##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780612_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6567 Sequences flagged as poor quality 0 Sequence length 76 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.055733211512106 23.0 12.0 32.0 12.0 34.0 2 20.905893101873 21.0 11.0 32.0 11.0 34.0 3 19.88061519719811 21.0 11.0 31.0 11.0 34.0 4 20.825034262220193 21.0 11.0 32.0 11.0 34.0 5 20.740673062281104 21.0 11.0 32.0 11.0 34.0 6 22.785290086797623 23.0 11.0 34.0 11.0 37.0 7 23.45089081772499 26.0 11.0 34.0 11.0 37.0 8 23.612913050098978 26.0 11.0 34.0 11.0 37.0 9 23.518197045835237 24.0 12.0 34.0 12.0 37.0 10-11 22.456753464291154 22.0 11.0 34.0 11.0 37.0 12-13 22.370793360743107 22.0 11.0 34.0 11.0 37.0 14-15 22.002131871478603 21.0 11.0 34.0 11.0 37.0 16-17 21.40231460331963 21.0 11.0 32.0 11.0 37.0 18-19 21.876656007309272 21.5 11.0 33.5 11.0 37.0 20-21 22.079869042180597 22.0 11.0 33.5 11.0 37.0 22-23 21.409700015227653 21.0 11.0 32.5 10.5 37.0 24-25 21.677478300593876 21.0 11.0 32.5 11.0 37.0 26-27 21.39432008527486 21.0 11.0 31.5 11.0 37.0 28-29 20.850768996497642 21.0 11.0 31.0 11.0 37.0 30-31 20.731384193695753 21.0 11.0 31.0 11.0 37.0 32-33 21.018349322369424 21.0 11.0 31.0 11.0 37.0 34-35 21.282016141312624 21.0 11.0 31.5 11.0 37.0 36-37 21.037916857012334 21.0 11.0 31.0 11.0 37.0 38-39 20.48675194152581 16.0 11.0 29.0 11.0 37.0 40-41 20.883356174813464 21.0 11.0 31.0 11.0 37.0 42-43 21.27935130196437 21.0 11.0 32.0 11.0 37.0 44-45 20.656920968478758 21.0 11.0 31.0 11.0 37.0 46-47 20.899954317039743 21.0 11.0 31.0 11.0 37.0 48-49 21.09821836455002 21.0 11.0 31.0 11.0 37.0 50-51 21.320618242728795 21.0 11.0 31.0 11.0 37.0 52-53 21.413126237246843 21.0 11.0 32.0 11.0 37.0 54-55 21.503273945485 21.0 11.0 31.0 11.0 37.0 56-57 20.64169331506015 20.5 11.0 31.0 11.0 37.0 58-59 20.844373382061825 20.0 11.0 31.0 11.0 37.0 60-61 21.008146794578956 21.0 11.0 31.0 11.0 37.0 62-63 20.565478909700015 20.0 11.0 30.0 11.0 36.5 64-65 20.80721790772042 20.0 11.0 30.5 11.0 37.0 66-67 21.178163544997716 20.5 11.0 31.0 11.0 37.0 68-69 20.872392264352065 20.0 11.0 30.5 11.0 36.5 70-71 21.246764123648546 20.5 11.0 31.0 11.0 36.5 72-73 21.055657073245015 20.5 11.0 31.0 11.0 36.5 74-75 20.88655398203137 20.0 11.0 31.0 11.0 36.0 76 19.469316278361504 19.0 11.0 26.0 11.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.23873896763439717 1101 2 -2.4472685113439994 1101 3 -2.0397929061822317 1101 4 -0.4196998208913634 1101 5 -2.860632551437302 1101 6 -1.4296158019058076 1101 7 -3.148506339721507 1101 8 -4.207446292075392 1101 9 -0.7007879854832737 1101 10-11 -2.1011472601683643 1101 12-13 -0.7973550048276117 1101 14-15 -0.9353327560111211 1101 16-17 0.017656743863568636 1101 18-19 -0.79710553509641 1101 20-21 -0.31121418582413085 1101 22-23 -0.3412411902253183 1101 24-25 -1.6025449850807334 1101 26-27 -1.1357167583810295 1101 28-29 -1.7912648046533697 1101 30-31 -1.098640092187427 1101 32-33 -2.5408796826962323 1101 34-35 -1.5473082845562267 1101 36-37 -1.4661817613274977 1101 38-39 -0.8406761795158992 1101 40-41 -0.007385672768283058 1101 42-43 -1.5638286023266517 1101 44-45 -1.5321609405002157 1101 46-47 -0.44557224969325304 1101 48-49 0.07623907429135457 1101 50-51 -2.446316064159163 1101 52-53 0.1205780855869456 1101 54-55 1.2640854174273386 