##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780616_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63495 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.87484053862509 34.0 31.0 34.0 12.0 34.0 2 30.939081817465944 34.0 32.0 34.0 23.0 34.0 3 31.199354279864558 34.0 33.0 34.0 23.0 34.0 4 31.6475155524057 34.0 34.0 34.0 23.0 34.0 5 31.542294668871566 34.0 34.0 34.0 23.0 34.0 6 33.97878573115993 38.0 34.0 38.0 23.0 38.0 7 33.42839593668793 37.0 34.0 38.0 21.0 38.0 8 32.28766044570439 37.0 31.0 38.0 11.0 38.0 9 33.163934168044726 37.0 34.0 38.0 21.0 38.0 10-11 33.58569966139066 37.0 34.0 38.0 21.5 38.0 12-13 33.969958264430275 37.0 34.0 38.0 22.5 38.0 14-15 33.58274667296638 37.0 34.0 38.0 21.0 38.0 16-17 33.49444838176234 37.0 34.0 38.0 21.0 38.0 18-19 33.455492558469174 37.0 34.0 38.0 21.0 38.0 20-21 33.52071816678479 37.0 34.0 38.0 21.0 38.0 22-23 33.52431687534452 37.0 34.0 38.0 21.0 38.0 24-25 34.00197653358532 37.5 34.0 38.0 22.5 38.0 26-27 34.055508307740766 38.0 34.0 38.0 22.5 38.0 28-29 34.20855972911253 38.0 34.5 38.0 23.0 38.0 30-31 34.060910307898254 38.0 34.0 38.0 23.0 38.0 32-33 33.8123868021104 37.5 34.0 38.0 21.5 38.0 34-35 33.678037640759115 37.0 34.0 38.0 21.0 38.0 36-37 33.63228600677219 37.0 34.0 38.0 21.0 38.0 38-39 34.154043625482316 38.0 34.5 38.0 23.0 38.0 40-41 33.63897944720057 37.5 34.0 38.0 21.0 38.0 42-43 33.98155760296086 37.5 34.0 38.0 22.5 38.0 44-45 33.05087802189149 37.0 33.5 38.0 16.0 38.0 46-47 33.732703362469486 37.0 34.0 38.0 21.5 38.0 48-49 33.46524135758721 37.0 34.0 38.0 21.0 38.0 50-51 33.42766359555871 37.0 34.0 38.0 21.0 38.0 52-53 33.43915268918812 37.0 34.0 38.0 21.0 38.0 54-55 33.143027010000786 37.0 33.5 38.0 21.0 38.0 56-57 33.25240570123631 37.0 33.5 38.0 21.0 38.0 58-59 33.14702732498622 37.0 33.0 38.0 21.0 38.0 60-61 33.15741396960391 37.0 32.5 38.0 20.5 38.0 62-63 33.20292148988109 37.0 33.0 38.0 20.5 38.0 64-65 33.238530592960075 37.0 33.0 38.0 21.0 38.0 66-67 33.161461532404125 37.0 32.5 38.0 20.5 38.0 68-69 32.74831876525711 37.0 31.5 38.0 15.5 38.0 70-71 32.67579336955666 37.0 31.5 38.0 19.0 38.0 72-73 32.79428301441058 37.0 32.0 38.0 19.0 38.0 74-75 32.439483423891645 37.0 31.0 38.0 15.0 38.0 76 31.650019686589495 36.0 29.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.955324393793557 1101 2 -1.4415035508150815 1101 3 0.1498881220950068 1101 4 -2.181825133910266 1101 5 -0.546981981972209 1101 6 -1.571987078454974 1101 7 -1.0610724396211495 1101 8 -1.0576082137194405 1101 9 -0.7006164374787716 1101 10-11 -1.1193300803980506 1101 12-13 -0.8011843019710341 1101 14-15 -0.4474602560776404 1101 16-17 -1.6254989541283535 1101 18-19 0.2158551364588135 1101 20-21 -0.8305652544600903 1101 22-23 0.19940117675381686 1101 24-25 -1.6830351041109708 1101 26-27 -1.859543768054813 1101 28-29 0.0943899370487955 1101 30-31 -1.7543400677124197 1101 32-33 -0.3674063879923253 1101 34-35 -0.923125980913376 1101 36-37 0.5562471919084615 1101 38-39 -0.14764990019195778 1101 40-41 0.5547528165221323 1101 42-43 -1.0416791697537775 1101 44-45 0.09895393217286141 1101 46-47 0.5220280079312545 1101 48-49 -1.241762304091722 1101 50-51 -1.6356324816863044 1101 52-53 -0.371120607598975 1101 54-55 -1.934450531112006 1101 56-57 -0.05340579759837283 1101 58-59 -0.6044076802478884 1101 60-61 0.3417140333820825 1101 62-63 -1.5431755899511828 1101 64-65 -0.19835121790235632 1101 66-67 -1.2137630307326646 1101 68-69 0.5424306454390546 