##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780622_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26708 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.88527781937996 34.0 34.0 34.0 31.0 34.0 2 33.296390594578405 34.0 34.0 34.0 33.0 34.0 3 33.51726074584394 34.0 34.0 34.0 34.0 34.0 4 33.48157855324247 34.0 34.0 34.0 34.0 34.0 5 33.48498577205332 34.0 34.0 34.0 34.0 34.0 6 37.25685187958664 38.0 37.0 38.0 36.0 38.0 7 36.849033997304176 38.0 37.0 38.0 35.0 38.0 8 36.87962408267186 38.0 37.0 38.0 35.0 38.0 9 36.70686685637262 38.0 37.0 38.0 34.0 38.0 10-11 36.403081473715744 38.0 37.0 38.0 34.0 38.0 12-13 36.26892691328441 38.0 37.0 38.0 34.0 38.0 14-15 36.52727647146922 38.0 37.0 38.0 34.0 38.0 16-17 36.64124981279018 38.0 37.0 38.0 34.0 38.0 18-19 36.633162348360045 38.0 37.0 38.0 34.0 38.0 20-21 36.40141530627527 38.0 37.0 38.0 34.0 38.0 22-23 36.46077954171035 38.0 37.0 38.0 34.0 38.0 24-25 35.77914856971694 38.0 36.5 38.0 32.0 38.0 26-27 36.03100194698217 38.0 37.0 38.0 33.0 38.0 28-29 35.91560581099296 38.0 37.0 38.0 32.0 38.0 30-31 35.565354949827764 38.0 36.5 38.0 31.5 38.0 32-33 36.07932080275573 38.0 37.0 38.0 34.0 38.0 34-35 36.005822225550396 38.0 37.0 38.0 33.0 38.0 36-37 36.18054515500974 38.0 37.0 38.0 34.0 38.0 38-39 35.68331586041636 38.0 36.5 38.0 32.5 38.0 40-41 35.40094728171334 38.0 36.0 38.0 30.0 38.0 42-43 35.857495881383855 38.0 36.5 38.0 32.5 38.0 44-45 36.089355249363486 38.0 37.0 38.0 33.5 38.0 46-47 36.32943312865059 38.0 37.0 38.0 34.0 38.0 48-49 36.096413059757374 38.0 37.0 38.0 33.5 38.0 50-51 36.2083645349708 38.0 37.0 38.0 34.0 38.0 52-53 36.12707802905497 38.0 37.0 38.0 34.0 38.0 54-55 36.332671858619136 38.0 37.0 38.0 34.0 38.0 56-57 36.37249138834806 38.0 37.0 38.0 34.0 38.0 58-59 36.37438220757825 38.0 37.0 38.0 34.0 38.0 60-61 36.08435674704209 38.0 37.0 38.0 33.0 38.0 62-63 36.353545754081175 38.0 37.0 38.0 34.0 38.0 64-65 36.35154260895612 38.0 37.0 38.0 34.0 38.0 66-67 35.6126254305826 38.0 36.5 38.0 31.0 38.0 68-69 34.85451924516998 38.0 35.5 38.0 26.0 38.0 70-71 34.5363935899356 38.0 35.0 38.0 24.5 38.0 72-73 34.01675527931706 37.5 34.0 38.0 22.5 38.0 74-75 34.489628575707656 38.0 35.5 38.0 24.5 38.0 76 34.00883630372922 37.0 34.0 38.0 23.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.5412846817120425 1101 2 0.19233992523786014 1101 3 -0.4501124252189399 1101 4 0.005742013798744949 1101 5 -0.49027627436255017 1101 6 -1.8013915813471115 1101 7 0.020781697755431594 1101 8 0.20340095438400851 1101 9 0.0480594838164663 1101 10-11 -0.48447574341629007 1101 12-13 0.07419620475144484 1101 14-15 0.29295868254913415 1101 16-17 -0.6006345340671899 1101 18-19 -1.4389624710576996 1101 20-21 0.09199419841327483 1101 22-23 0.31362081790469176 1101 24-25 -2.613176660650545 1101 26-27 -0.7911337464353494 1101 28-29 -0.412753381686521 1101 30-31 0.8321598119289106 1101 32-33 -0.8581754312437582 1101 34-35 -0.8009505942008488 1101 36-37 -0.8127066449271254 1101 38-39 -1.172936687686125 1101 40-41 0.6658155906280854 1101 42-43 0.3007141409938683 1101 44-45 0.4844605182051751 1101 46-47 -0.3370577303734734 1101 48-49 -1.8945444584352558 1101 50-51 -1.923984124303864 1101 52-53 -2.292629699472535 1101 54-55 -2.1406766703747593 1101 56-57 -1.6946115089290927 1101 58-59 -1.8454077663761126 1101 60-61 -1.6403829819411158 1101 62-63 -2.2000165765704196 1101 64-65 -2.0528244312802357 1101 66-67 -2.1643636079968758 1101 68-69 -2.7495195514095343 1101 70-71 -2.767710327581728 1101 72-73 -1.8696352283785984 1101 74-75 -1.0806577468326424 1101 76 -2.1429483599126726 1102 1 -0.8042784447058082 1102 2 0.27362890652684513 1102 3 0.24323476812825362 1102 4 0.4845361925929197 1102 5 0.424900440814163 1102 6 0.5944504144948866 1102 7 0.4933388703126056 1102 8 0.4782450292280842 1102 9 0.6068952426522287 1102 10-11 0.28745772851718243 1102 12-13 0.2944664750217214 1102 14-15 -0.3728417332512848 1102 16-17 0.0750411416084873 1102 18-19 -0.15944064153587334 1102 20-21 0.6420981485172348 1102 22-23 0.6448058490897282 1102 24-25 -5.458291005764888 1102 26-27 -1.3758530811546805 1102 28-29 0.7526312336980965 1102 30-31 1.5628250925941956 1102 32-33 0.6156291425608202 1102 34-35 0.7578851646349136 1102 36-37 -2.4419166241371073 1102 38-39 0.06282214807271203 1102 40-41 0.7187261935386857 1102 42-43 1.1322110224907576 1102 44-45 1.345375279119935 1102 46-47 1.165541022225284 1102 48-49 -3.6688687827595814 1102 50-51 -0.9252315255512613 1102 52-53 -0.05843011527295516 1102 54-55 0.37013414043605053 1102 56-57 0.5657835014659227 1102 58-59 0.07486250389415972 1102 60-61 -6.225414166972293 1102 62-63 -2.287646514200354 1102 64-65 0.2117285832727731 1102 66-67 0.8506052069719416 1102 68-69 -0.1522222541122389 1102 70-71 -1.5959847558561577 1102 72-73 -5.716308825052195 1102 74-75 -2.2444831106580097 1102 76 -0.16768848465279973 1103 1 0.3140999336725727 1103 2 0.3984937713917063 1103 3 0.11296449785798046 1103 4 0.20471637277309895 1103 5 0.11895449486821974 1103 6 0.15553149557596413 1103 7 0.22180733878107617 1103 8 0.378785569768624 1103 9 -0.06373538797840439 1103 10-11 -0.015244974185520732 1103 12-13 -1.6991371285818815 1103 14-15 -1.2880669584765059 1103 16-17 -0.36063453406718793 1103 18-19 0.3615503494551149 1103 20-21 -0.28492887850980253 1103 22-23 0.39669774098161525 1103 24-25 -3.2218946093684906 1103 26-27 -0.3521593874609863 1103 28-29 -0.7840354329685653 1103 30-31 0.05318545295455124 1103 32-33 0.9464399533716232 1103 34-35 -0.3594121326623849 1103 36-37 -1.4439886962091748 1103 38-39 -0.8811418158912474 1103 40-41 -1.2546972298847408 1103 42-43 -0.44697816669843604 1103 44-45 0.6675374412820929 1103 46-47 0.36653201321627193 1103 48-49 -3.0278777917685957 1103 50-51 -1.7275738678936037 1103 52-53 -0.7669886738315057 1103 54-55 -1.7873433370414205 1103 56-57 -0.10025253457011729 1103 58-59 -1.0387410997094477 1103 60-61 -5.170639392197522 1103 62-63 -1.893862730416572 1103 64-65 -0.8420552005110054 1103 66-67 -1.4212866849199557 1103 68-69 -0.236186218076206 1103 70-71 -0.7246334045048073 1103 72-73 -6.017840356583726 1103 74-75 -3.849888516063409 1103 76 -5.155256052220366 1104 1 -0.0038221442495043334 1104 2 0.5216106545085921 1104 3 0.3820554069488864 1104 4 0.27727048532721454 1104 5 -0.30026628435255986 1104 6 -0.07771525767078913 1104 7 -0.1312662542925196 1104 8 0.49930505028810757 1104 9 0.8680395037964885 1104 10-11 -0.05810211704265811 1104 12-13 0.267702698257942 1104 14-15 0.37487676446721707 1104 16-17 0.4123524789198214 1104 18-19 0.6616369295416931 1104 20-21 -0.15765615123707022 1104 22-23 0.03020423448811016 1104 24-25 0.5571530096791264 1104 26-27 1.1998752445736471 1104 28-29 1.333800064866928 1104 30-31 0.46972224949134755 1104 32-33 0.5628035897352675 1104 34-35 -0.3446069378571863 1104 36-37 0.967613035392553 1104 38-39 1.1161742014247622 1104 40-41 1.6859954108079052 1104 42-43 0.5566581969379243 1104 44-45 0.6698751036197592 1104 46-47 1.174930281614543 1104 48-49 -0.5101288740196779 1104 50-51 