1101 56-57 0.2959735599390534 1101 58-59 -2.7568463159692627 1101 60-61 -0.6053693436901852 1101 62-63 -0.4211981603413655 1101 64-65 -1.6132332052916212 1101 66-67 -0.08495649421234575 1101 68-69 1.1711625151549043 1101 70-71 -1.0347998967519842 1101 72-73 -1.879644741155861 1101 74-75 -2.2468660460716805 1101 76 -0.8795857332336858 1102 1 -1.2354709937781898 1102 2 -1.7413861584028218 1102 3 0.32622016571319534 1102 4 -3.6451900169697957 1102 5 1.7145308472555065 1102 6 0.19456720463013966 1102 7 -3.844584771094059 1102 8 -0.6159430241015365 1102 9 2.318819857653981 1102 10-11 -1.7318662144167298 1102 12-13 -1.875786377376631 1102 14-15 -3.0889275272529524 1102 16-17 0.7545848484387321 1102 18-19 -1.7611578226781077 1102 20-21 -3.216442943994064 1102 22-23 -2.851045111793951 1102 24-25 -1.4554861615513204 1102 26-27 0.040753829854267565 1102 28-29 -2.145839968052062 1102 30-31 -3.976091072579589 1102 32-33 -5.308853538905378 1102 34-35 0.5621688396267785 1102 36-37 -0.619776532569329 1102 38-39 -2.466493172979952 1102 40-41 -4.329281097604888 1102 42-43 -1.8971619356599838 1102 44-45 -0.2609191104348554 1102 46-47 -0.6628925111311652 1102 48-49 -3.984218442048512 1102 50-51 -3.6848781556624317 1102 52-53 0.9490094581359649 1102 54-55 -1.851927654468085 1102 56-57 -4.032457812609966 1102 58-59 0.11897067749479007 1102 60-61 -0.32922555284051214 1102 62-63 -2.2153158074001915 1102 64-65 -1.0184619634615544 1102 66-67 -3.444433618395351 1102 68-69 -4.768379968505229 1102 70-71 -4.7341463019807435 1102 72-73 -3.3257231725284093 1102 74-75 -5.403728791169717 1102 76 -0.9874288704885892 1103 1 3.431195672888478 1103 2 -0.4080528250694897 1103 3 -2.229335389842362 1103 4 -0.42296779474757074 1103 5 -0.007691374966714903 1103 6 -0.1387661287031925 1103 7 1.210970784461498 1103 8 -0.11594302410153645 1103 9 -0.9034023645682439 1103 10-11 -1.4818662144167298 1103 12-13 2.3464358448455904 1103 14-15 1.855516917191494 1103 16-17 -0.2731929293390465 1103 18-19 -0.4833800449003327 1103 20-21 0.811334833783711 1103 22-23 -0.9066006673495011 1103 24-25 0.35006939400423676 1103 26-27 -1.0703572812568467 1103 28-29 -1.090284412496505 1103 30-31 1.162797816309304 1103 32-33 -1.6421868722387138 1103 34-35 1.3677243951823321 1103 36-37 -0.6475543103471075 1103 38-39 -1.022048728535509 1103 40-41 0.004052235728448039 1103 42-43 -0.8416063801044302 1103 44-45 -0.23314133265708037 1103 46-47 -2.0240036222422724 1103 48-49 -1.4564406642707333 1103 50-51 -3.490433711217989 1103 52-53 -1.9121016529751422 1103 54-55 -0.4352609878014171 1103 56-57 -1.504680034832191 1103 58-59 -1.853251544727435 1103 60-61 -1.773669997284955 1103 62-63 -2.3542046962890772 1103 64-65 -0.9906841856837758 1103 66-67 -1.7222113961731296 1103 68-69 -1.7128244129496721 1103 70-71 0.23807592024147795 1103 72-73 0.17427682747159068 1103 74-75 -1.042617680058605 1103 76 -2.7096510927108106 1104 1 -0.17991543822263267 1104 2 1.304068387051725 1104 3 2.1848060242990535 1104 4 1.9760221042423254 1104 5 -0.2804186476939883 1104 6 2.351132861195797 1104 7 3.1402637137544254 1104 8 3.5759761678176574 1104 9 2.6066986455327665 1104 10-11 3.909547926997412 1104 12-13 6.01815301656276 1104 14-15 2.299961361635937 1104 16-17 4.555089898943784 1104 18-19 3.7590441975239095 1104 20-21 2.0992136216625 