FastQCFastQC Report
Wed 25 May 2016
SRR1780621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATATCCGAGCCCACGAGACGGCGTCGAATCTCGTATGCCGT11.0869565217391304No Hit
CATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCT11.0869565217391304Illumina PCR Primer Index 3 (96% over 26bp)
GGTCCGTGCGCACCTGCCCGCTCTGCTCGCCGGCGGGGCCGAGGCCTTCC11.0869565217391304No Hit
GCGCCGCGCAGGCCGCCGCCCTGGGGCGCGGCCGGATCGGACTCCACCCC11.0869565217391304No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT11.0869565217391304No Hit
CCTCCGCTGGACCCTGCACCACGCCTACGAGAACGTGCCCGCGTACCGCG11.0869565217391304No Hit
CATCTGTGTCATGCCTCACCGAGGCTTCCGACCACTCGATCTTCGCGCGG11.0869565217391304No Hit
CTACTGACTATCCTAGAAATCGCTGTCGCCTTAATCCGAGCCCACGAGAC11.0869565217391304No Hit
ATGATGTATGCTTTGTTTCTGTTGAGTGTGGGTTTAGTAATGGGGTTTGT11.0869565217391304No Hit
ATACACATCTCCGACCCACGAGACCTCATGGGATCTCGTATGCCGTCTTC11.0869565217391304RNA PCR Primer, Index 34 (95% over 24bp)
CCCTCACCCACTAGGATACCAACAAACCTACCCATCTTTAACAGTACATA11.0869565217391304No Hit
TCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTTCTGCTTGAA11.0869565217391304TruSeq Adapter, Index 25 (96% over 29bp)
GGATCGACTGGGTCGTCCGGGACGAGGACGAGAACTGGCGGATCACCACC11.0869565217391304No Hit
CCGCCGCCCTGCGCGCCGCCTTCCGCCCGGACCCGCTGCTCGGGCGCACG11.0869565217391304No Hit
GCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAAT11.0869565217391304No Hit
ATGAAGCCCAGGGCGGAGAACACGCCGATGCCGGCCAGGATCTCGAAGCC11.0869565217391304No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC11.0869565217391304No Hit
GTGGTCCTCGGCGCGACGGCACCACGCCTGCAGGTAGAGGCGCGCCCCGT11.0869565217391304No Hit
ACCTAGGCACGGGGCCGGGGCGGTCGACAAGGTCTGGGGGTCAGTCCGGC11.0869565217391304No Hit
GATTATAGGCATGAGCCACCGTGCTGCCACCTTTACAACTTTCTGCACTG11.0869565217391304No Hit
CCCTCGGCCTCCAGGTGGCGGGCGAGCGCGGCCACACGGGCTGTCTCTTA11.0869565217391304No Hit
CTACCGTACAACCCTAACATAACCATTCTTAATTTAACTATTTATATTAT11.0869565217391304No Hit
GATTACGTACTCCACCTTGACTCATTCCAATTCATTCTTCACCCTGACTC11.0869565217391304No Hit
CTTATACACATCTCCGAGCCCACGAGCGATCCAAAATCTCGTATGCCGTC11.0869565217391304No Hit
GCATGACGGGGTCCTTCGCGTCGTGGGGATGGTCCCTGTCTCTTATACAC11.0869565217391304No Hit
CATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTTTTTCT11.0869565217391304Illumina PCR Primer Index 3 (95% over 22bp)
CGCCGCGGCACCACCGACATCCTCGGCCCCCTGGACTGGACGGTCCGCGC11.0869565217391304No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTGCTATTCG11.0869565217391304No Hit
CTTGGCCAACTCGGCGGTGACGGCGGCGGGGGGCGAGCAGCCCGCCCCCG11.0869565217391304No Hit
CATCTCCGACCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTT11.0869565217391304Illumina PCR Primer Index 10 (96% over 27bp)
TCTTTATACACATCTCAGAGCCCACGAGACCTCATGGGATCTCGTATGCC11.0869565217391304No Hit
