FastQCFastQC Report
Wed 25 May 2016
SRR1780614_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780614_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81040
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAAGT150.002218200869.9938316
GCCGAGA207.8877245E-569.9938312
GGCCGAG308.314619E-658.3281911
GATATTC308.314619E-658.3281949
ATATTCG308.314619E-658.3281950
TACGAGT252.3787828E-455.9950644
CGAGTAC200.006931264452.495376
TCGGGCC200.006931264452.4953741
GGTCAGA200.006931264452.4953749
AGTGGCA200.006931264452.4953712
GGCATTA200.006931264452.4953744
CGACTAC200.006931264452.4953715
ACGAGTA200.006931264452.495375
TGTGGGA352.0712518E-549.99559425
GAATATG352.0712518E-549.99559454
GGAAGAA352.0712518E-549.99559429
AGGCCGA352.0712518E-549.99559410
ACGCCGA305.84946E-446.6625546
TAAAGTA305.84946E-446.6625515
AAGATAT305.84946E-446.6625547
Produced by FastQC (version 0.11.5)