##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780612_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6567 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92507994518045 34.0 34.0 34.0 32.0 34.0 2 33.26450433988123 34.0 34.0 34.0 32.0 34.0 3 33.49520328917314 34.0 34.0 34.0 34.0 34.0 4 33.5218516826557 34.0 34.0 34.0 34.0 34.0 5 33.548043246535705 34.0 34.0 34.0 34.0 34.0 6 37.32221714633775 38.0 37.0 38.0 37.0 38.0 7 36.808588396528094 38.0 37.0 38.0 34.0 38.0 8 36.89706106289021 38.0 37.0 38.0 35.0 38.0 9 36.71828841175575 38.0 37.0 38.0 34.0 38.0 10-11 36.38685853509974 38.0 37.0 38.0 34.0 38.0 12-13 36.10819247753921 38.0 37.0 38.0 34.0 38.0 14-15 36.30333485609867 38.0 37.0 38.0 34.0 38.0 16-17 36.002740977615346 38.0 36.0 38.0 32.0 38.0 18-19 35.930866453479524 38.0 36.5 38.0 32.0 38.0 20-21 35.74684026191564 38.0 36.0 38.0 32.0 38.0 22-23 36.1335465204812 38.0 36.5 38.0 32.5 38.0 24-25 34.615882442515606 37.0 34.5 38.0 28.0 38.0 26-27 34.82214100807066 37.0 34.0 38.0 29.0 38.0 28-29 35.165067763057706 37.5 35.0 38.0 30.0 38.0 30-31 35.11611085731688 37.0 35.0 38.0 29.0 38.0 32-33 35.56182427287955 37.5 36.0 38.0 31.0 38.0 34-35 34.778056951423785 37.0 35.0 38.0 28.5 38.0 36-37 34.55748439165525 37.0 34.0 38.0 26.5 38.0 38-39 29.92766864626161 34.0 25.5 37.0 11.0 38.0 40-41 32.74234810415715 36.5 32.5 37.0 18.5 38.0 42-43 34.947464595705796 37.0 34.5 38.0 29.0 38.0 44-45 35.44936805238312 37.0 36.0 38.0 31.0 38.0 46-47 35.62737932084666 38.0 36.0 38.0 31.0 38.0 48-49 35.140779655855034 37.5 35.0 38.0 29.0 38.0 50-51 34.958961474036855 37.0 34.5 38.0 29.0 38.0 52-53 34.62410537536166 37.0 34.5 38.0 27.0 38.0 54-55 35.19354347495051 37.0 35.5 38.0 29.0 38.0 56-57 35.144662707476776 37.0 36.0 38.0 27.0 38.0 58-59 34.72064869803563 37.0 34.5 38.0 27.0 38.0 60-61 34.39020861885184 37.0 34.5 38.0 25.5 38.0 62-63 35.20115730165982 37.0 35.5 38.0 29.0 38.0 64-65 35.29808131566926 37.5 36.0 38.0 30.5 38.0 66-67 33.341099436576826 37.0 32.5 38.0 22.0 38.0 68-69 30.55619004111466 35.0 25.5 37.5 11.0 38.0 70-71 26.865159128978224 29.5 17.0 35.5 10.5 38.0 72-73 21.81574539363484 22.0 11.0 30.0 10.5 37.0 74-75 21.167580325871782 21.0 11.0 29.0 11.0 37.0 76 21.5733211512106 21.0 11.0 31.0 11.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.267538661662428 1101 2 0.6481531287136733 1101 3 0.5205167970596918 1101 4 0.5794271252302323 1101 5 0.4216503462409378 1101 6 -1.154675572083299 1101 7 0.9611503892473863 1101 8 1.1321491670298016 1101 9 1.084895520060293 1101 10-11 0.9936291413265081 1101 12-13 1.32154957920703 1101 14-15 1.5176464586166958 1101 16-17 0.09775724435116473 1101 18-19 -0.18951789671501018 1101 20-21 -0.43021344105444115 1101 22-23 0.6914959778263352 1101 24-25 -2.65957783458191 1101 26-27 -0.3623137391865967 1101 28-29 1.581091778324243 1101 30-31 2.373248866139839 1101 32-33 0.9637081182242753 1101 34-35 0.9490635210637848 1101 36-37 1.5684336882316359 1101 38-39 2.042833385654589 1101 40-41 2.7164748026040186 1101 42-43 -0.6200321362911083 1101 44-45 1.844630518283168 1101 46-47 1.1350208007303166 1101 48-49 -1.7798179582240437 1101 50-51 -2.3204124603240572 1101 52-53 -3.589027546670728 1101 54-55 -0.822522331514925 1101 56-57 -1.67663138680431 1101 58-59 -2.334614278141231 1101 60-61 -1.6138467970888115 1101 62-63 -1.8477620068360636 1101 64-65 -1.3393925548316972 1101 66-67 -1.858742748264806 1101 68-69 -0.9357412822476192 1101 70-71 -4.78708477766191 1101 72-73 -3.4894287224507643 1101 74-75 -2.674577357570378 1101 76 -0.4755235555728454 1102 1 -1.2880168938931291 1102 2 0.5435779653149808 1102 3 0.6969873852949888 1102 4 0.5794271252302323 1102 5 0.3105392351298235 1102 6 0.3387884802042862 1102 7 0.52977784022778 1102 8 0.9197308663762129 1102 9 0.8724772194067043 1102 10-11 -0.2727107279545393 1102 12-13 0.9424646118867628 1102 14-15 -0.8810463518408156 1102 16-17 -0.5182558275442588 1102 18-19 0.06701805099740454 