FastQCFastQC Report
Wed 25 May 2016
SRR1780622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26708
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT10493.9276621237082523No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT4541.6998652089261643TruSeq Adapter, Index 12 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC1290.48300134791073834No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG850.31825670211172685No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC740.2770705406619739No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC480.17972143178073985RNA PCR Primer, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT390.14602366332185113No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT350.1310468773401228RNA PCR Primer, Index 12 (95% over 22bp)
GTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGT270.10109330537666616No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCACA150.002201114570.017
ACGTCTC150.002201114570.058
AATTTAC150.002201114570.053
TATCGTA150.002201114570.038
ATGGCAC150.002201114570.016
TCTCGAG150.002201114570.061
GTCTCGA150.002201114570.060
CGAGTGA150.002201114570.064
GTGATGT150.002201114570.067
AATATCG150.002201114570.036
ATCGTAT207.787866E-570.039
ATATCGT150.002201114570.037
TCGAGTG150.002201114570.063
ATTCAGC207.787866E-570.043
CGTCTCG150.002201114570.059
CTCGAGT150.002201114570.062
CAGCCAT252.3488431E-456.00000446
TGATTCA252.3488431E-456.00000441
AAGGTAG252.3488431E-456.00000430
TTCAGCC252.3488431E-456.00000444
Produced by FastQC (version 0.11.5)