##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780620_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 106636 Sequences flagged as poor quality 0 Sequence length 76 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.1188247871263 34.0 31.0 34.0 12.0 34.0 2 31.592473461120072 34.0 33.0 34.0 23.0 34.0 3 31.670674068794778 34.0 34.0 34.0 23.0 34.0 4 32.0684290483514 34.0 34.0 34.0 27.0 34.0 5 32.042584117933906 34.0 34.0 34.0 27.0 34.0 6 34.71746877227203 38.0 36.0 38.0 26.0 38.0 7 34.24951235980345 38.0 35.0 38.0 24.0 38.0 8 33.06314002775798 37.0 34.0 38.0 21.0 38.0 9 33.85371731872913 37.0 34.0 38.0 22.0 38.0 10-11 34.30003938632357 37.5 34.5 38.0 24.5 38.0 12-13 34.672291721369895 38.0 35.5 38.0 25.5 38.0 14-15 34.27588713004989 38.0 34.5 38.0 23.5 38.0 16-17 34.127442889830824 38.0 34.0 38.0 23.0 38.0 18-19 34.11629280918264 37.5 34.0 38.0 23.0 38.0 20-21 34.18760549908099 38.0 34.0 38.0 23.5 38.0 22-23 34.199458906935746 37.5 34.5 38.0 23.5 38.0 24-25 34.59645898195731 38.0 35.5 38.0 25.0 38.0 26-27 34.66819835702765 38.0 36.0 38.0 25.5 38.0 28-29 34.81401215349413 38.0 36.0 38.0 26.5 38.0 30-31 34.64431336509246 38.0 36.0 38.0 26.0 38.0 32-33 34.393647548670245 38.0 35.0 38.0 25.0 38.0 34-35 34.22024925916201 37.5 34.0 38.0 24.0 38.0 36-37 34.22298285757155 37.5 34.5 38.0 23.5 38.0 38-39 34.76828181852282 38.0 36.0 38.0 26.0 38.0 40-41 34.25699107243332 37.5 34.5 38.0 23.5 38.0 42-43 34.61910611800893 38.0 35.5 38.0 25.5 38.0 44-45 33.64711729622266 37.0 34.0 38.0 22.0 38.0 46-47 34.39895532465584 37.0 34.5 38.0 25.0 38.0 48-49 34.131297122922845 37.0 34.0 38.0 24.0 38.0 50-51 34.096632469334935 37.0 34.0 38.0 24.0 38.0 52-53 34.12808526201283 37.0 34.0 38.0 24.0 38.0 54-55 33.87110356727559 37.0 34.0 38.0 23.0 38.0 56-57 33.96262988109081 37.0 34.0 38.0 23.5 38.0 58-59 33.81930586293559 37.0 34.0 38.0 22.5 38.0 60-61 33.85546625904948 37.0 34.0 38.0 23.5 38.0 62-63 33.92106324318242 37.0 34.0 38.0 23.5 38.0 64-65 33.94651431036423 37.0 34.0 38.0 23.5 38.0 66-67 33.85343598784651 37.0 34.0 38.0 23.0 38.0 68-69 33.413457931655344 37.0 33.5 38.0 21.5 38.0 70-71 33.23933287070032 37.0 32.5 38.0 21.5 38.0 72-73 33.30392644135189 37.0 33.0 38.0 21.5 38.0 74-75 32.84882685021944 37.0 31.5 38.0 20.0 38.0 76 31.886379834202334 36.0 30.0 38.0 19.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -3.0682328034362563 1101 2 -1.0172254126767442 1101 3 -0.001852583750757475 1101 4 -2.4950560050492605 1101 5 -1.1371909701324867 1101 6 -2.0219715355736554 1101 7 -0.9646242152949611 1101 8 0.0819727934748471 1101 9 -0.9415326039027931 1101 10-11 -0.9102889993963856 1101 12-13 -1.1997173355127657 1101 14-15 -0.03903223723063576 1101 16-17 -1.2435375861787463 1101 18-19 0.416592039859097 1101 20-21 -0.6865427093465257 1101 22-23 -0.039317245231330844 1101 24-25 -1.1099890449411092 1101 26-27 -1.2704115723282214 1101 28-29 0.034339574579860255 1101 30-31 -1.3809834689925182 1101 32-33 0.4479463518872606 1101 34-35 -0.9706221702211764 1101 36-37 0.20201933119319904 1101 38-39 -1.1640727262351902 1101 40-41 -0.026497424541496173 1101 42-43 -0.739737618466819 1101 44-45 -0.16496555395585943 1101 46-47 0.6764150118072223 1101 48-49 -0.2800536290845912 1101 50-51 -0.8856620242063116 1101 52-53 0.06131386349215262 1101 54-55 -1.1388804159973134 1101 56-57 -0.5847238291726455 1101 58-59 0.3964150168911331 1101 60-61 0.6749977547617334 1101 62-63 -1.5202822485741478 1101 64-65 0.07896289549002233 