FastQCFastQC Report
Wed 25 May 2016
SRR1780616_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780616_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63495
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCCGT207.874193E-569.99448459
GATAAGT150.00221589569.99448456
TTGCTCG150.00221589569.99448458
CGTTGAT150.00221589569.99448416
GCGTTGA150.00221589569.99448415
CTCGGGG150.00221589569.99448461
AATTGAC308.293455E-658.3287451
GCCCGTG252.374726E-455.99558660
TCTGCGC200.00692411452.495862
AAATCGT200.00692411452.4958632
GGCGTTG200.00692411452.4958614
TGTATAA200.00692411452.4958629
CGGGGGA200.00692411452.4958663
GTATGAC200.00692411452.495863
ACCAGTG407.3758565E-752.4958643
TGGGCGT200.00692411452.4958612
TATGTCG200.00692411452.495864
GAAGGCC200.00692411452.4958627
GTCTAGG305.6822365E-446.9218181
GCCGGTG305.8395433E-446.6629949
Produced by FastQC (version 0.11.5)