##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780614_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81040 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79118953603159 34.0 34.0 34.0 31.0 34.0 2 33.21733711747285 34.0 34.0 34.0 32.0 34.0 3 33.457379072063176 34.0 34.0 34.0 34.0 34.0 4 33.503319348469894 34.0 34.0 34.0 34.0 34.0 5 33.50336870681145 34.0 34.0 34.0 34.0 34.0 6 37.1958292201382 38.0 37.0 38.0 36.0 38.0 7 36.88373642645607 38.0 37.0 38.0 35.0 38.0 8 36.856256169792694 38.0 37.0 38.0 35.0 38.0 9 36.644114017769006 38.0 37.0 38.0 34.0 38.0 10-11 36.360075271470876 38.0 37.0 38.0 33.5 38.0 12-13 36.2655663869694 38.0 37.0 38.0 34.0 38.0 14-15 36.53622902270483 38.0 37.0 38.0 34.0 38.0 16-17 36.674074531095755 38.0 37.0 38.0 34.0 38.0 18-19 36.66636846001974 38.0 37.0 38.0 34.0 38.0 20-21 36.40458415597236 38.0 37.0 38.0 34.0 38.0 22-23 36.46320952615992 38.0 37.0 38.0 34.0 38.0 24-25 35.83439659427444 38.0 36.5 38.0 32.5 38.0 26-27 36.04157206317868 38.0 37.0 38.0 33.0 38.0 28-29 35.90597852912142 38.0 37.0 38.0 32.5 38.0 30-31 35.612703603158934 38.0 36.0 38.0 31.5 38.0 32-33 36.11955824284304 38.0 37.0 38.0 34.0 38.0 34-35 36.20072186574531 38.0 37.0 38.0 33.5 38.0 36-37 36.33718534057256 38.0 37.0 38.0 34.0 38.0 38-39 35.98052196446199 38.0 36.5 38.0 32.5 38.0 40-41 35.50361549851925 38.0 36.0 38.0 31.0 38.0 42-43 35.87569718657453 38.0 36.5 38.0 32.5 38.0 44-45 36.067880059230006 38.0 37.0 38.0 33.0 38.0 46-47 36.34062808489635 38.0 37.0 38.0 34.0 38.0 48-49 36.14674234945706 38.0 37.0 38.0 34.0 38.0 50-51 36.306928677196446 38.0 37.0 38.0 34.0 38.0 52-53 36.20817497532083 38.0 37.0 38.0 34.0 38.0 54-55 36.39605750246791 38.0 37.0 38.0 34.0 38.0 56-57 36.45913129318855 38.0 37.0 38.0 34.0 38.0 58-59 36.51694225074037 38.0 37.0 38.0 34.0 38.0 60-61 36.217571569595265 38.0 37.0 38.0 33.0 38.0 62-63 36.45881046396841 38.0 37.0 38.0 34.0 38.0 64-65 36.47167448173741 38.0 37.0 38.0 34.0 38.0 66-67 35.88984452122409 38.0 37.0 38.0 32.0 38.0 68-69 35.36358588351432 38.0 36.0 38.0 29.0 38.0 70-71 35.410173988154 38.0 36.0 38.0 31.0 38.0 72-73 35.1591929911155 38.0 36.0 38.0 29.0 38.0 74-75 35.81711500493583 38.0 36.5 38.0 32.0 38.0 76 35.25945212240869 38.0 36.0 38.0 31.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.33144498056999794 1101 2 0.2907960718104974 1101 3 -0.008347508605865528 1101 4 0.21383740081385838 1101 5 0.1558144601682585 1101 6 -1.1376295220626673 1101 7 -0.12737488233830163 1101 8 0.5649637541467172 1101 9 0.2560514165668195 1101 10-11 -0.19803269747338703 1101 12-13 0.7240342743181643 1101 14-15 0.6611837843841002 1101 16-17 -0.21322602833989635 1101 18-19 -0.22129567053866595 1101 20-21 -0.26895231244405693 1101 22-23 0.7963337040202845 1101 24-25 -2.1102340151791026 1101 26-27 -0.5485316494784058 1101 28-29 0.41544383657001305 1101 30-31 0.827421652787848 1101 32-33 -0.6416025534552432 1101 34-35 -0.13524595229015546 1101 36-37 0.12838663606594025 1101 38-39 0.07800204108351494 1101 40-41 0.820497354169639 1101 42-43 0.1877682139759429 1101 44-45 -0.12260033348893984 1101 46-47 0.11897161486609065 1101 48-49 -1.8940455175647344 1101 50-51 -1.9947682514561436 1101 52-53 -2.0303919105642407 1101 54-55 -1.113630489016323 1101 56-57 -1.2225718745382537 1101 58-59 -1.479393446776264 1101 60-61 -1.835979895377342 1101 62-63 -0.8346471988522737 1101 64-65 -1.4337815024916623 1101 66-67 -1.8661728060745872 1101 68-69 -1.3156924847911924 1101 