GCCGGTCACTGGGGGGCCTCCTCGGCGGCGAGCTCGGCGGCTGGAGGGGC11.0869565217391304No Hit
ATTAGAAGGATTATGGATGCGGTTGCTTGCGTGAGGAAATACTTGATGGC11.0869565217391304No Hit
CACCTCCGCTCCCCCGCCGCCGGCCCCGCCGGGCAGGTGCACGTCCAGCA11.0869565217391304No Hit
TACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTACAATA11.0869565217391304No Hit
ACACTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCT11.0869565217391304Illumina PCR Primer Index 1 (96% over 25bp)
CTCATGGTGGCGCGCGTGCTCGGGCTCGTGCTCACCGCTGTCTCTTATAC11.0869565217391304No Hit
CACTAGGATACCAACAAACCTACCCATCTTTAACAGTACATAGTACATAA11.0869565217391304No Hit
TGGGGGCATGAGTTAGCAGTTCTTGTGAGCTTTCTCGGTAAATAAGGGGT11.0869565217391304No Hit
GATATGAGGTGTGAGCGATATACTAGTATTCCTAGAAGTGAGATGGTAAA11.0869565217391304No Hit
ATCGGTGTCCCTGTGTGCGGGGCAGGTTCACGCCGCGCAGCAGGAGAGCT11.0869565217391304No Hit
AGTTTCTTCTCATAATACTCAACAAAATAAATCCTTAATGGGCTGAATAG11.0869565217391304No Hit
GCACCTAATCCAAACCAGTTTAAGGGTGGAAAGGAAATGTATTGGCTCTG11.0869565217391304No Hit
ATCCTAGACCCGGCGACGACGCGCCGGGCCCGCGCGTCAGACCCGCGCGG11.0869565217391304No Hit
ATGAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTA11.0869565217391304No Hit
TACTAGTATTCCTAGAAGTGAGATGGTAAATGCTAGTATAATATCTGTCT11.0869565217391304No Hit
GTTTCATGCCCATCGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATA11.0869565217391304No Hit
CCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA11.0869565217391304Illumina PCR Primer Index 1 (96% over 27bp)
CCGACGAGGTCGGCGAGGCCAATTCGGCGGCCATGTCGGACTCCCAGCAG11.0869565217391304No Hit
CACACACACCGCTGCTAACCCCATACCCCGAACCAACCAAACCCCAAAGA11.0869565217391304No Hit
GTGTGGAACTCGATCGACGGCACCGCCGCCCGCGACGTGTACGCGGAGTT11.0869565217391304No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC11.0869565217391304No Hit
CTTTACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTT11.0869565217391304No Hit
GACAGGACTTGGCCTTTGCTTAATAAAATAACTGGCTGCTCTGTAGAGAA11.0869565217391304No Hit
CGTCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGA11.0869565217391304Illumina PCR Primer Index 1 (96% over 27bp)
TCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTTG11.0869565217391304TruSeq Adapter, Index 10 (96% over 28bp)
GTGCCGGGACTGCTGACGGGCACCGGCGTGTTCGGCGGCGAGGAGTCCGC11.0869565217391304No Hit
CAACTACCTAACCAACAAACTTAAAATAAAATCCCCACTATGCACATTTT11.0869565217391304No Hit
GACCAATGGATAGCTGTTATCCTTTAAAAGTTGAGAAAGCCATGTTGTTA11.0869565217391304No Hit
CTCATCATCCCCACCATCATAGCCACCATCACCCTCCTTAACCTCTACTT11.0869565217391304No Hit
GCCACGATCAGCACCCACCACGGCACGGTGGGCAGCCCGGGCGCGAGCCC11.0869565217391304No Hit
ACCCAGTCCTGCGCGCGCGGGGCCGTGGGGGCGCTGTCTCTTATACACAT11.0869565217391304No Hit
GGGATGCAGTCCACGTCGAGGAAGACGAGGATCTCGCCCTGTCTCTTATA11.0869565217391304No Hit
CTTTGACGCCGGTGGGGATAGCGATGATTATGGTAGCGCTGTCTCTTATA11.0869565217391304No Hit
