FastQCFastQC Report
Wed 25 May 2016
SRR1780611_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780611_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56070
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2806450.05172106295702No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT53619.561262707330123RNA PCR Primer, Index 14 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG41037.317638665953273No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC29085.186374175138219No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC9631.7174959871589086No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT7041.2555733904048512No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC4580.816836097734974No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3680.656322454075263No Hit
ATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTT2530.4512216871767434RNA PCR Primer, Index 27 (96% over 28bp)
CATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCT1650.294275013376137RNA PCR Primer, Index 14 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATATCGTATGCCGT1590.2835741037988229No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC1360.24255395041911898No Hit
ACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCT1340.23898698056001427RNA PCR Primer, Index 14 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1260.22471910112359553No Hit
TCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTTCTGCTTG1130.20153379703941504RNA PCR Primer, Index 14 (96% over 28bp)
CTTATTACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1060.1890494025325486No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG980.17478152309612985No Hit
CTTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG960.17121455323702514No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCATCTCGTATGCCGTC860.1533797039415017No Hit
TCTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGC830.14802924915284466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACA408.019924E-944.0321431
CTTTACA1050.044.0321431
CTTATTA207.764451E-444.0321431
CTTATAA207.764451E-444.0321431
TCTTTAT207.764451E-444.0321431
TCTTAAT254.369778E-544.0321431
TTATTAC207.798712E-443.9928632
ATTACAC207.798712E-443.9928634
TTGGATG207.798712E-443.9928634
TTTACAC1050.043.9928632
CCGTCTT6900.038.89224244
TTACACA1250.036.9540023
GCCGTCT7550.035.54390343
CTTTATA250.002335621335.194292
TAAACAT250.002335621335.194295
ATAAACA250.002335621335.194294
TATTACA250.002335621335.194293
CTTATAC38450.034.18361
TTATACA38450.034.1531072
ATGCCGT39100.033.30405844
Produced by FastQC (version 0.11.5)