70-71 -1.1362291455094393 1101 72-73 -1.5699131603532592 1101 74-75 -0.8160855861576195 1101 76 -0.6504618799328128 1102 1 -0.6369054991433316 1102 2 0.23064117642268656 1102 3 0.11660914380426135 1102 4 0.3179178539406635 1102 5 -0.009969842224528236 1102 6 0.23566836257494828 1102 7 -0.014370605376100798 1102 8 0.42213633535119754 1102 9 0.9255231539382223 1102 10-11 0.8405986746223206 1102 12-13 0.8337325750926396 1102 14-15 -0.06439707332021527 1102 16-17 0.3798730353521691 1102 18-19 -0.30481202702925003 1102 20-21 0.4681647838453884 1102 22-23 0.6553673417500292 1102 24-25 -4.552841806185924 1102 26-27 -1.0702170037727043 1102 28-29 0.1081152040419795 1102 30-31 1.355083194806113 1102 32-33 0.6138939209407823 1102 34-35 0.730734859176728 1102 36-37 -2.229098047785641 1102 38-39 -0.005560552836264776 1102 40-41 0.7822127627929234 1102 42-43 0.3569278487002592 1102 44-45 0.9426175603961582 1102 46-47 1.3288895434292627 1102 48-49 -2.7544893968850417 1102 50-51 -0.7051485543113074 1102 52-53 -0.21193161695656215 1102 54-55 0.5255904103016817 1102 56-57 0.8621350957541978 1102 58-59 0.22488351542462937 1102 60-61 -5.423264602347636 1102 62-63 -2.4085114919975723 1102 64-65 -0.11311337163973434 1102 66-67 0.7458258068025359 1102 68-69 -0.8029425136895796 1102 70-71 -1.447326128517183 1102 72-73 -6.265543723293959 1102 74-75 -2.313796833412276 1102 76 -0.021864029973272636 1103 1 0.6657174784673927 1103 2 0.6868431702125193 1103 3 -0.0034063816083858 1103 4 0.4199770139341297 1103 5 0.23808948119432927 1103 6 0.2543805704520494 1103 7 0.8609766735910469 1103 8 0.6700730897228269 1103 9 0.8149454451622802 1103 10-11 0.5247280258234994 1103 12-13 0.022131414772331937 1103 14-15 -0.10794994143592618 1103 16-17 0.3165048379342821 1103 18-19 0.8086664825311374 1103 20-21 -0.01725971362151313 1103 22-23 0.41444766533231814 1103 24-25 -2.589786159838482 1103 26-27 -0.22995511457512663 1103 28-29 0.34622600646067525 1103 30-31 1.0365364551427803 1103 32-33 1.1228108191267623 1103 34-35 0.39599879119176506 1103 36-37 -0.795887964438684 1103 38-39 -0.2305871094631584 1103 40-41 0.10777658041018157 1103 42-43 0.12361766729806334 1103 44-45 1.2068151417002966 1103 46-47 1.1612865854295862 1103 48-49 -2.3692242391795304 1103 50-51 -0.19414798232241992 1103 52-53 0.5876169203861394 1103 54-55 0.1048619415976404 1103 56-57 0.4107439370681405 1103 58-59 0.3049211032657837 1103 60-61 -3.4291989870510164 1103 62-63 -1.0184150710137487 1103 64-65 0.29994431752515993 1103 66-67 -0.2429284831141132 1103 68-69 0.5845493150405972 1103 70-71 0.3502510899825708 1103 72-73 -4.7390994513541 1103 74-75 -2.23990812610716 1103 76 -3.482989213826844 1104 1 0.06439732264571774 1104 2 0.24368922417298933 1104 3 0.1886113647485601 1104 4 -0.32962621792274405 1104 5 -0.6582277890408434 1104 6 -0.15573341021451625 1104 7 0.3473062075688276 1104 8 0.3134687036313579 1104 9 0.8298242505294695 1104 10-11 -0.7620902543669459 1104 12-13 -0.1155922181810709 1104 14-15 0.4426921015593166 1104 16-17 0.5091538063371104 1104 18-19 0.5882257091939564 1104 20-21 -0.5352856117603153 1104 22-23 0.15596223754411653 1104 24-25 0.1224938141912375 1104 26-27 0.9113213967508003 1104 28-29 1.3040243039646455 1104 30-31 0.4672012221316635 1104 32-33 0.29698301650088155 1104 34-35 -0.10627360817341014 1104 36-37 0.7716257420971644 1104 38-39 1.1460089805671316 1104 40-41 1.4762479409642069 1104 42-43 0.19721815784777164 1104 44-45 0.6402121984020681 