FastQCFastQC Report
Wed 25 May 2016
SRR1780619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGGTCCCCATCACCTTCCCGCAAGCTGTCTCTTATACACATCTCC10.684931506849315No Hit
CCCTTCCCGCACTAATTAATCCCCTGGCCCAACCCGTCATCTACTCTACC10.684931506849315No Hit
ACGGCGGCGTCCGCCTCGTGGATCAGGTGGCCGAGCTCCTGGGGCGTGTG10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGAAACAAAATCTCGTATGCCGTCTT10.684931506849315TruSeq Adapter, Index 6 (95% over 21bp)
TTTTTTATTGTGTCTATTTTATTCTTCTCTCTTTTCTTCTTTATTAGTCT10.684931506849315No Hit
CTGAAATCTAGGTGGAAGTTCCCAAACCCCAGTTCTTTACTTCTGTGCAC10.684931506849315No Hit
GTCCCGGCCCGTCCCGCAGGAGGCGGACGCCGACGCCGTCGGCACGGTCG10.684931506849315No Hit
CGCCGCCCCCTACGCCCACGCCACCGCTCCGCTGCGCCGGCTCGTGGACC10.684931506849315No Hit
ACCCAGAACCACGCCTGGCCCACCGAGATGCCGACCACGTAGGCCGCCAG10.684931506849315No Hit
GCCGCGCAGAGCTCGCGCGCCGCAGCGTCGTCCTCCTGTCTCTTATACAC10.684931506849315No Hit
GAGCCAGTGCGCCAGGGGCAGGCCGGACGGATGCTGGACCTGCCTCTTAG10.684931506849315No Hit
GACCTGCTCCGCCGGGATGCCGGCGGCCTCGGCCTCGGCCAGTGCGGCGT10.684931506849315No Hit
GCACCAGCTACACCACGTCCATGAACTTCAACGTGGGCACCGCCGTGCCT10.684931506849315No Hit
GCTCACCACGCTGCCCCCGGCCGTGTTCGGCTACGTCGCGCCGGTGCTGC10.684931506849315No Hit
TGTTTGTGCCTGTCTCTTATACACATCTCCGAGCCCAGAGACGATCCAAA10.684931506849315No Hit
GCGTCACCCTGGACGAGCAGACGCCCGCGGCGGACGTCCCGGCCTGTCTC10.684931506849315No Hit
GGATCAGGTCTCCGCGGCCGATGCCGAGGGTGCGGCGGCGGCCGAGGTCG10.684931506849315No Hit
CTCTCCCTAAGCTTCAAACTAGACTACTTCTCCATAATATTCATCCCTGT10.684931506849315No Hit
GAACTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAAT10.684931506849315No Hit
GTTTGGGTTGTGGCTCAGTGTCAGTTCGAGATAATAACTTCTTGGTCTAG10.684931506849315No Hit
GTTTAGTAATGGGGTTTGTGGGGTTTTCTTCTAAGCCCTGTCTCTTATAC10.684931506849315No Hit
GCCGAGGCCCGCGCCGGACGGGGCGCCGAGATCACCGGCGCCTCCGCGCA10.684931506849315No Hit
GTACCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCT10.684931506849315No Hit
GGACGAGCACGGTGTCGCCGGACTTGAGCGCGTTCTCGATCTTCCTCGGC10.684931506849315No Hit
CCTTTGCGTAGTTGTATATAGCCTAGAATTTTTCGTTCGGTAAGCATTAG10.684931506849315No Hit
GCTCAAGACCGTGCTGCAGACCGTGGGCATCGGCCTGCTGCCTGTCTCTT10.684931506849315No Hit
GCCTCCCACAGAAGGTGGCAGAAAGACTTGATGAAATATTTCAGACAGGT10.684931506849315No Hit
GCTGCTGCTCCTGCGGGGCCAGCGGCACGGTGCCGCGCCTGTCTCTTATA10.684931506849315No Hit
ATCCAAGCCTCACCCCACTACTAGGCCTCCTCCTAGCAGCAGCAGGCAAA10.684931506849315No Hit
CCTTTGAGTTGAACACACCATGGTAGTATCTCATGGCATAGTTATCCTGA10.684931506849315No Hit
CTGTCTCTTATACACATCTCCGAGACCACGAGACGATCCAAAATCTCGTA10.684931506849315No Hit
CGCACGCTCGGCGTGCCCGAGGATGCGGCGACTCCGCTCGACGGCCTGTG10.684931506849315No Hit
GCCCTGATCGGCTCCACGGGCTCGATCGGCACCCAGGGCCTCGACGTGAT10.684931506849315No Hit
ATCTCCGACCCACGAGACCCAACACGATATCGTATGCCGTCTTCTGCTTG10.684931506849315Illumina PCR Primer Index 1 (96% over 28bp)
GGCTAAGAGGGAGTGGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACA10.684931506849315No Hit
CCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCTCGCT10.684931506849315No Hit
GTCCACGGTGTCGATGCGCCGCTGCCGGCCCCCCACGGCGATCCGCAGGC10.684931506849315No Hit