1102 20-21 0.9423355785533971 1102 22-23 1.3908423830551015 1102 24-25 -6.15957783458191 1102 26-27 -1.6744052424545686 1102 28-29 0.49939243191901284 1102 30-31 1.1575625916300396 1102 32-33 -0.6523049536711483 1102 34-35 1.2464491419788146 1102 36-37 -3.7191480111147754 1102 38-39 2.196428156896417 1102 40-41 2.255690488878521 1102 42-43 1.3881377983494119 1102 44-45 0.9328658124008129 1102 46-47 1.5860011928871813 1102 48-49 -3.363151291557376 1102 50-51 0.45736531745372133 1102 52-53 0.4028025186887447 1102 54-55 1.1791116554131804 1102 56-57 0.7482052145028817 1102 58-59 -0.4636992454614983 1102 60-61 -7.545219346108418 1102 62-63 -3.4654090656595926 1102 64-65 -1.3116147770539186 1102 66-67 0.8307997353953169 1102 68-69 -2.099139975058076 1102 70-71 -2.5468886992305393 1102 72-73 -3.971454866241615 1102 74-75 -3.7399368346945607 1102 76 -0.25656930720683135 1103 1 0.9897608838846494 1103 2 -0.4564220346850192 1103 3 -0.025234836927232607 1103 4 -0.08723954143643198 1103 5 0.2549836795742735 1103 6 0.39434403575983623 1103 7 0.9742222846722228 1103 8 0.41973086637621293 1103 9 0.6502549971844758 1103 10-11 0.643955938712125 1103 12-13 -1.75197983255768 1103 14-15 -0.7421574629519228 1103 16-17 0.6761886169001841 1103 18-19 0.7059069398862974 1103 20-21 -2.5298866436688243 1103 22-23 1.0852868274995444 1103 24-25 -4.104022279026356 1103 26-27 -1.59107190912124 1103 28-29 0.9993924319190128 1103 30-31 2.4631181471855896 1103 32-33 1.9310283796621803 1103 34-35 0.8575602530899289 1103 36-37 -1.9691480111147754 1103 38-39 1.501983712451974 1103 40-41 0.36680159998963546 1103 42-43 -1.500751090539481 1103 44-45 0.2661991457341486 1103 46-47 1.8915567484427314 1103 48-49 -3.557595736001822 1103 50-51 -1.5426346825462787 1103 52-53 -1.3194197035334696 1103 54-55 -1.9875550112534839 1103 56-57 -0.11290589660823258 1103 58-59 -1.6303659121281626 1103 60-61 -5.517441568330646 1103 62-63 -2.382075732326264 1103 64-65 0.2717185562794171 1103 66-67 -2.8080891534935652 1103 68-69 0.3453044693863667 1103 70-71 0.14755574521391068 1103 72-73 -4.054788199574947 1103 74-75 -1.4899368346945607 1103 76 -3.53434708498461 1104 1 -1.5051886110648454 1104 2 -0.23419981246279775 1104 3 0.515169203476809 1104 4 -2.6478456020424943 1104 5 -0.30562238103178885 1104 6 -0.5450499036341014 1104 7 0.3328081432580774 1104 8 0.08134702799237203 1104 9 0.8068206537501368 1104 10-11 0.2904205851767756 1104 12-13 0.5207474401695862 1104 14-15 0.5911758703814058 1104 16-17 -0.08138714067558084 1104 18-19 1.0872200711994253 1104 20-21 -0.7925129062950873 1104 22-23 0.46154945376216716 1104 24-25 0.8404221654180901 1104 26-27 1.247311929262601 1104 28-29 0.9514126339392206 1104 30-31 -1.0318313477639052 1104 32-33 -0.7154362668024703 1104 34-35 -1.8570862115565347 1104 36-37 1.028326736359979 1104 38-39 4.671175631643891 1104 40-41 3.1723571555451926 1104 42-43 -0.3138824036707959 1104 44-45 -1.5216796421446404 1104 46-47 -1.0655139586279674 1104 48-49 -2.5979997764058638 1104 50-51 0.5432239033123096 1104 52-53 1.195731811618046 1104 54-55 0.456889433190959 1104 56-57 -0.1886634723658034 1104 58-59 -1.953598235360488 1104 60-61 -0.9921890430781133 1104 62-63 -0.8290454292959524 1104 64-65 -1.4757561911953374 1104 66-67 -0.4924325878370013 1104 68-69 1.2594458835277784 1104 70-71 0.6046264522846165 1104 72-73 1.1522825074957623 1104 74-75 1.1034975087397818 1104 76 -1.2616198122573365 1105 1 1.1766295707533345 1105 2 0.7658001875372022 1105 3 0.6969873852949888 1105 4 0.4430634888665992 1105 5 -0.07834965375906222 1105 6 0.1822228236386252 1105 7 0.9237172341671709 1105 8 -0.7368347901894481 1105 9 0.8522751992046835 1105 10-11 0.33587513063131524 1105 12-13 -0.18379801437586707 1105 14-15 0.4775395067450461 1105 16-17 1.145885586597153 1105 18-19 1.0417655257448786 1105 20-21 0.912032548250373 1105 22-23 0.23427672648944053 1105 24-25 1.4995130745089966 1105 26-27 