FastQCFastQC Report
Wed 25 May 2016
SRR1780621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTGGGACTGCCCGTGTTCGTCCTGCCCGCCCTGCGCCTGGGCGTGTGG11.0869565217391304No Hit
GCCCGGGCCCCTCCCTGGGGGGGGCCGGGCAGGGGGCCGCGCCGGAAACA11.0869565217391304No Hit
GAAAGGGACGCGGGCGGGGATATAGGGTCGAAGCCGCACTCGTAAGGGGT11.0869565217391304No Hit
GATTCAAACCTGCCGGGGCTTCTACCGCCTTTTTTCCCGGAGGCGGGGAA11.0869565217391304No Hit
CACCCGCTGATGCGTGGCAGCATAGGTAGTGTGGTCCACTGGCTTGCTGG11.0869565217391304No Hit
TTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTTGATTAGTCA11.0869565217391304No Hit
GCTTTGAGGAGGTAAGCTACATAAACTGTGGGGGGTGTCTTTGGGGTTTG11.0869565217391304No Hit
TGTCTAACAACATGGCTTTCTCAACTTTTAAAGGATAACAGCTATCCATT11.0869565217391304No Hit
ACCTACCGCTCCGTGCATGCCGATGCCCTCACCGAGCGGGACGTGGAGCC11.0869565217391304No Hit
ACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCA11.0869565217391304No Hit
CGCCAAGCTCTCCTGCTGCGCGGCGTGAACCTGCCCCGCACACAGGGACA11.0869565217391304No Hit
GGCGGCAGGAAGCGTTCAGAGGAACGGCCGCCGTCGTAGGAGCTGTCTCT11.0869565217391304No Hit
GTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTTGATT11.0869565217391304No Hit
CGCGCGGACCGTCCAGTGCAGGTGGCGGAGGAAGTCGGCGGTGCCGCGGC11.0869565217391304No Hit
TTATAACTATTCAGCCCATTAAGGATTTATTTTGTTGAGTATTATGAGAA11.0869565217391304No Hit
ACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAG11.0869565217391304No Hit
GTATTGGGTTATGGCAGGGGGGTTTTTATTGATAATTGTTGTGATGAAAT11.0869565217391304No Hit
GTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAGGGAGTATA11.0869565217391304No Hit
GGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTAT11.0869565217391304No Hit
AGAGCCCCTCAGTGCAGAAAGTTGTAAAGGTGGCAGCACGGTGGCTCATG11.0869565217391304No Hit
GCCCGGGGTGGAGTCCGATCCGGCCGCGCCCCAGGGCGGCGGCCTGCGCG11.0869565217391304No Hit
CGCCGGATGTGGTGCTGCTGGACGTGCACCTGCCCGGCGGGGCCGGCGGC11.0869565217391304No Hit
CCCTAGGGCTGAGGGCTGCGATGGAGCACGCAAGTATTCTCTTCCCCCCC11.0869565217391304No Hit
CGCTTAACGTGTCCGCCTCCCGCGCGAAGATCGAGTGGTCGGAAGCCTCG11.0869565217391304No Hit
CTTATACACATCTGACGCTGCCGACGACGACGCCCGTGGCTATCTCTGTG11.0869565217391304No Hit
GGTAGAGGGGGCGCTATAGGGCAAATACGGGCCCTATTTCAAAGATTTTT11.0869565217391304No Hit
ACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGGAGGCACCA11.0869565217391304No Hit
TCCCGGCAGCGCTCCGAGGACGAGGACGCCGGAGAGTCCGAGGACCGGGC11.0869565217391304No Hit
AAAGGGACAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCT11.0869565217391304No Hit
CGAGCGACCTGGGTCATCGATCAGACGGCGGCCGAAGCCCCTGGCCACCA11.0869565217391304No Hit
GTGCCGGACTGACCCCCAGACCTTGTCGACCGCCCCGGCCCCGTGCCTAG11.0869565217391304No Hit