1104 22-23 3.9141064033575645 1104 24-25 2.1808774748123163 1104 26-27 3.768026557126994 1104 28-29 3.940018617806526 1104 30-31 4.0617877152992 1104 32-33 1.8628636328117913 1104 34-35 2.6050981325560727 1104 36-37 4.723657810865014 1104 38-39 3.2809815744947954 1104 40-41 4.4257694074456175 1104 42-43 3.6407168522188016 1104 44-45 2.827464727948982 1104 46-47 4.483572135333485 1104 48-49 5.98800378017371 1104 50-51 2.145929925145648 1104 52-53 4.2141609732874805 1104 54-55 3.0849410324006037 1104 56-57 3.111481581329425 1104 58-59 1.8487686572927728 1104 60-61 2.774309800694841 1104 62-63 3.4892296471452617 1104 64-65 2.024467329467736 1104 66-67 6.421727997766265 1104 68-69 3.3275796274543694 1104 70-71 1.9451466273121873 1104 72-73 1.3636707668655283 1104 74-75 4.45233181489089 1104 76 1.7246923416326254 1105 1 -1.5890063473135427 1105 2 -3.514113431130095 1105 3 -1.4061030666100365 1105 4 -2.069432441212218 1105 5 -2.0531459204212616 1105 6 -4.60341259334966 1105 7 -4.268827195336481 1105 8 -4.378569286727796 1105 9 -1.30239226355814 1105 10-11 -3.5677248002753146 1105 12-13 -2.73184698343724 1105 14-15 -3.3136750020004264 1105 16-17 -4.467637373783489 1105 18-19 -0.7409558024760869 1105 20-21 -1.8780591056102267 1105 22-23 -1.7904390511878887 1105 24-25 -2.3191225251876837 1105 26-27 -3.936518897418459 1105 28-29 -1.7190722912843874 1105 30-31 -1.574575921064433 1105 32-33 -2.409863639915482 1105 34-35 -1.6221745947166575 1105 36-37 0.01911235631956032 1105 38-39 -1.8553820618688412 1105 40-41 -1.5287760470998357 1105 42-43 -2.768374056872105 1105 44-45 -2.8770807265964713 1105 46-47 -1.198246046484698 1105 48-49 -1.8074507652808371 1105 50-51 -1.1267973475816255 1105 52-53 -1.285839026712516 1105 54-55 -2.4377862403266697 1105 56-57 -1.479427509579665 1105 58-59 -1.2194131608890473 1105 60-61 -2.930235653850616 1105 62-63 -2.192588534672918 1105 64-65 -0.7028053978049904 1105 66-67 -1.328272002233735 1105 68-69 -2.7178749180001773 1105 70-71 -2.9866715545059996 1105 72-73 -2.4999655967708385 1105 74-75 0.52051363307271 1105 76 -0.2298531129128314 1106 1 1.597862339555146 1106 2 -0.296941713958379 1106 3 1.103997943490974 1106 4 0.13258776080798285 1106 5 1.658975291699953 1106 6 -2.083210573147639 1106 7 -0.9001403266496126 1106 8 1.0507236425651314 1106 9 -0.18118014234601887 1106 10-11 -2.3707551033056156 1106 12-13 -1.8202308218210774 1106 14-15 1.1610724727470476 1106 16-17 -3.4120818182279358 1106 18-19 2.1277310662107816 1106 20-21 1.616890389339268 1106 22-23 -0.5177117784606153 1106 24-25 0.10006939400423676 1106 26-27 -1.6536906145901753 1106 28-29 -0.6180621902742836 1106 30-31 0.912797816309304 1106 32-33 0.46892423887239687 1106 34-35 -0.49338671592877503 1106 36-37 -0.17533208812488255 1106 38-39 1.1446179381311588 1106 40-41 -1.6348366531604448 1106 42-43 -1.6193841578822088 1106 44-45 -0.5942524437681875 1106 46-47 0.8371074888688348 1106 48-49 -1.2342184420485118 1106 50-51 -0.4348781556624317 1106 52-53 -1.1343238751973672 1106 54-55 1.9536279010874722 1106 56-57 0.13420885405669836 1106 58-59 0.11897067749479007 1106 60-61 -0.07922555284051214 1106 62-63 0.06246197037758705 1106 64-65 -1.6851286301282222 1106 66-67 0.38889971493798114 1106 68-69 -1.0739355240607864 1106 70-71 -0.31747963531407564 1106 72-73 0.840943494138255 