0.6336755656262341 1105 28-29 2.155958088484674 1105 30-31 1.1499868340542747 1105 32-33 1.1482000968338895 1105 34-35 -0.6752680297383549 1105 36-37 -0.8353096272763878 1105 38-39 -0.28336982290156243 1105 40-41 0.8996298828179192 1105 42-43 0.39066305087465736 1105 44-45 0.751047630582633 1105 46-47 0.7526678595538456 1105 48-49 -0.2570906854967703 1105 50-51 1.884132994221396 1105 52-53 -1.0088136429274144 1105 54-55 1.4341621604636856 1105 56-57 0.6295183458160096 1105 58-59 1.5236744919122458 1105 60-61 1.3032655023764335 1105 62-63 1.693681843431314 1105 64-65 1.5696983542592093 1105 66-67 0.2802946848902721 1105 68-69 1.168536792618692 1105 70-71 -0.41810082044265684 1105 72-73 0.3341006893139422 1105 74-75 -0.07832067307839807 1105 76 1.7838347331972066 1106 1 -0.5102391161153506 1106 2 0.043577965314980815 1106 3 -0.6363459480383469 1106 4 -0.08723954143643198 1106 5 -0.7450163204257265 1106 6 0.39434403575983623 1106 7 -1.97022215977222 1106 8 0.6419530885984344 1106 9 -0.6275227805932957 1106 10-11 0.032844827601010707 1106 12-13 1.8313535007756485 1106 14-15 -0.8810463518408156 1106 16-17 1.0095219502335127 1106 18-19 -0.04409306011370262 1106 20-21 0.7478911341089542 1106 22-23 0.6408423830551015 1106 24-25 -3.6040222790263527 1106 26-27 0.1033725353232029 1106 28-29 -0.19505201252543003 1106 30-31 1.2408959249633682 1106 32-33 0.4588061574399518 1106 34-35 1.663115808645486 1106 36-37 2.6697408777741174 1106 38-39 -0.9702385097702475 1106 40-41 1.589023822211864 1106 42-43 -0.4729733127617024 1106 44-45 1.4606435901785915 1106 46-47 1.6971123039982885 1106 48-49 1.4146264862203992 1106 50-51 -0.1815235714351715 1106 52-53 1.5694691853554161 1106 54-55 -0.5986661223645982 1106 56-57 1.164871881169546 1106 58-59 0.7863007545385017 1106 60-61 1.9270028761138036 1106 62-63 0.09014648989596452 1106 64-65 -2.0616147770539257 1106 66-67 0.8585775131731026 1106 68-69 4.817526691608588 1106 70-71 1.7586668563250178 1106 72-73 1.778545133758385 1106 74-75 0.9267298319721071 1106 76 -1.4232359738734992 1107 1 1.219919614043377 1107 2 -0.04372362198660795 1107 3 0.458892147199748 1107 4 0.5794271252302323 1107 5 -0.05454012994953672 1107 6 -0.12946548804968216 1107 7 1.0059683164182545 1107 8 0.9276673743127191 1107 9 -1.2624434155139355 1107 10-11 0.6757019704581566 1107 12-13 -0.09721792779577498 1107 14-15 0.19831872752426705 1107 16-17 -0.03809709738553835 1107 18-19 -0.09568036170099958 1107 20-21 1.632811769029594 1107 22-23 -0.07741158519887392 1107 24-25 -0.6595778345819099 1107 26-27 0.0438487257993998 1107 28-29 -1.0323535998270117 1107 30-31 -0.5567231226556828 1107 32-33 0.2802347288685283 1107 34-35 1.3258142213438973 1107 36-37 1.7491059571392 1107 38-39 1.2361106965789617 1107 40-41 -0.410976177788136 1107 42-43 1.003217163428772 1107 44-45 0.4328658124008129 1107 46-47 0.276477383363364 1107 48-49 -1.398865577271664 1107 50-51 1.4653018253902275 1107 52-53 1.0020088678950998 1107 54-55 0.4449846712861998 1107 56-57 0.8037607700584388 1107 58-59 1.151380119617869 1107 60-61 1.002399701510626 1107 62-63 1.2488766486261227 1107 64-65 0.9463217308825875 1107 66-67 -0.25650185190626473 1107 68-69 3.3373679614498606 1107 70-71 -0.2730791754210138 1107 72-73 0.11981497502822691 1107 74-75 0.24419014943242345 1107 76 -1.5819661326036574 1108 1 -0.3324613383375734 1108 2 0.6658001875372008 1108 3 -0.1530126147050126 1108 4 0.5794271252302323 1108 5 0.12165034624094062 1108 6 -0.3223226309068252 1108 7 -3.403555493105557 1108 8 -1.5413802447349028 1108 9 -0.13863389170440854 1108 10-11 0.524511494267685 1108 12-13 1.6480201674423185 1108 14-15 0.891175870381403 1108 16-17 -0.6904780497664902 1108 18-19 -1.4218708378914826 1108 20-21 1.57566891188673 1108 22-23 -0.6702687280560156 1108 24-25 0.6404221654180873 1108 26-27 -0.27996079801012286 1108 28-29 -2.264496456969873 1108 30-31 -0.45910407503663464 1108 32-33 1.075472824106626 1108 34-35 