FastQCFastQC Report
Wed 25 May 2016
SRR1780612_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780612_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6567
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT243337.04888076747373No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT5888.953860210141617Illumina PCR Primer Index 9 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3254.948987361047663No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1882.86279884269834No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT1291.9643672910004568No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC1051.5989036089538604No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT901.3704888076747372No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT791.2029846200700471No Hit
TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT731.111618699558398Illumina PCR Primer Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC490.7461550175118015No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC370.5634231764885032No Hit
CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT360.5481955230698949No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT270.4111466423024212No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT260.395918988883813No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC220.33500837520938026No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG220.33500837520938026No Hit
CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT210.31978072179077205No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG200.30455306837216384No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT200.30455306837216384No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT180.27409776153494747No Hit
ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT180.27409776153494747Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT170.25887010811633926No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC160.24364245469773108No Hit
CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG160.24364245469773108No Hit
CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT150.22841480127912286No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG150.22841480127912286No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT140.21318714786051468No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT140.21318714786051468No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT130.1979594944419065No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT130.1979594944419065No Hit
ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT120.1827318410232983Illumina PCR Primer Index 9 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC120.1827318410232983No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT120.1827318410232983No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT110.16750418760469013No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATTTCGTATGCCGT100.15227653418608192No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT100.15227653418608192No Hit
CTTATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC100.15227653418608192No Hit
ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT90.13704888076747374Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT90.13704888076747374No Hit
CGTCAGATGTGTATAAGACTGTCTCTTATACACATCTCCGAGCCCACGAG90.13704888076747374No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC90.13704888076747374No Hit
CTTATACCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG90.13704888076747374No Hit
AATAAATGCTGGTTGCTTTTAGCCACTGTATTTTGGAGTTGTTGTTATAG90.13704888076747374No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT80.12182122734886554No Hit
GAAGTGACATGCCTTGAAATAAAATACACACTGAATTTTGTGCAAAATAC80.12182122734886554No Hit
CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT80.12182122734886554No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC80.12182122734886554No Hit
CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT80.12182122734886554No Hit
TCTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC70.10659357393025734No Hit
CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT70.10659357393025734No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG70.10659357393025734No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACCGATCTCGTATGCCGT70.10659357393025734No Hit
CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT70.10659357393025734No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT70.10659357393025734No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTATA150.002127175770.02
CTTTACA207.3557676E-570.01
TCTTTAT150.002127175770.01
TTTACAC207.3557676E-570.02
TTTATAC150.002127175770.03
ACTCGTA307.490922E-658.33333238
AACTCGT307.490922E-658.33333237
AACAGAA307.490922E-658.33333232
ACAGAAC252.2192714E-456.00000433
TTACACA252.2192714E-456.0000043
AGAACTC252.2192714E-456.00000435
GAACTCG252.2192714E-456.00000436
CAGAACT252.2192714E-456.00000434
ATCGTAT351.8670098E-550.039
CTTATAC4850.049.7938161
TTATACA4950.048.080812
TATACAC5100.046.6666683
GCTTGAA4850.046.18556656
CTTGAAA4850.046.18556657
TGCCGTC5200.045.7692345
Produced by FastQC (version 0.11.5)