TCTCTGGTGCGGGCTGCTCGCTCGCCGCCGCCGTCACCGCCGAGTTGGCC11.0869565217391304No Hit
CCCTCGACCATCCGCGCCGTGGACGTCTTGCCGTTGGTGCCGGTCAGGCG11.0869565217391304No Hit
GGCGAGATCCTCGTCTTCCTCGACGTGGACTGCATCCCCTGTCTCTTATA11.0869565217391304No Hit
GCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCAC11.0869565217391304No Hit
CCCGATCGGGACCCGGGCAACCAGTGTGACGCGTGCGTGGCCCCGCCACC11.0869565217391304No Hit
GGCCGTGGAGGTGCGCCGCCGTCTCGTGGGAGAGAACGGCGTCGGGACGG11.0869565217391304No Hit
CGTTCCACAGAAGCTGCCATCAAGTATTTCCTCACGCAAGCAACCGCATC11.0869565217391304No Hit
CCATCATAGCCACCATCACCCTCCTTAACCTCTCCTTCTACCTGTCTCTT11.0869565217391304No Hit
GGGCGCAACCCCCCACGCGGGGTCAAGACGCCGAGCCGCGCTCCCTGCCG11.0869565217391304No Hit
CGAGGGGCAGCTGAAGCCCTCGGCGCGGAGCAGGGCTGTCTCTTATACAC11.0869565217391304No Hit
CCGTGAAGTCCGAGGGGTGGACGCCGTGGGTGTCGAAGTGCTCGCGGTAC11.0869565217391304No Hit
CGCCCCCACGGCCCCGCGCGCCCAGGACTGGCTCTGTCTCTTATACACAT11.0869565217391304No Hit
CCCGTGTGGCCGCGCTCGCCCGCCACCTGGAGGCCGAGGGCTGTCTCTTA11.0869565217391304No Hit
TTCTTCGACCCCGCCGGAGGAGGAGACCCCATTCTATACCAACACCTATT11.0869565217391304No Hit
CCCTAGCACCATCCTAACCATATTTCAAATTTTATCTTTTGGCGTTATGC11.0869565217391304No Hit
CTCCTGGGTGGTGGGGCACTGTGCCCGCTGCCCGCTAGGTGTCGCTGTCC11.0869565217391304No Hit
CCGCTACACCTCCCCCGGCGCCGCCCCCGCAACCCCTGCCGCTCTCGGGG11.0869565217391304No Hit
GCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTATGT11.0869565217391304No Hit
ACTTTACAGTCAGAGGTTCACTTCCTCTTCTTAACAACAAACCCATGGCC11.0869565217391304No Hit
CGCCGCGCGGAACTGACGCGCGGGCCCGGCGCGTCGTCGCCGGGACTAGG11.0869565217391304No Hit
TCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTA11.0869565217391304No Hit
GTGCGGGGAGCTGGTTATCAGAGGCCGGGGTGGCGCGGGCCGGTTTTGTA11.0869565217391304No Hit
CGTAGGGCCGGTCCGCCGCGTGCAGGACCGGCTGCGCGGCGGCGAGCTCC11.0869565217391304No Hit
CGGCCAGGCCGAAGGGCGTCGGGAACCGCAGGCCCATGACCGTGCGCCCG11.0869565217391304No Hit
CGGTGAGCACGAGCCCGAGCACGCGCGCCACCATGAGCTGTCTCTTATAC11.0869565217391304No Hit
GGACCATCCCCACGACGCGAAGGACCCCGTCATGCCTGTCTCTTATACAC11.0869565217391304No Hit
CATGAATGCTGTGCAATGGTTTTTTTTTTTTTTTTTTGTCCCTCTTTTAA11.0869565217391304No Hit
CCCTAGCATTACTTATATGACATGTCTCCATACCCATTACAATCTCCAGC11.0869565217391304No Hit
CGCTACCATAATCATCGCTATCCCCACCGGCGTCAAAGCTGTCTCTTATA11.0869565217391304No Hit
GGCTTCTCCAACTCGGGCTTCGAGATCCTGGCCGGCATCGGCGTGTTCTC11.0869565217391304No Hit
GCGTTTGGTATTGGGTTATGGCAGGGGGGTTTATATTGATAATTGTTGTG11.0869565217391304No Hit
TGTGGGTGGGTGGTTGATTGGGAGGTCTTGGTCGGCCTCATTAAGTTTTT11.0869565217391304No Hit
CTCGCCACACTCCACGGAAGCAATATGCACTGCTCTGCTGCAGTGCTCTG11.0869565217391304No Hit
ACTGTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGA11.0869565217391304No Hit
CGCGAACTCCGCGTACACGTCGCGGGCGGCGGTGCCGTCGATCGAGTTCC11.0869565217391304No Hit
ATGCTTCACTCAGCCATTTTACCTCACCCCCACTGATGTTCGCCGACCGT11.0869565217391304No Hit
ATGTTAGCTTGTTTCAGGTGCGAGATAGTAGTAGGGTCGTGGTGCTGGAG11.0869565217391304No Hit
AGGGAGAGGGGGATTGTTGTTGGGAGGGGGGATGGGGGGGAAGTGTTGGG11.0869565217391304No Hit
AGTATAGAGAGGTAGAGTTTTTTTCGTGATAGTGGTTCACTGGATAAGTG11.0869565217391304No Hit
GAGTAGTCGGGCTAGGCCCACCGGTGCGGCGCAGGCGGCACGGTCTAGTC11.0869565217391304No Hit
ACACAAAATGACATCAAAAAAATCGTAGCCTTCTCCACTTCAAGTCAACT11.0869565217391304No Hit
ACATATTCCAAAGGCAACGGAGAAAACTTGGACCCCTGGCTCCGGTTCCG11.0869565217391304No Hit
CTGCACGAAGGACGCGCCCGCGGACTCCTCGCCGCCGAACACGCCGGTGC11.0869565217391304No Hit
ATATTATACTAGCATTTACCATCTCACTTCTAGGAATACTAGTACTGTCT11.0869565217391304No Hit
GCCCCCGCCCCGCCCACCGCAGAGCTCGCCGCCGGGGAGGCCCCCCAGTG11.0869565217391304No Hit
CTAGTCACAGCCCTATACTCCCTCTACATATTTACCACAACACAATGGGG11.0869565217391304No Hit
GTCGTCACCGCGCAGACGCCGGACAACCTCAAGGACCCCGCCATCCACCG11.0869565217391304No Hit
GGTGCTGTGCACACAAGGCCTGTGATAACTAGTGATAAGTGTTACTGGAG11.0869565217391304No Hit
GTGTAAGCCGTACCGAGAGGAACGGTAAGAATCATGACCATGATCCTGTC11.0869565217391304No Hit
GTTAACCCCTGTCCACATAGACAGTCTTTGGGTACTCAGGAAGCTGCCAT11.0869565217391304No Hit
AAATAGGAGAAGGCTTAGAAGAAAACCCCACAAACCCCATTACTAAACCC11.0869565217391304No Hit
CTTATACACATCTGACGCTGCCGAGAACTTCTAGGTGTAGATCTTGGTGG11.0869565217391304No Hit
CTTCTGCCCGACGACGGCGGCGCCGCCGGCGATGCCGACCTCGATGCCCG11.0869565217391304No Hit
GTCTTTGGATATACTACAGCGATGGCTATTGAGGAGGATACTGAGGCCTG11.0869565217391304No Hit
CCAGCATCTCCTCCCTCGGCATCCGAGGACCCCAATTATAGCAACCCCGC11.0869565217391304No Hit
TCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTA11.0869565217391304No Hit
CTTATACACATCGACGCTGCCGACGAATCTGGCGGTGTAGATCTCGGTGG11.0869565217391304No Hit
TCGCAGGACCGCACGGAAGGACCCGTCGCATGAGCGTGTCGACCTGTCTC11.0869565217391304No Hit
GGCCAGGCCCCGGGCGGCCAGGGCGACGTCGGCGCGTGCGGTCTCAGCCA11.0869565217391304No Hit
TCATCAGGGGTGCCTGACGAGGGAGGTAGATACGCGGATGAATTGGGTGG11.0869565217391304No Hit
TTATGGTACCGTGCAATATTCATGGTGGCTGGCAGTAATGTACGAAATAC11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATC50.044.037
CGCTGCC50.044.016
GAGAACT50.044.023
ACGCTGC50.044.015
ATCTTGG50.044.041
CACATCT50.044.07
TCTAGGT50.044.030
TATACAC50.044.03
GTGTAGA50.044.035
TGCCGAG50.044.019
CATCTGA50.044.09
CTGACGC50.044.012
ACATCTG50.044.08
AGAACTT50.044.024
CTAGGTG50.044.031
TTATACA50.044.02
ACTTCTA50.044.027
AGGTGTA50.044.033
TACACAT50.044.05
ACACATC50.044.06
Produced by FastQC (version 0.11.5)