GGCCCCTCCTCCGCAGCTCCAGCACCCTAGGGCCTGAGGAGGCGGGAGAC11.0869565217391304No Hit
CTCCTACGACGGCGGCCGTTCCTCTGAACGCTTCCTGCCGCCCTGTCTCT11.0869565217391304No Hit
ATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTT11.0869565217391304No Hit
GAACCCAACCTCCGAGCCCACGAGACTTCGCGGCATCTCTTATGCCGGCC11.0869565217391304No Hit
GTCGACACGCTCATGCGACGGGTCCTTCCGTGCGGTCCTGCGACTGTCTC11.0869565217391304No Hit
AGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTTCTGCTTGAAAAAA11.0869565217391304RNA PCR Primer, Index 36 (96% over 28bp)
GGCCCTGGACGGTGAGCTCGCCGTCGGCGGCGCGGTCCACGCCCTGTCTC11.0869565217391304No Hit
CCCTCGGCCCGGTCCTCGGACTCTCCGGCGTCCTCGTCCTCGGAGCGCTG11.0869565217391304No Hit
GCCCTGGGAGGGTGAGGCCTCCCAATCCCTCTGCAACACCCAGAAACCAG11.0869565217391304No Hit
CTATACACATTTCCGAGCCCACGAGACTTCGCGGCATCTTTGAGGCCGTC11.0869565217391304No Hit
GCCCCCGTGATCCACCTGACCGGCACCAACGGCAAGACGTCCACGGCGCG11.0869565217391304No Hit
ATCACACACCGCACAATCCCCTATCTAGGCCTTCTCCGAGCCCACGAGAC11.0869565217391304No Hit
AAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTA11.0869565217391304No Hit
ATCCAACATCTCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT11.0869565217391304RNA PCR Primer, Index 27 (95% over 22bp)
AACGGCGCGGCCGTGGCCCTCATGGTGATCTCGTTCGCCTCCACCGGCGG11.0869565217391304No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT11.0869565217391304No Hit
GCCTTGTTCCAGATGTCGATCAGCTCGGAGCGACGCTCGTCGTCCGCGAT11.0869565217391304No Hit
GAGCAAGATTCTTTAGATCGGGGAGGTAGGTCAAATGGGAAACTGGTTTC11.0869565217391304No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT11.0869565217391304TruSeq Adapter, Index 7 (95% over 21bp)
ATACACAGCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT11.0869565217391304TruSeq Adapter, Index 7 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC11.0869565217391304No Hit
CCCTGCTCCGCGCCGAGGGCTTCAGCTGCCCCTCGCTGTCTCTTATACAC11.0869565217391304No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC11.0869565217391304No Hit
GACAAGTAGCATCCGTACTATACTTCACAACAATCCTAATCCTAATACCA11.0869565217391304No Hit
GCACCCCCTCTACCCCCTCCGAGCCCACGAGACTTCGCGGCATCACTAAT11.0869565217391304No Hit
CTCCTGCGGTCCCGTCCCGACGCCGTTCTCTCCCACGAGACGGCGGCGCA11.0869565217391304No Hit
TTCGAATATCTTGTTCATTGTTAAGGTTGTGGATGATGGATCCGGAGCAC11.0869565217391304No Hit
TAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATT11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAT50.044.018
AGCCCAC50.044.05
CATGATG50.044.016
GTATGCC50.044.030
ATCTCGT50.044.025
GCCCACG50.044.06
ACATGAT50.044.015
TGCCGTC50.044.033
GATATCT50.044.022
ATGATGA50.044.017
ATGATAT50.044.020
CCACGAG50.044.08
TATGCCG50.044.031
TTCTGCT50.044.040
CCGTCTT50.044.035
GAGCCCA50.044.04
CACGAGA50.044.09
CCCACGA50.044.07
TGATATC50.044.021
GATGATA50.044.019