FastQCFastQC Report
Wed 25 May 2016
SRR1780610_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780610_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6059
Sequences flagged as poor quality0
Sequence length76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAACATAAGGGTGTAGATCTCGGTG140.23106123122627495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT1002.0918378E-1035.0000045
ATCTGAC857.991184E-832.94117710
CACATCT1105.838956E-1031.8181847
CATCTGA901.3208592E-731.1111139
ACACATC1309.822543E-1129.6153836
TCTGACG952.1233427E-729.47368611
TGACGCT853.1735417E-628.8235313
CTGACGC904.9543505E-627.22222312
GACGCTG657.0768927E-426.92307714
ACATCTG1055.1036295E-726.6666688
ATACACA1055.1036295E-726.6666684
TATACAC700.001091204925.03
TTATACA700.001091204925.02
CTTATAC700.001091204925.01
Produced by FastQC (version 0.11.5)