1101 70-71 0.3760473519157088 1101 72-73 -0.24688375372098648 1101 74-75 -1.411358285142832 1101 76 -0.7578813154714936 1102 1 -0.081982870452034 1102 2 0.19084698153545077 1102 3 0.14976527197215717 1102 4 -0.08935994144507475 1102 5 0.4317649467747202 1102 6 0.9584388019709067 1102 7 0.8889275603788533 1102 8 1.4992713931601642 1102 9 0.6482779114155761 1102 10-11 -0.48280264387062033 1102 12-13 -5.074075374862105 1102 14-15 -1.321231754849137 1102 16-17 -3.2927389213683256 1102 18-19 -0.4790261084224312 1102 20-21 -0.22749400138884113 1102 22-23 -1.0054309280782832 1102 24-25 0.2448109237350593 1102 26-27 -5.194822228333273 1102 28-29 0.30882482648367926 1102 30-31 0.5842348708625167 1102 32-33 -7.352193447779385 1102 34-35 -0.6400792978666843 1102 36-37 0.9945764302377071 1102 38-39 0.8223951448530897 1102 40-41 0.7388027755720898 1102 42-43 0.7592831562085465 1102 44-45 -5.294350736131804 1102 46-47 0.1788871397903904 1102 48-49 0.2844047720753551 1102 50-51 -6.750210696264517 1102 52-53 -1.3718167582951324 1102 54-55 1.0430883714268973 1102 56-57 -0.943823488016065 1102 58-59 1.4249125490723458 1102 60-61 1.4437615354295872 1102 62-63 -4.634739881515479 1102 64-65 -1.1899564595075987 1102 66-67 0.547252530282897 1102 68-69 -8.260763457755047 1102 70-71 -0.40284290197454453 1102 72-73 0.0495945427573119 1102 74-75 -6.284781708566257 1102 76 -1.0311818887720676 1103 1 0.15969230830179626 1103 2 -0.5974403865271967 1103 3 0.4077836580804295 1103 4 0.24438197207211942 1103 5 0.692541249124055 1103 6 -0.5939275582606243 1103 7 0.5091522794799772 1103 8 0.9075451418833538 1103 9 0.4153872066147599 1103 10-11 0.9984196918461024 1103 12-13 -1.3992711528668735 1103 14-15 -0.5952358406509717 1103 16-17 -0.27779169630873923 1103 18-19 -0.8200543685518085 1103 20-21 -0.5082907327473691 1103 22-23 -1.0621384527633353 1103 24-25 0.4299317953727808 1103 26-27 -0.9063475943530221 1103 28-29 0.9714397396603971 1103 30-31 0.33668635196568175 1103 32-33 -4.2575901110412175 1103 34-35 -1.1397217902058117 1103 36-37 -0.05322745127404005 1103 38-39 0.569211624253839 1103 40-41 1.0625344745846874 1103 42-43 0.5205361354390519 1103 44-45 -5.020959520605757 1103 46-47 0.2895272487451024 1103 48-49 0.18884808157483945 1103 50-51 -0.045137492314914596 1103 52-53 -0.613832420535509 1103 54-55 0.5086484667622742 1103 56-57 1.2773716090217277 1103 58-59 1.0121273941523583 1103 60-61 0.986338995422777 1103 62-63 1.0195331862407429 1103 64-65 1.0645549466891993 1103 66-67 0.6690945459451356 1103 68-69 -3.2129595762433 1103 70-71 0.39620374426311145 1103 72-73 1.4729006374117262 1103 74-75 -0.9939577385288061 1103 76 1.3145450434717212 1104 1 -1.575217542756473 1104 2 0.6142789758976797 1104 3 0.44247140305038 1104 4 -0.008035064771359401 1104 5 0.2676000419121429 1104 6 0.8272901057059272 1104 7 1.619160118518387 1104 8 0.8658675876633382 1104 9 0.8163540213521543 1104 10-11 -0.0811465480284781 1104 12-13 0.047260072636134964 1104 14-15 -0.3035256237709092 1104 16-17 -0.3114668988172298 1104 18-19 1.3204277323104776 1104 20-21 1.2705503960741638 1104 22-23 0.5700799951070579 1104 24-25 0.8517700498802299 1104 26-27 1.039497715288995 1104 28-29 0.1315520992109569 1104 30-31 -0.40165307839752273 1104 32-33 -0.17648872614443434 1104 34-35 0.3923202793003284 1104 36-37 0.44177516173876086 1104 38-39 0.06793778051199695 1104 40-41 0.9361953055227659 1104 42-43 1.0714572224523806 1104 44-45 -2.9136953450112983 1104 46-47 0.32953900730271357 1104 48-49 0.8188128059019917 1104 50-51 1.3962656955607073 1104 52-53 0.18607612401635976 1104 54-55 -0.551908104964923 1104 56-57 0.6396331716033856 1104 58-59 1.4542818232090582 1104 60-61 0.7666826066064729 1104 62-63 0.6325786526635255 1104 64-65 -0.11499169558934597 1104 66-67 0.20727719554012225 1104 68-69 -3.969590096232846 1104 70-71 -0.043892937210621596 1104 72-73 0.786821463123772 1104 74-75 -1.0867196930623777 1104 76 0.8090577165838191 1105 1 -0.9999189883881527 1105 2 -0.346908956220485 1105 3 -0.9730848508779673 1105 4 -0.21155486363999643 1105 5 -0.0915765765668013 1105 6 -0.3219870784549741 1105 7 0.5754140468653404 1105 8 0.12482421871298754 1105 9 -0.20872454558688247 1105 10-11 0.1266158655478975 1105 12-13 -1.2903734911602243 1105 14-15 -0.07110890472628029 1105 16-17 1.0609875323581335 1105 18-19 -0.061171890568203935 1105 20-21 -0.42380849770333384 1105 22-23 0.015617392970035837 1105 24-25 0.7034513823755191 1105 26-27 0.5600508265397792 1105 28-29 -1.3508803332214754 1105 30-31 -0.11650222987458392 1105 32-33 0.4021882066022684 1105 34-35 0.40930645151906475 1105 36-37 0.21840935407063 1105 38-39 0.48545820791615313 1105 40-41 0.09596903273834556 1105 42-43 0.1657532626786491 1105 44-45 0.876656634875566 1105 46-47 0.3956766565799015 1105 48-49 0.2325620202325993 1105 50-51 0.023826977773154567 1105 52-53 -1.2346341211124923 1105 54-55 -0.9189099905714642 1105 56-57 -0.8101625543551307 1105 58-59 -0.2719752478154476 1105 60-61 -0.1772048855368311 1105 62-63 0.3433108965353071 1105 64-65 0.154351484800344 1105 66-67 0.38083156386193195 1105 68-69 1.3802684832768932 1105 70-71 1.237533838402193 1105 72-73 0.7531162462790135 1105 74-75 -0.7640609878455393 1105 76 -0.9497732073633856 1106 1 -0.1550575664566658 1106 2 0.6888975509235991 1106 3 -0.28405059369084285 1106 4 0.27014594544934667 1106 5 0.44531445459174535 1106 6 -0.2176661970640339 1106 7 1.2940231017801267 1106 8 0.5025140104155241 1106 9 1.3222790694931703 1106 10-11 0.7267707975499604 1106 12-13 0.4195938009726774 1106 14-15 0.2016402775770345 1106 16-17 0.9258868265567841 1106 18-19 0.02274961055074698 1106 20-21 0.04338034719251738 1106 22-23 0.16936158301995619 1106 24-25 0.9439230642484802 1106 26-27 1.2442821916628688 1106 28-29 0.01849477437018976 1106 30-31 -0.007137451083053747 1106 32-33 1.228543861620345 1106 34-35 0.7825032151616895 1106 36-37 0.4196488100871534 1106 38-39 0.5213335737278229 1106 40-41 0.25644415754468497 1106 42-43 0.7747048894646724 1106 44-45 1.7103973819921094 1106 46-47 1.3442220172271675 1106 48-49 1.015209636001238 1106 50-51 1.5199881070553047 1106 52-53 0.497918813988214 1106 54-55 0.137915624852873 1106 56-57 0.9626038426150885 1106 58-59 0.14064310301945682 1106 60-61 1.3797584472226774 1106 62-63 1.1552234460274704 1106 64-65 1.008267821519226 1106 66-67 1.6817439412074293 1106 68-69 1.9160749904209808 1106 70-71 1.0972343031982064 1106 72-73 1.2371066060655522 1106 74-75 -1.77480293998876 1106 76 -0.6173235602640581 1107 1 -0.8544137004915591 1107 2 -0.9203519644461018 1107 3 -0.9181201035219146 1107 4 -0.38922818913940915 1107 5 -0.2471876223952414 1107 6 -0.09093537457718526 1107 7 -0.34368113527332156 1107 8 -0.08066931830228441 1107 9 -0.6605165549875878 1107 10-11 -0.9924205621841864 1107 12-13 -0.142767733228375 1107 14-15 -0.32172582599537947 1107 16-17 -0.656638789616018 1107 18-19 