0.6198720195522824 1104 52-53 0.0983792915364603 1104 54-55 -0.3627545924526743 1104 56-57 0.009184694867109044 1104 58-59 -0.5363168572852075 1104 60-61 0.415161473603348 1104 62-63 -0.48762896418280377 1104 64-65 0.18443830598249633 1104 66-67 -0.1711568147900806 1104 68-69 1.6154155135255266 1104 70-71 2.2553665954951967 1104 72-73 1.103891245147878 1104 74-75 0.6682933021183999 1104 76 -0.40408722105153316 1105 1 0.8240999336725707 1105 2 0.3384937713917111 1105 3 0.31046449785797847 1105 4 0.36054970610643267 1105 5 -0.446045505131778 1105 6 0.2255314955759644 1105 7 0.8159740054477425 1105 8 0.018785569768624555 1105 9 0.8720979453549305 1105 10-11 0.841005025814475 1105 12-13 0.499196204751442 1105 14-15 0.7040163748568276 1105 16-17 0.48061546593280724 1105 18-19 0.658633682788448 1105 20-21 0.39007112149020884 1105 22-23 0.38669774098161014 1105 24-25 1.3839387239648389 1105 26-27 0.7574239458723468 1105 28-29 0.8963812336980936 1105 30-31 1.133602119621223 1105 32-33 1.4014399533716215 1105 34-35 0.5355878673376182 1105 36-37 0.7714279704574949 1105 38-39 0.3030248507754152 1105 40-41 0.5869694367819278 1105 42-43 0.7805218333015631 1105 44-45 1.063787441282102 1105 46-47 1.2961153465496054 1105 48-49 0.3179555415647428 1105 50-51 0.9490927987730586 1105 52-53 0.5184279928351572 1105 54-55 0.9343233296252436 1105 56-57 0.7630807987632124 1105 58-59 0.8733422336238874 1105 60-61 0.8639439411358154 1105 62-63 -0.19136273041657148 1105 64-65 0.5529447994889907 1105 66-67 0.42746331508004687 1105 68-69 0.8129804485904657 1105 70-71 1.6803665954951938 1105 72-73 1.8813263100829474 1105 74-75 1.0501114839365897 1105 76 1.289743947779634 1106 1 -0.07658499783428141 1106 2 0.035754045364313924 1106 3 0.22638915539222637 1106 4 0.0459949115858862 1106 5 0.1912832619915079 1106 6 -0.09159179209526513 1106 7 -1.1044027068810252 1106 8 0.0735800903165682 1106 9 -0.9232787669738372 1106 10-11 0.543659135403523 1106 12-13 1.239949629408983 1106 14-15 -0.18596650185550345 1106 16-17 0.46785861661773964 1106 18-19 0.4559795731994072 1106 20-21 0.20411221738061158 1106 22-23 0.659985412214489 1106 24-25 0.08650721711553189 1106 26-27 1.1408999732696046 1106 28-29 0.22865863095836403 1106 30-31 0.8809480100321778 1106 32-33 -0.06671073155987983 1106 34-35 0.5359303330910379 1106 36-37 1.2177464636081723 1106 38-39 0.17648375488500534 1106 40-41 0.527037929932618 1106 42-43 1.0217889565892335 1106 44-45 1.4390442905971668 1106 46-47 1.219317401344128 1106 48-49 1.2890171854003611 1106 50-51 0.41964074397854034 1106 52-53 0.833924568177622 1106 54-55 0.6552137405841449 1106 56-57 0.4829438124618477 1106 58-59 0.8628114117060832 1106 60-61 1.0813240781221083 1106 62-63 1.3009317901313722 1106 64-65 -0.9384935566753896 1106 66-67 -0.11662915067337565 1106 68-69 1.016233873247998 1106 70-71 0.8786542667280699 1106 72-73 0.12773041967198395 1106 74-75 0.4049060044845305 1106 76 0.14830559161525514 1107 1 0.8372578284094132 1107 2 -0.05887464966092182 1107 3 0.26243818206850733 1107 4 0.1296286534748532 1107 5 0.2026387053945342 1107 6 0.29395254820754957 1107 7 0.6021582159740575 1107 8 -0.1391091670734781 1107 9 -1.150928370434542 1107 10-11 0.7821234468671108 1107 12-13 0.2807751521198725 1107 14-15 -0.26473362514317245 1107 16-17 0.5901549396170154 1107 18-19 0.9346205248937096 1107 20-21 0.845334279384943 1107 22-23 0.22090826729740343 1107 24-25 0.5477545134385267 1107 26-27 0.651173945872344 1107 28-29 0.8315786021191514 1107 30-31 -0.048305775115622396 1107 32-33 0.5382820586347847 1107 34-35 -0.2565173958202749 1107 36-37 0.7184674441417016 1107 38-39 0.22605116656488633 1107 40-41 0.8830220683608729 1107 42-43 0.8509165701436743 1107 44-45 0.19832691496630872 1107 46-47 0.5885495570759218 1107 48-49 0.6751923836700016 1107 50-51 0.9254085882467464 1107 52-53 0.5233622033614722 1107 54-55 0.8685338559410312 1107 56-57 0.6525544829737342 1107 58-59 0.7453817073080913 1107 60-61 1.2313781516621276 1107 62-63 0.7724530590571135 1107 64-65 1.2239974310679358 1107 66-67 0.11660805192214951 1107 68-69 1.8070593959588876 1107 70-71 0.22905080602151173 1107 72-73 1.0832999942934691 1107 74-75 -0.45252009501078305 1107 76 0.5147439477796354 1108 1 -0.4575456359476817 1108 2 0.10684820177145582 1108 3 0.27043285228835856 1108 4 0.4474167947140302 1108 5 0.3523722163872094 1108 6 -0.07130394746200608 1108 7 0.22973982823255312 1108 8 -0.7913410125098537 1108 9 -0.5764780040121593 1108 10-11 -0.27600446785640287 1108 12-13 0.6919177237387899 1108 14-15 -0.059037422611531554 1108 16-17 -0.6702547872317481 1108 18-19 0.016149505573260114 1108 20-21 0.8365901088319774 1108 22-23 -0.24178327167661706 1108 24-25 0.9170083442180044 1108 26-27 -0.7823703579251244 1108 28-29 -0.3454700321246875 1108 30-31 0.26390275253260853 1108 32-33 0.6542880546374477 1108 34-35 0.6251448293629309 1108 36-37 1.0798931603309043 1108 38-39 1.164259027990603 1108 40-41 0.7752605760224327 1108 42-43 -0.38925664771109325 1108 44-45 0.7923475678643754 1108 46-47 0.6955615490812477 1108 48-49 1.177607440298921 1108 50-51 3.586215578721408E-4 1108 52-53 0.1541083725819945 1108 54-55 -0.12422097417222489 1108 56-57 -0.19071666959121814 1108 58-59 0.1685162842567962 1108 60-61 0.9649724221484703 1108 62-63 0.8347448645201325 1108 64-65 -0.18914380810594622 1108 66-67 0.5093462264724522 1108 68-69 0.7530120941600842 1108 70-71 -0.5610891007073349 1108 72-73 -0.7742116646006068 1108 74-75 -1.213179655303918 1108 76 0.23309837815938295 1109 1 -0.45367784410520784 1109 2 0.47182710472504397 1109 3 0.37574227563575846 1109 4 0.30360526166199264 1109 5 -0.06271217179844513 1109 6 0.16164260668708152 1109 7 0.17014067211440675 1109 8 0.5743411253241817 1109 9 0.5595979453549305 1109 10-11 0.570866136925595 1109 12-13 0.7116962047514477 1109 14-15 1.0338774859679418 1109 16-17 1.1910321325994815 1109 18-19 0.9634947938995637 1109 20-21 1.2261822326013174 1109 22-23 0.8075310743149444 1109 24-25 0.38254983507595597 1109 26-27 0.3039517236501226 1109 28-29 -0.712646544079675 1109 30-31 0.8815187862878915 1109 32-33 0.9194955089271843 1109 34-35 0.6203100895598439 1109 36-37 1.4929557482352678 1109 38-39 1.126635961886528 1109 40-41 1.1703027701152635 1109 42-43 1.2763551666348931 1109 44-45 0.9242041079487606 1109 46-47 1.038476457660721 1109 48-49 1.0721222082314057 1109 50-51 0.5310372432175043 1109 52-53 0.7441224372796 1109 54-55 0.03154555184746499 1109 56-57 0.6200252432076567 1109 58-59 0.3601477891794431 1109 60-61 1.0354717189135911 1109 62-63 0.6072483806945428 1109 64-65 1.039055910600105 1109 66-67 0.8337133150800469 1109 68-69 -0.10229732918731571 1109 70-71 -0.06407784894924617 1109 72-73 -0.5603403565837226 1109 74-75 -0.906832960507856 1109 76 0.16752172555741396 1110 1 -0.2153737505379567 1110 2 -0.2036114917661891 1110 3 0.23612239259482237 1110 4 -0.10721345178830433 1110 5 0.47895449486821917 1110 6 0.24132096926017965 1110 7 0.44426347913195485 1110 8 0.7951013592423095 1110 9 1.046440050618088 1110 