1101 66-67 -1.2134815513732136 1101 68-69 0.47993618393285686 1101 70-71 0.3305899405354751 1101 72-73 -0.9220610842047279 1101 74-75 -1.2851295711313355 1101 76 -1.7527397239809375 1102 1 0.819830377772579 1102 2 -0.20318449643264813 1102 3 0.12789037830554406 1102 4 0.0023794440385742632 1102 5 0.32716760071323137 1102 6 0.7191545354177791 1102 7 1.2716933227361622 1102 8 1.7095883439790143 1102 9 0.8319417797307125 1102 10-11 -0.11538021580950897 1102 12-13 -4.733202210490912 1102 14-15 -0.8972242288375583 1102 16-17 -3.2663562607722056 1102 18-19 -0.9046616758254657 1102 20-21 0.4577861360227118 1102 22-23 -1.2220852116590208 1102 24-25 0.6263867783380235 1102 26-27 -5.486664413734061 1102 28-29 0.10821612642458689 1102 30-31 0.5344446361540705 1102 32-33 -6.410522494647687 1102 34-35 -0.6369682680238213 1102 36-37 0.34011165502604257 1102 38-39 0.5703796838598123 1102 40-41 0.46079639139368567 1102 42-43 0.2700397318858023 1102 44-45 -5.548846768620425 1102 46-47 -0.4614878849695998 1102 48-49 0.23511510670837765 1102 50-51 -6.352803715644207 1102 52-53 -1.5850986794932211 1102 54-55 0.9538076996406133 1102 56-57 -1.1893749919633407 1102 58-59 1.1736487990208815 1102 60-61 0.17292862845760482 1102 62-63 -4.786747831135205 1102 64-65 -0.620751506794754 1102 66-67 0.23766083948765981 1102 68-69 -8.231085556464645 1102 70-71 -0.3581959777486077 1102 72-73 0.39122212381531796 1102 74-75 -6.050036023308291 1102 76 -1.8355339187702349 1103 1 -0.19903033332720454 1103 2 -0.01943274417063634 1103 3 0.05108395546879407 1103 4 0.06543538898569068 1103 5 0.21883320540292317 1103 6 -1.6596144208712502 1103 7 0.5814932462738156 1103 8 0.9468000172299398 1103 9 0.08369404174166561 1103 10-11 0.03165686842650928 1103 12-13 -1.3643606149767038 1103 14-15 -0.5340854496862875 1103 16-17 -0.620478855394353 1103 18-19 -0.4470855320762581 1103 20-21 -0.7611255499719363 1103 22-23 -0.6008860272573457 1103 24-25 0.4795918248526121 1103 26-27 -0.3127253287334284 1103 28-29 0.1937010251119773 1103 30-31 0.017170518656932643 1103 32-33 -5.1289817784504095 1103 34-35 -1.2194265323286544 1103 36-37 -0.26891090136494 1103 38-39 0.4768088924745797 1103 40-41 0.5772931417441427 1103 42-43 0.09107961974103773 1103 44-45 -4.6944820432476675 1103 46-47 -0.4766529161917248 1103 48-49 -0.20683723176492208 1103 50-51 -0.33147072214350715 1103 52-53 -0.2365642077205692 1103 54-55 -0.24068701171404427 1103 56-57 0.6555402622739521 1103 58-59 0.6428281032135601 1103 60-61 0.5074704915900128 1103 62-63 0.35215238550809147 1103 64-65 0.6160013924023886 1103 66-67 -0.00939536033392585 1103 68-69 -2.8437400741147023 1103 70-71 0.3576954457253336 1103 72-73 0.8039276163602409 1103 74-75 -1.6347945297438713 1103 76 0.3295227908003042 1104 1 -2.0311114096857565 1104 2 0.3009237122333488 1104 3 0.030313963040580205 1104 4 0.17053178365049604 1104 5 -0.07441625583535583 1104 6 -0.26999359263430733 1104 7 1.111576535923767 1104 8 0.9811176827899644 1104 9 -0.49455745805447293 1104 10-11 -0.67523345716031 1104 12-13 -0.6142062670239952 1104 14-15 -0.1520946523189437 1104 16-17 -0.27158841331852557 1104 18-19 1.243711835271867 1104 20-21 1.4596852136135965 1104 22-23 0.4929691768896731 1104 24-25 0.40028027496619245 1104 26-27 0.7159426193124787 1104 28-29 -0.6849523618063031 1104 30-31 -1.3579474264767342 1104 32-33 -0.6595019019287776 1104 34-35 -0.03513507679833339 1104 36-37 0.13143274124674775 1104 38-39 0.060593620763938816 1104 40-41 