##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780618_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 101886 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.9074161317551 34.0 34.0 34.0 31.0 34.0 2 33.30061048622971 34.0 34.0 34.0 33.0 34.0 3 33.49715368156567 34.0 34.0 34.0 34.0 34.0 4 33.54816167088707 34.0 34.0 34.0 34.0 34.0 5 33.56380660738473 34.0 34.0 34.0 34.0 34.0 6 37.26925190899633 38.0 38.0 38.0 36.0 38.0 7 36.96053432267436 38.0 37.0 38.0 35.0 38.0 8 36.9481381151483 38.0 37.0 38.0 35.0 38.0 9 36.761851481067076 38.0 37.0 38.0 34.0 38.0 10-11 36.509000255187175 38.0 37.0 38.0 34.0 38.0 12-13 36.39972125709126 38.0 37.0 38.0 34.0 38.0 14-15 36.648587637163104 38.0 37.0 38.0 34.0 38.0 16-17 36.7680937518403 38.0 37.0 38.0 34.5 38.0 18-19 36.77336434838938 38.0 37.0 38.0 34.0 38.0 20-21 36.53650648764305 38.0 37.0 38.0 34.0 38.0 22-23 36.60352747188034 38.0 37.0 38.0 34.0 38.0 24-25 36.001786310189814 38.0 36.5 38.0 32.5 38.0 26-27 36.195242722258214 38.0 37.0 38.0 34.0 38.0 28-29 36.08713169620949 38.0 37.0 38.0 33.5 38.0 30-31 35.82535382682606 38.0 36.5 38.0 32.5 38.0 32-33 36.28895530298569 38.0 37.0 38.0 34.0 38.0 34-35 36.32571697779872 38.0 37.0 38.0 34.0 38.0 36-37 36.44258288675579 38.0 37.0 38.0 34.0 38.0 38-39 36.08900634041969 38.0 37.0 38.0 32.5 38.0 40-41 35.64398445321241 38.0 36.0 38.0 31.5 38.0 42-43 36.03253145672615 38.0 37.0 38.0 32.5 38.0 44-45 36.21372416229904 38.0 37.0 38.0 34.0 38.0 46-47 36.4827208841254 38.0 37.0 38.0 34.0 38.0 48-49 36.29203717880769 38.0 37.0 38.0 34.0 38.0 50-51 36.441213709439964 38.0 37.0 38.0 34.0 38.0 52-53 36.36961898592544 38.0 37.0 38.0 34.0 38.0 54-55 36.54589443103076 38.0 37.0 38.0 34.0 38.0 56-57 36.580168030936534 38.0 37.0 38.0 34.0 38.0 58-59 36.62740219460966 38.0 37.0 38.0 34.0 38.0 60-61 36.32804801444752 38.0 37.0 38.0 34.0 38.0 62-63 36.582499067585346 38.0 37.0 38.0 34.0 38.0 64-65 36.572213061657145 38.0 37.0 38.0 34.0 38.0 66-67 36.03275719922266 38.0 37.0 38.0 33.0 38.0 68-69 35.5193009834521 38.0 36.5 38.0 31.0 38.0 70-71 35.516538091592565 38.0 36.0 38.0 31.0 38.0 72-73 35.200773413422844 38.0 36.0 38.0 29.0 38.0 74-75 35.75528531888581 38.0 37.0 38.0 32.0 38.0 76 35.20519011444163 38.0 36.0 38.0 28.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.538463408160105 1101 2 0.47289268329662093 1101 3 -0.10063664112804815 1101 4 0.023676919318049272 1101 5 -0.013423383413730505 1101 6 -1.0399697944526523 1101 7 -0.011868494833116472 1101 8 0.22478479253363304 1101 9 -0.08240428510107733 1101 10-11 -0.009602801826183338 1101 12-13 0.17275427485447636 1101 14-15 0.27930977795016787 1101 16-17 -0.31563908838283083 1101 18-19 -0.7141204858178014 1101 20-21 -0.09572897888535437 1101 22-23 0.2774779230328832 1101 24-25 -2.4768261678620007 1101 26-27 -0.8924278295960733 1101 28-29 0.22792176219429194 1101 30-31 0.6352650769077428 1101 32-33 -0.17726725837623292 1101 34-35 -0.001267303994389124 1101 36-37 -0.3832953296200685 1101 38-39 -0.40659875984940896 1101 40-41 0.4647056139636092 1101 42-43 -0.11749825938410652 1101 44-45 -0.21442793284744965 1101 46-47 -0.01871782717142878 1101 48-49 -1.8951655757865282 1101 50-51 -1.4602028689431208 1101 52-53 -1.451083163928999 1101 54-55 -1.5309346487655802 1101 56-57 -1.291786964812914 1101 58-59 -1.2629383152620193 1101 60-61 -1.489587803999278 1101 62-63 -1.0218080889993004 1101 64-65 -1.3952613464826058 1101 66-67 -1.652672228809557 1101 68-69 -1.34451061545181 1101 70-71 -1.0314701223396838 1101 