##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780614_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81040 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.486266041461008 34.0 27.0 34.0 12.0 34.0 2 31.06419052319842 34.0 32.0 34.0 23.0 34.0 3 31.410130799605135 34.0 33.0 34.0 23.0 34.0 4 31.613104639684106 34.0 34.0 34.0 23.0 34.0 5 31.272889930898323 34.0 33.0 34.0 23.0 34.0 6 34.34553307008885 37.0 35.0 38.0 24.0 38.0 7 33.44133761105627 37.0 34.0 38.0 21.0 38.0 8 32.27129812438302 37.0 31.0 38.0 11.0 38.0 9 33.29537265547878 37.0 34.0 38.0 21.0 38.0 10-11 33.70090078973347 37.0 34.0 38.0 22.0 38.0 12-13 34.144428677196444 38.0 34.5 38.0 23.0 38.0 14-15 33.78658070088845 37.0 34.0 38.0 21.5 38.0 16-17 33.68472976307996 37.0 34.0 38.0 21.0 38.0 18-19 33.64677319842053 37.0 34.0 38.0 22.0 38.0 20-21 33.72165597235933 37.0 34.0 38.0 21.5 38.0 22-23 33.691374629812444 37.0 34.0 38.0 21.5 38.0 24-25 34.15679294175716 37.5 34.5 38.0 23.0 38.0 26-27 34.23074407699902 38.0 34.5 38.0 23.0 38.0 28-29 34.38387833168805 38.0 35.0 38.0 24.0 38.0 30-31 34.21623272458045 38.0 34.5 38.0 23.0 38.0 32-33 33.97337117472853 37.5 34.0 38.0 22.0 38.0 34-35 33.86510982230997 37.0 34.0 38.0 23.0 38.0 36-37 33.84223223099704 37.0 34.0 38.0 22.5 38.0 38-39 34.36504812438302 38.0 35.0 38.0 24.0 38.0 40-41 33.818515547877595 37.5 34.0 38.0 22.0 38.0 42-43 34.18162635735439 37.5 34.0 38.0 23.5 38.0 44-45 33.250728035538 37.0 34.0 38.0 21.0 38.0 46-47 33.93513696939783 37.0 34.0 38.0 23.0 38.0 48-49 33.65355997038499 37.0 34.0 38.0 22.0 38.0 50-51 33.58197186574531 37.0 34.0 38.0 22.0 38.0 52-53 33.61341312931886 37.0 34.0 38.0 22.0 38.0 54-55 33.31594274432379 37.0 33.5 38.0 21.0 38.0 56-57 33.387592546890424 37.0 33.5 38.0 21.5 38.0 58-59 33.27893015794669 37.0 33.0 38.0 22.0 38.0 60-61 33.31299975320829 37.0 33.5 38.0 21.0 38.0 62-63 33.39837117472853 37.0 33.0 38.0 21.5 38.0 64-65 33.422852912142154 37.0 33.5 38.0 21.5 38.0 66-67 33.342787512339584 37.0 33.5 38.0 21.0 38.0 68-69 32.9457058242843 37.0 32.0 38.0 16.0 38.0 70-71 32.83250863770978 37.0 32.0 38.0 19.5 38.0 72-73 32.95850197433366 37.0 32.0 38.0 20.0 38.0 74-75 32.60382527147088 37.0 31.0 38.0 19.0 38.0 76 31.84243583415597 36.0 30.0 38.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.69855526410063 1101 2 -1.1965755215625222 1101 3 -0.014152954017937702 1101 4 -2.488849441613585 1101 5 -0.142335345930114 1101 6 -2.642237081127302 1101 7 -2.2951817391547173 1101 8 -1.6619098808134254 1101 9 -1.495708532300899 1101 10-11 -1.4750737173802122 1101 12-13 -1.2466379581200044 1101 14-15 -0.4305108825981705 1101 16-17 -2.628894789379391 1101 18-19 -0.11557135029810439 1101 20-21 -1.1843625861163218 1101 22-23 -0.4213023917981076 1101 24-25 -1.2429459583956088 1101 26-27 -2.055686388357259 1101 28-29 -0.15867365048472948 1101 30-31 -1.6905691341050257 1101 32-33 0.004264315312440203 1101 34-35 -0.7812412197523884 1101 36-37 0.25388785865663976 1101 38-39 -0.3648806528852191 1101 40-41 0.2642834146389532 1101 42-43 -0.682251373049354 1101 44-45 0.3782918180481616 1101 46-47 0.34355763797653793 1101 48-49 -0.9304864384579403 1101 50-51 -0.6454258688581049 1101 52-53 0.309900959287738 1101 54-55 -1.2304129096956018 1101 56-57 -0.397627098808762 1101 58-59 -0.04582745957655021 1101 60-61 0.5124301505274929 1101 62-63 -2.187791796169499 1101 64-65 -0.6691838465151463 1101 66-67 -1.2832330467801967 1101 68-69 0.23096459400152014 1101 70-71 