10-11 -0.45471865839604675 1110 12-13 0.6360383100146052 1110 14-15 0.6431611116989373 1110 16-17 0.6230496764591251 1110 18-19 0.05304157752529193 1110 20-21 0.7861237530691483 1110 22-23 -0.5883022590183842 1110 24-25 -0.18250864445621318 1110 26-27 1.0919634195565564 1110 28-29 -0.21447402945979377 1110 30-31 -0.10751630143141 1110 32-33 1.1040715323189971 1110 34-35 0.8619036568113003 1110 36-37 1.2776779704574892 1110 38-39 0.9497353770912014 1110 40-41 -1.6301358263759624 1110 42-43 0.16012709645945478 1110 44-45 0.8891163886505211 1110 46-47 0.8385495570759218 1110 48-49 1.3133502784068511 1110 50-51 0.17540858824674643 1110 52-53 0.654941150729897 1110 54-55 -0.6117293019537016 1110 56-57 0.3301860619211112 1110 58-59 -0.7546182926919087 1110 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2116 44-45 -0.13531970157504958 2116 46-47 -0.7172775105932487 2116 48-49 0.23402697013617058 2116 50-51 -0.4009072012269357 2116 52-53 0.34253513569230165 2116 54-55 0.009323329625246402 2116 56-57 0.10593794162036119 2116 58-59 -2.1739791949475418 2116 60-61 -0.6815917731499042 2116 62-63 0.7747086981548534 2116 64-65 -1.7363409147967204 2116 66-67 -0.2627152563485282 2116 68-69 -1.1280909799809677 2116 70-71 1.0339380240666216 2116 72-73 0.11882631008294453 2116 74-75 -0.29274565892055193 2116 76 -1.6495417665060756 2117 1 0.6044029639756019 2117 2 0.15364528654322385 2117 3 0.3062978311913156 2117 4 -0.09285938480265798 2117 5 0.3577423736560945 2117 6 0.2967436167880919 2117 7 0.38604976302350025 2117 8 0.5793916303746869 2117 9 -1.0843414485844605 2117 10-11 -0.6273661863067375 2117 12-13 -1.0742886437333965 2117 14-15 0.16708455667500743 2117 16-17 -0.23699817043083016 2117 18-19 -1.0245102566054882 2117 20-21 -0.1803834239643436 2117 22-23 -0.16784771356383743 2117 24-25 -0.8421976396715252 2117 26-27 -1.0457957510973515 2117 28-29 -1.3988839178170593 2117 30-31 0.6201551499242512 2117 32-33 -1.4674994405677637 2117 34-35 1.006042412792162 2117 36-37 -0.089746271966753 2117 38-39 0.5609793962299605 2117 40-41 0.8407573155698032 2117 42-43 0.677870318150049 2117 44-45 0.15147683522148725 2117 46-47 -0.6843770776928153 2117 48-49 -0.7081808220716184 2117 50-51 -0.8130284133481496 2117 52-53 -0.2230492798921091 2117 54-55 0.20326272356463448 2117 56-57 0.6585353442177606 2117 58-59 -0.8241956451639894 2117 60-61 0.0039060623479372225 2117 62-63 0.018864542310701893 2117 64-65 -0.20841883687464957 2117 66-67 0.7920466484133755 2117 68-69 1.0656319637419784 2117 70-71 0.1644575045861032 2117 72-73 1.7748869161435508 2117 74-75 1.6531417869668914 2117 76 1.4617136447493309 2118 1 -0.42590006632742927 2118 2 0.10908200668582424 2118 3 -0.21821197273025206 2118 4 -0.32547970565826745 2118 5 -0.006339622778838816 2118 6 0.2490609073406702 2118 7 0.8490622407418584 2118 8 0.6805502756509796 2118 9 -1.3080491134685985 2118 10-11 -0.8196567388914033 2118 12-13 -1.5346273246603204 2118 14-15 0.49261931603329856 2118 16-17 -3.622693357596603 2118 18-19 -0.3645280819174346 2118 20-21 -0.14963476086273886 2118 22-23 0.5146389174522028 2118 24-25 0.7861446063177837 2118 26-27 -3.157649583539424 2118 28-29 -1.1444275898313165 2118 30-31 -0.6601478803787799 2118 32-33 0.029381129842214193 2118 34-35 0.5120584555729124 2118 36-37 -3.2002632060130978 2118 38-39 -0.8374163256951732 2118 40-41 0.26344002501722485 2118 42-43 0.8989041862427385 2118 44-45 -2.0218743234237877 2118 46-47 -0.08366406521510328 2118 48-49 0.0378084827412124 2118 50-51 -1.2773777894622285 2118 52-53 -1.0826749483413138 2118 54-55 -0.4039119644924014 2118 56-57 0.35572785758674286 2118 58-59 0.17223929244741498 2118 60-61 -0.49965900004065844 2118 62-63 -0.4156274362989194 2118 64-65 0.4352977406654617 2118 66-67 0.8337133150800469 2118 68-69 -1.2583430808213052 2118 70-71 0.5053665954951967 2118 72-73 1.0144145453770577 2118 74-75 -0.3263591042987031 2118 76 -0.26466781692624863 2119 1 0.21926122399515435 2119 2 -0.24860300280184333 2119 3 -0.8818742118194365 2119 4 -0.6632406164742122 2119 5 -0.47265840835758866 2119 6 -0.4857588270046804 2119 7 -0.1941066397135458 2119 8 0.4220113762202402 2119 9 0.08379149374202655 2119 10-11 -0.31040626450809583 2119 12-13 0.3870994305578961 2119 14-15 0.590105084534251 2119 16-17 -0.632892598583318 2119 18-19 0.22939981182070568 2119 20-21 -0.5300901688323734 2119 22-23 0.23831064420742365 2119 24-25 1.1746645304164574 2119 26-27 0.2076255587755682 2119 28-29 -0.602207475979327 2119 30-31 0.3479166357502521 2119 32-33 -0.5090439175961166 2119 34-35 0.2751039963698716 2119 36-37 -0.5973220295425108 2119 38-39 -1.2828622459987784 2119 40-41 -0.4573854019277519 2119 42-43 0.23334441394673178 2119 44-45 0.24140840902403227 2119 46-47 0.3850266368721904 2119 48-49 -0.29615736166106643 2119 50-51 0.25393150845048496 2119 52-53 -0.047096200713227176 2119 54-55 0.36577494252847487 2119 56-57 -2.1691772657529143 2119 58-59 -0.92927873411805 2119 60-61 -0.4156931556383796 2119 62-63 -0.14095950461012308 2119 64-65 0.4602028640051188 2119 66-67 0.6038746054026234 2119 68-69 1.4617707711711105 2119 70-71 0.9005278858177732 2119 72-73 1.0236650197603652 2119 74-75 0.6968856774849712 2119 76 0.6840987864893151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 1.0 22 2.0 23 6.0 24 19.0 25 24.0 26 56.0 27 92.0 28 165.0 29 274.0 30 416.0 31 695.0 32 1035.0 33 1632.0 34 2476.0 35 3693.0 36 5920.0 37 10200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.61914033248465 23.726973191553093 11.157705556387599 31.496180919574655 2 15.45978732963906 18.870750336977686 42.661374868953125 23.008087464430133 3 15.613299385951777 25.41186161449753 33.93739703459638 25.037441964954322 4 11.689381458738955 23.408716489441368 38.46038640107833 26.441515650741348 5 10.9592631421297 38.19454844990265 38.898457391043884 11.947731016923768 6 24.760371424292344 41.77400029953572 17.882282462183614 15.583345813988316 7 23.66706604762618 30.983225999700466 23.262692826119515 22.08701512655384 8 20.80649992511607 39.403923917927216 20.8102441216115 18.979332035345216 9 21.13224502021866 15.504717687584243 22.079526733562975 41.28351055863412 10-11 23.133518047027106 27.09300584094653 28.721731316459486 21.051744795566872 12-13 22.50823723228995 21.867979631571068 27.707054066197394 27.916729069941592 14-15 21.124756627227796 23.698891717837352 22.794668264190506 32.38168339074435 16-17 23.867380560131796 27.793170585592332 23.590310019469822 24.74913883480605 18-19 23.99842743747192 26.216863861015426 24.44211472218062 25.342593979332033 20-21 21.21274524487045 25.72824621836154 24.11636962707803 28.942638909689983 22-23 21.73318855773551 28.908941141231093 24.853976336678148 24.50389396435525 24-25 23.455518945634264 29.715815485996703 24.76785981728321 22.060805751085816 26-27 22.809645050172232 30.157630672457692 25.587838849782834 21.44488542758724 28-29 20.203684289351507 25.629025011232585 29.64280365433578 24.524487045080125 30-31 20.823348809345514 28.13763666317208 27.089261644451103 