.35300588273262434 2117 7 0.6266932863321415 2117 8 0.2745480904576283 2117 9 -1.2057116490155835 2117 10-11 -0.8209565053216679 2117 12-13 -1.7755061243384347 2117 14-15 -0.05555590598120119 2117 16-17 -0.6697457268940639 2117 18-19 -1.094254962053732 2117 20-21 -0.28332540676056084 2117 22-23 -0.10972687983804974 2117 24-25 -1.48846698188472 2117 26-27 -2.028859179890368 2117 28-29 -1.6441654818452633 2117 30-31 -0.1163711578038118 2117 32-33 -2.148650742982653 2117 34-35 0.40306413350911185 2117 36-37 -0.9277125808904358 2117 38-39 0.08184291883608097 2117 40-41 -0.13909748835035174 2117 42-43 0.13337329429889877 2117 44-45 -0.4551352666579689 2117 46-47 -0.8147752508295198 2117 48-49 -0.14159264826908924 2117 50-51 -0.3563613917624906 2117 52-53 0.423013611729786 2117 54-55 -0.4157490137938211 2117 56-57 0.08680337066661536 2117 58-59 -1.0052724538818083 2117 60-61 -0.6402502554797422 2117 62-63 -0.5710172191200371 2117 64-65 -1.3859410185133854 2117 66-67 -0.40375506753532875 2117 68-69 0.23618841470268848 2117 70-71 -0.5083111182918785 2117 72-73 1.0607169308511288 2117 74-75 0.7386124793272586 2117 76 0.49300098229674916 2118 1 0.0056809020165786706 2118 2 0.484504038026337 2118 3 -0.5599379790604715 2118 4 0.1625227630868693 2118 5 0.24964745676994937 2118 6 0.21064566906845528 2118 7 0.7332992137897136 2118 8 0.43342850744737405 2118 9 -1.7847910838393943 2118 10-11 -0.4133522578847746 2118 12-13 -0.6817265085893212 2118 14-15 0.49110774526374 2118 16-17 -2.4893588913637785 2118 18-19 -0.35325814978527603 2118 20-21 -0.19201197521064017 2118 22-23 0.7902957358249125 2118 24-25 0.8399662911401791 2118 26-27 -2.6208133885575506 2118 28-29 -0.39908708088032796 2118 30-31 -0.5121183362306212 2118 32-33 0.44224268339797845 2118 34-35 -0.05612536420778014 2118 36-37 -2.4409965905397897 2118 38-39 0.09755003523135741 2118 40-41 -0.6038493622195702 2118 42-43 0.43853935788121845 2118 44-45 -1.7269593814163002 2118 46-47 0.102451422729537 2118 48-49 0.2588984346980894 2118 50-51 -0.7602609782189376 2118 52-53 -0.9936422855735643 2118 54-55 -0.5482121825712838 2118 56-57 0.37052763188828663 2118 58-59 -0.4379423944349199 2118 60-61 -0.33311878309238807 2118 62-63 -0.8517529820217291 2118 64-65 0.40969954321659685 2118 66-67 0.4522423908949307 2118 68-69 -0.9625440312351046 2118 70-71 -0.12243406134348334 2118 72-73 1.189050048797192 2118 74-75 0.4047514471914795 2118 76 0.32014193172382477 2119 1 0.6621916877196128 2119 2 -0.9368919176161938 2119 3 -0.08239788413728633 2119 4 -0.20352757769186525 2119 5 -0.20502784745660563 2119 6 -0.6057611959921587 2119 7 -0.513433584194857 2119 8 0.1738068505654411 2119 9 -0.16991603604039796 2119 10-11 -0.2990620448699488 2119 12-13 -0.1260266188081829 2119 14-15 0.005334976071033282 2119 16-17 -0.24050044387519165 2119 18-19 -0.1739556777336304 2119 20-21 -1.247948048269997 2119 22-23 -0.16985677109188657 2119 24-25 0.774487288958305 2119 26-27 0.38739116865503576 2119 28-29 0.11811493455031297 2119 30-31 1.0023956885573782 2119 32-33 0.5164435966399878 2119 34-35 0.35965280345985207 2119 36-37 -0.12533201578215625 2119 38-39 -1.0930048830042338 2119 40-41 -0.8688470003867863 2119 42-43 0.0021401406600887185 2119 44-45 0.2881900888583431 2119 46-47 -0.05341267066408051 2119 48-49 -0.25603172253249085 2119 50-51 0.5738540092970865 2119 52-53 0.10315349461853884 2119 54-55 0.2811791393802707 2119 56-57 -0.9861878459892139 2119 58-59 -0.36678374675288694 2119 60-61 -0.0659055208389816 2119 62-63 -0.4032971707884059 2119 64-65 -0.16124541483274868 2119 66-67 0.2942245309403688 2119 68-69 0.3462880986881913 2119 70-71 -0.46050230706407547 2119 72-73 0.7382717686613347 2119 74-75 -0.06730582163007881 2119 76 0.04557324737714907 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 4.0 21 8.0 22 14.0 23 24.0 24 48.0 25 90.0 26 192.0 27 370.0 28 709.0 29 1188.0 30 2106.0 31 3266.0 32 5105.0 33 8264.0 34 13277.0 35 21647.0 36 39522.0 37 71692.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.66504303809558 21.737521339971106 11.853085491207334 27.744350130725977 2 19.203586308990843 18.73858386160954 36.67430727170708 25.38352255769254 3 18.1142031684634 24.14311808316321 28.777025655719115 28.965653092654275 4 13.698769146281773 17.620548452180557 38.44656948772131 30.234112913816364 5 13.07916383128589 36.877857765361796 36.88382698804962 13.1591514153027 6 29.118465193462505 39.257189928727485 17.164499838830988 14.459845038979024 7 29.144729773288923 28.735838019173144 22.91286128720318 19.206570920334755 8 24.82062485823096 35.405250528276206 19.444145983310055 20.329978630182776 9 23.817795446676936 15.556391246731852 22.065231665532515 38.5605816410587 10-11 25.05521530986235 25.846734238267494 27.496328928046992 21.601721523823166 12-13 24.702434249012093 23.416962143189714 26.68123157002495 25.199372037773244 14-15 21.759607463916048 25.35875028353808 23.819287752348888 29.062354500196985 16-17 22.495314160190063 27.893879159055906 26.720926900898966 22.889879779855068 18-19 22.102240846197006 27.56885498370402 27.35038143332975 22.978522736769218 20-21 21.60261690722634 27.6933132767451 26.72122536203336 23.9828444539952 22-23 22.32160977997445 27.39335983668207 27.84761768322529 22.43741270011819 24-25 22.48456955935198 28.100115802920143 26.95283120232083 22.46248343540704 26-27 21.558444659336462 28.277700177882835 27.57213805618232 22.591717106598377 28-29 22.560378687487315 27.00774805104879 28.414395377433948 22.017477884029944 30-31 22.100748540525053 27.18652627054905 28.278595561286007 22.43412962763989 32-33 21.39817103016845 28.2421833028903 27.562885761016197 22.79675990592505 34-35 22.783329154877453 27.451559757888326 27.951780619127774 21.813330468106443 36-37 22.574406360803696 27.319043014218686 27.000883444957797 23.105667180019818 38-39 21.627090720246407 27.732710146484724 27.923725272495016 22.71647386077385 40-41 21.871530389312703 26.27920442200017 28.82657020402803 23.022694984659097 42-43 22.023148645583372 26.011484784451365 28.874622446664993 23.090744123300265 44-45 20.732304239341953 26.386650430380953 29.13786516719793 23.743180163079163 46-47 21.462041712928144 25.36740565643542 29.54765230471688 23.622900325919556 48-49 20.6236643864236 25.700189821281473 30.15651301887468 23.519632773420245 50-51 19.85005312608192 26.04222628129365 29.963110203789263 24.144610388835165 52-53 19.383558373028663 26.166983035469123 30.691355371703498 23.758103219798716 54-55 18.71858696560534 27.085049484856082 29.978331721643208 24.218031827895373 56-57 19.091364922459796 26.563040960806084 29.39036328689278 24.955230829841337 58-59 18.67680240679059 26.701228466029153 29.520790802621683 25.101178324558575 60-61 19.391915284791615 26.808972935544332 28.670176569607104 25.128935210056945 62-63 18.422811981423777 27.108926375607368 28.332915487745186 26.135346155223665 64-65 19.265367763809795 27.84433461074699 26.649594689779498 26.24070293566372 66-67 19.029285006506452 27.652424101333523 26.232346023900767 27.085944868259254 68-69 18.825436051717347 27.51334121270728 26.297709012332415 27.36351372324296 70-71 19.646204171292815 27.413953654955055 25.96462638635197 26.975215787400163 72-73 20.069422059859367 27.081766412377778 25.465897830784474 27.382913696978378 74-75 20.757971896899583 27.12922173274596 25.35367644425343 26.75912992610102 76 21.290426560653273 27.25368002578704 25.390685624917925 26.065207788641764 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 5.0 17 8.0 18 7.5 19 9.0 20 29.0 21 47.0 22 54.5 23 90.5 24 133.0 25 147.0 26 238.5 27 434.0 28 626.0 29 714.0 30 936.5 31 1378.0 32 2074.0 33 2551.0 34 2878.5 35 3610.0 36 4371.5 37 4729.0 38 5571.0 39 7262.0 40 9394.0 41 11124.5 42 11572.0 43 11715.5 44 12395.5 45 13048.5 46 13165.0 47 13059.0 48 12428.0 49 11344.0 50 10785.0 51 10195.5 52 8445.0 53 6453.0 54 5622.0 55 5276.5 56 4226.0 57 3047.5 58 2574.0 59 2022.0 60 1232.0 61 828.0 62 662.0 63 536.5 64 352.0 65 256.5 66 186.0 67 152.0 68 122.5 69 71.0 70 30.0 71 11.0 72 11.5 73 9.0 74 3.0 75 0.0 76 0.0 77 0.5 78 1.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 167526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.06562563422991 #Duplication Level Percentage of deduplicated Percentage of total 1 59.216417193474626 18.395950479328583 2 13.254424226120708 8.235139620118668 3 7.668658609226986 7.146950324128793 4 4.490517456718482 5.580029368575625 5 2.903368368464539 4.509747740649213 6 1.9676037123148165 3.667490419397586 7 1.4660953442345752 3.1881618375655125 8 1.2701035682032167 3.156524957320058 9 0.947293584151567 2.6485441065864403 >10 6.577253425052361 37.48254002363812 >50 0.21520665603443306 4.349175650346812 >100 0.021136368003381818 1.0338693695306997 >500 0.0 0.0 >1k 0.001921488000307438 0.6058761028138916 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT 1015 0.6058761028138916 No Hit ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT 392 0.2339935293626064 RNA PCR Primer, Index 24 (95% over 23bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 264 0.15758747895849 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.002984611343910796 0.0 2 0.0 0.0 0.0 0.002984611343910796 0.0 3 0.0 0.0 0.0 0.002984611343910796 0.0 4 0.0 0.0 0.0 0.002984611343910796 0.0 5 0.0 0.0 0.0 0.002984611343910796 0.0 6 0.0 0.0 0.0 0.002984611343910796 0.0 7 0.0 0.0 0.0 0.0053723004190394325 0.0 8 0.0 0.0 0.0 0.006566144956603751 0.0 9 0.0 0.0 0.0 0.01432613445077182 0.0 10 0.0 0.0 0.0 0.02148920167615773 0.0 11 0.0 0.0 0.0 0.024473813020068527 0.0 12 0.0 0.0 0.0 0.025070735288850687 0.0 13 0.0 0.0 0.0 0.026264579826415006 0.0 14 0.0 0.0 0.0 0.02805534663276148 0.0 15 0.0 0.0 0.0 0.02805534663276148 0.0 16 0.0 0.0 0.0 0.02984611343910796 0.0 17 0.0 0.0 0.0 0.031636880245454434 0.0 18 0.0 0.0 0.0 0.03581533612692955 0.0 19 0.0 0.0 0.0 0.04357532562109762 0.0 20 0.0 0.0 0.0 0.04715685923379057 0.0 21 0.0 0.0 0.0 0.048947626040137054 0.0 22 0.0 0.0 0.0 0.05193223738404785 0.0 23 0.0 0.0 0.0 0.054319926459176486 0.0 24 0.0 0.0 0.0 0.0584983823406516 0.0 25 0.0 0.0 0.0 0.062079915953344555 0.0 26 0.0 0.0 0.0 0.07103374998507694 0.0 27 0.0 0.0 0.0 0.10028294115540275 0.0 28 0.0 0.0 0.0 0.1271444432505999 0.0 29 0.0 0.0 0.0 0.16713823525900456 0.0 30 0.0 0.0 0.0 0.20534126046106277 0.0 31 0.0 0.0 0.0 0.23936582978164583 0.0 32 0.0 0.0 0.0 0.2936857562408223 0.0 33 0.0 0.0 0.0 0.33547031505557345 0.0 34 0.0 0.0 0.0 0.3939686973962251 0.0 35 0.0 0.0 0.0 0.47753781502572734 0.0 36 0.0 0.0 0.0 0.6363191385217817 0.0 37 0.0 0.0 0.0 0.9335864283752969 0.0 38 0.0 0.0 0.0 1.3269582035027399 0.0 39 0.0 0.0 0.0 1.9250743168224633 0.0 40 0.0 0.0 0.0 2.643768728436183 0.0 41 0.0 0.0 0.0 3.5540751883289756 0.0 42 0.0 0.0 0.0 4.607642992729486 0.0 43 0.0 0.0 0.0 5.740601458878025 0.0 44 0.0 0.0 0.0 6.736864725475448 0.0 45 0.0 0.0 0.0 7.733724914341654 0.0 46 0.0 0.0 0.0 8.71387127968196 0.0 47 0.0 0.0 0.0 9.705956090397908 0.0 48 0.0 0.0 0.0 10.57746260281986 0.0 49 0.0 0.0 0.0 11.405990711889498 0.0 50 0.0 0.0 0.0 12.21959576423958 0.0 51 0.0 0.0 0.0 13.12035146783186 0.0 52 0.0 0.0 0.0 14.206153074746606 0.0 53 0.0 0.0 0.0 15.239425522008524 0.0 54 0.0 0.0 0.0 16.24762723398159 0.0 55 0.0 0.0 0.0 17.1573367716056 0.0 56 0.0 0.0 0.0 17.894535773551567 0.0 57 0.0 0.0 0.0 18.68008547926889 0.0 58 0.0 0.0 0.0 19.39639220180748 0.0 59 0.0 0.0 0.0 20.13120351467832 0.0 60 0.0 0.0 0.0 20.82064873512171 0.0 61 0.0 0.0 0.0 21.565607726561847 0.0 62 0.0 0.0 0.0 22.31355132934589 0.0 63 0.0 0.0 0.0 23.10924871363251 0.0 64 0.0 0.0 0.0 24.01358595083748 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTCGA 25 2.830404E-6 70.00001 60 ACGTCTC 25 2.830404E-6 70.00001 58 CTCGAGT 20 7.908891E-5 70.0 62 CGTCTCG 30 8.349009E-6 58.333332 59 TCTCGAG 30 8.349009E-6 58.333332 61 CGAGTGA 25 2.385124E-4 56.000004 64 CTCGAAC 25 2.385124E-4 56.000004 33 GAGGCGC 45 2.6850103E-8 54.444447 13 CGAACTG 20 0.0069417255 52.5 35 TGAAGGT 40 7.4456693E-7 52.5 28 TCTGTCA 20 0.0069417255 52.5 16 GTACTCG 20 0.0069417255 52.5 3 TACTCGT 20 0.0069417255 52.5 4 CGTCCAA 20 0.0069417255 52.5 17 CGTGCGA 35 2.0797732E-5 50.0 10 CGAGAAT 35 2.0797732E-5 50.0 14 TTCTGTT 35 2.0797732E-5 50.0 38 GCGTGAA 50 6.158916E-8 49.000004 25 CGCCATT 50 6.158916E-8 49.000004 17 TGGCGTG 45 1.6771173E-6 46.666668 23 >>END_MODULE