72-73 -1.6772509737631154 1101 74-75 -1.2349552465897276 1101 76 -1.3566905072573334 1102 1 -0.7624639781246785 1102 2 0.21830306749950523 1102 3 0.2784246168367517 1102 4 0.33889362488867647 1102 5 0.2299366569622734 1102 6 0.3072007310662457 1102 7 -0.03987934588352715 1102 8 0.44688434461237136 1102 9 0.526484743982266 1102 10-11 0.6425642538800886 1102 12-13 0.6282618138110152 1102 14-15 -0.24649301051744033 1102 16-17 0.3368276329596753 1102 18-19 -0.11434760082632067 1102 20-21 0.4990126777924573 1102 22-23 0.583058012617137 1102 24-25 -4.025813613449031 1102 26-27 -0.624574066426554 1102 28-29 0.08897153823365045 1102 30-31 1.3672535949823157 1102 32-33 0.23166593195875862 1102 34-35 0.7433544864289203 1102 36-37 -2.5850964778126126 1102 38-39 0.11989799114421373 1102 40-41 0.6933347225371946 1102 42-43 0.6514753069524062 1102 44-45 0.9021104558977413 1102 46-47 1.004608776828313 1102 48-49 -3.0355194966747945 1102 50-51 -0.5645495978561215 1102 52-53 -0.39452521805807805 1102 54-55 0.46414452604988554 1102 56-57 0.654147045659613 1102 58-59 -0.3288805271098525 1102 60-61 -5.520374505153779 1102 62-63 -2.1121178486359184 1102 64-65 0.06824253339878794 1102 66-67 1.138397735230562 1102 68-69 -0.30432387644481196 1102 70-71 -1.4931602365280625 1102 72-73 -5.987673029153946 1102 74-75 -2.175621450614706 1102 76 -0.46141576118452576 1103 1 0.5735076302856612 1103 2 0.47724240056499667 1103 3 0.18524127680728952 1103 4 0.42231400790729623 1103 5 0.2386282332311893 1103 6 0.21673601966943323 1103 7 0.6948735669328698 1103 8 0.3720082230106527 1103 9 0.2721121713292547 1103 10-11 0.025013698101325588 1103 12-13 -0.45878117534125806 1103 14-15 -0.17625061062457803 1103 16-17 -0.006482933532893753 1103 18-19 0.5806236058162426 1103 20-21 -0.19452555710777375 1103 22-23 0.3511911316743266 1103 24-25 -2.821193283999449 1103 26-27 -0.48047335663681423 1103 28-29 -0.2203963471286059 1103 30-31 0.7028234356144267 1103 32-33 0.980634727994655 1103 34-35 0.4418746417791297 1103 36-37 -1.0562763358546619 1103 38-39 -0.1998421990258663 1103 40-41 -0.42358639705567924 1103 42-43 -0.4035936631828605 1103 44-45 0.9828389948022505 1103 46-47 0.7355448957515804 1103 48-49 -2.179854571001833 1103 50-51 -0.5815309826157247 1103 52-53 0.3573697866292207 1103 54-55 -0.4769764060513566 1103 56-57 0.3008257653596189 1103 58-59 -0.08599181678442136 1103 60-61 -4.337630431228511 1103 62-63 -1.3074975191863416 1103 64-65 0.19593772557770706 1103 66-67 -0.31904166479622376 1103 68-69 0.2345672444872875 1103 70-71 0.36205604845921613 1103 72-73 -4.844657092365409 1103 74-75 -2.112068484229276 1103 76 -2.674205368791334 1104 1 0.005405556920429433 1104 2 0.0404959890817409 1104 3 0.15870220184715578 1104 4 -0.20971151043401193 1104 5 -0.24449776357901953 1104 6 -0.07749045560967716 1104 7 0.3593711745883681 1104 8 0.5533798338559492 1104 9 0.8985874504361178 1104 10-11 -0.718941644801383 1104 12-13 0.09308485336687511 1104 14-15 0.3467477944790929 1104 16-17 0.2748567793857646 1104 18-19 0.4019952166615397 1104 20-21 -0.21324964004238467 1104 22-23 -0.05078653977702885 1104 24-25 0.23325647676610117 1104 26-27 0.4241837406518627 1104 28-29 0.787673828309984 1104 30-31 0.47749648186642446 1104 32-33 0.17339389864856258 1104 34-35 -0.44217639490348404 1104 36-37 0.805382356330341 1104 38-39 0.9277814054398448 1104 40-41 1.3297469362776582 1104 42-43 -0.01667181310311605 1104 44-45 0.4717704142599928 1104 46-47 0.5691334124979903 1104 48-49 -0.2569837576047078 1104 