0.6270046975343746 1108 36-37 2.016963099996339 1108 38-39 0.7075392680075296 1108 40-41 2.6723571555451926 1108 42-43 -0.0979733127617024 1108 44-45 0.7078658124008115 1108 46-47 1.47766785955384 1108 48-49 1.6701820417759592 1108 50-51 -0.29541246032405155 1108 52-53 -0.011086370200146689 1108 54-55 -1.168110566809041 1108 56-57 0.6454274367251003 1108 58-59 0.9918563100940574 1108 60-61 2.021447320558245 1108 62-63 1.1595909343404074 1108 64-65 1.4856074451683057 1108 66-67 1.8780219576175483 1108 68-69 2.323082247164148 1108 70-71 1.061444634102795 1108 72-73 2.334100689313942 1108 74-75 -0.62604794580567 1108 76 1.1156529150153887 1109 1 -0.25877712781125695 1109 2 0.6605370296424624 1109 3 0.6969873852949888 1109 4 0.5794271252302323 1109 5 0.4216503462409378 1109 6 -0.7460068414331431 1109 7 0.4069708226839168 1109 8 0.04546186052825618 1109 9 1.1034713714534874 1109 10-11 1.1205641258466272 1109 12-13 0.15591490428442256 1109 14-15 0.8148600809077209 1109 16-17 0.7463640554966702 1109 18-19 1.5070765305295737 1109 20-21 2.223037332939363 1109 22-23 1.1218365351018775 1109 24-25 -0.4753673082661152 1109 26-27 0.0200392019898743 1109 28-29 -0.810549088548818 1109 30-31 1.1751064512791558 1109 32-33 1.1912622977908285 1109 34-35 -0.09273214457088841 1109 36-37 2.365647310522654 1109 38-39 1.4391182153759523 1109 40-41 -1.9723796865600676 1109 42-43 0.7638687925014551 1109 44-45 1.0381289702955527 1109 46-47 1.4895099648170032 1109 48-49 1.430708357565429 1109 50-51 0.4690612238864702 1109 52-53 -0.9466126859896136 1109 54-55 -1.740478987861671 1109 56-57 1.3388484893566783 1109 58-59 0.736593152199319 1109 60-61 1.910921004768774 1109 62-63 0.4556435659193525 1109 64-65 1.7921863925367276 1109 66-67 1.5267061681438605 1109 68-69 1.6757138261115152 1109 70-71 2.299602528839639 1109 72-73 3.676205952471836 1109 74-75 -0.8510479458056714 1109 76 0.7288108097522326 1110 1 -1.0324613383375727 1110 2 -1.5841998124627992 1110 3 -0.053012614705011174 1110 4 0.27942712523023516 1110 5 0.4216503462409378 1110 6 0.4276773690931748 1110 7 0.2964445068944457 1110 8 1.1586197552651 1110 9 1.1113661082955915 1110 10-11 -0.6254885057323207 1110 12-13 0.24802016744231992 1110 14-15 1.5411758703814087 1110 16-17 1.1345219502335127 1110 18-19 -0.7218708378914798 1110 20-21 1.3756689118867342 1110 22-23 -0.07026872805601414 1110 24-25 -3.8845778345819113 1110 26-27 1.0950392019898771 1110 28-29 -0.18949645696987005 1110 30-31 -1.0341040750366304 1110 32-33 -0.3995271758933754 1110 34-35 1.352004697534369 1110 36-37 1.2169630999963417 1110 38-39 1.5075392680075304 1110 40-41 -1.0276428444548067 1110 42-43 0.7020266872382948 1110 44-45 1.60786581240081 1110 46-47 1.5776678595538485 1110 48-49 2.1451820417759535 1110 50-51 1.529587539675937 1110 52-53 1.013913629799859 1110 54-55 -0.04311056680904102 1110 56-57 0.44542743672509744 1110 58-59 -2.0081436899059426 1110 60-61 -0.7535526794417535 1110 62-63 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-0.2302220325638693 2116 72-73 -2.4992326440193935 2116 74-75 -2.617714612472337 2116 76 -1.8010137516512756 2117 1 1.0008719949957623 2117 2 0.7658001875372022 2117 3 0.6969873852949888 2117 4 0.5794271252302323 2117 5 0.4216503462409378 2117 6 0.4610107024265062 2117 7 0.9297778402277785 2117 8 1.2419530885984287 2117 9 -3.80530055837108 2117 10-11 -0.5671551723989836 2117 12-13 -0.5019798325576801 2117 14-15 1.141175870381403 2117 16-17 -1.323811383099823 2117 18-19 0.29479582877518595 2117 20-21 0.07566891188672997 2117 22-23 -0.22026872805601272 2117 24-25 -0.526244501248577 2117 26-27 -2.013294131343457 2117 28-29 0.2771702096967914 2117 30-31 0.22422925829670248 2117 32-33 -2.491193842560044 2117 34-35 0.20200469753437744 2117 36-37 -0.674703566670324 2117 38-39 1.1408726013408632 2117 40-41 1.2890238222118597 2117 42-43 1.0270266872382976 2117 44-45 -0.9671341875991857 2117 46-47 0.1526678595538442 2117 48-49 