0.09210366759864996 1107 20-21 0.08974908161511763 1107 22-23 -1.033560045337829 1107 24-25 -0.6980909207972203 1107 26-27 -1.3584421229314287 1107 28-29 -2.235295726876 1107 30-31 -1.4568224413904431 1107 32-33 -0.30622836214038784 1107 34-35 -0.2919567682224198 1107 36-37 -1.3692081547425303 1107 38-39 -0.6768802174657509 1107 40-41 0.061068915229533616 1107 42-43 -0.9917379241603612 1107 44-45 -0.30872820648753674 1107 46-47 -0.4353750472978888 1107 48-49 -0.6282928563831476 1107 50-51 0.06670300597527046 1107 52-53 0.07320959216600897 1107 54-55 0.38590787076813626 1107 56-57 0.23764884399974306 1107 58-59 -0.5044517460528226 1107 60-61 -0.6996343802489307 1107 62-63 -0.477444097589256 1107 64-65 -0.436086235528677 1107 66-67 0.38883685175851923 1107 68-69 0.706281996790409 1107 70-71 -0.580988488272304 1107 72-73 -0.04337317792780482 1107 74-75 0.17699365375258225 1107 76 -1.1368472379156778 1108 1 -0.23266207630365443 1108 2 -0.2586827990288114 1108 3 0.03983841721771597 1108 4 0.15849794779212445 1108 5 0.3167179248311456 1108 6 -0.7607879482903286 1108 7 0.510766640838618 1108 8 0.970117788144492 1108 9 -0.7916384940180734 1108 10-11 0.05230707399771717 1108 12-13 -0.07339616901048629 1108 14-15 0.7435540340745845 1108 16-17 -1.0250795692262145 1108 18-19 0.34532080902793894 1108 20-21 0.26837851689747794 1108 22-23 -0.43860752159970673 1108 24-25 -1.2317303510820778 1108 26-27 -0.4516142868494697 1108 28-29 0.09994290380866033 1108 30-31 0.2121868039868673 1108 32-33 0.27669892421643283 1108 34-35 0.5336929069399972 1108 36-37 0.31416890672673503 1108 38-39 0.42469399542780195 1108 40-41 0.7101193899921512 1108 42-43 0.6872350893328871 1108 44-45 0.016203077870287075 1108 46-47 0.053443043656635325 1108 48-49 0.2045719612080532 1108 50-51 0.8501550299632754 1108 52-53 0.3376398770235767 1108 54-55 0.19862651144779875 1108 56-57 0.6396665851291843 1108 58-59 -0.7175639399558662 1108 60-61 1.0331554748235234 1108 62-63 0.39958613729330494 1108 64-65 0.9816736345362926 1108 66-67 0.16935284376251758 1108 68-69 1.8909084335720223 1108 70-71 2.032423555705712 1108 72-73 0.657745012976747 1108 74-75 1.2083217397096035 1108 76 1.4775955406950168 1109 1 0.21593485428133263 1109 2 0.26716321651090524 1109 3 0.8910552668934351 1109 4 0.5753090952910043 1109 5 0.864878202234344 1109 6 0.8628234303778797 1109 7 -0.2887260709060655 1109 8 0.7847970406969864 1109 9 1.4349716645897743 1109 10-11 1.3393744341723846 1109 12-13 0.19821174211774917 1109 14-15 0.617823603200641 1109 16-17 -0.02705414059974487 1109 18-19 1.1216002670642808 1109 20-21 1.0536639468082214 1109 22-23 1.891081684076788 1109 24-25 1.5928217583380757 1109 26-27 1.3113568811977956 1109 28-29 0.501663831054536 1109 30-31 0.8180591471448935 1109 32-33 1.312289369217396 1109 34-35 0.40542603478947825 1109 36-37 0.7646119812788399 1109 38-39 1.182544120644522 1109 40-41 0.8248532241923741 1109 42-43 1.297762925973231 1109 44-45 1.291936719429394 1109 46-47 -0.14891038856580252 1109 48-49 0.3644961890382774 1109 50-51 1.6597774533884362 1109 52-53 1.6388824121805854 1109 54-55 0.44711748942249585 1109 56-57 0.9707524388503614 1109 58-59 1.1070523831674848 1109 60-61 1.7012187895348845 1109 62-63 0.5046879160128768 1109 64-65 0.7138864387487089 1109 66-67 0.8883055182632589 1109 68-69 2.1284188682987875 1109 70-71 0.13893803589578368 1109 72-73 -0.22076266056078708 1109 74-75 1.953393337988679 1109 76 