FastQCFastQC Report
Wed 25 May 2016
SRR1780623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT35.084745762711865No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT23.389830508474576No Hit
CTTATACACATCTCCGAGCCCACGAGAACGGTTCCCATCTCGTATGCCGT11.694915254237288No Hit
CATATGACGCACTCTCCCCTGAACTCTACACAACATATTTTGTCACCAAG11.694915254237288No Hit
CCGCTACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGA11.694915254237288No Hit
CTTATACACATCTCCGAGCCACGAGACTTCGCGGCATCTCGTATGCCCTC11.694915254237288No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC11.694915254237288No Hit
TCGTGCCCCCGGCGCCGCGGCGCGAGAGCTCACGCAGCGCCAGACCGCCG11.694915254237288No Hit
CATACCCATGGCCAAACTCCTACTCCTCATTGTACCCATTCTAATCGCAA11.694915254237288No Hit
CGCCTCAACGGCATGACGGTCTCGACCATGCTCCACCCGGGCATGGTGCT11.694915254237288No Hit
TTATGGGGGTTTAGTATTGATTGTTAGCGGTGTGGTCGGGTGTGTTATTA11.694915254237288No Hit
CTACTCAACTGACCCCCAGATCCCTCACATCTCATCACCTCCTTGGCTCT11.694915254237288No Hit
GAGACCTGTGGGAAGCGAAAACTGTCTCTTATACACATCTCCGAGCCCAC11.694915254237288No Hit
CACACCGCACAATCCCCTATCTAGGCCTTCTCGCGAGCCAAAACCCTGTC11.694915254237288No Hit
ACCACGTCCGCGCCGTCGGCGGTGCCCGCCAGGACGCGGTCCACCGCGGC11.694915254237288No Hit
GTGTAGAGGACGTCGAAGGCGACGGTGTCCTCGTCGAAGAACCGCTCACG11.694915254237288No Hit
TACTTTTCCCTTTCCTTTTCTCAAGTAGAAATGTTCTTCCCTCTTAGCGA11.694915254237288No Hit
GCCCAGGCCGGTGTCACGGCGGAAGTCGCCGATCCCGAAGGCCAGCCCGA11.694915254237288No Hit
GGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACAC11.694915254237288No Hit
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG11.694915254237288Illumina PCR Primer Index 3 (96% over 28bp)
AGCCCACGAGACCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT11.694915254237288No Hit
CTTCTGATCAAATCCTAAGTCATACACCTGACCCTGGTGATCATGCCTGG11.694915254237288No Hit
GTGTATTAGTTTATACTACAAGGACAGGCCCATTTGAGTATTTTGTTTTC11.694915254237288No Hit
CTGATAAACTAAGAGAGAGCACGGGACCTGCCTGGGTGTGGCTGCTGTAC11.694915254237288No Hit
GGGCCAAGACGGTGCTGTTCGTGGCCTGGCTGGCCTCTGTCTCTTATACA11.694915254237288No Hit
ATCTCCGAGCCACGAGACCCAACACGATCTCGTATGCCGTCTTCTGCTTG11.694915254237288TruSeq Adapter, Index 1 (96% over 31bp)
AGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGACACAAAATCTGT11.694915254237288No Hit
GGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCG11.694915254237288No Hit
ATTACCTTCTTATTATTTGATCTAGAAATTGCCCTCCTTTTACCCCTACC11.694915254237288No Hit
ATACACATCTCAGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT11.694915254237288RNA PCR Primer, Index 27 (95% over 22bp)
CCTCTCCGCCGCCCTGCTGCTCGAGCACGTGGGCGAGCTGTCTCTTATAC11.694915254237288No Hit
TTGGTGAAGAGTTTTATGGCGTCAGCGAAGGGTTGTAGTAGCCCGTAGGG11.694915254237288No Hit
ATTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCAT11.694915254237288No Hit
GGTCTACGAGCGCGGCTACGTGCTGGTCTCCCCGCACGAGCCTGTCTCTT11.694915254237288No Hit
CTTATACACATTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC11.694915254237288No Hit
GCCCGCCGTTTCCCGAACGAAAACGTGGGTTTGGGCCCTGTGTCTTATAC11.694915254237288No Hit
CTTATACACATCTCCAAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11.694915254237288No Hit
ACGCCCAGCGGGTGCTGCAGGCGCGCGCGGTCCGCCTCCCCGTCCACGTG11.694915254237288No Hit
CTTATACACATCTACGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT11.694915254237288No Hit
CTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTAT11.694915254237288No Hit
GGCCCGTGCCGTCGGCGTCACCGGAGCGCTCTCGCCCTGTCTCTTATACA11.694915254237288No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGCT11.694915254237288No Hit
ATTCCAATCCATTCAATTCCATTCCATTCGTGTACATTCCTTTCGAGTCC11.694915254237288No Hit
GCCACGATCAGCACCCACCACGGCACGGTGGGCAGCCCGGGCGCGAGCCC11.694915254237288No Hit
CCCTACAGCGCCGCCTTTTTCTCTGCAGCCCCTCCCCCACCCCTGTTTCT11.694915254237288No Hit
ATCTCCGAGCCCACGAGCGATCCAAAATCTCGTATGCCGTCTTCTGCTTG11.694915254237288TruSeq Adapter, Index 6 (96% over 29bp)
TCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTTCTTCTTG11.694915254237288Illumina PCR Primer Index 3 (96% over 27bp)
GCCCTGGTGTCCGTGGCGGAGGAGCTCGCGGACCCGTTCGGCGGGTTCGG11.694915254237288No Hit
ATCTCCGAGCCCACCGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCT11.694915254237288RNA PCR Primer, Index 36 (96% over 26bp)
AACCCCAAGAGTCATATGACCTTTGCCACTTTGATTTCCTGCTTTCAATC11.694915254237288No Hit
GGACCTCGAGGATGAGCTCGTCGTGGACCTGCAGCAGCAGGCGCGAGGCC11.694915254237288No Hit
TCACTACCTCGTCTCCGAGGGCCACCGGGTGTGTCTTCGTACCGTGGGAC11.694915254237288No Hit
GTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGC11.694915254237288No Hit
ACCCCGCCTATTGGAGACGGACTGCTGAAGCCCACCCCCGAGCCCACGAG11.694915254237288No Hit
CGCCTAAGCATAGTGTTAGAGTTTGGATTAGTGGGCTATTTTCTGCTAGG11.694915254237288No Hit
GACCACCCCCGCCCCCGACTGGCGCCCGCCCTGGCAGTCCCTGTCTCTTA11.694915254237288No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAAC50.044.029
AACGTAG50.044.033
CCTATTG50.044.01
GTGTCGC50.044.043
TAGGACA50.044.09
TAGTGGA50.044.016
AGGACAT50.044.010
AGCTACA50.044.027
GAAGTGA50.044.021
ATAGGAC50.044.08
AGTGGAA50.044.017
GGACATA50.044.011
ACGTAGT50.044.034
CGTAGTA50.044.035
GAGCTAC50.044.026
TATTGAT50.044.03
TGATAGG50.044.06
GTGAGCT50.044.024
GCTACAA50.044.028
AGTACGT50.044.038
Produced by FastQC (version 0.11.5)