0.6868487084426249 2117 50-51 1.54625420634261 2117 52-53 1.3639136297998604 2117 54-55 1.3735560998576304 2117 56-57 0.5370941033917731 2117 58-59 -1.5581436899059398 2117 60-61 0.3881139872249122 2117 62-63 -0.49874239899292405 2117 64-65 -1.8393925548316972 2117 66-67 -0.6469780423824574 2117 68-69 -1.1769177528358554 2117 70-71 -4.396888699230537 2117 72-73 -2.9325659773527235 2117 74-75 2.815618720860993 2117 76 -2.6010137516512764 2118 1 -0.8093844152606486 2118 2 0.5350309567679687 2118 3 -2.533781845474241 2118 4 0.425580971384079 2118 5 0.19088111547170428 2118 6 0.49690813832393843 2118 7 0.6579829684329042 2118 8 1.0778505244958652 2118 9 -2.5078646609351765 2118 10-11 -1.877411582655398 2118 12-13 -0.6942875248653735 2118 14-15 1.2257912549967926 2118 16-17 -5.798170357458794 2118 18-19 0.12043685441621221 2118 20-21 -0.8781772419594205 2118 22-23 0.3951158873286005 2118 24-25 1.76349908849501 2118 26-27 -2.518422336471666 2118 28-29 -0.7356503031237196 2118 30-31 -2.8937194596520186 2118 32-33 -1.0091425605087636 2118 34-35 0.41738931291898496 2118 36-37 -4.838806130772891 2118 38-39 -2.8847684243001623 2118 40-41 -0.346873613685581 2118 42-43 1.4501036103152174 2118 44-45 -1.6825188029838003 2118 46-47 1.2911293980153786 2118 48-49 1.2201820417759563 2118 50-51 -0.5896432295548237 2118 52-53 -1.8899325240462943 2118 54-55 -0.9469567206551943 2118 56-57 1.1627351290327965 2118 58-59 -3.888912920675171 2118 60-61 0.5445242436351663 2118 62-63 -2.8115629118134393 2118 64-65 0.9298382143990764 2118 66-67 -0.023901119305534735 2118 68-69 -3.646148522066621 2118 70-71 -3.7327861351279736 2118 72-73 0.06486992008317216 2118 74-75 0.34125974650202195 2118 76 0.7733452227076967 2119 1 0.1961100902338586 2119 2 0.05151447325148695 2119 3 0.6969873852949888 2119 4 0.4365699823730864 2119 5 -0.07834965375906222 2119 6 -1.772322630906828 2119 7 0.5535873640372984 2119 8 0.45147689812224456 2119 9 0.9042232511527359 2119 10-11 0.7709400656962515 2119 12-13 1.1408773102994658 2119 14-15 0.7340330132385446 2119 16-17 0.830950521662082 2119 18-19 1.4638434478228035 2119 20-21 -0.7600453738275519 2119 22-23 -0.04169729948458212 2119 24-25 2.0547078797038054 2119 26-27 2.2700392019898743 2119 28-29 1.2890749716015577 2119 30-31 2.133753067820514 2119 32-33 0.8040442526780538 2119 34-35 2.6948618403915106 2119 36-37 2.2848202428534776 2119 38-39 -0.6210321605638995 2119 40-41 -3.1847857015976686 2119 42-43 0.7413124015240129 2119 44-45 0.6114372409722435 2119 46-47 0.28838214526813033 2119 48-49 0.47018204177595635 2119 50-51 0.32244468253308156 2119 52-53 0.2281993440855743 2119 54-55 1.2426037189052437 2119 56-57 -1.7438582775606122 2119 58-59 0.8775705958083435 2119 60-61 0.9785901777011077 2119 62-63 0.463162362911838 2119 64-65 1.9463217308825875 2119 66-67 3.1601648147604067 2119 68-69 3.2302251043070065 2119 70-71 1.465016062674227 2119 72-73 2.6555292607425116 2119 74-75 2.5060949113371827 2119 76 -2.962918513556037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 3.0 24 12.0 25 15.0 26 33.0 27 73.0 28 135.0 29 226.0 30 328.0 31 467.0 32 694.0 33 1026.0 34 1402.0 35 1381.0 36 483.0 37 289.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 5.8626465661641545 15.989036089538603 12.654179990863407 65.49413735343383 2 2.619156388000609 4.598751332419674 79.9147251408558 12.867367138723923 3 3.2587178315821532 16.99406121516674 70.50403532815594 9.243185625095172 4 2.619156388000609 57.66712349626923 21.24257651895843 18.47114359677174 5 2.588701081163393 29.206639256890515 63.042485153037916 5.162174508908177 6 5.086036241815136 64.15410385259631 11.329374143444495 19.430485762144052 7 5.0098979747220955 28.856403228262522 8.268615806304249 57.86508299071114 8 3.273945485000761 65.0525354042942 15.943353129282778 15.730165981422262 9 3.989645195675347 14.892645043398812 4.55306837216385 76.56464138876198 10-11 4.5835236790010665 36.645347951880616 