23.9497528830313 32-33 19.919125355698668 27.881159203234983 30.31301482701812 21.886700614048223 34-35 22.75348210274075 28.880859667515352 27.926089561180174 20.439568668563727 36-37 21.08544256402576 30.483375767560283 26.03527033098697 22.395911337426988 38-39 20.840197693574957 26.21873595926314 28.09083420697918 24.85023214018272 40-41 19.92661374868953 24.98689531226599 29.730792271978434 25.355698667066047 42-43 23.74756627227797 23.74194997753482 30.51707353601917 21.993410214168037 44-45 19.35000748839299 26.7504118616145 29.502396285757076 24.397184364235436 46-47 22.667365583345813 23.839299086416055 29.069941590534672 24.423393739703457 48-49 21.604013778643104 23.59592631421297 28.487719035494983 26.312340871648942 50-51 19.067320652987867 24.028381009435375 31.013179571663922 25.891118765912836 52-53 18.55623783136139 24.202486146472967 34.38482851580051 22.856447506365136 54-55 17.14654785083121 25.101093305376665 31.80320503220009 25.949153811592033 56-57 21.14347760970496 24.704208476860863 28.54949827766961 25.602815635764564 58-59 18.013329339523736 26.364759622584995 31.87996106035645 23.74194997753482 60-61 20.302905496480456 29.3301632469672 26.55009734910888 23.816833907443463 62-63 17.101617492886025 33.360790774299836 25.707653137636665 23.829938595177474 64-65 17.528455893365283 33.51055863411712 24.241800209675006 24.719185262842593 66-67 17.51160700913584 33.72584993260446 23.717612700314515 25.044930357945184 68-69 17.708177325146025 33.09120862662873 23.953497079526734 25.247116968698517 70-71 18.27167889770855 31.89680994458589 23.871124756627225 25.960386401078328 72-73 17.949677999101393 30.59757376067096 23.99281114272877 27.459937097498877 74-75 18.076980679946082 29.968548749438373 24.65927811891568 27.29519245169987 76 17.575258349558183 30.81848135390145 24.809045978732964 26.7972143178074 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 3.5 21 6.0 22 6.0 23 12.0 24 18.0 25 18.0 26 39.5 27 77.0 28 121.0 29 149.0 30 163.0 31 200.0 32 297.5 33 372.0 34 439.0 35 571.5 36 705.0 37 773.0 38 1064.0 39 1595.0 40 1924.5 41 2050.5 42 2087.0 43 2044.0 44 1956.0 45 1972.5 46 2034.0 47 2021.0 48 1864.0 49 1623.0 50 1526.0 51 1348.0 52 1053.5 53 873.0 54 809.0 55 740.5 56 578.0 57 425.0 58 366.0 59 300.5 60 202.5 61 152.0 62 134.0 63 103.0 64 59.0 65 41.0 66 26.0 67 16.0 68 17.0 69 11.5 70 4.5 71 4.0 72 2.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 26708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.51699865208926 #Duplication Level Percentage of deduplicated Percentage of total 1 62.321361058601134 30.85966751535121 2 17.822306238185256 17.650142279466827 3 8.74102079395085 12.984873446158455 4 4.3478260869565215 8.611651939493784 5 2.608695652173913 6.458738954620339 6 1.5425330812854443 4.582896510408866 7 0.9300567107750473 3.2237531825670214 8 0.46880907372400754 1.8571214617343117 9 0.3629489603024575 1.6174928860266586 >10 0.8166351606805292 5.447805900853677 >50 0.015122873345935726 0.5953272427737009 >100 0.015122873345935726 2.182866556836903 >500 0.0 0.0 >1k 0.007561436672967863 3.9276621237082523 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT 1049 3.9276621237082523 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT 454 1.6998652089261643 TruSeq Adapter, Index 12 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC 129 0.48300134791073834 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG 85 0.31825670211172685 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 