1106 74-75 1.151826764385838 1106 76 -0.0985399815996999 1107 1 -0.7989630572702531 1107 2 -1.7890052060218693 1107 3 -2.022986183493156 1107 4 1.3310004592206823 1107 5 -0.6981675654429047 1107 6 -1.8609883509254175 1107 7 2.036367609858324 1107 8 -1.7508636590221691 1107 9 -0.585942047107924 1107 10-11 2.0221020395515232 1107 12-13 0.6718326702424129 1107 14-15 -0.2714672097926325 1107 16-17 0.996648340502226 1107 18-19 -2.0786181401384276 1107 20-21 2.370858643307521 1107 22-23 1.2918120310631949 1107 24-25 1.2826090765439169 1107 26-27 0.44551573461617267 1107 28-29 3.707334635122539 1107 30-31 -1.5118053582938735 1107 32-33 -0.9358376658895047 1107 34-35 1.2328037602616995 1107 36-37 0.07863616584337052 1107 38-39 -3.5101439666307463 1107 40-41 -0.3015033198271091 1107 42-43 0.6266475881495381 1107 44-45 0.63590628639054 1107 46-47 -0.8811464793851336 1107 48-49 0.2499085420784688 1107 50-51 -1.7404337112179888 1107 52-53 -0.03908577995926876 1107 54-55 -0.41938797192840127 1107 56-57 -2.0205530507052067 1107 58-59 -1.9921404336163206 1107 60-61 -1.5633525369674999 1107 62-63 1.3561127640283779 1107 64-65 -0.38354132854091816 1107 66-67 -0.17459234855408212 1107 68-69 -1.2406021907274507 1107 70-71 2.662679094844652 1107 72-73 -1.3376279344331756 1107 74-75 -0.16563355307447836 1107 76 1.9411425580828414 1108 1 1.2700845617773666 1108 2 0.30861384159717886 1108 3 -0.4515576120645832 1108 4 0.1714766496968707 1108 5 2.542308625033286 1108 6 -2.344321684258748 1108 7 0.9720818955726074 1108 8 0.13961253145401997 1108 9 1.202153190987314 1108 10-11 0.6709115633610487 1108 12-13 1.1658802892900333 1108 14-15 0.7249613616359376 1108 16-17 0.9073626262165106 1108 18-19 -0.4500467115669977 1108 20-21 -0.5053318328829519 1108 22-23 1.4822882215393847 1108 24-25 2.269513838448681 1108 26-27 2.390753829854269 1108 28-29 0.30138225417016073 1108 30-31 4.482242260753747 1108 32-33 1.1105909055390661 1108 34-35 2.22327995073789 1108 36-37 0.9941123563195617 1108 38-39 1.3196179381311595 1108 40-41 0.0734966801728909 1108 42-43 0.1111713976733455 1108 44-45 0.7001920006762568 1108 46-47 1.642663044424392 1108 48-49 0.9380037801737124 1108 50-51 2.7345662887820126 1108 52-53 1.1823427914692992 1108 54-55 0.9508501233096922 1108 56-57 -0.46579114594330306 1108 58-59 1.407859566383678 1108 60-61 -0.3347811083960721 1108 62-63 1.00968419259981 1108 64-65 2.7176491476495563 1108 66-67 1.2444552704935354 1108 68-69 1.8593978092725507 1108 70-71 1.4019648091303658 1108 72-73 0.715943494138255 1108 74-75 0.6796045421636165 1108 76 3.5792377961780772 1109 1 0.1885056144089461 1109 2 -1.4782282636659794 1109 3 1.653705545830153 1109 4 -2.2153654555662854 1109 5 -0.8761124275982937 1109 6 0.2793625262675654 1109 7 2.5326082113620814 1109 8 0.22908621566454812 1109 9 2.494258454145207 1109 10-11 -1.9014568576915813 1109 12-13 -2.4196460264994393 1109 14-15 -3.3842491646798507 1109 16-17 -0.03342684746770175 1109 18-19 3.271005920011948 1109 20-21 -0.4737528855145321 1109 22-23 -0.12297493635535162 1109 24-25 -1.7186440562881629 1109 26-27 -0.69608827540889 1109 28-29 -1.2262493247772106 1109 30-31 -1.0243366866146744 1109 32-33 0.3461172213285373 1109 34-35 0.10749047705367687 1109 36-37 -1.0993086963120184 1109 38-39 -0.1316978513425262 1109 40-41 -0.6436085829850029 