0.8164858728292401 1104 42-43 0.7741479405517993 1104 44-45 -2.7471001339926175 1104 46-47 0.4399409448368772 1104 48-49 0.8173051178186341 1104 50-51 1.5795141925282863 1104 52-53 0.523852822974284 1104 54-55 -0.5842772033384804 1104 56-57 0.40770404583734887 1104 58-59 0.9370327744233009 1104 60-61 0.07484191683317931 1104 62-63 0.45758077388249774 1104 64-65 0.13046932879938566 1104 66-67 0.14374693100511138 1104 68-69 -3.5713304987171846 1104 70-71 -0.05393015856146377 1104 72-73 1.2033684690598747 1104 74-75 -0.5391453946748541 1104 76 0.5867072511649098 1105 1 -1.1321287279124235 1105 2 0.10780336927121681 1105 3 -1.0377340788770155 1105 4 -0.13973326347277393 1105 5 0.08573481081769785 1105 6 0.38408418627760454 1105 7 0.9494520760551026 1105 8 0.6762624549991401 1105 9 0.8082563284788193 1105 10-11 1.179875249586658 1105 12-13 -1.0511257321663905 1105 14-15 0.46651308474342557 1105 16-17 0.653269536393978 1105 18-19 0.7210858613342666 1105 20-21 0.0903204420849022 1105 22-23 0.23203896015665748 1105 24-25 0.4537149998819814 1105 26-27 0.5645527380562996 1105 28-29 -0.6416908191571906 1105 30-31 -0.12534220719192035 1105 32-33 0.6062086897489465 1105 34-35 0.7401192527583262 1105 36-37 0.9192079105162421 1105 38-39 0.441288391271911 1105 40-41 0.5650483579437307 1105 42-43 1.0150209969296 1105 44-45 1.8785335633977382 1105 46-47 1.2281073903474038 1105 48-49 0.5768233376818515 1105 50-51 0.06558135594566039 1105 52-53 -1.0090484052928659 1105 54-55 -0.650028623841358 1105 56-57 -0.0053815926234079825 1105 58-59 0.31204553343116004 1105 60-61 0.39951613683250287 1105 62-63 0.6826496725249314 1105 64-65 0.39844251787086904 1105 66-67 0.5132818226386036 1105 68-69 1.0587603454187757 1105 70-71 1.4074185536293413 1105 72-73 0.8749686187655712 1105 74-75 -1.0280875878632436 1105 76 -0.8740460406591168 1106 1 -0.7680732047130441 1106 2 0.3584585818512984 1106 3 -0.5849579189080778 1106 4 0.5005550300624577 1106 5 0.6005256281347258 1106 6 0.16826444253852202 1106 7 0.8819045124752165 1106 8 1.4796016124442986 1106 9 1.8867847695582185 1106 10-11 0.9487368783965948 1106 12-13 0.42036645381691784 1106 14-15 0.4757163947803065 1106 16-17 1.0179620718239875 1106 18-19 0.3782883462886417 1106 20-21 0.17305316388649317 1106 22-23 0.9001919886948002 1106 24-25 0.8469335496781341 1106 26-27 0.43991674504524525 1106 28-29 -0.6857356648182318 1106 30-31 0.23714808595504167 1106 32-33 0.9222395575416087 1106 34-35 1.062708313134955 1106 36-37 1.135500863340944 1106 38-39 0.6130875564825544 1106 40-41 0.5712425435387587 1106 42-43 1.1883004572285714 1106 44-45 1.3595869996237155 1106 46-47 0.9109966351542411 1106 48-49 0.8584980125022739 1106 50-51 0.9348179119541982 1106 52-53 0.04052327981681714 1106 54-55 0.7610141348462705 1106 56-57 1.2021691256837173 1106 58-59 0.09054121388155778 1106 60-61 1.3617140110316868 1106 62-63 0.8145900724472739 1106 64-65 1.1167678430504466 1106 66-67 1.0186205918096434 1106 68-69 1.5939780215981543 1106 70-71 1.3420610987662087 1106 72-73 1.0111497001089944 1106 74-75 -0.8598132236621154 1106 76 -0.10858787719570984 1107 1 0.09388436520016441 1107 2 -0.31634855411784457 1107 3 0.253084511178713 1107 4 -0.06721256878035575 1107 5 -0.3472684895123308 1107 6 0.6716998330881836 1107 7 0.5351470389940189 1107 8 1.0321570608531658 1107 9 -0.7206404956298229 1107 10-11 -0.47239981398527675 1107 12-13 -0.0471058219786471 1107 14-15 -0.7894897286526685 1107 16-17 0.15798738522804 