Basic Statistics
Measure | Value |
---|---|
Filename | SRR1780616_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 63495 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT | 1037 | 1.6331994645247656 | No Hit |
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT | 446 | 0.7024175131900149 | TruSeq Adapter, Index 2 (95% over 21bp) |
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC | 172 | 0.27088747145444525 | No Hit |
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC | 111 | 0.1748169147176943 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG | 84 | 0.13229388140798487 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATGAG | 20 | 0.0069219554 | 52.5 | 15 |
TTGGCGT | 20 | 0.0069219554 | 52.5 | 23 |
ACATTAT | 20 | 0.0069219554 | 52.5 | 12 |
AGCTATG | 20 | 0.0069219554 | 52.5 | 12 |
CACTTCC | 20 | 0.0069219554 | 52.5 | 17 |
AGGACGG | 35 | 0.001246825 | 40.0 | 10 |
GATCAGA | 35 | 0.001246825 | 40.0 | 16 |
GACGAAG | 35 | 0.001246825 | 40.0 | 21 |
CGAAGAG | 35 | 0.001246825 | 40.0 | 23 |
ACGAAGA | 35 | 0.001246825 | 40.0 | 22 |
GGGCGTT | 40 | 0.0024023054 | 35.0 | 30 |
TTAAAGC | 40 | 0.0024023054 | 35.0 | 45 |
TAGGACG | 40 | 0.0024023054 | 35.0 | 9 |
CGTTTGG | 40 | 0.0024023054 | 35.0 | 33 |
TCTCGTA | 285 | 0.0 | 33.157894 | 38 |
ATCTCGT | 295 | 0.0 | 32.033897 | 37 |
CTTCACC | 55 | 2.9619178E-4 | 31.81818 | 9 |
CGTATGC | 325 | 0.0 | 31.230768 | 41 |
CGGATCA | 45 | 0.0042781457 | 31.111113 | 14 |
ACTACGT | 45 | 0.0042781457 | 31.111113 | 3 |