1109 42-43 2.4888029766207183 1109 44-45 -1.0445448414290048 1109 46-47 -0.0941790608387656 1109 48-49 0.1722143064894972 1109 50-51 -1.5825389743758826 1109 52-53 -0.02028878747806928 1109 54-55 -0.9294130345850427 1109 56-57 0.6473667487935408 1109 58-59 1.2447016716468369 1109 60-61 -1.3189916347118569 1109 62-63 -0.8995263337159791 1109 64-65 1.3558070423863988 1109 66-67 0.5234026389145932 1109 68-69 -1.0300758749379781 1109 70-71 -3.6230351908696328 1109 72-73 -1.6985301900722725 1109 74-75 -2.4651322999416436 1109 76 -1.7681306248745514 1110 1 0.5200845617773666 1110 2 0.6586138415971767 1110 3 1.8984423879354182 1110 4 1.3214766496968728 1110 5 0.9423086250332844 1110 6 -0.0943216842587482 1110 7 -1.0779181044273898 1110 8 -0.5103874685459786 1110 9 0.5521531909873119 1110 10-11 1.4709115633610494 1110 12-13 0.7658802892900347 1110 14-15 -0.3000386383640645 1110 16-17 -0.9926373737834879 1110 18-19 -2.9750467115669963 1110 20-21 3.2946681671170452 1110 22-23 1.7822882215393818 1110 24-25 -2.305486161551322 1110 26-27 2.2157538298542683 1110 28-29 -0.99861774582984 1110 30-31 -1.2677577392462531 1110 32-33 -1.0144090944609339 1110 34-35 -1.3517200492621093 1110 36-37 2.6441123563195603 1110 38-39 0.7696179381311588 1110 40-41 -0.8515033198271063 1110 42-43 -2.4888286023266524 1110 44-45 -4.099807999323744 1110 46-47 -3.2073369555756095 1110 48-49 -2.3369962198262932 1110 50-51 -2.8904337112179874 1110 52-53 -1.2676572085306965 1110 54-55 1.125850123309693 1110 56-57 -0.8407911459433031 1110 58-59 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2116 76 -5.287428870488588 2117 1 -1.1132487715559662 2117 2 -1.608052825069489 2117 3 -4.31822427873125 2117 4 -2.4118566836364614 2117 5 -1.2410247083000492 2117 6 1.2723449824079154 2117 7 -0.6779181044273912 2117 8 0.5729458647873535 2117 9 -4.881180142346022 2117 10-11 0.5125782300277173 2117 12-13 0.3575469559566997 2117 14-15 0.03329469496927118 2117 16-17 -3.7176373737834894 2117 18-19 -3.2500467115669984 2117 20-21 -3.071998499549622 2117 22-23 -3.2177117784606146 2117 24-25 -1.6221528282179847 2117 26-27 -4.525912836812399 2117 28-29 -2.906951079163175 2117 30-31 -1.2594244059129203 2117 32-33 -1.664409094460936 2117 34-35 -0.310053382595445 2117 36-37 0.4024456896528932 2117 38-39 -0.17204872853551123 2117 40-41 -0.8015033198271091 2117 42-43 -1.230495268993316 2117 44-45 -0.6498079993237447 2117 46-47 -1.0573369555756074 2117 48-49 -0.0119962198262904 2117 50-51 -2.607100377884656 2117 52-53 -2.434323875197368 2117 54-55 -2.4574832100236392 2117 56-57 -3.4157911459433024 2117 58-59 -1.058807100282987 2117 60-61 -0.20144777506273925 2117 62-63 -0.015315807400192227 2117 64-65 -2.007350852350445 2117 66-67 -1.0888780628397932 2117 68-69 -0.473935524060785 2117 70-71 -0.7563685242029656 2117 72-73 -3.6757231725284107 2117 74-75 1.4296045421636165 2117 76 -3.1540955371552535 2118 1 0.5123922540850607 2118 2 -1.3567707737874386 2118 3 3.1638270033200335 2118 4 -1.0939079656877446 2118 5 -0.8153836826590215 2118 6 -4.502013991951056 2118 7 1.3990049724956855 2118 8 -1.868079776238286 2118 9 -1.1170775782434568 2118 10-11 -0.41562689817741116 2118 12-13 1.0216495200592668 2118 14-15 4.45380751548209 2118 16-17 0.5900549339088172 2118 18-19 4.15764559612531 2118 20-21 -3.7207164482675665 