FastQCFastQC Report
Wed 25 May 2016
SRR1780613_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780613_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036069
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCCTTTAGTGTTGTGTATGGTTATTATTTGTTTTGAGGTTAGTTT11130.10742527766007862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAC250.002365506535.1608843
AGTCGAA454.0556173E-529.34748534
GTCTCGT300.00575417129.323381
TTACGAG508.417426E-526.3642963
TAGCCGG609.776915E-625.6579571
TCGTTGT959.567884E-1025.48597134
CATCGTT959.731593E-1025.44905732
CAAGACT959.786163E-1025.4380044
AAGTCGT701.1154352E-625.1854319
TCGACTC1400.025.12825830
TTTCGAC1400.025.12704528
ATTTCGA1450.024.26059327
TCGAAAT551.5881978E-424.01157836
CCTTACG551.5970739E-423.9918581
TGAGTCG551.6038897E-423.97679732
AAATTCG651.8069843E-523.7025940
TTGTACT1950.023.6591224
ATAACGT752.0491116E-623.48593511
CGTTGAG752.074241E-623.45304130
AATAGCG952.7246642E-823.1254585
Produced by FastQC (version 0.11.5)