0.08637355392303903 1110 1 0.5219057265001474 1110 2 0.12614212954736104 1110 3 1.0683792065790954E-4 1110 4 0.24334280859871527 1110 5 -0.04295917303610608 1110 6 -0.4771190890783146 1110 7 0.6369044389915643 1110 8 2.739103237616284 1110 9 -0.8420005962039738 1110 10-11 -0.2770398132522942 1110 12-13 0.27849074880228386 1110 14-15 -0.11944510219542792 1110 16-17 0.5637683478557065 1110 18-19 0.4239866784053845 1110 20-21 0.752117138915942 1110 22-23 0.8821851167631962 1110 24-25 0.3473507730957124 1110 26-27 -0.7898476268268837 1110 28-29 0.41334400672540283 1110 30-31 -0.03515556529092123 1110 32-33 1.6496807858867513 1110 34-35 0.9504094041827074 1110 36-37 0.2330945595072791 1110 38-39 0.6274047787644577 1110 40-41 0.48290857343511817 1110 42-43 0.43892932891830583 1110 44-45 1.7389125323915806 1110 46-47 0.4392479735616206 1110 48-49 0.8177205892061963 1110 50-51 0.24446437817934452 1110 52-53 0.28397234662692483 1110 54-55 0.0870890720749955 1110 56-57 0.2393609575961264 1110 58-59 -3.230403618382553 1110 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54-55 -0.9403077196614973 2116 56-57 -0.568998313190896 2116 58-59 -1.0643469094178855 2116 60-61 -2.14485241472282 2116 62-63 0.2557209397145783 2116 64-65 0.6292115519681047 2116 66-67 -1.1365201073358975 2116 68-69 -1.103259423327934 2116 70-71 -4.092227875974899 2116 72-73 -0.6306995221521348 2116 74-75 -1.7346830892368672 2116 76 -3.0370273253867346 2117 1 0.4134434423655833 2117 2 0.09207039580234522 2117 3 0.814022753620943 2117 4 0.34990224564320016 2117 5 -0.2976534609045558 2117 6 -0.66779872861445 2117 7 -1.9632463526646262 2117 8 -1.6606382624016653 2117 9 0.9582722648881941 2117 10-11 -0.5327932329916436 2117 12-13 0.9880552481380818 2117 14-15 -0.33647737879041273 2117 16-17 0.2905661793280814 2117 18-19 0.3075539781576566 2117 20-21 0.02569628658405776 2117 22-23 -0.028901660244663674 2117 24-25 0.6089742965940843 2117 26-27 1.14039328079528 2117 28-29 0.6331048942420168 2117 30-31 0.6421682418393715 2117 32-33 1.5645014515242082 2117 34-35 0.14360223798875893 2117 36-37 -1.2966149870406696 2117 38-39 0.5047968011677852 2117 40-41 -0.9824093456400291 2117 42-43 -0.8123900980734078 2117 44-45 0.7700761413385493 2117 46-47 0.023475884379131173 2117 48-49 -0.8546127321595449 2117 50-51 0.7278054904473166 2117 52-53 1.2432406480666245 2117 54-55 -0.8159554832691285 2117 56-57 -0.840379106310813 2117 58-59 -0.5615169634441273 2117 60-61 0.9249774209933008 2117 62-63 -0.5383913025582032 2117 64-65 -0.42871045913116745 2117 66-67 -1.7877935830240865 2117 68-69 -0.11553477339592888 2117 70-71 1.00713262974012 2117 72-73 1.2077914183454936 2117 74-75 -0.09513367543996054 2117 76 -0.5157292254933168 2118 1 0.34271497505505977 2118 2 0.9064889503566107 2118 3 0.23710105757383815 2118 4 0.7866954097547847 2118 5 0.009063948351180073 2118 6 1.5113202172800655 2118 7 1.3363264043094887 2118 8 -3.0932667045802447 2118 9 -0.9287057985161127 2118 10-11 -0.05160628724073746 2118 12-13 1.2727104019814774 2118 14-15 -0.5992138883226019 2118 16-17 0.1620342438094653 2118 18-19 -0.20607112506282022 2118 20-21 -3.1756285258239387 2118 22-23 0.1683122843932523 2118 24-25 0.14214846095698874 2118 26-27 1.288187055254042 2118 28-29 0.39022261944217007 2118 30-31 -0.10162955373023408 2118 32-33 0.27684841594456344 2118 34-35 1.2452070920439766 2118 36-37 -0.07037364858520334 2118 38-39 -0.00265302470375417 2118 40-41 -1.2540856462180656 2118 42-43 -0.2402614225267854 2118 44-45 0.10885472892337589 2118 46-47 0.3698838117119152 2118 48-49 -0.6418169830481375 2118 50-51 -0.6514893790460903 2118 52-53 -1.3836577111765394 2118 54-55 0.19691566167036711 2118 56-57 1.7104592234920801 2118 58-59 1.451677306472945 2118 60-61 -1.8998888411687638 2118 62-63 0.09633386169699776 2118 64-65 0.48275329701718306 2118 66-67 -1.2019679987064222 2118 68-69 1.9140132106632421 2118 70-71 0.33697142627909216 2118 72-73 -1.2043903933085467 2118 74-75 0.5038502760194845 2118 76 -0.0021400250481207195 2119 1 -0.0036611921303553174 2119 2 0.038399429087899506 2119 3 0.4967349689418512 2119 4 0.35213190004676775 2119 5 0.053676368686144116 2119 6 -0.08550059196848991 2119 7 -2.2213288498775583 2119 8 0.5446093884981593 2119 9 -0.1366871235494571 2119 10-11 -0.3360660471340182 2119 12-13 -0.24790987369659945 2119 14-15 -0.6402876989050768 2119 16-17 -0.5367948654242696 2119 18-19 -0.5940027983991172 2119 20-21 -0.6972492461440822 2119 22-23 0.5001634775161179 2119 24-25 -0.09545714153300366 2119 26-27 -0.14237467588571917 2119 28-29 0.2661674838263437 2119 30-31 0.002416689044338227 2119 32-33 -0.008778529364469989 2119 34-35 0.015456832669443088 2119 36-37 -1.1311228704615957 2119 38-39 -0.27568177822383433 2119 40-41 0.45348809025740167 2119 42-43 -0.5642016922762991 2119 44-45 -1.4965069132879805 2119 46-47 -0.9985021375988907 2119 48-49 -1.0175765799059988 2119 50-51 0.2916890706352504 2119 52-53 -1.9676209540993241 2119 54-55 -1.3194990411605154 2119 56-57 0.9277965586039798 2119 58-59 0.9075465817063773 2119 60-61 0.16777085943891024 2119 62-63 -0.26597531275090347 2119 64-65 -0.28523617978731863 2119 66-67 0.7700380780684455 2119 68-69 -0.29532056731215306 2119 70-71 -0.9556401047717458 2119 72-73 -2.250522007359237 2119 74-75 0.04342341963887719 2119 76 1.0295407219505428 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 17.0 13 67.0 14 229.0 15 459.0 16 554.0 17 642.0 18 762.0 19 709.0 20 645.0 21 588.0 22 566.0 23 601.0 24 612.0 25 684.0 26 921.0 27 1027.0 28 1184.0 29 1522.0 30 1865.0 31 2300.0 32 2953.0 33 3999.0 34 5235.0 35 7883.0 36 12915.0 37 14556.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.86919296020295 25.64927857935627 11.44918344696369 28.03234501347709 2 16.651704858650287 20.401606425702813 36.56035908339239 26.38632963225451 3 17.63918418773132 25.822505709110956 29.189699976376094 27.348610126781637 4 12.339554295613828 18.930624458618787 40.11811953697141 28.611701708795966 5 12.993149066855658 37.23285298054965 35.55555555555556 14.218442397039139 6 25.84140483502638 37.62028506181589 18.048665249232222 18.489644853925505 7 24.795653201039453 30.101582801795416 25.00984329474762 20.092920702417512 8 21.74974407433656 34.75549255846917 19.190487439955902 24.304275927238365 9 23.3640444129459 15.672100165367354 22.466335931963147 38.4975194897236 10-11 24.027088747145445 25.35553980628396 27.392708087250966 23.22466335931963 12-13 22.897078510118906 23.585321678872354 26.478462871092212 27.03913693991653 14-15 21.274903535711474 25.198834553901882 23.9318056539885 29.594456256398143 16-17 22.052917552563194 27.402945113788483 25.75793369556658 24.786203638081737 18-19 21.700921332388376 26.73202614379085 26.682415938262853 24.884636585557917 20-21 20.867784864949996 27.382471060713442 26.379242460036224 25.370501614300338 22-23 22.65768958185684 26.656429640129144 26.64934246791086 