FastQCFastQC Report
Wed 25 May 2016
SRR1780610_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780610_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6059
Sequences flagged as poor quality0
Sequence length76
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT224136.986301369863014No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4567.525994388512956RNA PCR Primer, Index 42 (95% over 22bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2844.687242119161578No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC1913.152335368872751No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1552.5581779171480443No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT1071.7659679815151015No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1031.699950486879023No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT1011.6669417395609836No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT540.8912361775870606RNA PCR Primer, Index 42 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC440.7261924409968642RNA PCR Primer, Index 32 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG330.5446443307476482No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG300.4951312097705892No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC280.4621224624525499No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG260.42911371513451063No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT260.42911371513451063No Hit
CTTATACACATCTCCGAGCCCACGCGACATCACTCGATCTCGTATGCCGT240.3961049678164714No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGTCTT210.34659184683941247No Hit
ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT190.3135830995213732RNA PCR Primer, Index 42 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT180.2970787258623535No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTAGTATGCCGT180.2970787258623535No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC170.2805743522033339RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT170.2805743522033339No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT160.26406997854431424No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT160.26406997854431424No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG150.2475656048852946No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT150.2475656048852946No Hit
CTTATACACATCTCCGAGCACACGAGACATCACTCGATCTCGTATGCCGT150.2475656048852946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC140.23106123122627495No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT140.23106123122627495No Hit
CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT140.23106123122627495No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC130.21455685756725532No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT120.1980524839082357No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT120.1980524839082357No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTT110.18154811024921605No Hit
CTTATACACATATCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT110.18154811024921605No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT110.18154811024921605No Hit
ATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGCTT110.18154811024921605RNA PCR Primer, Index 32 (96% over 29bp)
CTTATACACATCTCCGAGCCCAAGAGACATCACTCGATCTCGTATGCCGT100.1650437365901964No Hit
CTTATACACATCTCCGAGCCCACGTGACATCACTCGATCTCGTATGCCGT100.1650437365901964No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT100.1650437365901964No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACACGATCTCGTATGCCGT100.1650437365901964No Hit
CTTATACACATCTCCGCGCCCACGAGACATCACTCGATCTCGTATGCCGT100.1650437365901964No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGTCTT90.14853936293117675No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT90.14853936293117675No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTAGATCTCGTATGCCGT90.14853936293117675No Hit
CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT80.13203498927215712No Hit
ATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGTCTT80.13203498927215712RNA PCR Primer, Index 42 (100% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCCCGTATGCCGT80.13203498927215712No Hit
CTTAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC80.13203498927215712No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT80.13203498927215712No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC80.13203498927215712No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG80.13203498927215712No Hit
CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT80.13203498927215712No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACTCGATCTCGTATGCCGT80.13203498927215712RNA PCR Primer, Index 32 (95% over 23bp)
CTTATACACATCTCCGAGCCAACGAGACATCACTCGATCTCGTATGCCGT80.13203498927215712No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATTTCGTATGCCGT70.11553061561313747No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCG70.11553061561313747No Hit
CTTATACACATCTCCGAGCCCACGAGAAATCACTCGATCTCGTATGCCGT70.11553061561313747No Hit
CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG70.11553061561313747No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT70.11553061561313747No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAC252.5141107E-670.0000133
TCGAACT252.5141107E-670.0000134
AACTCGT252.5141107E-670.0000137
CGAACTC252.5141107E-670.0000135
GAACTCG252.5141107E-670.0000136
ACTCGTA207.309471E-570.038
ACTCGAA308.628194E-870.032
CTATACA150.00211916970.01
ATCTACG150.00211916970.010
ACATCTA150.00211916970.08
TCTACGA150.00211916970.011
ACGAGCC150.00211916970.014
TACGAGC150.00211916970.013
CTACGAG150.00211916970.012
ATCGTAT252.2053851E-456.00000439
CTTATAC4050.055.308641
TTATACA4200.053.3333362
TCGATAT200.006624108652.534
CCTCGTA200.006624108652.538
AAAAACT200.006624108652.567
Produced by FastQC (version 0.11.5)