FastQCFastQC Report
Wed 25 May 2016
SRR1780613_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780613_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036069
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT143021.3804099919986024No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT49210.47496836600651116TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC27400.264461150753473No Hit
ATAAAGAATATTGAGGCGCCATTGGCGTGAAGGTAGCGGATGATTCAGCC24430.23579510631048703No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG11430.11032083770482469No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGA207.823369E-444.037341
TGCCCGA207.8587944E-443.99696431
CGCTGAT301.3011663E-436.66593
AAGCTTG301.3011663E-436.66595
CCGTCTT6000.035.5676444
GCCGTCT6450.032.74508343
TGTTGCC353.216119E-431.4309510
ATGATTC3250.030.46237440
ATCTAAG300.005743869629.33413741
TTACAGC300.005745224729.332723
ATGCCGT24500.028.9150844
TTCAGCC3350.028.89631544
CTCGCTT852.6375346E-1028.46862218
TATGCCG25100.028.13623243
TATACCG1900.027.7888956
TTGCGAT953.274181E-1127.78755416
ATTGGCG3750.027.5714321
GCGTGAA3700.027.3494625
CGAGAAT1006.002665E-1126.40199714
TGAAGGT3750.026.39817628
Produced by FastQC (version 0.11.5)