1104 46-47 0.6886726047630205 1104 48-49 0.31737401504063456 1104 50-51 0.3049051846087636 1104 52-53 0.458892710315439 1104 54-55 0.22651646475447507 1104 56-57 0.06661069511171291 1104 58-59 -0.5460022844127579 1104 60-61 0.29011460406137246 1104 62-63 0.22051149080994037 1104 64-65 0.7541356319098114 1104 66-67 -0.25007932714816405 1104 68-69 1.2620399368844843 1104 70-71 1.4308491277837518 1104 72-73 0.8879294766505197 1104 74-75 -0.34020029173982635 1104 76 -1.1319395817391182 1105 1 1.1277864439846326 1105 2 0.3003489173389582 1105 3 0.39846143448356486 1105 4 0.27816666910653964 1105 5 0.021566492688585015 1105 6 0.37751275436009735 1105 7 0.8382755241657591 1105 8 -0.46196714016222984 1105 9 0.8491408474611291 1105 10-11 0.23758722122580167 1105 12-13 -0.24740881511273471 1105 14-15 0.5511736217824676 1105 16-17 0.4711743781641715 1105 18-19 0.9000457928759644 1105 20-21 0.696748907068141 1105 22-23 0.501516630849558 1105 24-25 0.5997253344143871 1105 26-27 0.803450057838667 1105 28-29 0.9077202593342406 1105 30-31 1.0897692137634607 1105 32-33 0.8787734628049222 1105 34-35 -0.8440874157047915 1105 36-37 0.5526752539521169 1105 38-39 0.3706341549046499 1105 40-41 1.1408225574216786 1105 42-43 1.1782153684474963 1105 44-45 1.1042289348037357 1105 46-47 1.0260854360043012 1105 48-49 0.1574280596710409 1105 50-51 0.8381077648040147 1105 52-53 0.8125450813056787 1105 54-55 0.9052211369999412 1105 56-57 0.5771232474129633 1105 58-59 0.6376797239554364 1105 60-61 0.9236745761673717 1105 62-63 0.017791825537976536 1105 64-65 0.7388810991343533 1105 66-67 0.609945080104275 1105 68-69 0.5055981656153108 1105 70-71 0.9776936187182059 1105 72-73 0.6337453762321061 1105 74-75 -0.14601444794623575 1105 76 0.6302291769777568 1106 1 -0.852258399064695 1106 2 0.17833845396077663 1106 3 0.1266191325403696 1106 4 0.17295366462223427 1106 5 0.1381397363358161 1106 6 0.2986824105633872 1106 7 -0.4702820244142103 1106 8 0.40617561021146287 1106 9 -0.9557171495493364 1106 10-11 0.30624379820637415 1106 12-13 1.2349716034223945 1106 14-15 -0.24820044398733643 1106 16-17 0.2528354394496688 1106 18-19 0.6156287525172175 1106 20-21 0.5386491312833783 1106 22-23 0.5979852407150261 1106 24-25 -0.24328662373209653 1106 26-27 0.9280296542512261 1106 28-29 0.8995737720397727 1106 30-31 0.6323981375302807 1106 32-33 0.26525515189310767 1106 34-35 0.7623587726360128 1106 36-37 0.9701453585858957 1106 38-39 0.5829566511677342 1106 40-41 1.1916446799926774 1106 42-43 0.9751137242023447 1106 44-45 0.8741840917544081 1106 46-47 1.0152857349579634 1106 48-49 1.349627237548475 1106 50-51 0.37890559738996643 1106 52-53 0.5036231829499229 1106 54-55 0.2793149337114542 1106 56-57 0.6120447720766435 1106 58-59 0.5152581992917646 1106 60-61 0.9028038736262687 1106 62-63 0.7247424981837085 1106 64-65 -0.4630060458581724 1106 66-67 0.5100385031237025 1106 68-69 1.272068643941175 1106 70-71 0.4874282973430226 1106 72-73 0.23127900851910255 1106 74-75 -0.16833320728854062 1106 76 -0.07242403677411602 1107 1 0.7757288308159076 1107 2 -0.2608650744305905 1107 3 0.17402727810496543 1107 4 0.27317695717238877 1107 5 0.270177603799695 1107 6 0.10318147863993232 1107 7 0.5544277875402486 1107 8 0.3734752466690452 1107 9 0.16951121783149858 1107 10-11 0.6831376327484335 1107 12-13 0.2648854235703979 1107 14-15 0.27043802507464676 1107 16-17 0.4681651189049134 1107 18-19 0.6575560809418022 1107 20-21 0.7435338864920027 1107 22-23 -0.07559242264838417 1107 24-25 0.2856306842086269 1107 26-27 -0.26175055944528225 1107 28-29 0.7713005062478189 1107 30-31 0.7765233289897964 1107 32-33 1.1283362200065667 1107 34-35 0.19495579417175435 1107 36-37 1.0465538547751692 1107 38-39 0.5272442372091746 1107 40-41 0.3033739977508887 1107 42-43 0.8567133108343228 1107 44-45 0.7120478648448909 1107 46-47 0.7994393454693167 1107 48-49 1.0126492530866784 1107 50-51 1.0451293697422912 1107 52-53 0.5391654516760482 1107 54-55 0.4592592028435618 1107 56-57 0.7645203667545317 1107 58-59 0.5726591478237566 1107 60-61 1.190186921846383 1107 62-63 0.8455696033157523 1107 64-65 0.7823481773236622 1107 66-67 0.3841734751660013 1107 68-69 1.1625168898951515 1107 70-71 0.424555758635897 1107 72-73 1.5648153350798424 1107 74-75 -0.20473358374871253 1107 76 0.7638711522863986 1108 1 -0.2692221030239139 1108 2 0.3420155840056225 1108 3 0.18083322935536472 1108 4 0.4808376092774793 1108 5 0.2146220482441379 1108 6 -0.30058553623819506 1108 7 0.16284817373841065 1108 8 -0.40651842221350876 1108 9 -0.26923522091493624 1108 10-11 0.43187140925999046 1108 12-13 1.1309031506992397 1108 14-15 0.561590288449139 1108 16-17 -0.06958416884437923 1108 18-19 0.6023962202263888 1108 20-21 1.1209476250168535 1108 22-23 0.056965348798271975 1108 24-25 0.9187424284314858 1108 26-27 -0.38591960028099237 1108 28-29 -0.18074127912730376 1108 30-31 0.9027499829942371 1108 32-33 1.0527585055399626 1108 34-35 0.8077288236114484 1108 36-37 1.155132518909383 1108 38-39 1.2675892831097855 1108 40-41 1.138685805284915 1108 42-43 -0.18182736659524323 1108 44-45 0.7371349177097244 1108 46-47 0.9818546667735291 1108 48-49 1.214532760525742 1108 50-51 1.0641227220689728 1108 52-53 0.1452908078014019 1108 54-55 0.0686292566580633 1108 56-57 0.4699651277548469 1108 58-59 0.45434639062211346 1108 60-61 1.03467884967165 1108 62-63 0.8968516545978034 1108 64-65 0.9242443469976038 1108 66-67 0.9827014903606894 1108 68-69 1.5984934647606153 1108 70-71 0.11583464435923219 1108 72-73 0.42861717110390174 1108 74-75 -0.1052559009376921 1108 76 1.3740325957811734 1109 1 -0.8620440644899467 1109 2 0.176761346717484 1109 3 0.035678948607859695 1109 4 0.11270056741162904 1109 5 -0.05421034346960596 1109 6 -0.07495899705233455 1109 7 -0.31582052103198066 1109 8 0.4800526338490698 1109 9 0.5336041242972911 1109 10-11 0.15626659975687573 1109 12-13 0.4496957046612806 1109 14-15 0.41046034494630845 1109 16-17 0.5106164685596539 1109 18-19 0.8106390132149457 1109 20-21 0.8182531443562766 1109 22-23 0.5890166308495566 1109 24-25 -0.1936362475065181 1109 26-27 0.12601361716070159 1109 28-29 -0.7792853903832793 1109 30-31 0.567841247661768 1109 32-33 0.49024945150548405 1109 34-35 0.015446482600296463 1109 36-37 0.9713899432176589 1109 38-39 0.9543559063170761 1109 40-41 0.40000334838212126 1109 42-43 0.9458707356791294 1109 44-45 0.9809238500579838 1109 46-47 0.3859724416540189 1109 48-49 0.5680565907444901 1109 50-51 -0.3069840431055937 1109 52-53 -0.2937755401632529 1109 54-55 -0.6724977895537236 1109 56-57 -0.16037675258703388 1109 58-59 0.11606955446391254 1109 60-61 0.7894231637379931 1109 62-63 0.033893520453226245 1109 64-65 0.4362681047840695 1109 66-67 0.22145637953930475 1109 68-69 -0.35178177788751697 1109 70-71 0.29428966391594 1109 72-73 -0.2878647932594163 1109 74-75 -0.2822079507711024 1109 76 0.22500318827719212 1110 1 -0.5584204525670913 1110 2 -0.3217775194426551 1110 3 0.22504189425367827 