##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780616_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63495 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.890007087172215 34.0 34.0 34.0 31.0 34.0 2 33.279029844869676 34.0 34.0 34.0 32.0 34.0 3 33.49222773446728 34.0 34.0 34.0 34.0 34.0 4 33.539239310181905 34.0 34.0 34.0 34.0 34.0 5 33.543649106228834 34.0 34.0 34.0 34.0 34.0 6 37.27739192062367 38.0 38.0 38.0 36.0 38.0 7 36.941759193637296 38.0 37.0 38.0 35.0 38.0 8 36.91846602094653 38.0 37.0 38.0 35.0 38.0 9 36.74114497204504 38.0 37.0 38.0 34.0 38.0 10-11 36.474108197495866 38.0 37.0 38.0 34.0 38.0 12-13 36.34206630443342 38.0 37.0 38.0 34.0 38.0 14-15 36.592676588707775 38.0 37.0 38.0 34.0 38.0 16-17 36.707228915662654 38.0 37.0 38.0 34.0 38.0 18-19 36.73643593983778 38.0 37.0 38.0 34.0 38.0 20-21 36.47248602252146 38.0 37.0 38.0 34.0 38.0 22-23 36.54963382943539 38.0 37.0 38.0 34.0 38.0 24-25 35.90875659500748 38.0 36.5 38.0 32.5 38.0 26-27 36.115757146231985 38.0 37.0 38.0 33.5 38.0 28-29 35.98014016851721 38.0 37.0 38.0 33.0 38.0 30-31 35.6809906291834 38.0 36.5 38.0 31.5 38.0 32-33 36.190329947239945 38.0 37.0 38.0 34.0 38.0 34-35 36.248240018899125 38.0 37.0 38.0 34.0 38.0 36-37 36.34565713835735 38.0 37.0 38.0 34.0 38.0 38-39 35.96536735175998 38.0 36.5 38.0 32.5 38.0 40-41 35.545499645641385 38.0 36.0 38.0 31.0 38.0 42-43 35.9648869989763 38.0 36.5 38.0 32.5 38.0 44-45 36.128388062052124 38.0 37.0 38.0 34.0 38.0 46-47 36.40896921017403 38.0 37.0 38.0 34.0 38.0 48-49 36.21491456020159 38.0 37.0 38.0 34.0 38.0 50-51 36.38458146310733 38.0 37.0 38.0 34.0 38.0 52-53 36.286959603118355 38.0 37.0 38.0 34.0 38.0 54-55 36.42755335065753 38.0 37.0 38.0 34.0 38.0 56-57 36.47538388849516 38.0 37.0 38.0 34.0 38.0 58-59 36.5375856366643 38.0 37.0 38.0 34.0 38.0 60-61 36.2512560044098 38.0 37.0 38.0 33.5 38.0 62-63 36.50944956295771 38.0 37.0 38.0 34.0 38.0 64-65 36.536829671627686 38.0 37.0 38.0 34.0 38.0 66-67 35.94057799826758 38.0 37.0 38.0 32.0 38.0 68-69 35.34902748247894 38.0 36.0 38.0 29.0 38.0 70-71 35.314843688479414 38.0 36.0 38.0 30.0 38.0 72-73 34.97716355618553 38.0 36.0 38.0 26.0 38.0 74-75 35.548232144263324 38.0 36.5 38.0 31.0 38.0 76 34.980187416331994 38.0 36.0 38.0 27.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5402606490313815 1101 2 0.6088983776163417 1101 3 0.3353846439868491 1101 4 0.4314282575266901 1101 5 0.09343288147825035 1101 6 -0.5932076258628101 1101 7 0.0466944094600521 1101 8 0.5769363347817844 1101 9 0.5032376051900869 1101 10-11 0.08253541758162442 1101 12-13 0.8290493569258004 1101 14-15 0.497091142551362 1101 16-17 0.010724509275192418 1101 18-19 -0.2204457202269623 1101 20-21 0.32705925118673207 1101 22-23 0.8092269712469999 1101 24-25 -1.343147619899959 1101 26-27 -0.038591395189982336 1101 28-29 0.6965698303145587 1101 30-31 1.129844644862004 1101 32-33 0.07344253555123714 1101 34-35 0.5766823256898519 1101 36-37 0.4120094253457438 1101 38-39 0.10299883677284782 1101 40-41 1.2262930084468948 1101 42-43 0.7017549622163202 1101 44-45 0.6043584952481567 1101 46-47 0.7832797658686701 1101 48-49 -1.3295832788286077 1101 50-51 -0.9873051070167875 1101 52-53 -1.7807194051120376 1101 54-55 -0.2937869506753046 1101 56-57 -1.0190929690751531 1101 58-59 -1.1626778741838777 1101 60-61 -1.91371925672032 1101 62-63 -1.0380202651371349 1101 64-65 -0.9126290695168962 1101 66-67 -1.5196718530098607 1101 68-69 -0.7756876770448571 1101 70-71 -0.4146935743645557 1101 72-73 -1.4246568273922406 1101 74-75 -1.024778045463016 1101 76 -1.6331439602495692 1102 1 -1.3777164189456848 1102 2 -0.09441857570061529 1102 3 -0.1502418916396877 1102 4 0.42299254909098494 1102 5 0.17950986755523246 1102 6 0.28054339788821636 1102 7 0.4054577182233601 1102 8 0.5867233945688426 1102 9 0.4949656969181788 1102 10-11 0.5511881612343714 1102 12-13 0.3649011177775563 1102 14-15 -0.7334166379564167 1102 16-17 0.09788667143735097 1102 18-19 -0.5040042787855157 1102 20-21 0.4275301766576547 1102 22-23 0.2504227124427416 1102 24-25 -4.365158266910605 1102 26-27 -1.172579929178518 1102 28-29 -0.31417955543482634 1102 30-31 1.3764048414221932 1102 32-33 0.12010510721380996 1102 34-35 0.22657995058747815 1102 36-37 -2.6301895918532807 1102 38-39 -0.5575662737922684 1102 40-41 0.7086844908383796 1102 42-43 0.21872875419010995 1102 44-45 0.5861562020458635 1102 46-47 0.9311503637392633 1102 48-49 -2.967625866871188 1102 50-51 -0.658578653290327 1102 52-53 -0.5689462683388982 1102 54-55 0.435062353173997 1102 56-57 0.5446457696635818 1102 58-59 -0.6033003148063187 1102 60-61 -5.487818355819421 1102 62-63 -2.1399039670208353 1102 64-65 -0.1336978655856953 1102 66-67 0.5050210216830138 1102 68-69 -0.5706712970284684 1102 70-71 -0.8141407488117309 1102 72-73 -5.45704830978373 1102 74-75 -2.0790777997627714 1102 76 -0.7155354426410554 1103 1 0.4493860665837701 1103 2 0.5439879615823315 1103 3 -0.10805598765603008 1103 4 0.3518655487505029 1103 5 0.14369100477007635 1103 6 0.2838801360564176 1103 7 0.7072622807020821 1103 8 0.6567267994037138 1103 9 0.20715500573670198 1103 10-11 0.4937485970532336 1103 12-13 -0.37353946785403735 1103 14-15 -0.13394438736056458 1103 16-17 0.07597655907780165 1103 18-19 0.5941230620384488 1103 20-21 -0.15498599630795695 1103 22-23 0.4275081737978681 1103 24-25 -2.5741300067812247 1103 26-27 -0.12756801225648928 1103 28-29 0.14726068971328488 1103 30-31 1.1908072929033011 1103 32-33 1.0664959822563702 1103 34-35 0.6951533247788362 1103 36-37 -0.5036829524252937 1103 38-39 0.22302616747433746 1103 40-41 -0.3152344740918167 1103 42-43 -0.2519726417921788 1103 44-45 1.1847942680996582 1103 46-47 0.9351169963575785 1103 48-49 -1.7282319274772533 1103 50-51 -0.11397531655216397 1103 52-53 0.182194351341046 1103 54-55 -0.3019482018140849 1103 56-57 0.3399641217235043 1103 58-59 -0.3168685817453678 1103 60-61 -4.266095509377472 1103 62-63 -0.8795021965067065 1103 64-65 0.4849946778259522 1103 66-67 0.11242653751548914 1103 68-69 0.5653007833256325 1103 70-71 0.7759001092066597 1103 72-73 -4.804477659460268 1103 74-75 -1.8692208028271153 1103 76 -3.3247625451265854 1104 1 0.21153657612126864 1104 2 0.30515859131841694 1104 3 0.36476867918484146 1104 4 -0.1309538920647597 1104 5 -0.13472550947084017 1104 6 0.18177666074938514 1104 7 0.580792460999966 1104 8 0.8278791380501715 1104 9 0.9092715461077461 1104 10-11 -0.23864622918141976 1104 12-13 0.30783628338713953 1104 14-15 0.7473268433684623 1104 16-17 0.08567736911177093 1104 18-19 0.6638251785788256 1104 20-21 -0.2005177444135171 1104 22-23 0.17982722266120987 1104 24-25 0.19817859003089922 1104 26-27 0.8319302892851894 1104 28-29 1.219118541816762 1104 30-31 1.1506648837054883 1104 32-33 0.6450170170094367 1104 34-35 0.4038350598193361 1104 36-37 1.1585524800283338 1104 38-39 1.352370301260585 1104 40-41 1.7378423484846124 1104 42-43 0.6534362947116108 1104 44-45 0.6860681118414931 1104 46-47 0.7578240776222813 1104 48-49 0.010140165546005164 1104 50-51 