2118 22-23 -1.9792502399990752 2118 24-25 -0.03240923847439703 2118 26-27 -0.30540001629957914 2118 28-29 1.8648437926316994 2118 30-31 1.6841653376768235 2118 32-33 -3.2490244790763185 2118 34-35 0.07712610458404257 2118 36-37 0.23065081785802022 2118 38-39 -1.2592282157149945 2118 40-41 1.7754197570959676 2118 42-43 1.3207867822887351 2118 44-45 2.0040381545224086 2118 46-47 -0.9727215709602213 2118 48-49 1.680311472481403 2118 50-51 3.2980278272435513 2118 52-53 -1.2368879777614659 2118 54-55 1.5220039694635403 2118 56-57 1.5765165463643918 2118 58-59 -0.7613712028470871 2118 60-61 -0.5193964930114525 2118 62-63 -0.2537773458617316 2118 64-65 1.1311106861110964 2118 66-67 1.6367629628012317 2118 68-69 -1.894448344573604 2118 70-71 0.7231186552842139 2118 72-73 3.2832511864459484 2118 74-75 1.3911430037020764 2118 76 4.333083950024232 2119 1 -0.17991543822263267 2119 2 4.4728995558828935 2119 3 5.048442387935417 2119 4 5.6643337925540145 2119 5 0.9208800536047121 2119 6 0.5913926014555351 2119 7 0.39351046700118175 2119 8 1.153898245739736 2119 9 1.6521531909873133 2119 10-11 2.9744829919324793 2119 12-13 3.076594575004318 2119 14-15 1.6213899330645063 2119 16-17 1.246648340502226 2119 18-19 2.7428104312901453 2119 20-21 1.9303824528313314 2119 22-23 1.0180025072536694 2119 24-25 5.508799552734395 2119 26-27 3.397896686997125 2119 28-29 3.362096539884444 2119 30-31 2.964385117896601 2119 32-33 0.3141623341104953 2119 34-35 0.3161370935950316 2119 36-37 2.5191123563195603 2119 38-39 2.0017607952740164 2119 40-41 2.3056395373157486 2119 42-43 2.8290285405304907 2119 44-45 2.38590628639054 2119 46-47 2.249805901567246 2119 48-49 2.2737180658879943 2119 50-51 3.366709145924869 2119 52-53 3.10377136289787 2119 54-55 2.1758501233096936 2119 56-57 2.5270659969138407 2119 58-59 2.2578595663836794 2119 60-61 3.3652188916039307 2119 62-63 4.141827049742666 2119 64-65 2.3069348619352716 2119 66-67 2.8015981276363924 2119 68-69 1.9379692378439763 2119 70-71 0.5198219519875131 2119 72-73 2.1623720655668244 2119 74-75 1.3938902564493318 2119 76 -1.8921907752504943 >>END_MODULE >>Per sequence quality scores fail #Quality Count 11 2.0 12 17.0 13 93.0 14 245.0 15 453.0 16 519.0 17 720.0 18 784.0 19 733.0 20 643.0 21 485.0 22 342.0 23 214.0 24 161.0 25 118.0 26 89.0 27 60.0 28 57.0 29 49.0 30 57.0 31 55.0 32 53.0 33 67.0 34 78.0 35 125.0 36 164.0 37 184.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.210026061628085 26.35290510501303 8.845623179518626 54.59144565384025 2 5.253540429419826 22.125780417237703 44.251560834475406 28.369118318867066 3 18.7756966651439 25.963149078726964 26.237246840261914 29.023907415867217 4 5.954012486675803 23.26785442363332 39.48530531445104 31.292827775239836 5 5.466727577280341 28.643216080402013 41.28216841784681 24.607887924470838 6 7.796558550327394 46.62707476777829 14.055124105375361 31.521242576518958 7 7.431094868280798 51.28673671387239 11.61869955839805 29.66346885944876 8 5.603776458047815 38.31277600121821 13.369879701537993 42.71356783919598 9 10.750723313537385 30.790315212425767 12.989188366072787 45.46977310796406 10-11 8.253388152885641 31.871478605146947 23.99878178772651 35.876351454240904 12-13 8.017359524897213 27.128064565250494 24.7144814984011 40.140094411451194 14-15 12.532358763514543 