1110 4 -0.14467815847966392 1110 5 0.30705499843571005 1110 6 0.05179436355550138 1110 7 0.6325283977289828 1110 8 0.747659296619382 1110 9 1.0433937210243514 1110 10-11 -0.5226857672799454 1110 12-13 0.7785969320137056 1110 14-15 1.0256707482192482 1110 16-17 0.6936600103480757 1110 18-19 -0.016764551951624185 1110 20-21 0.8749816656888285 1110 22-23 -0.43900061052975303 1110 24-25 -0.9259930563902046 1110 26-27 0.9252172992179837 1110 28-29 0.3289846271503336 1110 30-31 -0.4462221655468852 1110 32-33 0.640052198437111 1110 34-35 0.9240160325710676 1110 36-37 1.0704913459061416 1110 38-39 0.9849301319161441 1110 40-41 -0.8103268678656832 1110 42-43 0.2680142190222057 1110 44-45 0.5257806589416774 1110 46-47 0.7345624474985541 1110 48-49 1.341982657372192 1110 50-51 -0.34069970646034875 1110 52-53 0.40011692038613944 1110 54-55 -0.4576380584023596 1110 56-57 0.5271232474129661 1110 58-59 -0.7187857932859387 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-0.5006491139767419 2116 46-47 -0.27615033635341746 2116 48-49 0.4547349702401462 2116 50-51 -0.8654999587732206 2116 52-53 0.28180321138697906 2116 54-55 -0.22338658657729837 2116 56-57 -0.27379138673337167 2116 58-59 -1.8354910077518767 2116 60-61 -1.5652481880602664 2116 62-63 0.12388938651358927 2116 64-65 -1.3167083317599477 2116 66-67 -0.566172806074583 2116 68-69 -1.0961802896692419 2116 70-71 0.7125513422954413 2116 72-73 0.008135620134545718 2116 74-75 -0.03803680566981171 2116 76 -0.8065594409084227 2117 1 0.6607651673888881 2117 2 0.5122283499630669 2117 3 0.36645788838427507 2117 4 -0.1295460968509019 2117 5 0.3009104643197915 2117 6 0.22228225790619405 2117 7 0.5416443184920041 2117 8 0.19822789529876417 2117 9 -1.060788230553058 2117 10-11 -0.4698152610436992 2117 12-13 -1.1026393115666338 2117 14-15 -0.027062193820363234 2117 16-17 -1.0553770402755518 2117 18-19 -1.1813371858474397 2117 20-21 -0.23529896527228544 2117 22-23 0.0810379074453067 2117 24-25 -2.1637498428905673 2117 26-27 -2.0070552613102706 2117 28-29 -2.035010237119664 2117 30-31 -0.512757381981217 2117 32-33 -1.5573524237198981 2117 34-35 0.493944499188828 2117 36-37 -1.345640348884757 2117 38-39 -0.06097045502443166 2117 40-41 -0.0710568752024372 2117 42-43 0.10463380816380408 2117 44-45 -0.8308774481749808 2117 46-47 -0.781715982435415 2117 48-49 -0.7850808055771878 2117 50-51 -0.4897734408697474 2117 52-53 0.2663128118021376 2117 54-55 -0.7380856005887111 2117 56-57 0.0577083537959453 2117 58-59 -1.1351926164700927 2117 60-61 -0.5810240053929121 2117 62-63 -0.23805923829181808 2117 64-65 -0.9255692554755726 2117 66-67 -0.7112428631581338 2117 68-69 -0.11377240176057768 2117 70-71 -0.6988134734803779 2117 72-73 1.2268304826150853 2117 74-75 0.596849027231066 2117 76 0.7460093188217272 2118 1 -0.31075522268203315 2118 2 0.4149322506722868 2118 3 -0.7025802321831023 2118 4 0.13441666910654249 2118 5 0.22677482602191645 2118 6 0.042096087693430206 2118 7 0.674733857499092 2118 8 0.43803285983776874 2118 9 -1.7737758192055395 2118 10-11 -0.8759544454408683 2118 12-13 -1.0875129817793976 2118 14-15 0.30377778844913905 2118 16-17 -2.733513121835834 2118 18-19 -0.5541208737907084 2118 20-21 -0.6673135929318619 2118 22-23 0.7244332975162209 2118 24-25 0.6606628344143957 2118 26-27 -2.6132166088280044 2118 28-29 -1.2021755739990923 2118 30-31 -0.9810641195698651 2118 32-33 -0.3519557038617407 2118 34-35 -0.3664832490381187 2118 36-37 -2.549928912714549 