0.6938702543911361 1104 52-53 0.16939058860260303 1104 54-55 0.24015396698654 1104 56-57 0.13921941307443575 1104 58-59 -0.6670776227696678 1104 60-61 0.3398948224751521 1104 62-63 0.45019469400805434 1104 64-65 0.31772919572509295 1104 66-67 0.2844607679832194 1104 68-69 1.1560376529362912 1104 70-71 1.5157746845920812 1104 72-73 0.9184449953607441 1104 74-75 0.009241439137866791 1104 76 0.03844623459643515 1105 1 0.7280984868692215 1105 2 0.4534929722109311 1105 3 0.20160086020306522 1105 4 0.0071039332023659085 1105 5 -0.22818874014337354 1105 6 0.16490048224530085 1105 7 0.6858835986492409 1105 8 0.09990930775475704 1105 9 0.8302646322171157 1105 10-11 0.6886164986627037 1105 12-13 -0.15270739983095893 1105 14-15 0.6930370884973129 1105 16-17 0.5032920768427545 1105 18-19 0.8788786040973662 1105 20-21 0.4534106025380851 1105 22-23 0.6389567009767276 1105 24-25 0.9386091368568046 1105 26-27 0.5533004967019082 1105 28-29 0.860759019503746 1105 30-31 1.2230878881052405 1105 32-33 1.088983076091779 1105 34-35 -0.07399334998581963 1105 36-37 0.6572796956160118 1105 38-39 0.6962420800160913 1105 40-41 0.9479146300685173 1105 42-43 0.6754036108649686 1105 44-45 0.8631422790319405 1105 46-47 0.9765230091119079 1105 48-49 0.5312275319822106 1105 50-51 1.044451649739976 1105 52-53 0.7537400543474249 1105 54-55 0.979861697973341 1105 56-57 0.6518529768707921 1105 58-59 0.615700504194507 1105 60-61 1.0356050676040027 1105 62-63 0.094412167295296 1105 64-65 0.8002087683209425 1105 66-67 0.38167949834149084 1105 68-69 0.14660962025244828 1105 70-71 1.106252371581398 1105 72-73 0.9442620915266744 1105 74-75 0.03197871129374619 1105 76 0.3776668505612406 1106 1 -0.9180368204125813 1106 2 -0.0724151014678398 1106 3 0.2495264928420724 1106 4 -0.07270326252839254 1106 5 -3.0097977153786815E-4 1106 6 0.13122859379634377 1106 7 -1.1662079833786478 1106 8 0.5486612444047836 1106 9 -0.753519151566671 1106 10-11 0.11698626970763115 1106 12-13 0.9031369795802959 1106 14-15 -0.5255031077498913 1106 16-17 0.27877839118257697 1106 18-19 0.39005092291299803 1106 20-21 -0.025432571846494056 1106 22-23 0.4379926796305469 1106 24-25 -0.549099590979317 1106 26-27 0.47827897836828726 1106 28-29 0.354320734084574 1106 30-31 0.6340192015843229 1106 32-33 -0.10216858512358584 1106 34-35 0.7071049457621612 1106 36-37 1.112022336345916 1106 38-39 -0.09311925584206193 1106 40-41 0.824588756424177 1106 42-43 0.7977482484962337 1106 44-45 0.7454111721288612 1106 46-47 0.7377900085954678 1106 48-49 1.093424123478158 1106 50-51 -0.6389878407058873 1106 52-53 -0.048428993681497445 1106 54-55 -0.11025708322824102 1106 56-57 0.18965638537483898 1106 58-59 0.5234126749639927 1106 60-61 0.7979565911020217 1106 62-63 0.6044311034288867 1106 64-65 -1.1675137312486896 1106 66-67 0.3262138416019482 1106 68-69 1.2969797355907104 1106 70-71 -0.1371285889970224 1106 72-73 0.26207927176423595 1106 74-75 -0.4021235438276207 1106 76 -0.05628047255805768 1107 1 0.6934627641900235 1107 2 -0.4936280618666231 1107 3 -0.012000784920317642 1107 4 -0.060933669617845965 1107 5 -0.48191964496123774 1107 6 -0.006544876156581836 1107 7 0.5700492860758004 1107 8 -0.46521995193971577 1107 9 -0.5252582820014524 1107 10-11 0.7300089780986596 1107 12-13 -0.1055863657534033 1107 14-15 0.2976933752188202 1107 16-17 0.6701692801330097 1107 18-19 0.4229884748376733 1107 20-21 0.7897502030081185 1107 22-23 0.16447080203430886 1107 24-25 0.27729891359005165 1107 26-27 -0.02449033761066488 1107 