24.036538310103158 24-25 22.055279943302622 27.641546578470745 26.13197889597606 24.17119458225057 26-27 20.61343412867155 28.481770218127412 26.73123868021104 24.17355697299 28-29 21.717458067564372 26.602882116702105 28.795180722891565 22.884479092841957 30-31 21.716670603984568 26.94070399244035 27.780927632097015 23.56169777147807 32-33 20.50318922749823 28.764469643279 27.140719741711944 23.591621387510827 34-35 21.72533270336247 27.929758248680997 27.02417513190015 23.320733916056383 36-37 22.244271202456886 27.07772265532719 26.605244507441533 24.07276163477439 38-39 21.61193794786991 27.531301677297428 27.702968737695883 23.153791637136784 40-41 21.465469722025357 26.4690133081345 28.006142215922512 24.05937475391763 42-43 20.9599181037877 26.53673517599811 28.14079848806993 24.362548232144263 44-45 20.288211670210252 26.154027876210723 28.74084573588472 24.816914717694306 46-47 21.026065044491695 26.25009843294748 28.744783053783763 23.97905346877707 48-49 19.661390660681942 26.540672493897155 29.449562957713205 24.348373887707695 50-51 19.946452476572958 26.84069611780455 28.535317741554454 24.677533664068037 52-53 19.3574297188755 26.807622647452554 28.729821245767383 25.10512638790456 54-55 19.09441688321915 27.35805968973935 28.291991495393336 25.25553193164816 56-57 19.470037010788253 26.706827309236946 28.424285376801322 25.398850303173475 58-59 19.71966296558784 26.683203401842665 28.00141743444366 25.595716198125835 60-61 19.428301441058352 26.20600047247815 28.476257973068748 25.889440113394755 62-63 20.128356563508937 26.262697850224427 27.78958973147492 25.819355854791716 64-65 19.856681628474686 26.79029844869675 26.79187337585637 26.561146546972203 66-67 20.27718718009292 26.63280573273486 26.683203401842665 26.406803685329557 68-69 19.980313410504763 27.147019450350424 26.3942042680526 26.478462871092212 70-71 20.502401763918417 26.9950389794472 25.880778014016855 26.62178124261753 72-73 20.368532955350815 27.262776596582405 25.613040396881644 26.755650051185132 74-75 20.171667060398455 27.85652413575872 25.405937475391767 26.565871328451056 76 20.7512402551382 27.49980313410505 25.564217654933458 26.184738955823295 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.5 12 1.0 13 1.0 14 2.0 15 3.5 16 6.0 17 8.0 18 9.0 19 12.0 20 15.0 21 16.0 22 18.5 23 29.0 24 41.0 25 45.0 26 77.5 27 177.0 28 276.0 29 308.0 30 386.5 31 524.5 32 714.5 33 845.0 34 997.5 35 1344.5 36 1718.0 37 1897.0 38 2184.5 39 2779.5 40 3376.0 41 3901.0 42 4137.0 43 4183.5 44 4521.0 45 4830.5 46 4849.0 47 4889.5 48 4705.5 49 4229.5 50 3978.0 51 3719.5 52 3152.0 53 2542.0 54 2241.0 55 2068.5 56 1662.5 57 1201.5 58 974.0 59 835.5 60 618.0 61 466.5 62 394.0 63 347.0 64 257.0 65 188.5 66 145.0 67 127.0 68 93.5 69 54.0 70 45.0 71 42.0 72 33.0 73 20.0 74 15.5 75 15.0 76 12.5 77 9.0 78 11.0 79 14.0 80 8.5 81 3.5 82 5.0 83 6.0 84 4.0 85 1.5 86 0.5 87 0.0 88 0.5 89 2.0 90 2.0 91 1.0 92 1.0 93 2.0 94 2.5 95 1.0 96 0.0 97 0.5 98 1.5 99 4.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6693440428380187 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 63495.