2118 38-39 -0.20957417842868153 2118 40-41 -0.5518857759116571 2118 42-43 0.1287362017808249 2118 44-45 -2.60775023186293 2118 46-47 -0.49370623066236163 2118 48-49 -0.21288444032896336 2118 50-51 -0.8879339018626524 2118 52-53 -1.2322465853609827 2118 54-55 -1.0015496963333916 2118 56-57 0.11045658074630182 2118 58-59 -0.5534661093778936 2118 60-61 -1.0877837571659583 2118 62-63 -1.1665831744620263 2118 64-65 -0.1387230675323039 2118 66-67 0.17140341343760923 2118 68-69 -1.9506518343846864 2118 70-71 -0.4072022146151326 2118 72-73 1.0087453762321061 2118 74-75 0.18367305205376283 2118 76 0.746895843644424 2119 1 0.4524852391653553 2119 2 -0.7784663437052188 2119 3 -0.2847212964401251 2119 4 -0.796682728483816 2119 5 -0.4654314992792905 2119 6 -0.5380996954391009 2119 7 -0.8381300983241999 2119 8 -0.06221814417829563 2119 9 -0.6486503171975073 2119 10-11 -0.4027491241557257 2119 12-13 -0.706394758887825 2119 14-15 -0.211502081028776 2119 16-17 -0.44627040095229376 2119 18-19 -0.5508578215818716 2119 20-21 -1.7897721772692137 2119 22-23 -0.41700746553598833 2119 24-25 0.6460104749766415 2119 26-27 -0.0832216289083263 2119 28-29 0.17599335170371688 2119 30-31 0.7238806595465945 2119 32-33 -0.28716529221515685 2119 34-35 -8.64524138528111E-4 2119 36-37 -0.5325155091000937 2119 38-39 -1.9168407446937437 2119 40-41 -0.9716272417751171 2119 42-43 0.35642821985311457 2119 44-45 0.2542289348037414 2119 46-47 -0.03706717443746044 2119 48-49 -1.0304986471562643 2119 50-51 0.15304250375984196 2119 52-53 0.05534126604463552 2119 54-55 -0.08918147344181904 2119 56-57 -1.8290514513822131 2119 58-59 -0.5406335290566062 2119 60-61 0.0734235721513059 2119 62-63 -0.5707623913294952 2119 64-65 -0.6087594631146374 2119 66-67 0.24312781102796777 2119 68-69 0.3666172419205296 2119 70-71 -0.199967023852075 2119 72-73 0.6337453762321061 2119 74-75 -0.22957368489402796 2119 76 0.029526365732778004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 12.0 23 6.0 24 29.0 25 62.0 26 136.0 27 201.0 28 376.0 29 624.0 30 1001.0 31 1710.0 32 2823.0 33 4418.0 34 6851.0 35 10946.0 36 19065.0 37 32777.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.70212240868707 23.511846001974334 12.326011846001975 28.460019743336623 2 17.112537018756168 20.203603158933863 38.230503455083905 24.45335636722606 3 17.219891411648568 25.741609081934847 29.98025666337611 27.058242843040475 4 13.096001974333662 19.239881539980257 38.87092793682132 28.793188548864755 5 12.286525172754196 37.678923988154 36.710266535044425 13.324284304047385 6 27.334649555774927 39.49531095755183 18.579713721618955 14.590325765054294 7 25.86130306021718 30.41214215202369 24.18188548864758 19.54466929911155 8 22.317374136229024 37.21618953603159 20.70705824284304 19.759378084896348 9 23.054047384007898 16.07354392892399 23.919052319842056 36.95335636722606 10-11 24.386105626850938 26.641781836130306 27.383390918065153 21.588721618953603 12-13 23.764807502467917 23.513696939782825 26.829343534057255 25.892152023692006 14-15 21.311697926949655 25.368953603158932 23.684600197433365 29.634748272458044 16-17 22.3389684106614 27.93497038499506 26.050098716683117 23.675962487660414 18-19 22.115621915103652 27.45927936821323 26.728775913129322 23.6963228035538 20-21 21.33576011846002 27.78442744323791 26.441263573543928 24.438548864758143 22-23 21.892275419545904 27.622161895360314 27.590078973346493 22.895483711747286 24-25 22.025542941757156 28.242843040473844 