FastQCFastQC Report
Wed 25 May 2016
SRR1780618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101886
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT13601.3348251967885676No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5850.5741711324421412RNA PCR Primer, Index 48 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC1710.16783463871385668No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1500.1472233672928567No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1100.10796380268142827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA150.00221907170.0000143
CGCGAGA150.00221907170.0000132
CCTCGTG150.00221907170.000017
AATATCG252.822433E-670.0000136
ATCGTAT252.822433E-670.0000139
ATATCGT252.822433E-670.0000137
ATGGTGC150.00221907170.0000163
TATACAG150.00221907170.000012
GCTCGCG150.00221907170.0000129
GGCTCGC150.00221907170.0000128
CGTGGAG200.00693395152.510
AGCCGCT200.00693395152.570
GATCGGC200.00693395152.535
ACGTTGG200.00693395152.516
CCTAGAT200.00693395152.542
GTTTAGT200.00693395152.553
CGTTGGG200.00693395152.517
TCGTGGA200.00693395152.59
CTTTGCG200.00693395152.526
GTCCGTA200.00693395152.511
Produced by FastQC (version 0.11.5)