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.44853925505945 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12973606383572 42.40963855421687 2 9.924111377713297 11.204031813528625 3 5.259193125383628 8.906213087644696 4 3.0383349143462977 6.860382707299788 5 1.8860554656548183 5.323253799511773 6 1.252720272306233 4.242853768013229 7 0.853747000725406 3.3734939759036147 8 0.6082249874448971 2.7466729663753053 9 0.468723843535517 2.381289865343728 >10 1.579152949054182 12.552169462162373 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0031498543192377353 0.0 2 0.0 0.0 0.0 0.009449562957713206 0.0 3 0.0 0.0 0.0 0.014174344436569808 0.0 4 0.0 0.0 0.0 0.02204898023466415 0.0 5 0.0 0.0 0.0 0.02204898023466415 0.0 6 0.0 0.0 0.0 0.02204898023466415 0.0 7 0.0 0.0 0.0 0.029923616032758484 0.0 8 0.0 0.0 0.0 0.031498543192377354 0.0 9 0.0 0.0 0.0 0.06772186786361131 0.0 10 0.0 0.0 0.0 0.09292070241751318 0.0 11 0.0 0.0 0.0 0.09764548389636979 0.0 12 0.0 0.0 0.0 0.09922041105598867 0.0 13 0.0 0.0 0.0 0.10079533821560753 0.0 14 0.0 0.0 0.0 0.10394519253484526 0.0 15 0.0 0.0 0.0 0.10394519253484526 0.0 16 0.0 0.0 0.0 0.10552011969446413 0.0 17 0.0 0.0 0.0 0.11811953697141507 0.0 18 0.0 0.0 0.0 0.12756909992912827 0.0 19 0.0 0.0 0.0 0.14016851720607923 0.0 20 0.0 0.0 0.0 0.14646822584455468 0.0 21 0.0 0.0 0.0 0.1559177888022679 0.0 22 0.0 0.0 0.0 0.1700921332388377 0.0 23 0.0 0.0 0.0 0.1763918418773132 0.0 24 0.0 0.0 0.0 0.18426647767540752 0.0 25 0.0 0.0 0.0 0.18741633199464525 0.0 26 0.0 0.0 0.0 0.20316560359083394 0.0 27 0.0 0.0 0.0 0.24411370974092447 0.0 28 0.0 0.0 0.0 0.2693125442948264 0.0 29 0.0 0.0 0.0 0.30868572328529803 0.0 30 0.0 0.0 0.0 0.33388455783919996 0.0 31 0.0 0.0 0.0 0.3732577368296716 0.0 32 0.0 0.0 0.0 0.41105598866052445 0.0 33 0.0 0.0 0.0 0.45515394912985274 0.0 34 0.0 0.0 0.0 0.5087014725568942 0.0 35 0.0 0.0 0.0 0.6110717379321207 0.0 36 0.0 0.0 0.0 0.7433656193401055 0.0 37 0.0 0.0 0.0 1.1496968265217733 0.0 38 0.0 0.0 0.0 1.5875265768958187 0.0 39 0.0 0.0 0.0 2.0647295062603352 0.0 40 0.0 0.0 0.0 2.6726513898732183 0.0 41 0.0 0.0 0.0 3.3876683203401843 0.0 42 0.0 0.0 0.0 4.096385542168675 0.0 43 0.0 0.0 0.0 4.88227419481849 0.0 44 0.0 0.0 0.0 5.639814158595165 0.0 45 0.0 0.0 0.0 6.389479486573746 0.0 46 0.0 0.0 0.0 7.014725568942437 0.0 47 0.0 0.0 0.0 7.73289235372864 0.0 48 0.0 0.0 0.0 8.47153319158989 0.0 49 0.0 0.0 0.0 9.148751870226002 0.0 50 0.0 0.0 0.0 9.832270257500591 0.0 51 0.0 0.0 0.0 10.58351051263879 0.0 52 0.0 0.0 0.0 11.339475549255846 0.0 53 0.0 0.0 0.0 12.024568863690055 0.0 54 0.0 0.0 0.0 12.692337979368455 0.0 55 0.0 0.0 0.0 13.264036538310103 0.0 56 0.0 0.0 0.0 13.845184660209465 0.0 57 0.0 0.0 0.0 14.396409166076069 0.0 58 0.0 0.0 0.0 15.004331049688952 0.0 59 0.0 0.0 0.0 15.587054098747933 0.0 60 0.0 0.0 0.0 16.21545003543586 0.0 61 0.0 0.0 0.0 16.727301362311994 0.0 62 0.0 0.0 0.0 17.210804000314987 0.0 63 0.0 0.0 0.0 17.724230254350736 0.0 64 0.0 0.0 0.0 18.21718245531144 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCCGT 20 7.874193E-5 69.994484 59 GATAAGT 15 0.002215895 69.994484 56 TTGCTCG 15 0.002215895 69.994484 58 CGTTGAT 15 0.002215895 69.994484 16 GCGTTGA 15 0.002215895 69.994484 15 CTCGGGG 15 0.002215895 69.994484 61 AATTGAC 30 8.293455E-6 58.32874 51 GCCCGTG 25 2.374726E-4 55.995586 60 TCTGCGC 20 0.006924114 52.49586 2 AAATCGT 20 0.006924114 52.49586 32 GGCGTTG 20 0.006924114 52.49586 14 TGTATAA 20 0.006924114 52.49586 29 CGGGGGA 20 0.006924114 52.49586 63 GTATGAC 20 0.006924114 52.49586 3 ACCAGTG 40 7.3758565E-7 52.49586 43 TGGGCGT 20 0.006924114 52.49586 12 TATGTCG 20 0.006924114 52.49586 4 GAAGGCC 20 0.006924114 52.49586 27 GTCTAGG 30 5.6822365E-4 46.921818 1 GCCGGTG 30 5.8395433E-4 46.66299 49 >>END_MODULE