FastQCFastQC Report
Wed 25 May 2016
SRR1780611_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780611_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56070
Sequences flagged as poor quality0
Sequence length50
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG600.1070090957731407No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA254.4214128E-543.9446951
TTTACAC700.040.8057862
TTACACA900.031.7378353
CTTTACA857.2759576E-1231.0197831
TTTCGGT300.00570913429.3226244
ATGTGAC300.00570913429.3226210
GTTTTAT300.00570913429.3226241
GTGTTAG300.005734132629.2964631
CATGTGA406.999523E-427.4654339
CGGCGTG602.8326854E-421.99196629
CGAAACT500.002555533321.99196425
CGGTGGT703.1542335E-521.99196443
ACGAAAC654.863846E-420.30027424
CTTATAC4050.020.0735021
TTATACA3850.018.8334392
TATACAC4050.018.4459193
GGTGTAG600.00730404518.34301435
GTATGCA600.00734185918.32663729
ATTTGCG600.00734185918.32663730
TCGGTGG1102.7105962E-617.99342544
Produced by FastQC (version 0.11.5)