FastQCFastQC Report
Wed 25 May 2016
SRR1780618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101886
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT150.002220058669.9921534
CCGTCTA150.002220058669.992159
GGAACAC150.002220058669.9921567
TACGGGG252.3819898E-455.99371330
TTACGGG252.3819898E-455.99371329
ACGTAGA252.3819898E-455.9937132
GCGCTTT252.3819898E-455.99371341
GCCTTAT200.00693702952.49410657
ATAGGCG200.00693702952.49410632
TTTCGGG200.00693702952.49410657
TGTATTG200.00693702952.49410627
CGTCTAA200.00693702952.49410610
TGAGTCC200.00693702952.49410626
TACGGAG200.00693702952.49410625
GGCCAAT200.00693702952.49410631
TTTGGCC200.00693702952.49410662
GTTTTTA200.00693702952.49410649
CGATAGA200.00693702952.49410637
GAGGCTC200.00693702952.49410629
GCCTGTG305.8573E-446.6614347
Produced by FastQC (version 0.11.5)