28-29 0.9822631323122124 1107 30-31 0.103331718892548 1107 32-33 0.45505240629153576 1107 34-35 -0.12220134058511434 1107 36-37 0.6789013172376315 1107 38-39 -0.17777378367368613 1107 40-41 0.04547632219543374 1107 42-43 0.7739494393021005 1107 44-45 0.3167556750366387 1107 46-47 0.492151681262321 1107 48-49 0.7581811160010119 1107 50-51 0.715103823653017 1107 52-53 0.4550326512921927 1107 54-55 0.09619542300271888 1107 56-57 0.3509863493737342 1107 58-59 0.6490876957338685 1107 60-61 0.8331961369341983 1107 62-63 0.3041507101860148 1107 64-65 0.1630906719637153 1107 66-67 -0.4233880692260783 1107 68-69 1.1734163182547945 1107 70-71 0.03808257134637216 1107 72-73 0.639312032772267 1107 74-75 -0.9648485507509079 1107 76 -0.05558814356331965 1108 1 -0.20927336774401084 1108 2 0.36744140340074694 1108 3 0.06752195371036862 1108 4 0.45705733487990585 1108 5 0.13071153944655833 1108 6 0.06142114083492345 1108 7 0.26376492825470876 1108 8 -0.8473413912200769 1108 9 -0.4489214504172452 1108 10-11 0.3281474088832752 1108 12-13 1.041964858634401 1108 14-15 0.6316826305911292 1108 16-17 0.09673724614999912 1108 18-19 0.37185779018000176 1108 20-21 1.095659748235505 1108 22-23 0.12827015235914985 1108 24-25 1.1311844708114407 1108 26-27 -0.302726219669637 1108 28-29 -0.9471471625403609 1108 30-31 -0.026259735380314453 1108 32-33 0.6057895377258191 1108 34-35 0.6583458858016868 1108 36-37 1.2306254551686635 1108 38-39 1.1301035276706415 1108 40-41 1.5425402902114271 1108 42-43 0.13779876463943452 1108 44-45 0.6827601727877664 1108 46-47 0.7301576782638222 1108 48-49 0.9298140495342437 1108 50-51 1.0065206152572088 1108 52-53 -0.15191387544444268 1108 54-55 -0.08358968444355952 1108 56-57 0.2756802524222053 1108 58-59 0.3805032379627562 1108 60-61 1.0949402648702318 1108 62-63 1.0944742983919085 1108 64-65 1.0184442451771076 1108 66-67 0.8072153790497794 1108 68-69 1.3894645441418518 1108 70-71 -0.1321135805776592 1108 72-73 -0.1029730746740114 1108 74-75 -0.19548323340957552 1108 76 1.2345168039629186 1109 1 -0.5581464172527788 1109 2 0.3829811195761792 1109 3 0.3981583115091212 1109 4 0.49808989122742986 1109 5 -0.038908957567500124 1109 6 -0.21094128130596346 1109 7 -0.1458542844852886 1109 8 0.7317377842759711 1109 9 0.6543020774836066 1109 10-11 0.5137410645693947 1109 12-13 0.7077485868820119 1109 14-15 0.6401909462656974 1109 16-17 0.7671604144541107 1109 18-19 0.7235562426297548 1109 20-21 0.9635721607443344 1109 22-23 0.318663782359792 1109 24-25 0.1392432905635772 1109 26-27 -0.10525302889072918 1109 28-29 -0.8088468992641822 1109 30-31 0.27498279977669426 1109 32-33 0.5311090008992636 1109 34-35 -0.2623822975926444 1109 36-37 0.9833705909806483 1109 38-39 1.020747591113782 1109 40-41 0.9686303178233189 1109 42-43 0.7357086086001345 1109 44-45 0.630120989586068 1109 46-47 0.6120205177937805 1109 48-49 0.8918798043663259 1109 50-51 -0.08739192567902165 1109 52-53 -0.35951677790382774 1109 54-55 -0.4925828135773145 1109 56-57 0.3493012631458967 1109 58-59 0.011563406202654392 1109 60-61 0.8291276404561856 1109 62-63 0.7315705547330182 1109 64-65 0.9684859840841895 1109 66-67 0.2124359531202913 1109 68-69 -0.6238116389956332 1109 70-71 0.15787550309278942 1109 72-73 -0.08459190213179113 1109 74-75 -0.38979389930568686 1109 76 0.1524516912678635 1110 1 -0.8642464592329517 1110 2 -0.5999361794841107 1110 3 -0.4205519284237127 1110 4 -0.034670789497212695 1110 5 0.3600318503893547 1110 6 -0.3527373868376529 1110 7 0.3635589618737356 