1107 18-19 0.5683076326917273 1107 20-21 0.4687438581970014 1107 22-23 -0.7132783584604567 1107 24-25 -0.09926341271337691 1107 26-27 0.16151751650136248 1107 28-29 -1.0514781913370328 1107 30-31 -0.31267110401602594 1107 32-33 0.7143080242725617 1107 34-35 0.2844356516113251 1107 36-37 -0.8984190980862508 1107 38-39 0.4089289258171718 1107 40-41 0.3288908077624839 1107 42-43 0.2831051823973283 1107 44-45 0.17528733713577083 1107 46-47 0.27311090709252284 1107 48-49 0.16918388521201422 1107 50-51 0.9029832928606609 1107 52-53 0.9103099239826875 1107 54-55 0.06656500273441424 1107 56-57 0.6048873031186233 1107 58-59 0.6328808956559087 1107 60-61 -0.30158758009656594 1107 62-63 -0.7575169670898418 1107 64-65 0.25034426542267596 1107 66-67 1.3736832726196653 1107 68-69 0.9146928239125245 1107 70-71 0.0034581575897405514 1107 72-73 0.09542525459308138 1107 74-75 0.10591122185379476 1107 76 -0.8019581761349599 1108 1 -1.5664925724601453 1108 2 -0.5447527026625067 1108 3 -0.4361984151065563 1108 4 -0.36264024804878403 1108 5 -0.4234589657028067 1108 6 -0.23072015129901047 1108 7 0.9550087541718923 1108 8 0.25971365726222473 1108 9 -0.3008903004697885 1108 10-11 -0.1611195641804386 1108 12-13 0.45986975513744 1108 14-15 0.3308459466010376 1108 16-17 -1.0881428701528009 1108 18-19 -0.07249196583620687 1108 20-21 -0.28344193415271945 1108 22-23 -0.6108249180679053 1108 24-25 -1.0115473426788029 1108 26-27 -0.7214478007730847 1108 28-29 0.217775739743594 1108 30-31 0.32400782985259724 1108 32-33 0.1559830549405703 1108 34-35 -0.4472081534516761 1108 36-37 0.1987761734649922 1108 38-39 0.526878072689037 1108 40-41 0.331634200201421 1108 42-43 0.5866347909620657 1108 44-45 -0.021277846314660565 1108 46-47 0.27311648309341763 1108 48-49 -0.11200268777783862 1108 50-51 0.1860434021502826 1108 52-53 -0.1997603336285536 1108 54-55 -0.02086111525376566 1108 56-57 0.7306930352475405 1108 58-59 -1.542874652464981 1108 60-61 0.9811993051493317 1108 62-63 -0.10092613403531203 1108 64-65 0.27604405353464045 1108 66-67 -0.5322997763376165 1108 68-69 0.8282592340831556 1108 70-71 1.609580606569331 1108 72-73 -0.07852366923874143 1108 74-75 1.2689757919441291 1108 76 1.081938333288484 1109 1 -0.3690793966635084 1109 2 -0.7562860001920448 1109 3 -0.4675043585365586 1109 4 -0.3369062702471375 1109 5 0.0395736157508253 1109 6 0.3211282196283065 1109 7 -1.6662766020758681 1109 8 0.31861090036999684 1109 9 1.0199117045427428 1109 10-11 0.9858885811829694 1109 12-13 0.10138425567450327 1109 14-15 0.2797024628917626 1109 16-17 -0.4512716743060423 1109 18-19 0.2562875722948377 1109 20-21 1.0992335044437667 1109 22-23 1.1303583973635298 1109 24-25 1.0044412896162171 1109 26-27 0.46845776671706574 1109 28-29 0.31310334446969534 1109 30-31 0.5931852376578277 1109 32-33 0.8002387835478046 1109 34-35 -0.2960378168960034 1109 36-37 0.028786467056114873 1109 38-39 0.6051541199500434 1109 40-41 0.32569300793503686 1109 42-43 0.4060829649685047 1109 44-45 -0.0622396257632758 1109 46-47 -0.4828694639169697 1109 48-49 -0.0010221113367379075 1109 50-51 1.1591793670625563 1109 52-53 1.0828994408075303 1109 54-55 0.2933283763323473 1109 56-57 0.4158881659313991 1109 58-59 0.357859325336662 1109 60-61 0.7953440419914415 1109 62-63 -0.2996707634660325 1109 64-65 -0.33127782258422656 1109 66-67 0.19818715527713238 1109 68-69 1.1904369999263267 1109 70-71 -0.22285763188394725 1109 72-73 -0.4576970491170087 1109 74-75 1.5037703676691478 