FastQCFastQC Report
Wed 25 May 2016
SRR1780623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTTGGCTCGCGAGAAGGCCTAGATAGGGGATTGTGCGGTGTGCTGTC11.694915254237288No Hit
TCGTGGGACTGCCCGTGTTCGTCCTGCCCGCCCTGCGCCTGGGCGTGTGG11.694915254237288No Hit
GCCCTCGGGGCTGTCCGTGGGCGCGGACGGCGCGCTGTGGGTCGCGGACT11.694915254237288No Hit
GGTTAGAAGTAGGGTCTTGGTGACAAAATATGTTGTGTAGAGTTCAGGGG11.694915254237288No Hit
GTTTGGGCGCGTCGGTGGCTGGCAAAGGGCGGCGCGGTGGGGCTGTCTCT11.694915254237288No Hit
TCGATAATCTGTTTTGTTGCTATCGTCGGGGACTGTGTTATCTGCGGACC11.694915254237288No Hit
ACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCAT11.694915254237288No Hit
CTCTTATACACATCTGACGCTGCCGACGAGAATGATCGTTTAGTTCTCGG11.694915254237288No Hit
TTGATCTTGGTCGTCTGATGAGGGAGTAGTTGGGAGAGTTGCTGTTGGGA11.694915254237288No Hit
CATATGACGCTGCCGACGAACCTCTAGGTGTAGATCTCGGGGGGCGCCGG11.694915254237288No Hit
CTTATACCCATCTGCCGCGGTGGACCGCCTCCTGGGGGGAAACCCCGGCG11.694915254237288No Hit
ATTGAAAGCAGGAAATCAAAGTGGCAAAGGTCATATGACTCTTGGGGTTC11.694915254237288No Hit
GGATTCAAGTGCAACCCAACACGTCCCTAGCTATGATCGCTAAGAGGGAA11.694915254237288No Hit
TCTTATACACATCTGACGCTGCCGACGAATGACCATGTGGGTTTCCTGGC11.694915254237288No Hit
GCTCGAGCACGAGGTCCGCCTCGCCGTCGAACCCGCCGAACGGGTCCGCG11.694915254237288No Hit
GGAAGAGCCAAGGAGGGGATGAGATGTGAGGGATCTGGGGGTCAGTTGAG11.694915254237288No Hit
AATTATAGAAGGAACAGGCACAGGGGGGGTCGGATTTTTGGGGTTATACG11.694915254237288No Hit
GCCTCATGAAGGGCTACTTCGACCGCTTCGGCGGCGTGCGCGACTACCTG11.694915254237288No Hit
GGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATC11.694915254237288No Hit
AGGTATTGGTCTCCTGGGCGTGGTGATTGGTCGGTCTGTCGGTGGCTGTC11.694915254237288No Hit
GTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGCGGATAGTTTGATT11.694915254237288No Hit
ATATTACTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTT11.694915254237288No Hit
GGCGAGCGCGCTCCGGTGACGCCGACGGCACGGGCCCTGTCTCTTATACA11.694915254237288No Hit
GCCGGGAGCGCATAGACATTCTCAACTTTTTGCTCATCCCTTGATGATAA11.694915254237288No Hit
CGCTCAGGCGGTGCGCAGCACCATGCCCGGGTGGAGCATGGTCGAGACCG11.694915254237288No Hit
GGGATGGAGGCGCCCGGCCGCGCGCCGGAGGCCCGCCCGCCCGCCGCCTG11.694915254237288No Hit
GACCCCCACCCACGTTTTCGTTCGGGAAACGGCGGAGCATCCTCCTCTCA11.694915254237288No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATATCGGGG11.694915254237288No Hit
CTACACGTCTGGCCCGGCCTACGTGGAGGAGCGCGGGGGCGGGCTCGTAC11.694915254237288No Hit
TTTTCGCTTCCCACAGGTCTCCTGTCTCTTATACACATCTGACGCTGCCG11.694915254237288No Hit
GAGCAAGAGCCGGTTAGTGTGATCGGTGTTTACCGATGACAGCACAGGCA11.694915254237288No Hit
GGACTGCCAGGGCGGGCGCCAGTCGGGGGCGGGGGCGGTCCTGTCTCTTA11.694915254237288No Hit