0.6605197464385384 1101 72-73 -0.9523128227995983 1101 74-75 -1.7675899019381944 1101 76 -1.9107708867357047 1102 1 1.185573738326834 1102 2 -0.019994779451781852 1102 3 -0.3522641550351615 1102 4 0.12282550925914748 1102 5 0.11652490144013328 1102 6 0.7926028217740964 1102 7 1.2743709137177817 1102 8 1.4152372698049973 1102 9 0.6055976750739731 1102 10-11 -0.7488917730963109 1102 12-13 -4.108237773170288 1102 14-15 -1.714212188805547 1102 16-17 -2.2916389808023467 1102 18-19 -0.7369214832307165 1102 20-21 0.03907979048754129 1102 22-23 -0.10818252126291839 1102 24-25 0.345124784871075 1102 26-27 -6.435723378300622 1102 28-29 -0.6637250896247195 1102 30-31 0.08194271423620592 1102 32-33 -7.298429014938407 1102 34-35 -0.7655551718966507 1102 36-37 0.6171406618007822 1102 38-39 0.6332236125176252 1102 40-41 0.7275593364976913 1102 42-43 0.28086271780719585 1102 44-45 -5.310010112364509 1102 46-47 -0.23168221868743188 1102 48-49 0.2561971819327695 1102 50-51 -6.337241843889895 1102 52-53 -1.5864694025201445 1102 54-55 0.7989362984884281 1102 56-57 -1.5531235732047861 1102 58-59 0.6465664833202212 1102 60-61 0.9014487610927517 1102 62-63 -4.816771104919933 1102 64-65 -0.6722933136287779 1102 66-67 -0.47502590309738935 1102 68-69 -7.671855889179621 1102 70-71 -0.977001927356401 1102 72-73 -0.052752078376983036 1102 74-75 -6.987425759064539 1102 76 -2.20141936668022 1103 1 -0.03053592011408668 1103 2 -0.33164701020844234 1103 3 0.035258315956831154 1103 4 0.299018514652186 1103 5 0.272698295785613 1103 6 -1.3862867026580048 1103 7 0.2513279328385494 1103 8 1.8671902032908676 1103 9 0.27235286383030655 1103 10-11 0.72324419683342 1103 12-13 -1.2976682356641618 1103 14-15 -0.7284608405460276 1103 16-17 -0.6408586245349852 1103 18-19 -0.5518728641753157 1103 20-21 -0.3474723422138837 1103 22-23 -0.3380811975172051 1103 24-25 0.7047832257927951 1103 26-27 -0.4332515691814862 1103 28-29 0.6773334983798591 1103 30-31 -0.2336725823808834 1103 32-33 -5.096629292761577 1103 34-35 -1.48320715751521 1103 36-37 -0.2454287939926445 1103 38-39 0.12736072642512397 1103 40-41 0.763768275866866 1103 42-43 0.7023470289691573 1103 44-45 -4.877280091119211 1103 46-47 -0.38571065470639354 1103 48-49 -0.3130032593157992 1103 50-51 -0.11150030115415177 1103 52-53 -0.14851998268030542 1103 54-55 0.599833095350661 1103 56-57 0.8733043561954403 1103 58-59 0.8105434403393446 1103 60-61 0.5722283002331352 1103 62-63 0.010631248405772453 1103 64-65 0.9398573645866151 1103 66-67 0.09561472445614072 1103 68-69 -2.3637683748798963 1103 70-71 0.4629903099372754 1103 72-73 0.9329584135334628 1103 74-75 -1.2233405327203641 1103 76 0.20142423521551578 1104 1 -1.465123281245237 1104 2 -0.11652653697225546 1104 3 0.7294943569321575 1104 4 0.6270195246074586 1104 5 -0.494452908946748 1104 6 -0.1083866882300697 1104 7 1.3868062188001744 1104 8 1.2358960177476561 1104 9 0.3025974172857957 1104 10-11 -0.7612457757535154 1104 12-13 -0.6107363355054503 1104 14-15 -0.4355828287151198 1104 16-17 -0.8949525503754145 1104 18-19 0.6511191283221578 1104 20-21 1.1370987874932226 1104 22-23 0.6144869744637518 1104 24-25 0.3747803402062999 1104 26-27 0.6623154485648755 1104 28-29 -0.8338752016634245 1104 30-31 -0.9918243642305455 1104 32-33 -0.8043283982297638 1104 34-35 -0.11420376695107848 1104 36-37 0.027232077454478087 1104 38-39 -0.3757593139730915 1104 40-41 0.6935312970759355 1104 42-43 1.1694783952943624 1104 44-45 -3.496797476777843 1104 46-47 -0.11165207730053339 1104 48-49 0.5794635564394994 1104 50-51 1.367065201659294 1104 52-53 -0.4596673640105564 1104 54-55 -1.011757944903266 1104 56-57 0.30562833541922174 1104 58-59 0.48780862573827477 1104 60-61 -0.014195015305745073 1104 62-63 0.5553758740009229 1104 64-65 0.13604117644638336 1104 66-67 -0.10605170174498824 1104 68-69 -3.6294497339911374 1104 70-71 -0.7548083482650014 1104 72-73 0.39179371868423374 1104 74-75 -0.847159245748788 1104 76 -0.35924522085143096 1105 1 -1.51085201206811 1105 2 -0.36598609066821197 1105 3 -1.1668968564569617 1105 4 -0.5616136692558626 1105 5 -0.1057499800764532 1105 6 -0.3795338290947825 1105 7 0.37575322019487345 1105 8 -0.09890175073212504 1105 9 0.34189309371536325 1105 10-11 0.574824656603532 1105 12-13 -1.1274814540549656 1105 14-15 -0.263590150890856 1105 16-17 0.2166844789132938 1105 18-19 0.5190323082384793 1105 20-21 0.02961099111944776 1105 22-23 0.21716305535635883 1105 24-25 0.025041846482444896 1105 26-27 -0.2869872013653918 1105 28-29 -0.8467325935741599 1105 30-31 -0.26765246743836 1105 32-33 0.1324655348246324 1105 34-35 0.2120514631744399 1105 36-37 0.026194769225746484 1105 38-39 -0.6044639862185548 1105 40-41 -0.3521799999952009 1105 42-43 0.46017748873926934 1105 44-45 0.668840598535958 1105 46-47 0.15595601196027786 1105 48-49 0.1845542119485657 1105 50-51 -0.5138709908093233 1105 52-53 -1.2395400976228288 1105 54-55 -1.144132421890717 1105 56-57 -0.7013364484022588 1105 58-59 0.20960953229336354 1105 60-61 0.036667955405548014 1105 62-63 -0.17148082055974356 1105 64-65 -0.08270010667774841 1105 66-67 -0.18003182726800304 1105 68-69 0.7442776021315964 1105 70-71 1.0160075513165907 1105 72-73 -0.4177599772711389 1105 74-75 -1.2818175442146185 1105 76 -1.402742431451152 1106 1 -0.803247378286347 1106 2 0.01766488093118923 1106 3 -0.9828721927798334 1106 4 -1.0799245810645317 1106 5 -0.30440468859663383 1106 6 -0.4482744867928403 1106 7 -0.28385066620273136 1106 8 -0.5012186416140381 1106 9 0.6304207469291114 1106 10-11 0.09969386437632721 1106 12-13 -0.06454236586363749 1106 14-15 -0.12370786389534061 1106 16-17 0.11093896321822427 1106 18-19 -0.9846952702368625 1106 20-21 -0.48284229737082995 1106 22-23 -0.4652267054807169 1106 24-25 0.09449251912818823 1106 26-27 0.14358081059275918 1106 28-29 -1.8142680943215481 1106 30-31 -1.0067126318628752 1106 32-33 0.7997581357214898 1106 34-35 0.1646299385107639 1106 36-37 0.20428707116894174 1106 38-39 0.3023839211058075 1106 40-41 -0.5417539910265887 1106 42-43 0.12111732431475275 1106 44-45 0.7585827510023293 1106 46-47 0.3317781345312696 1106 48-49 -0.5283755339408245 1106 50-51 0.12175120650009319 1106 52-53 -1.2501996641400197 1106 54-55 -0.05293287032121441 1106 56-57 0.7417035366500571 1106 58-59 -0.20149846471709765 1106 60-61 0.6839493455400785 1106 62-63 0.5021458610546077 1106 64-65 0.6560704463865221 1106 66-67 1.0983594283373748 1106 68-69 0.8431378412945207 1106 70-71 0.2993035154421477 1106 72-73 0.10114884186189244 1106 74-75 -1.8288280075927936 1106 76 -0.35462957644367776 1107 1 -0.3988664153602919 1107 2 0.14075259245936067 1107 3 0.5914879172055905 1107 4 0.30978550769886937 1107 5 0.20988788000585146 1107 6 0.2566287223455461 1107 7 0.5530165946804715 1107 8 1.328876027045652 1107 9 -0.9084155482599456 1107 10-11 -0.39652308002198566 1107 12-13 0.22640229080100482 1107 14-15 -0.39396566529414656 1107 16-17 0.9061649315881866 1107 18-19 1.2013111148228361 1107 20-21 0.35031572362974117 1107 22-23 -0.49428241789467364 1107 24-25 -0.4101947790319542 1107 26-27 0.48811567929303834 1107 28-29 -0.6295258034507043 1107 30-31 0.2147806601336626 1107 32-33 1.061194958692937 1107 34-35 0.6479568129686797 1107 36-37 -0.038594325424462284 1107 38-39 0.969687248349345 1107 40-41 0.5417500411570657 1107 42-43 0.6430221389450281 1107 44-45 0.4723899812520145 1107 46-47 0.9188160942648054 1107 48-49 0.5337311666810791 1107 50-51 1.0843543178326485 1107 52-53 1.426432124599394 1107 54-55 1.1093140801668966 1107 56-57 1.2637098478940416 1107 58-59 1.252948009659626 1107 60-61 -0.08074459603477635 1107 62-63 0.0706745292344948 1107 64-65 0.8377217040218383 1107 66-67 1.4926276377525767 1107 68-69 1.8758105239422918 1107 70-71 0.6525301850614369 1107 72-73 1.1050795289017046 1107 74-75 0.3451371882956664 1107 76 -0.8767650651960075 1108 1 -1.1622409009569985 1108 2 0.08155664437452259 1108 3 -0.4770464291065366 1108 4 -0.16204101968321183 1108 5 0.18313890881243466 1108 6 0.7351029230419712 1108 7 1.1187019381435945 1108 8 1.0126367108063405 1108 9 -0.5271240003017326 1108 10-11 0.19052978480866045 1108 12-13 0.6909480331245206 1108 14-15 0.6076171140664073 1108 16-17 -0.53155270057389 1108 18-19 0.12976948772566033 1108 20-21 0.6049849227433768 1108 22-23 -0.06162967968637645 1108 24-25 -0.8851077261671279 1108 26-27 -0.9908333552115423 1108 28-29 0.8431712525796797 1108 30-31 0.8286616351257479 1108 32-33 0.28903604764514057 1108 34-35 0.009701035824008386 1108 36-37 0.4064832307642021 1108 38-39 0.9338372958327241 1108 40-41 0.8980336752184783 1108 42-43 0.6893975742093517 1108 44-45 0.7114688036641681 1108 46-47 0.8600158410201075 1108 48-49 0.6580584854528411 1108 50-51 0.7372508040624695 1108 52-53 0.2383979365651996 1108 54-55 0.8102479199896209 1108 56-57 0.9246785088626979 1108 58-59 -0.9295464506126123 1108 60-61 1.5134307759183656 1108 62-63 0.4131879828590641 1108 64-65 0.7107827993051572 1108 66-67 0.3288891129029423 1108 68-69 1.2681023884563842 1108 70-71 2.151263961572994 1108 72-73 0.9130092534980889 1108 74-75 1.5207465583494795 1108 76 1.1202276540189366 1109 1 -0.26205257703986007 1109 2 -0.46139569518798496 1109 3 0.6506878893057504 1109 4 0.011832658427753273 1109 5 0.7603517148387979 1109 6 0.5154520466114292 1109 7 -1.5116761583362006 1109 8 0.532736667346974 1109 9 1.11491569258542 1109 10-11 1.7643020577334738 1109 12-13 0.35560470413712864 1109 14-15 0.2960496796176173 1109 16-17 -0.16138755498501212 1109 18-19 0.632485698068983 1109 20-21 1.1090954543962894 1109 22-23 0.7673396090286744 1109 24-25 0.9714401515671938 1109 26-27 -0.04743917876652404 1109 28-29 0.4871304007761168 1109 30-31 0.3920579845390435 1109 32-33 1.2740968907568373 1109 34-35 0.6616277343608772 1109 36-37 1.0939560686607734 1109 38-39 1.2160515505046021 1109 40-41 0.8012098305132724 1109 42-43 0.9965828559709067 1109 44-45 0.5063405985359637 1109 46-47 0.04649273512411156 1109 48-49 0.62113613285252 1109 50-51 1.3851756193601688 1109 52-53 1.6060460605692626 1109 54-55 0.9492997814991071 1109 56-57 1.051453099620339 1109 58-59 0.6628228091295298 1109 60-61 1.4641043960835134 1109 62-63 -0.15294268496652563 1109 64-65 0.1211840741132093 1109 66-67 0.6556673252743721 1109 68-69 1.7981617829225556 1109 70-71 1.0439736530115056 1109 72-73 0.6815338080395961 1109 74-75 2.1780694614350935 1109 76 0.4471869470799241 1110 1 0.38756752816177453 1110 2 0.27611161048121247 1110 3 -0.16301754611213326 1110 4 0.3593633422383924 1110 5 0.09166381302699378 1110 6 0.708540883548892 1110 7 1.389258967321311 1110 8 1.8973626170839708 1110 9 -1.0293712741007326 1110 10-11 -0.29184201006313515 1110 12-13 -0.1662027184227739 1110 14-15 -0.07759877158050443 1110 16-17 0.587589651327086 1110 18-19 0.7670926530660651 1110 20-21 -0.006955100834566963 1110 22-23 0.11019466455175575 1110 24-25 0.49573150165485913 1110 26-27 -0.7845446726297567 1110 28-29 0.7010403949315815 1110 30-31 0.40641362451565755 1110 32-33 1.3667327762039463 1110 34-35 0.3831721528296157 1110 36-37 0.2200884473866651 1110 38-39 0.679084864356156 1110 40-41 0.5029924137979052 1110 42-43 0.922174615176047 1110 44-45 1.275737150260106 1110 46-47 0.40523762115567763 1110 48-49 0.8895829475807489 1110 50-51 0.12341349194929307 1110 52-53 -0.11619239647340152 1110 54-55 -0.6738738012010685 1110 56-57 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2116 42-43 -0.09200747061032644 2116 44-45 1.814877183901821 2116 46-47 0.14599666236677677 2116 48-49 0.3914647810542604 2116 50-51 0.33993998480043075 2116 52-53 1.1099009592877351 2116 54-55 0.34763587079220315 2116 56-57 -0.40982222075998465 2116 58-59 -0.5141201425033799 2116 60-61 -1.5607405811798145 2116 62-63 0.30245210626952 2116 64-65 0.5064259095824184 2116 66-67 0.31432792882955596 2116 68-69 0.9699889842454184 2116 70-71 -2.9272851316102475 2116 72-73 -0.1035323349947177 2116 74-75 -1.4602728287674651 2116 76 -1.3839416184430213 2117 1 0.3738111084992646 2117 2 0.2099890866367531 2117 3 0.3828371860962285 2117 4 0.25930831655974274 2117 5 -0.1312818949700727 2117 6 -0.5294451766125121 2117 7 -2.43612621242924 2117 8 -2.1049301195264505 2117 9 0.3027086965522443 2117 10-11 0.3371473516389898 2117 12-13 0.8438394679308558 2117 14-15 -0.46319121472064495 2117 16-17 0.1825089469984036 2117 18-19 -0.03376024495300811 2117 20-21 -0.0642276613628141 2117 22-23 0.2966400057109695 2117 24-25 -0.03320283436861615 2117 26-27 0.901487975939574 2117 28-29 0.583010314227252 2117 30-31 0.30943086589497426 2117 32-33 1.2788751092927129 2117 34-35 0.24556210147231639 2117 36-37 -0.5568282804196443 2117 38-39 0.2829916875403029 2117 40-41 -0.5697331914845591 2117 42-43 -1.1106115183529255 2117 44-45 0.4159150666210678 2117 46-47 -0.42028512279149766 2117 48-49 -0.16154507883157265 2117 50-51 1.3067407113183336 2117 52-53 1.0650255052140452 2117 54-55 -0.7151430601885949 2117 56-57 -0.618579356203675 2117 58-59 -0.5805057159335831 2117 60-61 0.7328115724268258 2117 62-63 -0.11629465034697972 2117 64-65 -0.2980369861103753 2117 66-67 -1.5767073591828975 2117 68-69 -0.8497826815563485 2117 70-71 1.5468586151463697 2117 72-73 1.1813534979061657 2117 74-75 -0.08270406903731242 2117 76 -1.4741076796781023 2118 1 0.7266479879318908 2118 2 0.5183889093317866 2118 3 0.7466448102097019 2118 4 0.5946363307441409 2118 5 0.8307083532568811 2118 6 0.9881745042385504 2118 7 1.3799198868615434 2118 8 -3.386401750732123 2118 9 -0.6778985729513067 2118 10-11 -0.5079878433964709 2118 12-13 1.0449143792783673 2118 14-15 -0.7042151508908532 2118 16-17 0.575017812246621 2118 18-19 -0.8361760250948578 2118 20-21 -3.1391390088805515 2118 22-23 -0.16252444464364402 2118 24-25 0.12764601314911062 2118 26-27 1.163012798634604 2118 28-29 0.21576740642584014 2118 30-31 -0.18536080077169714 2118 32-33 0.31788220149129387 2118 34-35 1.2104889631744413 2118 36-37 0.028278102559077922 2118 38-39 0.21949434711477522 2118 40-41 -0.39436749999519805 2118 42-43 -0.2137808445940692 2118 44-45 0.3605072652026351 2118 46-47 -0.21123148803972214 2118 48-49 -1.3810707880514315 2118 50-51 -0.6305376574759904 2118 52-53 -0.4728734309561631 2118 54-55 0.7886800781092802 2118 56-57 0.9934552182644083 2118 58-59 1.032526198960035 2118 60-61 -0.955519544594452 2118 62-63 0.6332066794402564 2118 64-65 -0.11447094001108837 2118 66-67 -1.5644068272680016 2118 68-69 1.007819268798258 2118 70-71 -0.9022216153500828 2118 72-73 -1.5573433106044732 2118 74-75 0.22547412245204868 2118 76 -0.03295076478448422 2119 1 0.3431138513857057 2119 2 0.36860226274544416 2119 3 0.43295254113339965 2119 4 -0.030489171263894832 2119 5 0.1195512247428212 2119 6 0.4548537211060193 2119 7 -1.7297688681585406 2119 8 0.016961703083140378 2119 9 0.022716386888049556 2119 10-11 0.2390314839127683 2119 12-13 0.3312785861056753 2119 14-15 -0.42842448824025325 2119 16-17 -0.4243546777132181 2119 18-19 -0.8388743182675498 2119 20-21 -1.2925024626958148 2119 22-23 0.20252952121981593 2119 24-25 -0.14077140652960196 2119 26-27 0.6067878990362132 2119 28-29 0.435772426506162 2119 30-31 0.08955134782268459 2119 32-33 0.4840468601258294 2119 34-35 0.41596712582504836 2119 36-37 -0.6554568371999565 2119 38-39 0.2170470579581476 2119 40-41 -0.006095662645797972 2119 42-43 -0.3187632743129427 2119 44-45 -0.43341843760861565 2119 46-47 0.37031344167915137 2119 48-49 -0.20179116957753962 2119 50-51 0.8309332260581428 2119 52-53 -0.8610511417995355 2119 54-55 -1.3478974821316854 2119 56-57 0.5316705797101875 2119 58-59 1.4399057170323246 2119 60-61 0.6055685578151895 2119 62-63 0.016395685464352994 2119 64-65 0.05981997364353475 2119 66-67 0.6206458835753708 2119 68-69 -0.7059734018844708 2119 70-71 -0.8433297980810082 2119 72-73 -2.1480812624117007 2119 74-75 0.07581297787373131 2119 76 0.3399784520829847 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 4.0 12 7.0 13 59.0 14 225.0 15 422.0 16 670.0 17 836.0 18 785.0 19 756.0 20 688.0 21 661.0 22 653.0 23 719.0 24 801.0 25 895.0 26 1087.0 27 1347.0 28 1615.0 29 2032.0 30 2480.0 31 3040.0 32 3854.0 33 5119.0 34 7116.0 35 10203.0 36 16625.0 37 18341.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.523661502499195 22.287568696690123 11.901623852982866 31.287145947827817 2 16.731243830207305 20.02714708785785 38.57477788746298 24.666831194471868 3 16.934846989141164 27.01258637709773 29.596495557749257 26.456071076011845 4 12.908440276406713 18.980750246791708 37.6961994076999 30.414610069101677 5 12.243336623889437 36.24629812438302 35.06663376110563 16.443731490621914 6 26.554787759131294 39.51011846001975 17.75542941757157 16.179664363277393 7 26.240128331688055 29.389190523198423 24.618706811451137 19.75197433366239 8 22.406219151036524 34.86426456071076 21.07724580454097 21.652270483711746 9 23.128084896347485 16.377097729516287 23.445212240868706 37.04960513326752 10-11 24.601431391905233 25.438672260612044 27.577739387956562 22.38215695952616 12-13 23.663622902270482 23.885118460019743 26.465325765054292 25.985932872655475 14-15 21.50172754195459 25.209155972359326 24.220138203356367 29.068978282329716 16-17 22.26061204343534 27.54565646594274 26.34192991115499 23.85180157946693 18-19 21.684970384995065 26.527023692003947 26.89844521224087 24.88956071076012 20-21 21.200641658440276 27.372902270483713 26.771964461994074 24.654491609081937 22-23 22.663499506416585 26.932379072063178 