FastQCFastQC Report
Wed 25 May 2016
SRR1780617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717754
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT139131.9384078667621498No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT54150.7544367568832776Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC21560.30038146774521635No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG12120.16886008298107708No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC10980.15297720388879754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA207.8469724E-444.00856814
ACGGTCC207.8469724E-444.00856813
TCGTTGC207.857751E-443.99630722
CCGTCTT6500.037.9071544
GCCGTCT6850.035.97028743
AGCGATC250.00235312735.19704417
TTCGGTA1200.031.16405126
TGCCGTC7950.030.99326742
ATTGGCG2300.029.64968721
GGGCGAC300.00571654929.3615551
GGGTTAC300.00571654929.3615551
AGCGTAC300.00574583429.3308728
TGAAGGT2250.029.33087228
GCCGGTC300.00574583429.33087227
GAAGTCG300.00574583429.33087221
ATGCCGT22800.028.85046644
TCGTTCG1300.028.76681723
CGTTCGG1300.028.76681724
TCGTTGT1100.027.9976534
GCGAAGT554.9606024E-627.9976532
Produced by FastQC (version 0.11.5)