38.64017054971829 20.13095781940003 12-13 10.103548043246535 6.250951728338664 38.79244708390437 44.85305314451043 14-15 12.661793817572711 10.590832952641998 10.674585046444344 66.07278818334095 16-17 37.07172224760165 31.924775392112075 3.3120146185472823 27.691487741738996 18-19 37.06410842089234 17.412821684178468 3.1521242576518955 42.370945637277295 20-21 13.24805847418913 6.860057865082991 4.0429419826404756 75.84894167808741 22-23 12.745545911375055 41.38114816506776 3.5175879396984926 42.35571798385869 24-25 36.85092127303183 41.39637581848637 3.5099741129891884 18.242728795492617 26-27 43.83280036546368 38.69346733668342 10.293893710979138 7.179838586873763 28-29 34.27744784528704 17.938175727120452 13.544997715851986 34.23937871174052 30-31 10.925841327851378 13.354652048119384 61.81665905283996 13.90284757118928 32-33 10.271052230851225 68.61580630424852 13.742957210293893 7.370184254606365 34-35 6.540277143292219 49.16247906197655 9.669559920816202 34.627683873915025 36-37 33.94243947007766 38.67062585655551 12.821684178468098 14.565250494898734 38-39 12.791228871630883 13.948530531445105 34.14801279122887 39.11222780569514 40-41 6.52504948987361 10.941068981269986 44.16019491396376 38.37368661489264 42-43 39.470077661032434 6.365159128978225 42.25673823663774 11.908024973351607 44-45 8.70260392873458 33.17344297243795 39.91167961017207 18.2122734886554 46-47 40.22384650525354 6.578346276838739 18.47114359677174 34.726663621135984 48-49 33.637886401705494 4.157149383280037 13.55261154256129 48.652352672453176 50-51 9.760925841327852 3.7916857012334404 45.058626465661646 41.388761991777066 52-53 8.931018730013704 4.492157758489417 72.8490939546216 13.727729556875284 54-55 4.667275772803411 4.568296025582458 49.16247906197655 41.60194913963758 56-57 38.62494289629968 4.865235267245318 20.603015075376884 35.90680676107812 58-59 7.872696817420436 18.37977767626009 61.854728186386474 11.892797319932999 60-61 32.67854423633318 47.723465813918075 14.07035175879397 5.527638190954774 62-63 5.116491548652353 84.91700928886858 4.035328155931171 5.931171006547891 64-65 2.4744936805238313 88.35084513476474 3.3044007918379776 5.870260392873458 66-67 2.9541647632099894 86.66818943200852 3.6622506471752705 6.715395157606213 68-69 2.8323435358611238 81.43749048271661 4.484543931780113 11.245622049642149 70-71 3.746002740977615 73.29069590376123 6.6849398507689965 16.27836150449216 72-73 5.185015989036089 58.62646566164153 10.857316887467642 25.33120146185473 74-75 5.6951423785594635 56.47936652961778 10.918227501142074 26.907263590680675 76 4.065783462768388 60.88015836759555 9.471600426374295 25.582457743261767 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 1.0 19 2.0 20 2.0 21 2.0 22 1.0 23 6.0 24 6.5 25 1.0 26 3.5 27 8.0 28 9.5 29 9.0 30 9.5 31 11.0 32 14.0 33 16.0 34 11.5 35 16.5 36 36.0 37 46.0 38 114.0 39 562.0 40 1231.0 41 1442.0 42 1364.0 43 1162.0 44 709.5 45 357.5 46 256.0 47 191.0 48 101.0 49 57.5 50 39.0 51 32.5 52 28.5 53 29.5 54 28.0 55 41.0 56 35.5 57 28.5 58 40.0 59 43.5 60 49.0 61 57.5 62 64.0 63 49.5 64 36.5 65 30.5 66 16.5 67 10.0 68 8.5 69 6.5 70 4.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6567.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.623267854423634 #Duplication Level Percentage of deduplicated Percentage of total 1 74.85915492957747 16.186995583980508 2 11.901408450704224 5.146946855489569 3 5.422535211267605 3.5175879396984926 4 2.112676056338028 1.827318410232983 5 1.3380281690140845 1.4466270747677783 6 0.5633802816901409 0.7309273640931933 7 0.42253521126760557 0.6395614435815441 8 0.35211267605633806 0.6091061367443277 9 0.42253521126760557 0.8222932846048424 >10 1.971830985915493 8.00974569818791 >50 0.21126760563380279 3.6850921273031827 >100 0.28169014084507044 11.37505710370032 >500 0.07042253521126761 8.953860210141617 >1k 0.07042253521126761 37.04888076747373 