FastQCFastQC Report
Wed 25 May 2016
SRR1780615_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780615_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences557637
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT139892.508621199812781No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT46590.8354897540873364RNA PCR Primer, Index 34 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC25050.4492169637237127No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG13190.23653380245571942No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC12030.21573173946492072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGGG207.8450335E-444.00905611
ATCGTAT254.4415377E-543.9972239
ATATCGT207.855436E-443.9972237
TACGCGT207.855436E-443.9972227
ATACGCG207.855436E-443.9972226
GAATTCG404.1215026E-738.49756623
TTCGAAC404.1215026E-738.49756626
TCGAACA404.1215026E-738.49756627
CCGTCTT5350.035.3622544
TGCGTTT250.00234933535.2072513
CCACTCG250.002351403535.2009359
TGGGATA250.002352438235.19777733
GCCGTCC250.002352438235.19777737
TTCGGTA451.0363965E-634.22006226
GCCGTCT5650.033.09525343
GGGCTCG401.8168786E-533.0038316
CACTCGG353.2117762E-431.43504110
TAACGGT353.2168487E-431.42658436
GTTAACG353.2168487E-431.42658434
TTGTTCT353.2168487E-431.42658438
Produced by FastQC (version 0.11.5)