31.37657986904218 19.696969696969695 36.39409167047358 16-17 16.514390132480585 30.143139942134916 19.156388000609105 34.186081924775394 18-19 13.491700928886857 28.04172377036699 17.922948073701843 40.54362722704431 20-21 11.329374143444495 28.673671387239224 19.689355870260393 40.30759859905588 22-23 18.859448758946247 27.95035784985534 16.377341251713112 36.8128521394853 24-25 14.53479518806152 33.637886401705494 15.395157606212884 36.4321608040201 26-27 12.479061976549414 34.33074463225217 20.382214100807065 32.80797929039135 28-29 15.242881072026801 30.211664382518656 23.481041571493833 31.064412973960714 30-31 14.664230242119688 28.795492614588092 25.30074615501751 31.239530988274705 32-33 11.1542561291305 35.1301964367291 22.8643216080402 30.851225826100197 34-35 11.108573168874676 35.60986751941525 20.625856555504797 32.655702756205265 36-37 12.760773564793665 29.80813156692554 22.613065326633166 34.81802954164763 38-39 13.461245622049642 25.719506624029236 27.721943048576215 33.097304705344904 40-41 11.07811786203746 26.724531749657377 25.23983554134308 36.957514846962084 42-43 7.141769453327242 24.851530379168572 27.851378102634385 40.1553220648698 44-45 8.192477539211207 24.470839043703364 31.87909243185625 35.457590985229174 46-47 8.725445408862495 30.584741891274554 28.0721790772042 32.61763362265875 48-49 8.771128369118319 32.36637734125171 22.491244099284298 36.37125019034567 50-51 8.268615806304249 32.214100807065634 20.488807674737323 39.028475711892796 52-53 6.6316430638038675 33.78254910918228 21.50906045378407 38.076747373229786 54-55 6.95142378559464 29.9299527942744 21.33394243947008 41.78468098066088 56-57 11.603471904979443 31.909547738693465 20.176640779655855 36.31033957667123 58-59 10.712654179990864 30.105070808588398 19.171615654027715 40.010659357393024 60-61 7.583371402466881 27.683873915029693 20.846657530074616 43.88609715242881 62-63 11.062890208618851 29.115273336378866 19.18684330744632 40.63499314755596 64-65 10.765950966955993 29.770062433379017 16.36972742500381 43.09425917466119 66-67 7.583371402466881 36.85092127303183 13.811481650677631 41.75422567382366 68-69 10.385259631490786 37.10979138114816 13.407948835084513 39.097000152276536 70-71 7.65950966955992 39.165524592660276 13.21760316735191 39.9573625704279 72-73 7.91837977767626 40.40657834627684 14.527181361352214 37.147860514694685 74-75 9.1061367443277 41.71615654027715 14.1236485457591 35.05405816963606 76 11.177097609258412 40.38373686614893 13.324196741282169 35.11496878331049 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 1.5 15 2.0 16 2.0 17 3.0 18 1.5 19 4.0 20 11.0 21 14.0 22 14.0 23 15.0 24 16.0 25 16.0 26 18.5 27 33.5 28 51.0 29 56.0 30 61.0 31 70.0 32 95.0 33 116.0 34 112.0 35 140.5 36 166.5 37 160.0 38 192.0 39 242.0 40 292.5 41 326.0 42 327.0 43 341.5 44 367.0 45 373.5 46 369.0 47 386.0 48 404.5 49 373.5 50 341.0 51 328.5 52 308.0 53 278.5 54 257.0 55 242.0 56 228.0 57 206.5 58 184.0 59 176.0 60 150.0 61 131.0 62 130.0 63 109.0 64 76.5 65 57.5 66 40.5 67 31.0 68 29.0 69 19.5 70 15.0 71 18.0 72 13.5 73 5.5 74 4.0 75 6.0 76 5.0 77 5.0 78 4.5 79 3.0 80 3.5 81 2.5 82 1.0 83 1.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 1.0 95 1.5 96 1.0 97 1.0 98 0.5 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.6700167504187605 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6567.