FastQCFastQC Report
Wed 25 May 2016
SRR1780620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106636
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT11721.0990659814696724TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT4690.4398139465096216TruSeq Adapter, Index 5 (96% over 26bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1560.146292058966953No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC1560.146292058966953RNA PCR Primer, Index 5 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA207.8957164E-570.0000138
GTATTTG150.002219356670.047
GACACGT150.002219356670.037
ATCGTAC150.002219356670.025
TAACCGC150.002219356670.030
TCGTACT150.002219356670.026
GACATCG150.002219356670.022
CACGTAC150.002219356670.039
GAGATGA150.002219356670.011
GCCATAG252.3811753E-456.0000041
CGAGCAA252.3811753E-456.00000437
TATTTGC200.006934833252.50000448
GTCCTAT200.006934833252.50000429
GACAAAG200.006934833252.50000447
CGACACG200.006934833252.50000436
CATCGTA200.006934833252.50000424
ACGTACT200.006934833252.50000440
ACATCGT200.006934833252.50000423
TGTAAGC200.006934833252.5000042
ACGACAC200.006934833252.50000435
Produced by FastQC (version 0.11.5)