50-51 0.22558225502383067 1104 52-53 -0.13108316392899866 1104 54-55 -0.432670185955665 1104 56-57 -0.39732415489555706 1104 58-59 -0.48178129046862495 1104 60-61 0.298924592694938 1104 62-63 0.2978613324883028 1104 64-65 0.31663947996367625 1104 66-67 -0.23556479079303472 1104 68-69 1.2510265746308278 1104 70-71 1.1826621090652765 1104 72-73 1.0698564642534123 1104 74-75 0.12595384431936907 1104 76 -0.028591333703609223 1105 1 0.8386875291854565 1105 2 0.39285346211718064 1105 3 0.3218454992277415 1105 4 0.13013440636105855 1105 5 -0.2025254985559073 1105 6 0.2910149373024993 1105 7 0.7771215597963845 1105 8 -0.14960938031970272 1105 9 0.4982792840262533 1105 10-11 0.44226020419707623 1105 12-13 -0.23763933769630086 1105 14-15 0.1921407066516636 1105 16-17 0.16514953747513772 1105 18-19 0.32341278214690306 1105 20-21 0.21953926596686557 1105 22-23 0.08015336777304327 1105 24-25 0.12126880342249535 1105 26-27 0.2304395187720445 1105 28-29 0.6941145062760867 1105 30-31 1.033212968769348 1105 32-33 0.7111134672155899 1105 34-35 -0.7255354087709733 1105 36-37 0.2676011544813477 1105 38-39 0.23881376291288348 1105 40-41 0.2538147328878253 1105 42-43 0.28995376470890477 1105 44-45 0.5406147902230032 1105 46-47 0.6720301767506882 1105 48-49 -0.3655816004398389 1105 50-51 0.4474312534829963 1105 52-53 0.2805038930817858 1105 54-55 0.5478228803001102 1105 56-57 0.5092706062754786 1105 58-59 0.31008311631102714 1105 60-61 0.6910047159615047 1105 62-63 -0.29893373684913627 1105 64-65 0.4579954121670582 1105 66-67 0.331954469607588 1105 68-69 0.137867868929753 1105 70-71 0.8309826021315274 1105 72-73 1.0248641684136572 1105 74-75 0.27621578576775363 1105 76 0.3680188357879146 1106 1 -0.4415339577481632 1106 2 0.33717110679271656 1106 3 0.40351205335023366 1106 4 0.2679560013370832 1106 5 0.10985210965532133 1106 6 0.21792424920742093 1106 7 -0.6481333527567941 1106 8 0.3671574506614519 1106 9 -0.437232904507745 1106 10-11 0.3166541538223058 1106 12-13 1.0770544296073652 1106 14-15 -0.34595774699138815 1106 16-17 0.23874667435135422 1106 18-19 0.5255736302797942 1106 20-21 -0.0335973401510401 1106 22-23 0.22231016431569373 1106 24-25 -0.04824297995247662 1106 26-27 1.042452483860842 1106 28-29 0.4315782107801098 1106 30-31 0.9224222334057188 1106 32-33 0.5186021565261427 1106 34-35 1.004074963300063 1106 36-37 0.8012968800536342 1106 38-39 0.3289548933065589 1106 40-41 1.1782728326919951 1106 42-43 0.8867327504608156 1106 44-45 0.787424162901786 1106 46-47 0.8845698485718785 1106 48-49 0.9204809291030145 1106 50-51 0.1923768946471469 1106 52-53 0.40875022976894115 1106 54-55 -0.16491937103352683 1106 56-57 0.23480913014608973 1106 58-59 0.6253103429975084 1106 60-61 0.7128833033070308 1106 62-63 0.5726240996016685 1106 64-65 -0.985511914458634 1106 66-67 0.011439917645937214 1106 68-69 1.0138081755000812 1106 70-71 0.2802499395614717 1106 72-73 0.3938695030074868 1106 74-75 -0.21584984999428514 1106 76 0.5801736065354319 1107 1 0.4906808450126192 1107 2 -0.2664566357582103 1107 3 0.16647428891501903 1107 4 0.12625519891297898 1107 5 0.09008610325837196 1107 6 0.32416127294346353 1107 7 0.6972456944347201 1107 8 0.2533030092720807 1107 9 -0.27356363791215443 1107 10-11 0.456320839193495 1107 12-13 -0.15974485213888556 1107 14-15 -0.049551821397990636 1107 16-17 0.4362524259926843 1107 18-19 0.43938318081006855 1107 20-21 0.6816543292277899 1107 22-23 -0.17364825552606789 1107 24-25 0.20749463297608628 1107 26-27 -0.07121958841344167 1107 28-29 0.7228146733804053 1107 30-31 0.26093438199445984 1107 32-33 0.8776448589558612 1107 34-35 0.11172885477641614 1107 36-37 0.818180051592833 1107 38-39 0.322550931688248 1107 40-41 0.18122580951709466 1107 42-43 0.7162667272297938 1107 44-45 0.3037742554891807 1107 46-47 0.7520015302956864 1107 48-49 0.7852121450841523 1107 50-51 0.8580662499021869 1107 52-53 0.3863048002195342 1107 54-55 0.5127011328663613 1107 56-57 0.6383288540673107 1107 58-59 0.37709194896799403 1107 60-61 0.9106275376373176 1107 62-63 0.5983030738455426 1107 64-65 0.7354422968650809 1107 66-67 -0.09493739002478918 1107 68-69 0.9731328486385138 1107 70-71 0.07285417052494836 1107 72-73 0.6953046076593026 1107 74-75 -0.7119078714296663 1107 76 0.28901430009920404 1108 1 -0.3932686855038128 1108 2 0.22249093857669067 1108 3 -0.005767495122540822 1108 4 0.38496020673770204 1108 5 0.09473203440077782 1108 6 -0.28509146571068555 1108 7 -0.08297731026011235 1108 8 -0.42959996412384527 1108 9 -0.39137467077600263 1108 10-11 0.5355217390370015 1108 12-13 0.9135141462961727 1108 14-15 0.4350432490245453 1108 16-17 -0.21901948324049414 1108 18-19 0.3196404186817432 1108 20-21 0.8384422791495396 1108 22-23 0.14689654103104743 1108 24-25 1.210257739392361 1108 26-27 -0.4637518654087387 1108 28-29 -0.60447894946779 1108 30-31 -0.03332422520428224 1108 32-33 0.6915630905677546 1108 34-35 0.7293198172177284 1108 36-37 1.0981096290576176 1108 38-39 1.1228003448337915 1108 40-41 1.1465524918332122 1108 42-43 -0.35366676259805985 1108 44-45 0.894516626381197 1108 46-47 0.8708884630030411 1108 48-49 0.6908508383548906 1108 50-51 0.6509799822965547 1108 52-53 0.06343703809120882 1108 54-55 0.05182476353928678 1108 56-57 0.22599023904383841 1108 58-59 0.5891909317535919 1108 60-61 0.9921758553212001 1108 62-63 0.8670090597610312 1108 64-65 0.9679589244081157 1108 66-67 0.8443720778938371 1108 68-69 1.3847134433176933 1108 70-71 -0.11572172931856528 1108 72-73 0.5041367672837183 1108 74-75 -0.32886307487255095 1108 76 0.9380753329630522 1109 1 -1.0514516751945315 1109 2 0.11732198095928226 1109 3 0.12674968700804357 1109 4 0.2281016729461811 1109 5 -0.059942936962336546 1109 6 -0.2645445012204206 1109 7 -0.22966975012266033 1109 8 0.3935672752805033 1109 9 0.49490110241987395 1109 10-11 0.6540605420152374 1109 12-13 0.4911916949788733 1109 14-15 0.6522898125984256 1109 16-17 0.6209361295888343 1109 18-19 0.4953098058524148 1109 20-21 0.7813832871678628 1109 22-23 0.44337778387183135 1109 24-25 0.1414039306867494 1109 26-27 0.48389633046754454 1109 28-29 -0.38442239162127834 1109 30-31 0.5910672410041897 1109 32-33 0.7194482566616784 1109 34-35 -0.27438507968948755 1109 36-37 0.7338484606727391 1109 38-39 0.813360911844093 1109 40-41 0.7203998572398547 1109 42-43 1.1763303737135118 1109 44-45 0.9574998738038829 1109 46-47 0.7044723690740611 1109 48-49 0.522985002157867 1109 50-51 -0.25188728574467945 1109 52-53 -0.2708269939821051 1109 54-55 -0.38388543119152274 1109 56-57 -0.17376276782901812 1109 58-59 -0.07944452070918118 1109 60-61 0.698343524278819 1109 62-63 0.3420412762558769 1109 64-65 0.8151274524722609 1109 66-67 -0.007611026344420679 1109 68-69 -0.3668615853191852 1109 70-71 -0.06646628831054358 1109 72-73 -0.4171475969774505 1109 74-75 -0.5961704918255819 1109 76 -0.07051573232904218 1110 1 -0.5823857131813099 1110 2 -0.008982186172609374 1110 3 0.10712359994364817 1110 4 -0.04211059149735519 1110 5 0.24408773034943465 1110 6 0.05453448695678276 1110 7 0.6096370098361561 1110 8 