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 2433 37.04888076747373 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT 588 8.953860210141617 Illumina PCR Primer Index 9 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 325 4.948987361047663 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 188 2.86279884269834 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT 129 1.9643672910004568 No Hit CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 105 1.5989036089538604 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGT 90 1.3704888076747372 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGT 79 1.2029846200700471 No Hit TATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCT 73 1.111618699558398 Illumina PCR Primer Index 9 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 49 0.7461550175118015 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 37 0.5634231764885032 No Hit CTTATACACATCTCCGAGCCCACGCGACGTTAACAGATCTCGTATGCCGT 36 0.5481955230698949 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGTCTT 27 0.4111466423024212 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCGTCTT 26 0.395918988883813 No Hit TCTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 22 0.33500837520938026 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCCG 22 0.33500837520938026 No Hit CTTATACACATCTCCGAGCCCACGAGCCGTTAACAGATCTCGTATGCCGT 21 0.31978072179077205 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCG 20 0.30455306837216384 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTAGTATGCCGT 20 0.30455306837216384 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGT 18 0.27409776153494747 No Hit ATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCTGCTT 18 0.27409776153494747 Illumina PCR Primer Index 9 (96% over 28bp) CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT 17 0.25887010811633926 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCC 16 0.24364245469773108 No Hit CTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 16 0.24364245469773108 No Hit CTTATACACATCTCCGAGCACACGAGACGTTAACAGATCTCGTATGCCGT 15 0.22841480127912286 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCG 15 0.22841480127912286 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAAATCGTATGCCGT 14 0.21318714786051468 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGAGCTCGTATGCCGT 14 0.21318714786051468 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT 13 0.1979594944419065 No Hit ATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCCGTCTT 13 0.1979594944419065 No Hit ACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTTCT 12 0.1827318410232983 Illumina PCR Primer Index 9 (95% over 24bp) TTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 12 0.1827318410232983 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCCCGTATGCCGT 12 0.1827318410232983 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT 11 0.16750418760469013 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATTTCGTATGCCGT 10 0.15227653418608192 No Hit CTTATACACATCTCCGAGCCCACGAGACGCTAACAGATCTCGTATGCCGT 10 0.15227653418608192 No Hit CTTATCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC 10 0.15227653418608192 No Hit ATACACATCTCCGAGCCCACGAGACGTTACCAGATCTCGTATGCCGTCTT 9 0.13704888076747374 Illumina PCR Primer Index 9 (95% over 22bp) CTTATACACATCTCCGAGCCCACGTGACGTTAACAGATCTCGTATGCCGT 9 0.13704888076747374 No Hit CGTCAGATGTGTATAAGACTGTCTCTTATACACATCTCCGAGCCCACGAG 9 0.13704888076747374 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGACCTCGTATGCC 9 0.13704888076747374 No Hit CTTATACCACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 9 0.13704888076747374 No Hit AATAAATGCTGGTTGCTTTTAGCCACTGTATTTTGGAGTTGTTGTTATAG 9 0.13704888076747374 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCACAGATCTCGTATGCCGT 8 0.12182122734886554 No Hit GAAGTGACATGCCTTGAAATAAAATACACACTGAATTTTGTGCAAAATAC 8 0.12182122734886554 No Hit CTTATACACATCTCCGAGCCCACGAGAAGTTAACAGATCTCGTATGCCGT 