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.01035480432465 #Duplication Level Percentage of deduplicated Percentage of total 1 97.58921490880253 93.69575148469622 2 1.3639968279143537 2.619156388000609 3 0.6819984139571769 1.9643672910004568 4 0.23790642347343377 0.9136592051164915 5 0.047581284694686754 0.22841480127912286 6 0.0317208564631245 0.1827318410232983 7 0.01586042823156225 0.10659357393025734 8 0.01586042823156225 0.12182122734886554 9 0.0 0.0 >10 0.01586042823156225 0.16750418760469013 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG 11 0.16750418760469013 No Hit TCTTATACACATCTGACGCTGTCTCTTATACACATCTGACGCTGCCGACG 8 0.12182122734886554 No Hit CTATAACAACAACTCCAAAATACAGTGGCTAAAAGCAACCAGCATTTATT 7 0.10659357393025734 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06091061367443277 0.0 2 0.0 0.0 0.0 0.06091061367443277 0.0 3 0.0 0.0 0.0 0.06091061367443277 0.0 4 0.0 0.0 0.0 0.07613826709304096 0.0 5 0.0 0.0 0.0 0.10659357393025735 0.0 6 0.0 0.0 0.0 0.10659357393025735 0.0 7 0.0 0.0 0.0 0.10659357393025735 0.0 8 0.0 0.0 0.0 0.10659357393025735 0.0 9 0.0 0.0 0.0 0.10659357393025735 0.0 10 0.0 0.0 0.0 0.10659357393025735 0.0 11 0.0 0.0 0.0 0.2284148012791229 0.0 12 0.0 0.0 0.0 0.27409776153494747 0.0 13 0.0 0.0 0.0 0.27409776153494747 0.0 14 0.0 0.0 0.0 0.27409776153494747 0.0 15 0.0 0.0 0.0 0.27409776153494747 0.0 16 0.0 0.0 0.0 0.30455306837216384 0.0 17 0.0 0.0 0.0 0.30455306837216384 0.0 18 0.0 0.0 0.0 0.38069133546520484 0.0 19 0.0 0.0 0.0 0.6547890970001523 0.0 20 0.0 0.0 0.0 0.7613826709304097 0.0 21 0.0 0.0 0.0 0.7613826709304097 0.0 22 0.0 0.0 0.0 0.7766103243490178 0.0 23 0.0 0.0 0.0 0.7766103243490178 0.0 24 0.0 0.0 0.0 0.8222932846048424 0.0 25 0.0 0.0 0.0 0.8222932846048424 0.0 26 0.0 0.0 0.0 0.8222932846048424 0.0 27 0.0 0.0 0.0 0.8527485914420588 0.0 28 0.0 0.0 0.0 0.8527485914420588 0.0 29 0.0 0.0 0.0 0.8984315516978834 0.0 30 0.0 0.0 0.0 0.9745698187909243 0.0 31 0.0 0.0 0.0 1.035480432465357 0.0 32 0.0 0.0 0.0 1.187756966651439 0.0 33 0.0 0.0 0.0 1.3095781940003046 0.0 34 0.0 0.0 0.0 1.43139942134917 0.0 35 0.0 0.0 0.0 1.5075376884422111 0.0 36 0.0 0.0 0.0 1.6445865692096848 0.0 37 0.0 0.0 0.0 1.9491396375818486 0.0 38 0.0 0.0 0.0 2.1927820922795798 0.0 39 0.0 0.0 0.0 2.4059692401400943 0.0 40 0.0 0.0 0.0 2.6648393482564336 0.0 41 0.0 0.0 0.0 2.8018882290239073 0.0 42 0.0 0.0 0.0 3.0303030303030303 0.0 43 0.0 0.0 0.0 3.62418151362875 0.0 44 0.0 0.0 0.0 4.233287650373078 0.0 45 0.0 0.0 0.0 4.55306837216385 0.0 46 0.0 0.0 0.0 4.903304400791838 0.0 47 0.0 0.0 0.0 5.22308512258261 0.0 48 0.0 0.0 0.0 5.527638190954774 0.0 49 0.0 0.0 0.0 5.710370031978072 0.0 50 0.0 0.0 0.0 5.801735952489722 0.0 51 0.0 0.0 0.0 6.121516674280493 0.0 52 0.0 0.0 0.0 6.2585655550479675 0.0 53 0.0 0.0 0.0 6.410842089234049 0.0 54 0.0 0.0 0.0 6.776305771280645 0.0 55 0.0 0.0 0.0 6.928582305466728 0.0 56 0.0 0.0 0.0 7.3092736409319325 0.0 57 0.0 0.0 0.0 7.41586721486219 0.0 58 0.0 0.0 0.0 7.689964976397137 0.0 59 0.0 0.0 0.0 7.766103243490178 0.0 60 0.0 0.0 0.0 7.872696817420436 0.0 61 0.0 0.0 0.0 8.055428658443734 0.0 62 0.0 0.0 0.0 8.146794578955383 0.0 63 0.0 0.0 0.0 8.192477539211207 0.0 64 0.0 0.0 0.0 8.283843459722856 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE