FastQCFastQC Report
Wed 25 May 2016
SRR1780614_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780614_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81040
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT6010.741609081934847No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT3410.4207798617966436RNA PCR Primer, Index 47 (95% over 21bp)
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC1660.2048371174728529No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTGA252.8172071E-670.0000164
GTCTCGA252.8172071E-670.0000160
TCTCGAG252.8172071E-670.0000161
ACGTCTC252.8172071E-670.0000158
CTCGAGT252.8172071E-670.0000162
TCGAGTG252.8172071E-670.0000163
TCCGCTT150.002217425770.031
AATTTAC301.012304E-770.053
TGAAGGT401.0506483E-861.2528
GCGTGAA401.0506483E-861.2525
GATTCAG401.0506483E-861.2542
CGTCTCG308.310275E-658.33333259
ATGATTC509.622454E-1056.00000440
TTCAGCC452.6619091E-854.44444744
TGGCGTG452.6619091E-854.44444723
ATTGGCG452.6619091E-854.44444721
GAGGCGC452.6619091E-854.44444713
CCCTAGT200.00692884852.51
GAGTAAA200.00692884852.558
TAGCGGG200.00692884852.530
Produced by FastQC (version 0.11.5)