27.01443731490622 23.389684106614016 24-25 21.908316880552814 27.759748272458047 26.668928923988155 23.663005923000988 26-27 20.792818361303063 28.026900296150046 27.547507403751233 23.632773938795655 28-29 21.88240375123396 27.049605133267523 28.45508390918065 22.612907206317868 30-31 21.848469891411646 26.99469397828233 28.01085883514314 23.145977295162883 32-33 20.842176702862783 27.947309970384993 27.593780848963473 23.616732477788744 34-35 22.257527147087856 27.634501480750245 27.06071076011846 23.047260612043434 36-37 22.49629812438302 27.0181391905232 27.12364264560711 23.361920039486673 38-39 21.75345508390918 27.71285784797631 27.661648568608094 22.872038499506417 40-41 21.74111549851925 26.782453109575517 27.88993089832182 23.586500493583415 42-43 21.409797630799606 26.68250246791708 27.81959526159921 24.088104639684108 44-45 20.58613030602172 26.400542941757156 28.588351431391906 24.42497532082922 46-47 21.423988153998025 26.15560217176703 28.59267028627838 23.827739387956566 48-49 20.471989141164855 26.36290720631787 29.10291214215202 24.062191510365253 50-51 20.315893385982232 26.254935834155972 28.800592300098714 24.628578479763082 52-53 19.659427443237906 26.415350444225073 29.33613030602172 24.589091806515302 54-55 19.565646594274433 27.027393879565647 28.649432379072064 24.757527147087856 56-57 19.683489634748273 26.81576999012833 28.65375123395854 24.84698914116486 58-59 19.50888450148075 26.311697926949655 28.785784797630797 25.393632773938794 60-61 19.792077986179667 26.416584402764066 28.60500987166831 25.18632773938796 62-63 19.651406712734452 26.34316386969398 28.694471865745314 25.310957551826256 64-65 20.27825765054294 26.25555281342547 27.710389930898323 25.755799605133266 66-67 20.52998519249753 26.27159427443238 27.171767028627837 26.026653504442248 68-69 19.89449654491609 26.423988153998025 27.0897087857848 26.591806515301087 70-71 20.722482724580455 26.28763573543929 26.554170779861796 26.435710760118457 72-73 20.733588351431393 26.744817374136233 26.109328726554786 26.41226554787759 74-75 20.394249753208292 27.38956071076012 25.888450148075027 26.327739387956566 76 20.837857847976306 26.699160908193488 25.920533070088847 26.542448173741363 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 3.0 16 4.5 17 4.0 18 5.5 19 15.0 20 19.5 21 16.0 22 22.0 23 48.0 24 68.0 25 68.0 26 114.5 27 218.5 28 321.0 29 366.0 30 467.0 31 646.0 32 946.0 33 1168.0 34 1384.0 35 1799.5 36 2200.0 37 2401.0 38 2767.0 39 3614.5 40 4430.5 41 4976.0 42 5187.0 43 5442.0 44 5919.0 45 6202.5 46 6264.0 47 6327.0 48 6046.0 49 5470.0 50 5238.0 51 4781.0 52 3792.5 53 2985.5 54 2710.0 55 2568.5 56 2082.5 57 1562.5 58 1387.0 59 1130.5 60 745.5 61 519.5 62 422.0 63 377.5 64 279.0 65 198.5 66 145.0 67 118.0 68 99.5 69 78.5 70 63.0 71 50.0 72 38.5 73 24.5 74 18.5 75 15.0 76 13.0 77 10.5 78 9.5 79 9.0 80 7.0 81 6.5 82 7.0 83 6.0 84 4.0 85 2.5 86 2.5 87 2.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 3.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.7576505429417572 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81040.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82749259624876 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6030443185026 43.53158933859822 2 9.693179597420363 11.016781836130306 3 4.579506221093088 7.807255676209279 4 2.6816928321716285 6.095755182625863 5 1.7175862593099254 4.88030602171767 6 1.1378194688728205 3.879565646594275 7 0.7795366208498904 3.100937808489635 8 0.5667383232362713 2.5765054294175713 9 0.4646819968297396 2.376604146100691 >10 1.7740429505135387 14.658193484698915 >50 0.0021714112001389703 0.07650542941757157 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0037018756169792696 0.0 2 0.0 0.0 0.0 0.004935834155972359 0.0 3 0.0 0.0 0.0 0.004935834155972359 0.0 4 0.0 0.0 0.0 0.006169792694965449 0.0 5 0.0 0.0 0.0 0.006169792694965449 0.0 6 0.0 0.0 0.0 0.006169792694965449 0.0 7 0.0 0.0 0.0 0.007403751233958539 0.0 8 0.0 0.0 0.0 0.009871668311944718 0.0 9 0.0 0.0 0.0 0.027147087857847977 0.0 10 0.0 0.0 0.0 0.041954590325765054 0.0 11 0.0 0.0 0.0 0.044422507403751234 0.0 12 0.0 0.0 0.0 0.046890424481737414 0.0 13 0.0 0.0 0.0 0.051826258637709774 0.0 14 0.0 0.0 0.0 0.056762092793682134 0.0 15 0.0 0.0 0.0 0.06416584402764067 0.0 16 0.0 0.0 0.0 0.06786771964461995 0.0 17 0.0 0.0 0.0 0.0728035538005923 0.0 18 0.0 0.0 0.0 0.07527147087857848 0.0 19 0.0 0.0 0.0 0.07897334649555775 0.0 20 0.0 0.0 0.0 0.08267522211253701 0.0 21 0.0 0.0 0.0 0.0851431391905232 0.0 22 0.0 0.0 0.0 0.09131293188548864 0.0 23 0.0 0.0 0.0 0.0974827245804541 0.0 24 0.0 0.0 0.0 0.10241855873642645 0.0 25 0.0 0.0 0.0 0.10735439289239881 0.0 26 0.0 0.0 0.0 0.109822309970385 0.0 27 0.0 0.0 0.0 0.12956564659427444 0.0 28 0.0 0.0 0.0 0.1468410661401777 0.0 29 0.0 0.0 0.0 0.16288252714708787 0.0 30 0.0 0.0 0.0 0.18015794669299112 0.0 31 0.0 0.0 0.0 0.19743336623889438 0.0 32 0.0 0.0 0.0 0.22581441263573543 0.0 33 0.0 0.0 0.0 0.2578973346495558 0.0 34 0.0 0.0 0.0 0.29861796643632776 0.0 35 0.0 0.0 0.0 0.3763573543928924 0.0 36 0.0 0.0 0.0 0.5207305034550839 0.0 37 0.0 0.0 0.0 0.7144619940769991 0.0 38 0.0 0.0 0.0 1.0093780848963474 0.0 39 0.0 0.0 0.0 1.3228035538005922 0.0 40 0.0 0.0 0.0 1.7497532082922014 0.0 41 0.0 0.0 0.0 2.3840078973346497 0.0 42 0.0 0.0 0.0 3.0306021717670286 0.0 43 0.0 0.0 0.0 3.8129318854886476 0.0 44 0.0 0.0 0.0 4.594027640671273 0.0 45 0.0 0.0 0.0 5.3208292201382035 0.0 46 0.0 0.0 0.0 5.993336623889437 0.0 47 0.0 0.0 0.0 6.792941757156959 0.0 48 0.0 0.0 0.0 7.575271470878579 0.0 49 0.0 0.0 0.0 8.249012833168806 0.0 50 0.0 0.0 0.0 8.937561697926949 0.0 51 0.0 0.0 0.0 9.686574531095756 0.0 52 0.0 0.0 0.0 10.525666337611057 0.0 53 0.0 0.0 0.0 11.38203356367226 0.0 54 0.0 0.0 0.0 12.159427443237908 0.0 55 0.0 0.0 0.0 12.845508390918065 0.0 56 0.0 0.0 0.0 13.515547877591313 0.0 57 0.0 0.0 0.0 14.139930898321817 0.0 58 0.0 0.0 0.0 14.690276406712734 0.0 59 0.0 0.0 0.0 15.308489634748273 0.0 60 0.0 0.0 0.0 15.860069101678183 0.0 61 0.0 0.0 0.0 16.395607107601183 0.0 62 0.0 0.0 0.0 17.113770977295164 0.0 63 0.0 0.0 0.0 17.740621915103652 0.0 64 0.0 0.0 0.0 18.37981243830207 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAAGT 15 0.0022182008 69.99383 16 GCCGAGA 20 7.8877245E-5 69.99383 12 GGCCGAG 30 8.314619E-6 58.32819 11 GATATTC 30 8.314619E-6 58.32819 49 ATATTCG 30 8.314619E-6 58.32819 50 TACGAGT 25 2.3787828E-4 55.995064 4 CGAGTAC 20 0.0069312644 52.49537 6 TCGGGCC 20 0.0069312644 52.49537 41 GGTCAGA 20 0.0069312644 52.49537 49 AGTGGCA 20 0.0069312644 52.49537 12 GGCATTA 20 0.0069312644 52.49537 44 CGACTAC 20 0.0069312644 52.49537 15 ACGAGTA 20 0.0069312644 52.49537 5 TGTGGGA 35 2.0712518E-5 49.995594 25 GAATATG 35 2.0712518E-5 49.995594 54 GGAAGAA 35 2.0712518E-5 49.995594 29 AGGCCGA 35 2.0712518E-5 49.995594 10 ACGCCGA 30 5.84946E-4 46.66255 46 TAAAGTA 30 5.84946E-4 46.66255 15 AAGATAT 30 5.84946E-4 46.66255 47 >>END_MODULE