1109 76 -0.1668309112514379 1110 1 0.3691145471000077 1110 2 -0.027419742081015386 1110 3 0.06210090462133522 1110 4 0.7655630880801851 1110 5 0.37122466117259734 1110 6 0.33793607831611894 1110 7 0.7782078968426589 1110 8 1.8234775189712877 1110 9 -1.358783320691579 1110 10-11 -1.1859075615711703 1110 12-13 0.14923933858726457 1110 14-15 -0.1184365061060717 1110 16-17 0.23917983975307777 1110 18-19 0.513159418005003 1110 20-21 -0.6949716145180247 1110 22-23 0.11566841899295355 1110 24-25 0.09539749004880349 1110 26-27 -1.7630445764696674 1110 28-29 -0.05378562452814606 1110 30-31 -0.15361541122465638 1110 32-33 1.0211517251483784 1110 34-35 0.26720569427919116 1110 36-37 -0.18329446300877095 1110 38-39 0.535579498464827 1110 40-41 0.43972058544044756 1110 42-43 0.39957160952511117 1110 44-45 1.2704703598171037 1110 46-47 -0.09550014163866649 1110 48-49 0.541943822333053 1110 50-51 0.10841783137401961 1110 52-53 -0.154505930497443 1110 54-55 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46-47 0.5675073303414067 2116 48-49 0.48817047239532485 2116 50-51 0.5357403718472469 2116 52-53 1.3012786274104045 2116 54-55 0.4195903380548316 2116 56-57 0.4951704625821165 2116 58-59 -0.3005617272949124 2116 60-61 -1.334255240305211 2116 62-63 0.884509858543538 2116 64-65 0.7270953831573976 2116 66-67 0.10268476293263262 2116 68-69 0.45028149753398594 2116 70-71 -2.8347236605920827 2116 72-73 0.5536964915528486 2116 74-75 -1.3305418332877856 2116 76 -1.5897376098160336 2117 1 0.49673847535466464 2117 2 0.2303904481229857 2117 3 0.6502304010242135 2117 4 0.1642769936680466 2117 5 -0.45086561181448914 2117 6 -0.8804289556840743 2117 7 -2.2860229102924556 2117 8 -1.4490907278011527 2117 9 0.34933237852986565 2117 10-11 0.13958273112617547 2117 12-13 1.0619105714639758 2117 14-15 -0.2645634401752517 2117 16-17 0.6874896850275434 2117 18-19 0.7911110293186923 2117 20-21 0.20456765817341704 2117 22-23 0.38949319478874855 2117 24-25 0.8331401726150816 2117 26-27 1.026141882582273 2117 28-29 0.6031449953595143 2117 30-31 0.22786433648403204 2117 32-33 1.4888392189889217 2117 34-35 0.22678734402789047 2117 36-37 -0.8408533474081068 2117 38-39 0.617980700790639 2117 40-41 -0.8518557425382625 2117 42-43 -0.2819968343499255 2117 44-45 0.6604624757134303 2117 46-47 0.14689908966122545 2117 48-49 -0.6961068330330633 2117 50-51 0.8377220592246175 2117 52-53 1.3963342193005275 2117 54-55 -0.6141048875828332 2117 56-57 -1.0005260711000332 2117 58-59 -0.42602935319419544 2117 60-61 1.1700482260126392 2117 62-63 0.001481735587354649 2117 64-65 -0.44373522508328733 2117 66-67 -1.5344898610045803 2117 68-69 0.19817554593163322 2117 70-71 1.00669556766168 2117 72-73 1.5143897551238084 2117 74-75 0.737666145783507 2117 76 -0.7043877079193663 2118 1 0.12564811360151396 2118 2 0.36435136449627237 2118 3 0.6028810464653098 2118 4 0.41967267712128375 2118 5 -0.43308409234921186 2118 6 0.8270306085794061 2118 7 1.1083594561548153 2118 8 -3.287398804743855 2118 9 -1.3875164402874205 2118 10-11 -0.4587386117994825 2118 12-13 0.6771587984143324 2118 14-15 -0.536681915607673 2118 16-17 0.05181949777309569 2118 18-19 -0.7495798222553702 2118 20-21 -2.7479809412449256 2118 22-23 -0.4111216324983573 2118 24-25 0.27167019965727945 2118 26-27 0.5981353516368131 2118 28-29 -0.00837855176023794 2118 30-31 -0.44299897286848733 2118 32-33 -0.016798823768361615 2118 34-35 0.9593629856422581 2118 36-37 -0.1430243765422503 2118 38-39 -0.07863647355499381 