26.78368706811451 22.947926949654494 26-27 21.311080947680157 28.661771964461995 26.95335636722606 23.073790720631788 28-29 21.452986179664364 27.59254689042448 28.53220631786772 22.422260612043434 30-31 21.491855873642645 27.76098223099704 27.947926949654487 22.799234945705823 32-33 22.237783810463966 28.113277393879564 26.906465942744322 22.742472852912144 34-35 22.085389930898323 27.93373642645607 28.134871668311945 21.84600197433366 36-37 22.79800098716683 27.520360315893388 26.823790720631784 22.857847976307998 38-39 21.36105626850938 27.72334649555775 27.89918558736427 23.016411648568607 40-41 21.047630799605134 26.751604146100693 28.698173741362293 23.502591312931887 42-43 21.691757156959525 26.686204343534058 28.62351924975321 22.99851924975321 44-45 20.534921026653503 26.84230009871668 28.722852912142148 23.89992596248766 46-47 21.386352418558737 25.86932379072063 28.944348469891413 23.79997532082922 48-49 20.73050345508391 25.963721618953606 29.15844027640671 24.147334649555773 50-51 19.915473840078974 26.415967423494568 29.204096742349456 24.464461994077 52-53 19.749506416584403 26.11056268509378 30.511475814412638 23.62845508390918 54-55 19.068978282329713 26.99654491609082 29.690276406712734 24.244200394866734 56-57 19.61808983218164 26.49247285291214 29.0893385982231 24.80009871668312 58-59 18.70866238894373 27.122408687068116 29.455207305034552 24.713721618953603 60-61 19.450271470878576 27.386475814412638 28.52171767028628 24.641535044422508 62-63 18.189782823297136 28.247778874629816 28.13548864758144 25.426949654491608 64-65 19.2139684106614 29.02887462981244 26.4819842053307 25.275172754195456 66-67 19.13869693978282 28.221865745310957 26.51283316880553 26.12660414610069 68-69 18.907946692991114 28.451999012833166 25.99765547877591 26.642398815399805 70-71 19.334896347482726 27.986179664363277 26.085266535044422 26.593657453109575 72-73 19.640301085883515 27.79121421520237 25.19002961500494 27.37845508390918 74-75 19.68904244817374 28.558119447186574 25.34797630799605 26.40486179664363 76 19.834649555774924 28.45878578479763 25.222112537018752 26.484452122408687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.5 19 8.5 20 12.5 21 11.0 22 23.0 23 48.5 24 79.0 25 96.0 26 149.0 27 229.5 28 338.0 29 419.0 30 504.5 31 699.5 32 1024.0 33 1239.0 34 1389.5 35 1846.0 36 2276.5 37 2401.0 38 2833.0 39 3907.0 40 4974.0 41 5638.0 42 5877.0 43 6005.0 44 6168.0 45 6314.0 46 6425.0 47 6483.5 48 6101.0 49 5307.5 50 4955.0 51 4498.0 52 3508.0 53 2801.5 54 2628.0 55 2408.0 56 1793.0 57 1235.5 58 1073.0 59 846.0 60 529.0 61 361.5 62 284.0 63 232.5 64 153.5 65 110.5 66 72.5 67 50.0 68 45.5 69 40.0 70 28.0 71 17.0 72 11.0 73 3.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 81040.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.44002961500494 #Duplication Level Percentage of deduplicated Percentage of total 1 62.71327755114194 25.98840078973346 2 14.331655897329007 11.878084896347483 3 7.423398743411845 9.228775913129319 4 4.130065807104785 6.846001974333663 5 2.614418009111753 5.417077986179664 6 1.7836405324122322 4.434846989141165 7 1.4203614924217611 4.120187561697927 8 1.0868594229223119 3.603158933859822 9 0.8337551737486228 3.1095755182625866 >10 3.6327903999047138 23.493336623889437 >50 0.020843879343715573 0.5133267522211253 >100 0.005955394098204448 0.6256169792694966 >500 0.002977697049102224 0.741609081934847 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT 601 0.741609081934847 No Hit ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT 341 0.4207798617966436 RNA PCR Primer, Index 47 (95% over 21bp) ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC 166 0.2048371174728529 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004935834155972359 0.0 2 0.0 0.0 0.0 0.006169792694965449 0.0 3 0.0 0.0 0.0 0.006169792694965449 0.0 4 0.0 0.0 0.0 0.008637709772951628 0.0 5 0.0 0.0 0.0 0.008637709772951628 0.0 6 0.0 0.0 0.0 0.008637709772951628 0.0 7 0.0 0.0 0.0 0.009871668311944718 0.0 8 0.0 0.0 0.0 0.012339585389930898 0.0 9 0.0 0.0 0.0 0.030848963474827247 0.0 10 0.0 0.0 0.0 0.04565646594274432 0.0 11 0.0 0.0 0.0 0.0481243830207305 0.0 12 0.0 0.0 0.0 0.05306021717670286 0.0 13 0.0 0.0 0.0 0.059230009871668314 0.0 14 0.0 0.0 0.0 0.06293188548864759 0.0 15 0.0 0.0 0.0 0.07033563672260612 0.0 16 0.0 0.0 0.0 0.07403751233958539 0.0 17 0.0 0.0 0.0 0.07897334649555775 0.0 18 0.0 0.0 0.0 0.08144126357354393 0.0 19 0.0 0.0 0.0 0.0863770977295163 0.0 20 0.0 0.0 0.0 0.09007897334649556 0.0 21 0.0 0.0 0.0 0.09254689042448173 0.0 22 0.0 0.0 0.0 0.096248766041461 0.0 23 0.0 0.0 0.0 0.10241855873642645 0.0 24 0.0 0.0 0.0 0.10612043435340572 0.0 25 0.0 0.0 0.0 0.11229022704837117 0.0 26 0.0 0.0 0.0 0.11475814412635735 0.0 27 0.0 0.0 0.0 0.1332675222112537 0.0 28 0.0 0.0 0.0 0.15054294175715696 0.0 29 0.0 0.0 0.0 0.16658440276406714 0.0 30 0.0 0.0 0.0 0.18262586377097728 0.0 31 0.0 0.0 0.0 0.20113524185587364 0.0 32 0.0 0.0 0.0 0.2295162882527147 0.0 33 0.0 0.0 0.0 0.26159921026653504 0.0 34 0.0 0.0 0.0 0.30972359328726556 0.0 35 0.0 0.0 0.0 0.39486673247778875 0.0 36 0.0 0.0 0.0 0.5380059230009872 0.0 37 0.0 0.0 0.0 0.7428430404738401 0.0 38 0.0 0.0 0.0 1.0525666337611057 0.0 39 0.0 0.0 0.0 1.388203356367226 0.0 40 0.0 0.0 0.0 1.8435340572556762 0.0 41 0.0 0.0 0.0 2.501233958538993 0.0 42 0.0 0.0 0.0 3.1897828232971372 0.0 43 0.0 0.0 0.0 4.044916090819348 0.0 44 0.0 0.0 0.0 4.867966436327739 0.0 45 0.0 0.0 0.0 5.6428923988154 0.0 46 0.0 0.0 0.0 6.358588351431392 0.0 47 0.0 0.0 0.0 7.191510365251728 0.0 48 0.0 0.0 0.0 8.035538005923001 0.0 49 0.0 0.0 0.0 8.75370187561698 0.0 50 0.0 0.0 0.0 9.486673247778874 0.0 51 0.0 0.0 0.0 10.293682132280356 0.0 52 0.0 0.0 0.0 11.184600197433367 0.0 53 0.0 0.0 0.0 12.074284304047383 0.0 54 0.0 0.0 0.0 12.888696939782823 0.0 55 0.0 0.0 0.0 13.615498519249753 0.0 56 0.0 0.0 0.0 14.318854886475814 0.0 57 0.0 0.0 0.0 14.985192497532083 0.0 58 0.0 0.0 0.0 15.547877591312933 0.0 59 0.0 0.0 0.0 16.208045409674234 0.0 60 0.0 0.0 0.0 16.786771964461995 0.0 61 0.0 0.0 0.0 17.34328726554788 0.0 62 0.0 0.0 0.0 18.09106614017769 0.0 63 0.0 0.0 0.0 18.74629812438302 0.0 64 0.0 0.0 0.0 19.42127344521224 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGTGA 25 2.8172071E-6 70.00001 64 GTCTCGA 25 2.8172071E-6 70.00001 60 TCTCGAG 25 2.8172071E-6 70.00001 61 ACGTCTC 25 2.8172071E-6 70.00001 58 CTCGAGT 25 2.8172071E-6 70.00001 62 TCGAGTG 25 2.8172071E-6 70.00001 63 TCCGCTT 15 0.0022174257 70.0 31 AATTTAC 30 1.012304E-7 70.0 53 TGAAGGT 40 1.0506483E-8 61.25 28 GCGTGAA 40 1.0506483E-8 61.25 25 GATTCAG 40 1.0506483E-8 61.25 42 CGTCTCG 30 8.310275E-6 58.333332 59 ATGATTC 50 9.622454E-10 56.000004 40 TTCAGCC 45 2.6619091E-8 54.444447 44 TGGCGTG 45 2.6619091E-8 54.444447 23 ATTGGCG 45 2.6619091E-8 54.444447 21 GAGGCGC 45 2.6619091E-8 54.444447 13 CCCTAGT 20 0.006928848 52.5 1 GAGTAAA 20 0.006928848 52.5 58 TAGCGGG 20 0.006928848 52.5 30 >>END_MODULE