FastQCFastQC Report
Wed 25 May 2016
SRR1780616_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780616_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63495
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT10371.6331994645247656No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4460.7024175131900149TruSeq Adapter, Index 2 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC1720.27088747145444525No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1110.1748169147176943No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG840.13229388140798487No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAG200.006921955452.515
TTGGCGT200.006921955452.523
ACATTAT200.006921955452.512
AGCTATG200.006921955452.512
CACTTCC200.006921955452.517
AGGACGG350.00124682540.010
GATCAGA350.00124682540.016
GACGAAG350.00124682540.021
CGAAGAG350.00124682540.023
ACGAAGA350.00124682540.022
GGGCGTT400.002402305435.030
TTAAAGC400.002402305435.045
TAGGACG400.002402305435.09
CGTTTGG400.002402305435.033
TCTCGTA2850.033.15789438
ATCTCGT2950.032.03389737
CTTCACC552.9619178E-431.818189
CGTATGC3250.031.23076841
CGGATCA450.004278145731.11111314
ACTACGT450.004278145731.1111133