1110 8 0.4883173689951903 1110 9 0.7724924091269685 1110 10-11 -0.5143049198656513 1110 12-13 0.4458084570663985 1110 14-15 0.9391705051509618 1110 16-17 0.19962077548359503 1110 18-19 0.03433868845918653 1110 20-21 0.8299298963984114 1110 22-23 -0.39378818263520543 1110 24-25 -0.628864695356917 1110 26-27 0.9679700795795867 1110 28-29 0.10804850552155898 1110 30-31 -0.2822394045052903 1110 32-33 0.7004344118205665 1110 34-35 0.6893405040994764 1110 36-37 1.2742770397809835 1110 38-39 0.6394384555824075 1110 40-41 -1.34736735712098 1110 42-43 0.04586135184294449 1110 44-45 0.6583930211034925 1110 46-47 0.7222346166732336 1110 48-49 1.2573172054707271 1110 50-51 -0.1763575988149384 1110 52-53 0.42977504887952733 1110 54-55 -0.7839252103222378 1110 56-57 0.23385578893140746 1110 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38-39 -3.8398685868638083 2116 40-41 -1.018229620691116 2116 42-43 -1.3120584073662798 2116 44-45 -0.3650361953003838 2116 46-47 -1.1772591754395023 2116 48-49 -0.11580589197428992 2116 50-51 -1.1425305987807377 2116 52-53 -0.3698966000584605 2116 54-55 -0.20365021630779978 2116 56-57 -0.04785916130288825 2116 58-59 -2.1251758751434124 2116 60-61 -1.1590215116379596 2116 62-63 0.16524216889452958 2116 64-65 -1.7511265907067326 2116 66-67 -0.7344087792852463 2116 68-69 -1.1615624571503957 2116 70-71 0.8240518278423892 2116 72-73 0.3598066301481353 2116 74-75 -0.2322784452711062 2116 76 -1.3057810944251713 2117 1 0.6103882300285335 2117 2 0.5250054785060527 2117 3 0.4670364821604238 2117 4 0.14092968441942588 2117 5 -0.01996312322210514 2117 6 -0.2021970500696213 2117 7 0.10498717427455517 2117 8 0.13338253253233034 2117 9 -0.7969974124362338 2117 10-11 -0.9586556181746317 2117 12-13 -1.4937851235173838 2117 14-15 -0.24694948192404098 2117 16-17 -0.7083245086868715 2117 18-19 -0.9267786059076712 2117 20-21 -0.10077774730244471 2117 22-23 -0.14880559548121397 2117 24-25 -2.284036490136657 2117 26-27 -1.8412605239460689 2117 28-29 -1.489786557128781 2117 30-31 -0.11444778421304136 2117 32-33 -1.5069041154475897 2117 34-35 0.383233442023581 2117 36-37 -1.140974459159878 2117 38-39 0.6549125517300425 2117 40-41 0.26674961412089004 2117 42-43 0.07713266911576966 2117 44-45 -0.5924058156465932 2117 46-47 -1.0389042193587983 2117 48-49 -0.9673623622598626 2117 50-51 -0.535672573862513 2117 52-53 0.43329352085741135 2117 54-55 -0.45349401799106914 2117 56-57 -0.17191737733434564 2117 58-59 -0.37847441606737675 2117 60-61 -0.1279839872894044 2117 62-63 -0.15897040782640914 2117 64-65 -0.8147819988437419 2117 66-67 -0.3026582555614823 2117 68-69 0.37877346111194043 2117 70-71 0.056327912961286586 2117 72-73 1.3407871880517774 2117 74-75 1.1034744306155488 2117 76 0.9909957073683557 2118 1 0.09529111302138915 2118 2 0.4636476355447883 2118 3 -0.26448256426186134 2118 4 0.3121500197513427 2118 5 0.4004942781350209 2118 6 0.2252972952432657 2118 7 0.5311890196772069 2118 8 0.36693008575819874 2118 9 -0.27375036543950415 2118 10-11 -0.46806214529917867 2118 12-13 -0.635116398424934 2118 14-15 0.7802109675787037 2118 16-17 -2.106737606019294 2118 18-19 -0.41074801674312766 2118 20-21 -0.022671259670950406 2118 22-23 0.794073089041099 2118 24-25 1.1399214318107127 2118 26-27 -1.6447466834261917 2118 28-29 0.07336642459669918 2118 30-31 -0.2186555894763842 2118 32-33 -0.010548247138963518 2118 34-35 -0.1748213456115053 2118 36-37 -2.231503307039823 2118 38-39 0.379322848645991 2118 40-41 -0.4456332530747389 2118 42-43 0.5921041264094171 2118 44-45 -1.5092370366999788 2118 46-47 0.0921768132050147 2118 48-49 0.5376640262799697 2118 50-51 -0.41046164505771543 2118 52-53 -0.6106873788661389 2118 54-55 -0.3128269444262841 2118 56-57 0.45061879471175104 2118 58-59 -0.440837536736602 2118 60-61 -0.32230775851692073 2118 62-63 -1.1130866610127796 2118 64-65 0.1917882090333265 2118 66-67 0.5632448787508011 2118 68-69 -0.9216609624690051 2118 70-71 0.2795534182928421 2118 72-73 1.7255931335513566 2118 74-75 0.6649579332903102 2118 76 0.9116146712142594 2119 1 0.6779945367652758 2119 2 -0.6544418857239265 2119 3 0.04917535777756399 2119 4 -0.14064381454537767 2119 5 0.022400310445675586 2119 6 -0.3788971615523451 2119 7 -0.19291058014493956 2119 8 0.2503597582052066 2119 9 -0.16377556182307984 2119 10-11 -0.007727922681716848 2119 12-13 0.20266335553979076 2119 14-15 0.04845981892004403 2119 16-17 -0.2213440977934198 2119 18-19 0.07676495198371924 2119 20-21 -0.9042297301022515 2119 22-23 -0.0332019961819654 2119 24-25 0.9273825256301862 2119 26-27 0.81553195893337 2119 28-29 0.24759200633673828 2119 30-31 1.271043536060887 2119 32-33 0.5655596526683482 2119 34-35 0.6584564074639374 2119 36-37 0.23018336851968968 2119 38-39 -1.5104800267060128 2119 40-41 -0.24061620846232046 2119 42-43 0.3602788707402311 2119 44-45 0.31494408083374736 2119 46-47 0.18097553856443938 2119 48-49 -1.2442575685028956 2119 50-51 0.538682418970744 2119 52-53 0.1646201652275323 2119 54-55 0.4206101387217842 2119 56-57 -1.4085593585415452 2119 58-59 -0.2775427390487408 2119 60-61 -0.3205106635117332 2119 62-63 -0.3340181861350615 2119 64-65 -0.14634355323138237 2119 66-67 0.1903939820559728 2119 68-69 0.48981821346103516 2119 70-71 -0.18063259030356704 2119 72-73 0.7569959792605658 2119 74-75 0.21101544033047048 2119 76 -0.5252091023147116 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 7.0 23 12.0 24 24.0 25 67.0 26 92.0 27 168.0 28 292.0 29 534.0 30 844.0 31 1347.0 32 2143.0 33 3336.0 34 5202.0 35 8150.0 36 14350.0 37 26924.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.56185526419403 23.176628080951257 12.256083156154029 29.005433498700683 2 17.317899047169067 19.360579573194737 39.032994723994015 24.288526655642176 3 16.439089692101742 25.861878888101426 31.284353098669186 26.41467832112765 4 12.682888416410739 20.03779825183085 39.90550437042287 27.373808961335538 5 11.709583431766282 37.958894401133946 38.1636349318844 12.167887235215371 6 26.099692889203872 41.187495078352626 18.10693755413812 14.605874478305378 7 25.674462556106782 30.202378140011028 24.30112607291913 19.82203323096307 8 22.500984329474765 37.37617135207497 20.985904401921413 19.13693991652886 9 23.512087565950075 14.953933380581146 22.313567997480117 39.22041105598866 10-11 24.41058351051264 26.260335459485002 27.905346877706904 21.423734152295456 12-13 23.420741790692183 23.018347901409562 27.31868651074888 26.242223797149382 14-15 21.493030947318687 24.942121426884007 23.4223167178518 30.14253090794551 16-17 22.77187180092921 28.308528230569337 25.163398692810457 23.756201275690998 18-19 22.593117568312465 26.754075124025512 26.567446255610676 24.085361052051343 20-21 21.36624931096937 26.957240727616348 25.967399007795887 25.709110953618396 22-23 22.067091896999763 28.051027639971654 26.4690133081345 23.412867154894084 24-25 22.797858099062918 28.337664383022286 26.398141585951652 22.466335931963147 26-27 22.05764233404205 28.756595007480907 26.695802819119617 22.48995983935743 