2118 40-41 -1.2929496759022143 2118 42-43 -0.4957577405186129 2118 44-45 0.3905762570288047 2118 46-47 0.07873234646004335 2118 48-49 -1.0084059299257575 2118 50-51 -0.41502042763695357 2118 52-53 -1.1792436163033315 2118 54-55 0.5666696417298809 2118 56-57 1.046440610873539 2118 58-59 0.6791545847618181 2118 60-61 -1.761708496269108 2118 62-63 0.573194578079324 2118 64-65 -0.041150909556975535 2118 66-67 -2.17266017581656 2118 68-69 1.2320803369924036 2118 70-71 -0.49973333177196366 2118 72-73 -1.6295078927153668 2118 74-75 0.635454298240262 2118 76 0.7931121645231016 2119 1 -0.11903273746231591 2119 2 0.14803930775868324 2119 3 -0.1417998045985236 2119 4 -0.06968902500637597 2119 5 0.48180952467206595 2119 6 0.5468887646077789 2119 7 -1.6966050828522796 2119 8 0.7019628973838081 2119 9 -0.0896440998618857 2119 10-11 0.3249493314629248 2119 12-13 0.34559851472638314 2119 14-15 -0.5863273056785943 2119 16-17 -0.43045000577300385 2119 18-19 -0.8222748577163657 2119 20-21 -1.5689029270605275 2119 22-23 0.40270815473568433 2119 24-25 -0.22301065140654686 2119 26-27 0.023667266530431164 2119 28-29 0.394103717743306 2119 30-31 -0.34016209343585757 2119 32-33 -0.1426853485910513 2119 34-35 0.4487246877699178 2119 36-37 -0.6075633836344494 2119 38-39 -0.4899839912854915 2119 40-41 -0.7663539312213601 2119 42-43 -0.749303839809393 2119 44-45 -1.2955939557371536 2119 46-47 -0.3645300648874752 2119 48-49 -0.779682525670438 2119 50-51 0.4715043950580835 2119 52-53 -1.9061939709132574 2119 54-55 -1.8978693653623182 2119 56-57 0.39573139101538146 2119 58-59 1.11709784717317 2119 60-61 0.5627595888372738 2119 62-63 -0.712266414828477 2119 64-65 0.18048029611677663 2119 66-67 0.3982618099990418 2119 68-69 -0.28387710981611036 2119 70-71 -0.7515063814173502 2119 72-73 -2.0444015097366446 2119 74-75 -0.8893683967951986 2119 76 0.420771738991192 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 14.0 13 99.0 14 272.0 15 468.0 16 665.0 17 751.0 18 775.0 19 814.0 20 760.0 21 737.0 22 708.0 23 827.0 24 921.0 25 1024.0 26 1230.0 27 1613.0 28 1927.0 29 2372.0 30 2990.0 31 3776.0 32 4777.0 33 6399.0 34 8700.0 35 13092.0 36 22414.0 37 28509.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.21849374598496 21.861655896912673 10.946415750292863 30.97343460680951 2 18.014554184327995 21.092314040286585 34.95911324505796 25.934018530327467 3 17.160246070745337 23.925316028358154 28.939570126411347 29.974867774485165 4 13.213173787463898 17.51566075246633 37.38887430136164 31.882291158708124 5 12.727409130124911 36.15289395701264 35.987846505870436 15.131850406992008 6 28.61416407217075 36.935931580329346 18.085824674593944 16.36407967290596 7 27.44570313965265 29.416894857271465 22.805619115495706 20.33178288758018 8 23.28763269439964 33.45024194455906 20.769721294872276 22.492404066169023 9 23.124460782474962 16.170899133500882 22.217637570801603 38.48700251322255 10-11 24.276041862035335 25.46841591957688 27.253929254660715 23.001612963727073 12-13 23.49675531715368 24.148974080048013 26.31381522187629 26.04045538092202 14-15 21.28830788851795 25.561255110844368 24.231497805619114 28.918939195018567 16-17 21.8800405116471 28.0224314490416 26.22519599384823 23.87233204546307 18-19 21.180933268314643 27.47993173037248 26.929460970028885 24.409674031283995 20-21 21.076840841742 27.731253985520837 26.82067969541243 24.37122547732473 22-23 22.111669605011443 27.3519261787764 27.248302637008138 23.28810157920402 