GTTCTAGGGGGAGTGGCAGGACGGTTGTTCATAGGAGGCGCATCAGTTGG10.684931506849315No Hit
GGTCTCCCTGCCGCTCACCGCCTGGCGGAACGTCGCCACGACCCTGGACG10.684931506849315No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT10.684931506849315RNA PCR Primer, Index 19 (95% over 23bp)
CAAATATTTAAAATAAGTTTAATTACCAAGAACTACTGGTCTTCGAGCCC10.684931506849315No Hit
GCCTCGAACCGCTCCCCCAGGCCGAGGGCGATCGCCTGCGTGCGGAGGTT10.684931506849315No Hit
CTTATACACATCTCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTT10.684931506849315No Hit
CCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTATACAC10.684931506849315No Hit
AGTTTACCCATCCATGAATCCTGAGGGTGCATTAAGCTACATCATAACAG10.684931506849315No Hit
CTGCCGGGCGGAGCGGTGCCACCGTTCCGTGCCCGCCGCGTGGGGCGGCA10.684931506849315No Hit
GCAACGTGCTCCGAGATGCGGTGGAAGTCCGCACCGGCGGTGGCAACCCC10.684931506849315No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTG10.684931506849315No Hit
CCTCAGAATGATATTTGGCCTCACGGGAGGACATAGCCTATGAAGGCTGT10.684931506849315No Hit
GGCTTAGAGCTGTGCCTAGGACTCCAGCTCATGCGCCGAATAATAGGTAT10.684931506849315No Hit
GTGATGTACATCGCGCGGCACCAGCTCTTCCCATTGGCTTTCACCGTAGA10.684931506849315No Hit
GTGTTTGTAGTATTCTCTGATGGTAGTTTGTATTTCTGTGGGATTGGTGG10.684931506849315No Hit
GCGTCGCACCCTCACCCGCGGCTCCGTGCCCCTTGCCACGCTGGCCGGCG10.684931506849315No Hit
CCCTCACCCTGGCCCCTGCACCTGCCCATCTGCATGCTCCCCTCCCATAA10.684931506849315No Hit
ACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGAGGCTTA10.684931506849315No Hit
TCCGAGTGCTGCTCCTCGATGCGCCAGTCGACGAAGGAGCAGCAGGAGCT10.684931506849315No Hit
CCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGCGCTAAG10.684931506849315No Hit
CACCGCGGACGTGCGCTCCGAGCCCACGAGACTAACAACAATCTCGTATG10.684931506849315No Hit
GGGCGTGGCTGCTGGCCCTCGGCGTGCACTACGTGGCCGGGCTCGCCGTG10.684931506849315No Hit
AGGCCCACCGCTGCTTCGCAGGCGGCAAAGACTAGTATGGCAATAGGCAC10.684931506849315No Hit
TGGTAGATGTGGCGGGGTTTTTGGGGCCTTTTGTGAAGAGGTTTATTGCG10.684931506849315No Hit
ATAATGAGAGCGTCTCAGAGCTACGAAAGTGGGAAATGAACTCCTGCAGC10.684931506849315No Hit
CTTATACACATCTCCGAGCCCAGACATGATGATATCTCGTATGCCGTCTT10.684931506849315TruSeq Adapter, Index 25 (95% over 22bp)
CAATATGAAATGATCTGCTGCAGTGCTCCGAGCCCACGAGACTTTAATGC10.684931506849315No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA10.684931506849315No Hit
TCGGCAGCGTCAGATGTGTATAAATCTCCGAGCCCACGAGACTAACAACA10.684931506849315No Hit
GGCCGACGGCGGCTTCGCCGAGCCCACGAGACTAACAACAATCTCGTATG10.684931506849315No Hit
ATTCTGGTCAGTATCAAGGGAAGCTAGCTCAGCCAGGCTCCTCTGCTGGG10.684931506849315No Hit
CTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCT10.684931506849315No Hit
CATTACCATCGAGTTCAGAGAATGGCAGACCGGAAGCACCAGCCAGCTCC10.684931506849315No Hit
CTCTTATACACATCTCCGAGCCCACGAGAAATCTCGTATGCCGTCTTCTG10.684931506849315TruSeq Adapter, Index 12 (95% over 23bp)
GCGTGGGCGTGCCGTTCGGCCACACGCGGCCGCAGTGGGTGCTGCCTGTC10.684931506849315No Hit
GGCCGGCCGTGCACGCCCAGTTCGAGCGCCTGTTGGACCTGTCTCTTATA10.684931506849315No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTT10.684931506849315No Hit
CAGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCAC10.684931506849315No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT10.684931506849315No Hit
GTCTTCTTTTGAGAAGTGTCTGTTCATGTCCTTTGCCCACTTTTTGATGG10.684931506849315No Hit
ATCCCTGAGAATCCAAAATTCTCCGTGCCACCTATCACACCCCATCCTAA10.684931506849315No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA10.684931506849315No Hit
ATAAACCCTAGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTA10.684931506849315No Hit
GGTGGAGTGGGTTTGGGGCTAGGTTTAGCTCAGAGCGGTCAAGTTAAGTT10.684931506849315No Hit
GCCCTGGATCACCGAGCCGGGCGCATGGCTGCAGTACGACTTCGGCGATG10.684931506849315No Hit
GTGTACGCGCTTCAGGGCCCTGTTCAACTAAGCACTCTACTCTCAGTTTA10.684931506849315No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT10.684931506849315No Hit
GAGCAGAACCCAACCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGC10.684931506849315No Hit
TCTTATACACATCTCCGAGCCCATGAGACTTTAATGCATCTCGTATGCCG10.684931506849315No Hit
GCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTTCTGCTTG10.684931506849315TruSeq Adapter, Index 10 (96% over 28bp)
GCCCTCTCCCCGCCCAGCCGCCTTTCCTGCCCCGATCCCGGGTCGCCAGG10.684931506849315No Hit
GTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGG10.684931506849315No Hit
ACGCCGCCCCGCTCGACCTGCCGCTGACCGGCGCGGGGCGCACCCGGGAG10.684931506849315No Hit
GTCTCGAAGCGGATGCCCTTGACTCCCTCCATCTCCGCGGTCTCGTCACG10.684931506849315No Hit
TTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTGGGCCTAGCCCTA10.684931506849315No Hit
TCTAACAGCCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAAT10.684931506849315No Hit
GTACTATGTACTGTTAAAGATGGGTAGGTTTGTTGGTATCCTAGTGGGTG10.684931506849315No Hit
CTCTCTCCCAGGAGCCAGGTTTCAACCTCTTCCTGGTAATCTTTCATGTG10.684931506849315No Hit
AGCTGCTGCTGTGCCACTGGCGCCACCCCATCGCTGTCTCTTATACACAT10.684931506849315No Hit
CTCCCCCGCCCGCCTGCCTGAGGGGCCGCGCGTGGCCGTGTAGCTGTCTC10.684931506849315No Hit
GTCCCGGGTGGTCACGCCTGCGGCGGTGAGCTCGGTGTAGGCGCCCCTCA10.684931506849315No Hit
CCTCAACCCCGACATCATTACCGGGTTTTCCTCTTGTAAATATAGTTTAA10.684931506849315No Hit
CGTAGACCTGCCGGATGACGGCGTCGCACTCGTCCACCGTGACCATGAAG10.684931506849315No Hit
GATGAGCAAGAAGGATATAATTCCTACGCCCTCTCAGCCGATGAACAGTT10.684931506849315No Hit
ATACACATCTCCGAGCCCAGAGACCGGTTCCCATCTCGTATGCCGTCTTC10.684931506849315RNA PCR Primer, Index 27 (95% over 23bp)
TCCTCGGCCTGCGGCCGGAGTTCTTCGAGGACGCCGAGCTGGTGGAGGAC10.684931506849315No Hit
TCCCGCCGCTGAGCCGCATCCCGGACGAGTGCTACCCGGGCTGTCTCTTA10.684931506849315No Hit
CTCGCGTGCCGTGGCCAGGAGCTCGGCCAGTTCCGCGGCTGTCTCTTATA10.684931506849315No Hit
GTGTAGCCCACCGTCCGCAGGGTGATGAGCCGCTCCTCGCCGCCGATCGT10.684931506849315No Hit
TCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTTCTGCTTG10.684931506849315Illumina PCR Primer Index 3 (96% over 28bp)
ATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAG10.684931506849315No Hit
CTTAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC10.684931506849315RNA PCR Primer, Index 19 (95% over 21bp)
GCCCTGGGGCAGGGCGCGCCTCGCGCTCCCGGGAGCCTGTCTCTTAAACA10.684931506849315No Hit