28-29 23.063233325458697 26.466650917395068 27.36987164343649 23.100244113709742 30-31 22.575793369556656 28.12111189857469 26.4690133081345 22.83408142373415 32-33 21.29695251594614 28.270729978738483 27.11000866209938 23.322308843216003 34-35 22.99078667611623 26.851720607921887 27.191904874399558 22.965587841562325 36-37 21.983620757539963 27.427356484762576 27.734467280888257 22.854555476809196 38-39 21.74108197495866 27.006850933144342 28.0218914875187 23.230175604378296 40-41 21.144972045042916 26.284746830459092 29.04323175053154 23.527049373966456 42-43 22.520670918969998 25.709898417198207 29.169225923301045 22.60020474053075 44-45 20.53862508858965 26.628868414835814 29.411764705882355 23.420741790692183 46-47 21.926923379793685 25.223245924875975 28.71800929207024 24.131821403260098 48-49 20.759114890936296 25.803606583195528 29.21804866524923 24.219229860618945 50-51 19.82833293960154 25.511457595086227 29.512560044097963 25.14764942121427 52-53 19.462162374990157 25.917788802267893 31.393810536262695 23.22623828647925 54-55 18.656587132845107 26.217812426175293 30.211827702968737 24.913772738010866 56-57 20.30789825970549 25.706748562878968 28.728246318607763 25.25710685880778 58-59 19.16922592330105 26.39262934089298 30.143318371525318 24.294826364280652 60-61 20.237026537522638 27.83526261910387 27.794314512953775 24.133396330419718 62-63 18.723521537128907 29.268446334357034 27.015513032522247 24.99251909599181 64-65 18.892038743208126 30.510276399716513 25.59020395306717 25.00748090400819 66-67 18.792818332152137 30.378769981888336 25.332703362469484 25.495708323490042 68-69 19.062130876446965 29.830695330340973 25.458697535238993 25.64847625797307 70-71 19.76297346247736 29.36136703677455 25.01220568548705 25.863453815261046 72-73 20.34569651153634 28.710134656272146 24.277502165524844 26.666666666666668 74-75 20.564611386723364 28.530592960075595 24.323175053153793 26.581620600047245 76 20.563823923143552 28.551854476730455 24.4491692259233 26.43515237420269 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.5 14 1.5 15 1.5 16 1.5 17 2.0 18 2.5 19 7.0 20 10.0 21 9.0 22 23.0 23 42.0 24 43.5 25 40.0 26 101.0 27 172.5 28 243.5 29 304.0 30 365.0 31 521.5 32 754.5 33 892.0 34 1092.5 35 1426.5 36 1683.0 37 1806.0 38 2127.5 39 2847.0 40 3482.0 41 4145.0 42 4571.0 43 4793.5 44 5134.0 45 5154.5 46 5057.0 47 5129.5 48 4839.5 49 4115.5 50 3754.0 51 3491.5 52 2861.5 53 2283.5 54 2073.0 55 1919.5 56 1503.5 57 1074.5 58 908.0 59 707.0 60 440.0 61 311.5 62 249.0 63 222.5 64 163.5 65 114.0 66 73.5 67 50.0 68 38.0 69 15.5 70 5.5 71 6.0 72 4.5 73 1.5 74 1.0 75 2.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 63495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.774706669816524 #Duplication Level Percentage of deduplicated Percentage of total 1 57.57514216084484 22.32459248759745 2 15.609260763606825 12.104890148830616 3 8.40373679935012 9.77557287975431 4 5.219333874898457 8.09512560044098 5 3.172217709179529 6.150090558311678 6 2.432981316003249 5.66028821167021 7 1.7465475223395612 4.740530750452791 8 1.3119415109666939 4.069611780455154 9 0.8529650690495532 2.97661233167966 >10 3.6555645816409426 21.189070005512246 >50 0.004061738424045491 0.13229388140798487 >100 0.012185215272136474 1.1481218993621545 >500 0.0 0.0 >1k 0.004061738424045491 1.6331994645247656 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT 1037 1.6331994645247656 No Hit ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT 446 0.7024175131900149 TruSeq Adapter, Index 2 (95% over 21bp) CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC 172 0.27088747145444525 No Hit ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 111 0.1748169147176943 No Hit TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG 84 0.13229388140798487 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0031498543192377353 0.0 2 0.0 0.0 0.0 0.009449562957713206 0.0 3 0.0 0.0 0.0 0.017324198755807544 0.0 4 0.0 0.0 0.0 0.025198834553901883 0.0 5 0.0 0.0 0.0 0.025198834553901883 0.0 6 0.0 0.0 0.0 0.025198834553901883 0.0 7 0.0 0.0 0.0 0.03307347035199622 0.0 8 0.0 0.0 0.0 0.03307347035199622 0.0 9 0.0 0.0 0.0 0.07087172218284904 0.0 10 0.0 0.0 0.0 0.09607055673675093 0.0 11 0.0 0.0 0.0 0.09922041105598867 0.0 12 0.0 0.0 0.0 0.1023702653752264 0.0 13 0.0 0.0 0.0 0.1023702653752264 0.0 14 0.0 0.0 0.0 0.10552011969446413 0.0 15 0.0 0.0 0.0 0.107095046854083 0.0 16 0.0 0.0 0.0 0.11024490117332074 0.0 17 0.0 0.0 0.0 0.12284431845027168 0.0 18 0.0 0.0 0.0 0.13701866288684147 0.0 19 0.0 0.0 0.0 0.1511930073234113 0.0 20 0.0 0.0 0.0 0.15749271596188677 0.0 21 0.0 0.0 0.0 0.16694227891959998 0.0 22 0.0 0.0 0.0 0.17954169619655092 0.0 23 0.0 0.0 0.0 0.18584140483502637 0.0 24 0.0 0.0 0.0 0.19056618631388297 0.0 25 0.0 0.0 0.0 0.19371604063312073 0.0 26 0.0 0.0 0.0 0.20789038506969051 0.0 27 0.0 0.0 0.0 0.24253878258130562 0.0 28 0.0 0.0 0.0 0.27403732577368295 0.0 29 0.0 0.0 0.0 0.31498543192377354 0.0 30 0.0 0.0 0.0 0.34333412079691317 0.0 31 0.0 0.0 0.0 0.39058193558547916 0.0 32 0.0 0.0 0.0 0.428380187416332 0.0 33 0.0 0.0 0.0 0.4724781478856603 0.0 34 0.0 0.0 0.0 0.5323253799511772 0.0 35 0.0 0.0 0.0 0.6362705724860225 0.0 36 0.0 0.0 0.0 0.7795889440113395 0.0 37 0.0 0.0 0.0 1.2095440585872903 0.0 38 0.0 0.0 0.0 1.6741475706748563 0.0 39 0.0 0.0 0.0 2.190723679029845 0.0 40 0.0 0.0 0.0 2.833293960154343 0.0 41 0.0 0.0 0.0 3.592408851090637 0.0 42 0.0 0.0 0.0 4.3436491062288365 0.0 43 0.0 0.0 0.0 5.170485865028742 0.0 44 0.0 0.0 0.0 5.991022915190173 0.0 45 0.0 0.0 0.0 6.800535475234271 0.0 46 0.0 0.0 0.0 7.474604299551146 0.0 47 0.0 0.0 0.0 8.232144263327822 0.0 48 0.0 0.0 0.0 9.029057406094967 0.0 49 0.0 0.0 0.0 9.773997952594693 0.0 50 0.0 0.0 0.0 10.51578864477518 0.0 51 0.0 0.0 0.0 11.298527443105757 0.0 52 0.0 0.0 0.0 12.097015513032522 0.0 53 0.0 0.0 0.0 12.83723127805339 0.0 54 0.0 0.0 0.0 13.541223718403025 0.0 55 0.0 0.0 0.0 14.150720529175526 0.0 56 0.0 0.0 0.0 14.775966611544217 0.0 57 0.0 0.0 0.0 15.352389951964721 0.0 58 0.0 0.0 0.0 15.972911252854555 0.0 59 0.0 0.0 0.0 16.60445704386172 0.0 60 0.0 0.0 0.0 17.264351523742025 0.0 61 0.0 0.0 0.0 17.807701393810536 0.0 62 0.0 0.0 0.0 18.333727065123238 0.0 63 0.0 0.0 0.0 18.895976061107174 0.0 64 0.0 0.0 0.0 19.425151586739112 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGAG 20 0.0069219554 52.5 15 TTGGCGT 20 0.0069219554 52.5 23 ACATTAT 20 0.0069219554 52.5 12 AGCTATG 20 0.0069219554 52.5 12 CACTTCC 20 0.0069219554 52.5 17 AGGACGG 35 0.001246825 40.0 10 GATCAGA 35 0.001246825 40.0 16 GACGAAG 35 0.001246825 40.0 21 CGAAGAG 35 0.001246825 40.0 23 ACGAAGA 35 0.001246825 40.0 22 GGGCGTT 40 0.0024023054 35.0 30 TTAAAGC 40 0.0024023054 35.0 45 TAGGACG 40 0.0024023054 35.0 9 CGTTTGG 40 0.0024023054 35.0 33 TCTCGTA 285 0.0 33.157894 38 ATCTCGT 295 0.0 32.033897 37 CTTCACC 55 2.9619178E-4 31.81818 9 CGTATGC 325 0.0 31.230768 41 CGGATCA 45 0.0042781457 31.111113 14 ACTACGT 45 0.0042781457 31.111113 3 >>END_MODULE