24-25 21.275179113995275 28.166379083986648 26.77519786938745 23.783243932630633 26-27 20.402115608237366 28.517104917663826 27.60371731872914 23.47706215536967 28-29 21.451948685247007 27.205634119809446 28.47349863085637 22.868918564087178 30-31 21.54947672455831 27.15405679132751 27.701714242844815 23.594752241269365 32-33 20.24269477474774 28.399883716568514 27.451798642109605 23.905622866574138 34-35 21.65591357515286 27.25252260024757 27.756104880153043 23.335458944446525 36-37 21.960688698000673 27.61731497805619 27.048557710341726 23.37343861360141 38-39 21.140609175137854 27.749540492891704 27.928185603360966 23.181664728609476 40-41 21.43319329307176 26.57029520987284 28.04118684121685 23.955324655838552 42-43 20.9718106455606 25.861341385648373 28.53914250346975 24.62770546532128 44-45 20.472448328894558 25.855714767995796 29.074140065268768 24.59769683784088 46-47 20.1761131325256 25.7005138977456 29.617577553546642 24.505795416182153 48-49 19.511703364717356 25.6981694737237 30.405772909711544 24.384354251847405 50-51 18.754923290446005 26.28708878802656 29.851082186128515 25.106905735398925 52-53 18.519543118646613 26.40712329794816 30.140852995236127 24.9324805881691 54-55 18.05722270152669 26.966971754379383 29.337653325331033 25.638152218762894 56-57 18.239150005626616 26.81974192580367 29.261225102216887 25.679882966352825 58-59 18.01314752991485 26.624216962376686 29.25184740612926 26.110788101579203 60-61 18.859015717018643 25.970591545069205 29.0431936681796 26.127199069732548 62-63 18.886211035672755 25.956056116133386 28.95363667054278 26.204096177651078 64-65 19.831482801305373 25.80882628755767 27.56104880153044 26.79864210960651 66-67 19.848362654263102 25.90494767245583 26.884448028808283 27.362241644472785 68-69 19.667373119771934 25.377921152331297 26.889605761656476 28.065099966240293 70-71 20.72236392962977 25.115345661877793 26.325068457181438 27.837221951311005 72-73 21.096534003526013 24.756648786526124 26.171743126148765 27.97507408379909 74-75 21.153269064856143 25.030946397089167 26.2209760306088 27.59480850744589 76 21.869725045950712 24.13256311189467 26.293184290483513 27.704527551671106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.5 11 0.5 12 0.0 13 1.0 14 2.0 15 4.0 16 7.5 17 9.0 18 11.5 19 17.0 20 26.0 21 32.0 22 46.0 23 97.0 24 146.0 25 158.0 26 210.5 27 330.5 28 461.5 29 525.0 30 608.0 31 867.5 32 1321.5 33 1599.0 34 1739.0 35 2268.0 36 2947.5 37 3238.0 38 3591.5 39 4493.5 40 5551.0 41 6324.0 42 6588.0 43 6855.5 44 7375.0 45 7803.5 46 7980.0 47 7921.0 48 7648.0 49 7161.0 50 6888.0 51 6439.5 52 5401.0 53 4440.5 54 4070.0 55 3793.5 56 3071.0 57 2336.5 58 2048.0 59 1697.0 60 1187.0 61 857.0 62 686.0 63 553.5 64 343.5 65 228.0 66 153.0 67 116.0 68 88.0 69 46.5 70 34.0 71 35.0 72 25.5 73 17.5 74 14.5 75 10.0 76 9.5 77 9.5 78 8.5 79 7.0 80 6.0 81 4.0 82 4.0 83 5.0 84 4.0 85 3.0 86 1.5 87 0.0 88 1.0 89 2.5 90 2.0 91 1.0 92 1.0 93 0.5 94 0.5 95 2.0 96 3.0 97 2.0 98 0.5 99 4.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7352113732698151 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 106636.