GGTTTAATCCACCTCAACTGCCTGCTATGATGGATAAGATTGAGAGCTGT10.684931506849315No Hit
CCCATCGACTGCGGGGTCAGGACGATCGCCCGCGAGCCGAGCTCCCGCCG10.684931506849315No Hit
CGCCACTGCACTCCAGCCTGGGCGACAGAGTGAGACCTGTCTCTTATACA10.684931506849315No Hit
AACTAAGATTAGTATGGTAATTAGGAAGATGAGTAGATATTTGAAGAACT10.684931506849315No Hit
CTTTACACATCTCCGAGCCACGAGACTTTAATGCATCTCGTATGCCGTCT10.684931506849315No Hit
CTATCAAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAGC10.684931506849315No Hit
CCTTCATGATCCAGGAGAGCCAGATGTAGGCGAGCACGATCGGCATGAGC10.684931506849315No Hit
CCAGTGGAATTAAGCTGCCTTGACAAAGTCATGCCTTCTTTCTGACAGCA10.684931506849315No Hit
CCCTAGGAGGCCTGCCCCCGCTAACCGGCTTTTTGCCCAAATGGGCCATT10.684931506849315No Hit
GCCTCCCAAAGTGCTGGGATTACAGGCTTGAGCCACCACGCCCCTGTCTC10.684931506849315No Hit
GCCTATGAGTGACTACAAAAAGGATTAGACTGAACCGAATTGGTATATAG10.684931506849315No Hit
GTGAGCCGGTCCCCGCGCCAGTCGGCGGTGTCCTCGATGCGCCGCAGGCC10.684931506849315No Hit
TTGCATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATTCTC10.684931506849315No Hit
TCTGCAGGTGCTTACACATGCACCTGCACCTAATCTGTGAGAAGAGTTAA10.684931506849315No Hit
CATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGCTTC10.684931506849315No Hit
CGCCCGCGCCGCGGCCCGCTCCCTACCGTGGTGCCCATGCTGTCTCTTAT10.684931506849315No Hit
GCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAA10.684931506849315No Hit
GTTCCTAGAATGGTGCGGTGACTCTGACGGACCCCACCCCCCGCACCACC10.684931506849315No Hit
GCCCACGGCGCCCTCGTCGCCGACAACGCCCTCACCGGCGCCGGCCTCGA10.684931506849315No Hit
CGGGGTGCGCGGGTGCCCGCTGTGCCGATGGCCCGCGGATCCGACGGGCG10.684931506849315No Hit
ACTGCGACATAGGGTGCTCCGGCTCCAGCGTCTCGCAATGCTATCGCGTG10.684931506849315No Hit
TTCCTAGGGGGTTGTTTGATCCCGTTTCGTGCAAGAATAGGAGGTGGAGT10.684931506849315No Hit
ATAAAAGAGTTACTTTGATAGAGTAAATAATAGGAGCTTAAACCCCCTTA10.684931506849315No Hit
AGCGTGGTAAGGGCGATGAGTGTGGGGAGGAATGGGGTGGGTTTTGTATG10.684931506849315No Hit
CCTCCCCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTTCT10.684931506849315RNA PCR Primer, Index 34 (95% over 23bp)
TCTCCACTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTTCT10.684931506849315Illumina PCR Primer Index 6 (95% over 24bp)
CAACCCTAACATAACCATTCTTAATTTAACTATTTATATTATCCTAACTA10.684931506849315No Hit
CATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATACCGCCTTCTTCT10.684931506849315No Hit
CTGACTAGTTCCCCTAATAATCGGTGCCCCCGATATGGCGTTTCCCCGCA10.684931506849315No Hit
ATCCGACTCGACGCGAGGCCGGACACGACGAAAGGGGGGCGCCCCGCCGA10.684931506849315No Hit
CACCTGTAGTACATAAAAACCCAATCCACATCAAAACCCCCTCCCCATGC10.684931506849315No Hit
CCACCACGCCGGAGTCCACGCGCACGCCCGGGCCGCTGGGCATGCGGAAC10.684931506849315No Hit
TCATCCCTCTTATACACATCTCCGAGCCCAGAGACACCAGCTAATCTCGT10.684931506849315No Hit
GTCCAGGCCGTCGAGGATGCGGTCGAGGTCCGGCAGCTGTCTCTTATACA10.684931506849315No Hit
GCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCATTAAGTGTTCCCCTT10.684931506849315No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC10.684931506849315No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers