FastQCFastQC Report
Wed 25 May 2016
SRR1780617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717754
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG300.005694595629.38467419
CGGATGG300.005696540729.38261818
AACGAGA300.00573360129.3436623
ATCGTAC703.5886842E-828.2838325
GCATAAG655.9217564E-727.07891
CGAATTT508.2421E-526.44435718
CGGTCGT756.994378E-826.40376710
CATCGTT1006.184564E-1126.37984332
TCGTTGT1107.2759576E-1226.00915534
CATCGTA801.297667E-724.77081524
TCGTACT801.3153112E-724.73627326
CGTCGCG450.001394795424.4530537
TCTCGAA551.588937E-424.0084523
CGAACTG551.5987408E-423.98668926
CGAGAAT1205.875336E-1022.02926614
CTATCGG703.2042786E-522.00774838
ACATCGT904.063204E-722.00774623
CGTGCGA1205.966285E-1022.0031410
AATGACG500.002586697521.98933229
GACGTGA500.002590889621.98320232
Produced by FastQC (version 0.11.5)