74 0.2770705406619739 No Hit CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC 48 0.17972143178073985 RNA PCR Primer, Index 12 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT 39 0.14602366332185113 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT 35 0.1310468773401228 RNA PCR Primer, Index 12 (95% over 22bp) GTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGT 27 0.10109330537666616 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.018720982477160402 0.0 5 0.0 0.0 0.0 0.029953571963456643 0.0 6 0.0 0.0 0.0 0.029953571963456643 0.0 7 0.0 0.0 0.0 0.029953571963456643 0.0 8 0.0 0.0 0.0 0.037441964954320804 0.0 9 0.0 0.0 0.0 0.10109330537666616 0.0 10 0.0 0.0 0.0 0.1123258948629624 0.0 11 0.0 0.0 0.0 0.1310468773401228 0.0 12 0.0 0.0 0.0 0.1310468773401228 0.0 13 0.0 0.0 0.0 0.13853527033098698 0.0 14 0.0 0.0 0.0 0.15351205631271528 0.0 15 0.0 0.0 0.0 0.15351205631271528 0.0 16 0.0 0.0 0.0 0.1684888422944436 0.0 17 0.0 0.0 0.0 0.17972143178073985 0.0 18 0.0 0.0 0.0 0.17972143178073985 0.0 19 0.0 0.0 0.0 0.2096750037441965 0.0 20 0.0 0.0 0.0 0.22090759323049275 0.0 21 0.0 0.0 0.0 0.232140182716789 0.0 22 0.0 0.0 0.0 0.25460536168938147 0.0 23 0.0 0.0 0.0 0.27332634416654183 0.0 24 0.0 0.0 0.0 0.27707054066197395 0.0 25 0.0 0.0 0.0 0.27707054066197395 0.0 26 0.0 0.0 0.0 0.2957915231391343 0.0 27 0.0 0.0 0.0 0.325745095102591 0.0 28 0.0 0.0 0.0 0.3594428635614797 0.0 29 0.0 0.0 0.0 0.43058259697468926 0.0 30 0.0 0.0 0.0 0.46802456192901004 0.0 31 0.0 0.0 0.0 0.5241875093604912 0.0 32 0.0 0.0 0.0 0.6028156357645649 0.0 33 0.0 0.0 0.0 0.703908941141231 0.0 34 0.0 0.0 0.0 0.8050022465178973 0.0 35 0.0 0.0 0.0 0.9622584993260447 0.0 36 0.0 0.0 0.0 1.1831660925565373 0.0 37 0.0 0.0 0.0 1.5538415456043133 0.0 38 0.0 0.0 0.0 2.006889321551595 0.0 39 0.0 0.0 0.0 2.5872397783435677 0.0 40 0.0 0.0 0.0 3.3398232739254157 0.0 41 0.0 0.0 0.0 4.197244271379362 0.0 42 0.0 0.0 0.0 5.152014377714543 0.0 43 0.0 0.0 0.0 6.0056911786730565 0.0 44 0.0 0.0 0.0 6.833158604163547 0.0 45 0.0 0.0 0.0 7.709300584094653 0.0 46 0.0 0.0 0.0 8.750187209824771 0.0 47 0.0 0.0 0.0 9.645050172233038 0.0 48 0.0 0.0 0.0 10.670960011981428 0.0 49 0.0 0.0 0.0 11.46472966901303 0.0 50 0.0 0.0 0.0 12.251010933053767 0.0 51 0.0 0.0 0.0 13.041036393589936 0.0 52 0.0 0.0 0.0 13.82357346113524 0.0 53 0.0 0.0 0.0 14.5424591882582 0.0 54 0.0 0.0 0.0 15.253856522390295 0.0 55 0.0 0.0 0.0 15.939044481054365 0.0 56 0.0 0.0 0.0 16.583046278268682 0.0 57 0.0 0.0 0.0 17.31316459487794 0.0 58 0.0 0.0 0.0 17.987119964055715 0.0 59 0.0 0.0 0.0 18.66481952972892 0.0 60 0.0 0.0 0.0 19.387449453347312 0.0 61 0.0 0.0 0.0 19.937846338175827 0.0 62 0.0 0.0 0.0 20.54066197394039 0.0 63 0.0 0.0 0.0 21.083570465778045 0.0 64 0.0 0.0 0.0 21.787479406919275 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGCACA 15 0.0022011145 70.0 17 ACGTCTC 15 0.0022011145 70.0 58 AATTTAC 15 0.0022011145 70.0 53 TATCGTA 15 0.0022011145 70.0 38 ATGGCAC 15 0.0022011145 70.0 16 TCTCGAG 15 0.0022011145 70.0 61 GTCTCGA 15 0.0022011145 70.0 60 CGAGTGA 15 0.0022011145 70.0 64 GTGATGT 15 0.0022011145 70.0 67 AATATCG 15 0.0022011145 70.0 36 ATCGTAT 20 7.787866E-5 70.0 39 ATATCGT 15 0.0022011145 70.0 37 TCGAGTG 15 0.0022011145 70.0 63 ATTCAGC 20 7.787866E-5 70.0 43 CGTCTCG 15 0.0022011145 70.0 59 CTCGAGT 15 0.0022011145 70.0 62 CAGCCAT 25 2.3488431E-4 56.000004 46 TGATTCA 25 2.3488431E-4 56.000004 41 AAGGTAG 25 2.3488431E-4 56.000004 30 TTCAGCC 25 2.3488431E-4 56.000004 44 >>END_MODULE