0.7665993940791225 1110 9 0.9624535482372352 1110 10-11 -0.24780281972754636 1110 12-13 0.616708089336683 1110 14-15 0.8990549818765317 1110 16-17 0.6393464413483585 1110 18-19 -0.12298016896366448 1110 20-21 0.7610004420055319 1110 22-23 -0.5709571994422831 1110 24-25 -1.1292115245466618 1110 26-27 1.1037308361556342 1110 28-29 0.2777099293930192 1110 30-31 0.27151682974958646 1110 32-33 0.486075224542887 1110 34-35 0.7700651541611734 1110 36-37 1.0782540333897543 1110 38-39 0.7455628297916661 1110 40-41 -1.2299642550580856 1110 42-43 0.2600265787577314 1110 44-45 0.9148422882343823 1110 46-47 0.7279256671747234 1110 48-49 1.2718839811547298 1110 50-51 -0.1365312992918959 1110 52-53 0.6037248439476031 1110 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2116 34-35 -0.9448699975970882 2116 36-37 0.3499614809094709 2116 38-39 -3.4650468773884384 2116 40-41 -0.836549360018644 2116 42-43 -1.269870466301768 2116 44-45 0.14463236712194316 2116 46-47 -0.5492504258858517 2116 48-49 -0.3439871246080699 2116 50-51 -0.5364042769627062 2116 52-53 0.15649259364676027 2116 54-55 -0.03613819942367513 2116 56-57 0.05180042422902176 2116 58-59 -1.8221516608390047 2116 60-61 -1.2632639594936066 2116 62-63 0.3192081338351045 2116 64-65 -1.321508668184471 2116 66-67 -0.5461371813654239 2116 68-69 -0.9643606307563743 2116 70-71 1.0287227151258804 2116 72-73 0.4006645450614954 2116 74-75 0.29231748068300334 2116 76 -0.09896170407398586 2117 1 0.6866760936126539 2117 2 0.426724539647644 2117 3 0.08936302696581322 2117 4 -0.08734568348987182 2117 5 0.19166141860426222 2117 6 0.1617793508890486 2117 7 0.5392616455631796 2117 8 0.3252527801867302 2117 9 -1.4226246707760026 2117 10-11 -0.45416617930302294 2117 12-13 -0.9794744748551025 2117 14-15 0.00394385143418674 2117 16-17 -0.7814776492378215 2117 18-19 -0.8191438852004538 2117 20-21 -0.15009520411685884 2117 22-23 0.11642047008057688 2117 24-25 -1.691453271317144 2117 26-27 -1.7406720461316354 2117 28-29 -1.4116032801237495 2117 30-31 -0.5858258985643943 2117 32-33 -1.8545798143853887 2117 34-35 0.44424200597274677 2117 36-37 -1.154902419135155 2117 38-39 0.10516731270527657 2117 40-41 -0.11595587496732662 2117 42-43 -0.10860317491398774 2117 44-45 -0.6346625904862719 2117 46-47 -0.5887417244132891 2117 48-49 -0.7949465177361432 2117 50-51 -0.2946148972215141 2117 52-53 0.3848058466588071 2117 54-55 -0.23616051089177148 2117 56-57 -0.01792291356660769 2117 58-59 -1.6640302864525651 2117 60-61 -0.4734156774766589 2117 62-63 -0.6371952575080471 2117 64-65 -1.3627179497552007 2117 66-67 -0.715659715948199 2117 68-69 0.08434530409414265 2117 70-71 -0.6511969635889798 2117 72-73 0.438750221098978 2117 74-75 0.3949279224500728 2117 76 -0.08802908470762816 2118 1 0.01358083487988182 2118 2 0.2848910185127451 2118 3 -0.4849341617892051 2118 4 0.15769202128606707 2118 5 0.3022909872385142 2118 6 0.35544610423335854 2118 7 0.7082397161187828 2118 8 0.5303320902326689 2118 9 -1.2991234717352356 2118 10-11 -0.5921136526496511 2118 12-13 -0.8976868928724926 2118 14-15 0.57330763751375 2118 16-17 -2.4339725208104355 2118 18-19 -0.21357051257325566 2118 20-21 -0.3029945713700428 2118 22-23 0.46852673687437374 2118 24-25 0.8479850575378478 2118 26-27 -2.1563852586941152 2118 28-29 -0.11930458895619722 2118 30-31 -0.4229874945887815 2118 32-33 0.1740021393287492 2118 34-35 0.21586807860861512 2118 36-37 -2.5223939680646907 2118 38-39 0.26065256305921025 2118 40-41 0.05614981397550167 2118 42-43 0.6405878337248794 2118 44-45 -1.7275232417243132 2118 46-47 0.08344341903821118 2118 48-49 0.1242915857569713 2118 50-51 -0.031461564466034986 2118 52-53 -0.9572963751781742 2118 54-55 -0.5418003363807813 2118 56-57 0.309660802593001 2118 58-59 -0.790630211331937 2118 60-61 -0.49936840926712733 2118 62-63 -0.5332832064260202 2118 64-65 0.2698049475895701 2118 66-67 0.32671120659087904 2118 68-69 -0.5324528236687129 2118 70-71 0.47740377028175374 2118 72-73 1.2972272948616492 2118 74-75 0.5984325885966726 2118 76 0.6203295296057405 2119 1 0.437399919147353 2119 2 -0.8083553146532836 2119 3 -0.04307369667292704 2119 4 -0.19853462530363686 2119 5 0.029244308302587285 2119 6 -0.2145165303101706 2119 7 -0.5795858373918961 2119 8 0.1385524906565152 2119 9 0.05411951527050718 2119 10-11 -0.09993530230666892 2119 12-13 -0.263003941559127 2119 14-15 -0.03661372771964011 2119 16-17 -0.3189064341448855 2119 18-19 -0.31484763041386543 2119 20-21 -1.0431791678788827 2119 22-23 -0.13705856232812863 2119 24-25 0.9713607755680727 2119 26-27 0.6577355376920337 2119 28-29 -0.08069826471326991 2119 30-31 1.2613301675605726 2119 32-33 0.30921489935327884 2119 34-35 0.3369990807836203 2119 36-37 0.3616755205304827 2119 38-39 -0.9567781435383012 2119 40-41 -0.45963290868358797 2119 42-43 0.48241204877145094 2119 44-45 0.562031161264926 2119 46-47 0.2789149487911544 2119 48-49 -0.10376317973296523 2119 50-51 0.6930100210562458 2119 52-53 0.4402135238793221 2119 54-55 0.398400990929467 2119 56-57 -0.920206466382524 2119 58-59 0.1269008800740039 2119 60-61 0.509819589171336 2119 62-63 0.057537160536227816 2119 64-65 -0.15708145026888332 2119 66-67 0.4345691063176105 2119 68-69 0.5846488438345006 2119 70-71 -0.05499821510667857 2119 72-73 0.9554449068185988 2119 74-75 -0.011558488309241 2119 76 -0.16269986083539578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 5.0 22 8.0 23 17.0 24 34.0 25 71.0 26 117.0 27 274.0 28 464.0 29 727.0 30 1219.0 31 2029.0 32 3237.0 33 4993.0 34 7817.0 35 12609.0 36 23011.0 37 45253.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.941424729599746 21.986337671515223 11.383310759083681 28.688926839801347 2 18.516773648980234 18.781775710107375 37.013917515654754 25.687533125257637 3 17.729619378521093 23.787370198064504 29.88830653868049 28.594703884733917 4 13.641717213356102 18.316549869461948 37.02765836326875 31.014074553913197 5 13.465049172604676 35.68203678621204 36.54869167500932 14.30422236617396 6 29.303339025970203 38.19072296488232 16.90320554345052 15.602732465696956 7 28.385646703178065 28.56918516773649 22.45450797950651 20.59066014957894 8 23.858037414365075 35.118662034038046 20.051822625287087 20.9714779263098 9 23.051253361600217 15.428027403176097 21.84696621714465 39.673753018079026 10-11 24.417976954635574 25.589384213729073 27.54892723239699 22.443711599238362 12-13 24.435153014153073 22.759751094360364 26.94187621459278 25.863219676893785 14-15 21.59226979172801 24.885656518069215 23.53561823999372 29.98645545020906 16-17 22.75729737157215 28.10837602810985 25.571226665096287 23.563099935221718 18-19 22.536953065190506 27.267239856309995 26.474687395716785 23.721119682782717 20-21 21.36358282786644 26.81820858606678 26.497261645368354 25.32094694069843 22-23 22.36224800266965 27.900300335669275 26.64644799089178 23.091003670769293 24-25 22.51388807098129 28.445026794652843 26.418742516145493 22.622342618220365 26-27 21.882299825294936 28.278173645054277 27.35802759947392 22.481498930176862 28-29 21.399407180574368 26.397149755609213 