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.987133800967776 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29106914154806 37.58205484076672 2 10.855565777368906 11.286995011065681 3 5.792160470443927 9.033534641209348 4 3.4399408337391995 7.153306575640498 5 2.000469000847809 5.19993248058817 6 1.33484856684164 4.163697062905585 7 0.9271785991305446 3.374095052327544 8 0.6728358316647727 2.7983045125473573 9 0.5393509749806087 2.523538017179939 >10 2.1411692551905768 16.704490040886753 >50 0.005411548243952595 0.1800517648824037 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.007502156870100154 0.0 2 0.0 0.0 0.0 0.007502156870100154 0.0 3 0.0 0.0 0.0 0.009377696087625191 0.0 4 0.0 0.0 0.0 0.014066544131437788 0.0 5 0.0 0.0 0.0 0.017817622566487865 0.0 6 0.0 0.0 0.0 0.017817622566487865 0.0 7 0.0 0.0 0.0 0.018755392175250383 0.0 8 0.0 0.0 0.0 0.026257549045350537 0.0 9 0.0 0.0 0.0 0.03844855395926329 0.0 10 0.0 0.0 0.0 0.06095502456956375 0.0 11 0.0 0.0 0.0 0.06939495104842643 0.0 12 0.0 0.0 0.0 0.06939495104842643 0.0 13 0.0 0.0 0.0 0.06939495104842643 0.0 14 0.0 0.0 0.0 0.0731460294834765 0.0 15 0.0 0.0 0.0 0.07877264713605162 0.0 16 0.0 0.0 0.0 0.08627480400615177 0.0 17 0.0 0.0 0.0 0.0881503432236768 0.0 18 0.0 0.0 0.0 0.10034134813758956 0.0 19 0.0 0.0 0.0 0.10503019618140215 0.0 20 0.0 0.0 0.0 0.10596796579016467 0.0 21 0.0 0.0 0.0 0.10878127461645223 0.0 22 0.0 0.0 0.0 0.12378558835665254 0.0 23 0.0 0.0 0.0 0.1322255148355152 0.0 24 0.0 0.0 0.0 0.1359765932705653 0.0 25 0.0 0.0 0.0 0.1416032109231404 0.0 26 0.0 0.0 0.0 0.1509809070107656 0.0 27 0.0 0.0 0.0 0.1809895344911662 0.0 28 0.0 0.0 0.0 0.21568701001537943 0.0 29 0.0 0.0 0.0 0.25038448553959264 0.0 30 0.0 0.0 0.0 0.3019618140215312 0.0 31 0.0 0.0 0.0 0.35916576015604484 0.0 32 0.0 0.0 0.0 0.41261862785550846 0.0 33 0.0 0.0 0.0 0.44731610337972166 0.0 34 0.0 0.0 0.0 0.5242132112982483 0.0 35 0.0 0.0 0.0 0.632056716305938 0.0 36 0.0 0.0 0.0 0.8242994861022545 0.0 37 0.0 0.0 0.0 1.2256648786526125 0.0 38 0.0 0.0 0.0 1.7695712517348738 0.0 39 0.0 0.0 0.0 2.422258899433587 0.0 40 0.0 0.0 0.0 3.2024832139240034 0.0 41 0.0 0.0 0.0 4.284669342435951 0.0 42 0.0 0.0 0.0 5.4962676769571255 0.0 43 0.0 0.0 0.0 6.83821598709629 0.0 44 0.0 0.0 0.0 8.080760718706628 0.0 45 0.0 0.0 0.0 9.206084249221652 0.0 46 0.0 0.0 0.0 10.392362804306238 0.0 47 0.0 0.0 0.0 11.46517123673056 0.0 48 0.0 0.0 0.0 12.386998762144117 0.0 49 0.0 0.0 0.0 13.264751115945835 0.0 50 0.0 0.0 0.0 14.070295209872839 0.0 51 0.0 0.0 0.0 15.06057991672606 0.0 52 0.0 0.0 0.0 16.047113545144228 0.0 53 0.0 0.0 0.0 17.228703252185003 0.0 54 0.0 0.0 0.0 18.33620916013354 0.0 55 0.0 0.0 0.0 19.356502494467158 0.0 56 0.0 0.0 0.0 20.24175700513898 0.0 57 0.0 0.0 0.0 21.096065118721633 0.0 58 0.0 0.0 0.0 21.83971641847031 0.0 59 0.0 0.0 0.0 22.601185340785477 0.0 60 0.0 0.0 0.0 23.28763269439964 0.0 61 0.0 0.0 0.0 24.05472823436738 0.0 62 0.0 0.0 0.0 24.781499681158333 0.0 63 0.0 0.0 0.0 25.628305637870888 0.0 64 0.0 0.0 0.0 26.48542706027983 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTAAGT 15 0.0022202407 69.992966 16 AAGTCAA 25 2.3823585E-4 55.994373 64 CTAGATA 20 0.006937592 52.494724 43 TCCATGT 20 0.006937592 52.494724 30 ACGCCTT 20 0.006937592 52.494724 10 GATGTAT 20 0.006937592 52.494724 24 CGTGCGA 20 0.006937592 52.494724 10 CGCGAGA 20 0.006937592 52.494724 32 AGCGGTC 20 0.006937592 52.494724 7 GAGCGGT 20 0.006937592 52.494724 6 TCTAATT 20 0.006937592 52.494724 37 GGTATTC 20 0.006937592 52.494724 3 TGGATGT 20 0.006937592 52.494724 36 CGATGGC 20 0.006937592 52.494724 14 CAGAGCG 20 0.006937592 52.494724 4 GGCTCGC 20 0.006937592 52.494724 28 AGAGCGG 20 0.006937592 52.494724 5 CAAGTCA 30 5.8582E-4 46.661976 63 CCTCGGA 45 1.6724916E-6 46.661976 45 CTGCGCC 35 0.0012512697 39.99598 13 >>END_MODULE