FastQCFastQC Report
Wed 25 May 2016
SRR1780620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106636
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAAGT150.002220240769.99296616
AAGTCAA252.3823585E-455.99437364
CTAGATA200.00693759252.49472443
TCCATGT200.00693759252.49472430
ACGCCTT200.00693759252.49472410
GATGTAT200.00693759252.49472424
CGTGCGA200.00693759252.49472410
CGCGAGA200.00693759252.49472432
AGCGGTC200.00693759252.4947247
GAGCGGT200.00693759252.4947246
TCTAATT200.00693759252.49472437
GGTATTC200.00693759252.4947243
TGGATGT200.00693759252.49472436
CGATGGC200.00693759252.49472414
CAGAGCG200.00693759252.4947244
GGCTCGC200.00693759252.49472428
AGAGCGG200.00693759252.4947245
CAAGTCA305.8582E-446.66197663
CCTCGGA451.6724916E-646.66197645
CTGCGCC350.001251269739.9959813
Produced by FastQC (version 0.11.5)