FastQCFastQC Report
Wed 25 May 2016
SRR1780615_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780615_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557637
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCA451.0496697E-634.1644063
AATCGTT602.9194234E-729.35748739
TCTGGAG300.00573190829.34429610
CTTATTG300.005772297729.3021681
TGAGTCG454.1128253E-529.2837778
TGCAAAT300.00579003929.2837732
TCGAGTC407.0600223E-427.4781833
CGTTGAA655.967431E-727.05300942
ATTCGTT508.2212915E-526.4526520
CGACAAT1250.026.43361717
CGAAATC508.266708E-526.43124213
TCGAAAT508.281894E-526.42411212
GTCGAAA508.312337E-526.40986611
TTTTACG508.363287E-526.38615629
TTACGTT508.378623E-526.37905131
ATTGGCG801.2968121E-724.77037837
TGTTCGT801.302451E-724.75924910
CGCCATT801.3172576E-724.73036233
CGCTACG450.001382589224.48878916
TCGCACG450.001394228424.45357910
Produced by FastQC (version 0.11.5)