29.070235361089846 23.133207702726576 30-31 21.40873132716958 26.47321516204385 29.2311995759967 22.886853934789862 32-33 22.00645819837858 27.14749818424514 26.85795889523585 23.98808472214043 34-35 21.63839978014644 26.76962487486014 28.530907092240348 23.061068252753078 36-37 21.873957167815007 28.154506016528273 27.15780381995564 22.813732995701077 38-39 21.48872269006537 26.971320888051352 27.98176393223799 23.55819248964529 40-41 21.58883457982451 25.674283022201283 28.786585006772274 23.950297391201932 42-43 22.120801680309366 26.0546100543745 28.788547985002843 23.03604028031329 44-45 20.615688122018728 25.96235007753764 29.262607227685844 24.15935457275779 46-47 21.870031211353865 24.992148087077716 29.130106197122274 24.007714504446145 48-49 20.885597628722298 25.153603046542212 29.6090728853817 24.351726439353786 50-51 19.634689751290658 25.545217203541213 30.150854877019412 24.669238168148716 52-53 19.55567987751016 25.92456274659914 30.993954027049842 23.525803348840864 54-55 18.55946842549516 26.385371886225784 30.159688279056983 24.895471409222072 56-57 19.616532202657872 26.12036982509864 28.997605166558703 25.265492805684787 58-59 18.87060047504073 26.472233672928567 29.804389219323557 24.852776632707144 60-61 19.477651492844945 27.277545492020494 28.333137035510276 24.91166597962429 62-63 18.19680819739709 28.56918516773649 27.47482480419292 25.7591818306735 64-65 19.087509569518872 28.920558271008773 26.657735115717568 25.334197043754784 66-67 18.794044324048446 29.085939186934418 25.704709184775144 26.415307304242 68-69 18.61737628329702 28.851363288381133 25.97069273501757 26.560567693304282 70-71 19.137565514398442 28.243330781461633 25.868127122470213 26.75097658166971 72-73 19.31030759868873 27.56021435722278 25.777830123863925 27.351647920224565 74-75 19.286751859921875 27.408574288911137 26.08650845062128 27.218165400545708 76 19.465873623461516 27.245156351216064 26.686689044618493 26.602280980703924 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 1.5 11 2.0 12 1.0 13 0.5 14 0.5 15 1.0 16 2.5 17 4.0 18 4.0 19 12.0 20 31.5 21 43.0 22 50.0 23 80.5 24 116.5 25 129.0 26 214.5 27 323.0 28 425.5 29 505.0 30 629.5 31 903.0 32 1284.5 33 1517.0 34 1682.5 35 2189.5 36 2708.0 37 2885.0 38 3300.5 39 4532.0 40 5975.0 41 6898.0 42 7194.0 43 7181.5 44 7351.0 45 7488.5 46 7444.0 47 7388.5 48 7078.0 49 6462.0 50 6101.0 51 5706.0 52 4736.0 53 3885.5 54 3610.0 55 3328.5 56 2671.5 57 2021.5 58 1747.0 59 1449.0 60 1036.0 61 795.0 62 669.0 63 595.0 64 450.5 65 333.5 66 216.0 67 145.0 68 136.5 69 97.5 70 53.5 71 40.0 72 29.0 73 16.5 74 8.5 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 101886.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.57911783758318 #Duplication Level Percentage of deduplicated Percentage of total 1 56.365880490488074 20.618141844806942 2 14.988328101102793 10.965196395971969 3 9.128229896160347 10.017077910605972 4 5.766186374735034 8.436880434995976 5 3.429123400144893 6.271715446675696 6 2.339746169738925 5.135151051174843 7 1.5723523571869382 4.026068350901989 8 1.1108427915962327 3.2506919498262765 9 0.877404813652097 2.8885224662858486 >10 4.365558507070219 25.10354710166264 >50 0.04293112238053074 0.9549889091729973 >100 0.008049585446349513 0.4230218086881416 >500 0.0026831951487831713 0.5741711324421412 >1k 0.0026831951487831713 1.3348251967885676 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT 1360 1.3348251967885676 No Hit ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT 585 0.5741711324421412 RNA PCR Primer, Index 48 (95% over 23bp) CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC 171 0.16783463871385668 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 150 0.1472233672928567 No Hit TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG 110 0.10796380268142827 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010796380268142827 0.0 2 0.0 0.0 0.0 0.014722336729285673 0.0 3 0.0 0.0 0.0 0.017666804075142806 0.0 4 0.0 0.0 0.0 0.024537227882142788 0.0 5 0.0 0.0 0.0 0.029444673458571345 0.0 6 0.0 0.0 0.0 0.0343521190349999 0.0 7 0.0 0.0 0.0 0.036315097265571326 0.0 8 0.0 0.0 0.0 0.03729658638085703 0.0 9 0.0 0.0 0.0 0.05201892311014271 0.0 10 0.0 0.0 0.0 0.06477828160885696 0.0 11 0.0 0.0 0.0 0.06968572718528551 0.0 12 0.0 0.0 0.0 0.06968572718528551 0.0 13 0.0 0.0 0.0 0.07361168364642837 0.0 14 0.0 0.0 0.0 0.0775376401075712 0.0 15 0.0 0.0 0.0 0.0775376401075712 0.0 16 0.0 0.0 0.0 0.08146359656871405 0.0 17 0.0 0.0 0.0 0.08637104214514262 0.0 18 0.0 0.0 0.0 0.08735253126042831 0.0 19 0.0 0.0 0.0 0.0942229550674283 0.0 20 0.0 0.0 0.0 0.09618593329799972 0.0 21 0.0 0.0 0.0 0.09716742241328544 0.0 22 0.0 0.0 0.0 0.11287124825785683 0.0 23 0.0 0.0 0.0 0.11287124825785683 0.0 24 0.0 0.0 0.0 0.11287124825785683 0.0 25 0.0 0.0 0.0 0.11385273737314253 0.0 26 0.0 0.0 0.0 0.12366762852599965 0.0 27 0.0 0.0 0.0 0.13348251967885677 0.0 28 0.0 0.0 0.0 0.1560567693304281 0.0 29 0.0 0.0 0.0 0.1962978230571423 0.0 30 0.0 0.0 0.0 0.2227980291698565 0.0 31 0.0 0.0 0.0 0.2816873760869992 0.0 32 0.0 0.0 0.0 0.32290991892899906 0.0 33 0.0 0.0 0.0 0.3749288420391418 0.0 34 0.0 0.0 0.0 0.43479967807157016 0.0 35 0.0 0.0 0.0 0.5260781657931414 0.0 36 0.0 0.0 0.0 0.7105981194668551 0.0 37 0.0 0.0 0.0 1.0197671907818542 0.0 38 0.0 0.0 0.0 1.4202147498184245 0.0 39 0.0 0.0 0.0 1.9384410026892802 0.0 40 0.0 0.0 0.0 2.5724829711638497 0.0 41 0.0 0.0 0.0 3.4715270007655614 0.0 42 0.0 0.0 0.0 4.479516322163987 0.0 43 0.0 0.0 0.0 5.402116090532556 0.0 44 0.0 0.0 0.0 6.302141609249554 0.0 45 0.0 0.0 0.0 7.264000942229551 0.0 46 0.0 0.0 0.0 8.189545177943977 0.0 47 0.0 0.0 0.0 9.112144946312545 0.0 48 0.0 0.0 0.0 10.0151149323754 0.0 49 0.0 0.0 0.0 10.756139214416113 0.0 50 0.0 0.0 0.0 11.45005201892311 0.0 51 0.0 0.0 0.0 12.221502463537679 0.0 52 0.0 0.0 0.0 13.086194374104391 0.0 53 0.0 0.0 0.0 13.944997349979388 0.0 54 0.0 0.0 0.0 14.921579019688672 0.0 55 0.0 0.0 0.0 15.787252419370668 0.0 56 0.0 0.0 0.0 16.630351569401096 0.0 57 0.0 0.0 0.0 17.314449482755236 0.0 58 0.0 0.0 0.0 18.03682547160552 0.0 59 0.0 0.0 0.0 18.67970084211766 0.0 60 0.0 0.0 0.0 19.274483245980804 0.0 61 0.0 0.0 0.0 19.93895137702923 0.0 62 0.0 0.0 0.0 20.70254990872151 0.0 63 0.0 0.0 0.0 21.452407592799794 0.0 64 0.0 0.0 0.0 22.13356103880808 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGATA 15 0.002219071 70.00001 43 CGCGAGA 15 0.002219071 70.00001 32 CCTCGTG 15 0.002219071 70.00001 7 AATATCG 25 2.822433E-6 70.00001 36 ATCGTAT 25 2.822433E-6 70.00001 39 ATATCGT 25 2.822433E-6 70.00001 37 ATGGTGC 15 0.002219071 70.00001 63 TATACAG 15 0.002219071 70.00001 2 GCTCGCG 15 0.002219071 70.00001 29 GGCTCGC 15 0.002219071 70.00001 28 CGTGGAG 20 0.006933951 52.5 10 AGCCGCT 20 0.006933951 52.5 70 GATCGGC 20 0.006933951 52.5 35 ACGTTGG 20 0.006933951 52.5 16 CCTAGAT 20 0.006933951 52.5 42 GTTTAGT 20 0.006933951 52.5 53 CGTTGGG 20 0.006933951 52.5 17 TCGTGGA 20 0.006933951 52.5 9 CTTTGCG 20 0.006933951 52.5 26 GTCCGTA 20 0.006933951 52.5 11 >>END_MODULE