8 0.12182122734886554 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGAACTCGTATGCC 8 0.12182122734886554 No Hit CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT 8 0.12182122734886554 No Hit TCTTATTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC 7 0.10659357393025734 No Hit CTTATACACATATCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 7 0.10659357393025734 No Hit CTTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG 7 0.10659357393025734 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACCGATCTCGTATGCCGT 7 0.10659357393025734 No Hit CTTATACACATCTACGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT 7 0.10659357393025734 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT 7 0.10659357393025734 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06091061367443277 0.0 2 0.0 0.0 0.0 0.06091061367443277 0.0 3 0.0 0.0 0.0 0.06091061367443277 0.0 4 0.0 0.0 0.0 0.09136592051164916 0.0 5 0.0 0.0 0.0 0.12182122734886554 0.0 6 0.0 0.0 0.0 0.12182122734886554 0.0 7 0.0 0.0 0.0 0.12182122734886554 0.0 8 0.0 0.0 0.0 0.12182122734886554 0.0 9 0.0 0.0 0.0 0.13704888076747374 0.0 10 0.0 0.0 0.0 0.13704888076747374 0.0 11 0.0 0.0 0.0 0.24364245469773108 0.0 12 0.0 0.0 0.0 0.25887010811633926 0.0 13 0.0 0.0 0.0 0.25887010811633926 0.0 14 0.0 0.0 0.0 0.27409776153494747 0.0 15 0.0 0.0 0.0 0.27409776153494747 0.0 16 0.0 0.0 0.0 0.30455306837216384 0.0 17 0.0 0.0 0.0 0.30455306837216384 0.0 18 0.0 0.0 0.0 0.395918988883813 0.0 19 0.0 0.0 0.0 0.6243337901629359 0.0 20 0.0 0.0 0.0 0.7613826709304097 0.0 21 0.0 0.0 0.0 0.7613826709304097 0.0 22 0.0 0.0 0.0 0.7766103243490178 0.0 23 0.0 0.0 0.0 0.7766103243490178 0.0 24 0.0 0.0 0.0 0.7766103243490178 0.0 25 0.0 0.0 0.0 0.7766103243490178 0.0 26 0.0 0.0 0.0 0.7766103243490178 0.0 27 0.0 0.0 0.0 0.8070656311862342 0.0 28 0.0 0.0 0.0 0.8222932846048424 0.0 29 0.0 0.0 0.0 0.8527485914420588 0.0 30 0.0 0.0 0.0 0.9288868585350998 0.0 31 0.0 0.0 0.0 1.0050251256281406 0.0 32 0.0 0.0 0.0 1.1573016598142227 0.0 33 0.0 0.0 0.0 1.2486675803258718 0.0 34 0.0 0.0 0.0 1.3704888076747372 0.0 35 0.0 0.0 0.0 1.43139942134917 0.0 36 0.0 0.0 0.0 1.583675955535252 0.0 37 0.0 0.0 0.0 1.9948225978376732 0.0 38 0.0 0.0 0.0 2.238465052535404 0.0 39 0.0 0.0 0.0 2.451652200395919 0.0 40 0.0 0.0 0.0 2.7257499619308665 0.0 41 0.0 0.0 0.0 2.8323435358611238 0.0 42 0.0 0.0 0.0 3.0607583371402467 0.0 43 0.0 0.0 0.0 3.548043246535709 0.0 44 0.0 0.0 0.0 4.233287650373078 0.0 45 0.0 0.0 0.0 4.537840718745241 0.0 46 0.0 0.0 0.0 4.88807674737323 0.0 47 0.0 0.0 0.0 5.22308512258261 0.0 48 0.0 0.0 0.0 5.512410537536166 0.0 49 0.0 0.0 0.0 5.740825338815289 0.0 50 0.0 0.0 0.0 5.816963605908329 0.0 51 0.0 0.0 0.0 6.151971981117709 0.0 52 0.0 0.0 0.0 6.304248515303792 0.0 53 0.0 0.0 0.0 6.48698035632709 0.0 54 0.0 0.0 0.0 6.837216384955078 0.0 55 0.0 0.0 0.0 6.974265265722552 0.0 56 0.0 0.0 0.0 7.385411908024973 0.0 57 0.0 0.0 0.0 7.492005481955231 0.0 58 0.0 0.0 0.0 7.720420283234353 0.0 59 0.0 0.0 0.0 7.872696817420436 0.0 60 0.0 0.0 0.0 7.9640627379320845 0.0 61 0.0 0.0 0.0 8.131566925536776 0.0 62 0.0 0.0 0.0 8.222932846048424 0.0 63 0.0 0.0 0.0 8.253388152885641 0.0 64 0.0 0.0 0.0 8.359981726815898 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTATA 15 0.0021271757 70.0 2 CTTTACA 20 7.3557676E-5 70.0 1 TCTTTAT 15 0.0021271757 70.0 1 TTTACAC 20 7.3557676E-5 70.0 2 TTTATAC 15 0.0021271757 70.0 3 ACTCGTA 30 7.490922E-6 58.333332 38 AACTCGT 30 7.490922E-6 58.333332 37 AACAGAA 30 7.490922E-6 58.333332 32 ACAGAAC 25 2.2192714E-4 56.000004 33 TTACACA 25 2.2192714E-4 56.000004 3 AGAACTC 25 2.2192714E-4 56.000004 35 GAACTCG 25 2.2192714E-4 56.000004 36 CAGAACT 25 2.2192714E-4 56.000004 34 ATCGTAT 35 1.8670098E-5 50.0 39 CTTATAC 485 0.0 49.793816 1 TTATACA 495 0.0 48.08081 2 TATACAC 510 0.0 46.666668 3 GCTTGAA 485 0.0 46.185566 56 CTTGAAA 485 0.0 46.185566 57 TGCCGTC 520 0.0 45.76923 45 >>END_MODULE