CTTATACACATCTGACGCTGCCGACGAATGACCATGGTTATGTCTCGGGG11.694915254237288No Hit
ATCTAGGAGAAGGTGAATGAAAAGTTTTATCCAGGCATGATCACCAGGGT11.694915254237288No Hit
GTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTA11.694915254237288No Hit
GGGGACCCGCCCCCGGGCCCGGGCGGGGGGGTAAAGGGGGGGGGGGGCTT11.694915254237288No Hit
ATTTTGTGTCAACTGGAGTTTTTTACAACTCAGGTGAGTTTTAGCTCTGT11.694915254237288No Hit
GTCTTGCAAGTACAGCAGCCACACCCAGGCAGGTCCCGTGCTCTCTCTTA11.694915254237288No Hit
CTTATACACATCGGACGCTGCCGACGAATCTGGGGGTGGAGGTCTCCGGG11.694915254237288No Hit
GAAAAAAGGGAAACAATCACTAAACCAGTTAGGTTCGGGTACTGCACCAA11.694915254237288No Hit
AGGCCAGCCAGGCCACGAACAGCACCGTCTTGGCCCCTGTCTCTTATACA11.694915254237288No Hit
CTACTCACCAGACGCCTCAACCGCCTTTTCATCAATCGCCCACATCACTC11.694915254237288No Hit
CCCATATAACCTCCCCCAAAATTCAGAATAATAACACACCCGACCACACC11.694915254237288No Hit
CGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGCT11.694915254237288No Hit
GGGATGGAGGCGCCCGGCCGCGCGCCGGAGGCCCGGCCGCCCGCCGCCTG11.694915254237288No Hit
CACTGGAATGGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAA11.694915254237288No Hit
AGCGTGAAAGATGGGAGGGAACGGAAAGAGGAGGGCGGGATAAGGTGAGC11.694915254237288No Hit
TCTTCTTACTCATCCGCTTCCACCCCCTAGCAGAAAATAGCCCACTAATC11.694915254237288No Hit
GTTCCGGCTGGCCTTCGGGATCGGCGACTTCCGCCGTGACACCGGCCTGG11.694915254237288No Hit
GCTCGTGCGGGGAGACCAGCACGTAGCCGCGCTCGGAGACCCTGTCTCTT11.694915254237288No Hit
CTTATACACATCTGCCGCTGCCGGCGCAACTGCCGGTGTAGATCGCGGCG11.694915254237288No Hit
CGCCGGTCCCGTGAGCGGTTCTTCGACGAGGACACCGTCGCCTTCGACGT11.694915254237288No Hit
ATTTTGCAATAGAATTCTAATTTGTACTGGGAGGGTTCTATTGAGGAACC11.694915254237288No Hit
GTGCTTGATGCTTGTCCCTTTTGATCGTGGTGATTTAGAGGGTGAACTCA11.694915254237288No Hit
CACGTGGTGCCCGGCGCTTTAGTGATCCAGGCACCTTCTCCGGGGCGAAT11.694915254237288No Hit
ACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATG11.694915254237288No Hit
TATTTGTTTGACCGGCGCTTGGGTATGGTGTGTGCTCCTATTAATAGCAA11.694915254237288No Hit
GCATTAGGAATGCCATTGCGATTAGAATGGGTACAATGAGGAGTAGGAGG11.694915254237288No Hit
TGGTGGACCTGTGCACCGGCTCCGGCGCGATCGCTGCGGCCGTCGCGACG11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACG50.044.01
ACACGTA50.044.03
CCTATCA50.044.037
AGCTCAC50.044.019
TCCTATC50.044.036
TGTCCTA50.044.034
TTGTAGC50.044.015
ACGTTGT50.044.012
GTACTAC50.044.07
CACGTAC50.044.04
TATGTCC50.044.032
ATAGGCT50.044.044
TAGCTCA50.044.018
ACGTACT50.044.05
TCAATAG50.044.041
GACACGT50.044.02
ACTTCCA50.044.024
CAATAGG50.044.042
TACGTTG50.044.011
CCACTAT50.044.028
Produced by FastQC (version 0.11.5)