##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780610_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6059 Sequences flagged as poor quality 0 Sequence length 76 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76068658194421 34.0 34.0 34.0 31.0 34.0 2 33.14160752599439 34.0 34.0 34.0 32.0 34.0 3 33.35731968971778 34.0 34.0 34.0 33.0 34.0 4 33.4612972437696 34.0 34.0 34.0 34.0 34.0 5 33.463937943555045 34.0 34.0 34.0 34.0 34.0 6 37.308961874896845 38.0 37.0 38.0 36.0 38.0 7 36.718105297903946 38.0 37.0 38.0 34.0 38.0 8 36.85492655553722 38.0 37.0 38.0 35.0 38.0 9 36.608681300544646 38.0 37.0 38.0 34.0 38.0 10-11 36.28007922099356 38.0 37.0 38.0 33.0 38.0 12-13 36.00577653078066 38.0 37.0 38.0 33.0 38.0 14-15 36.078808384221816 38.0 36.5 38.0 32.5 38.0 16-17 35.9293612807394 38.0 36.0 38.0 32.5 38.0 18-19 35.84395114705397 38.0 36.0 38.0 31.0 38.0 20-21 35.6109919128569 38.0 36.0 38.0 31.0 38.0 22-23 35.92135665951477 37.5 36.0 38.0 32.5 38.0 24-25 34.52797491335204 37.0 34.0 38.0 28.0 38.0 26-27 34.80425812840403 37.0 34.0 38.0 28.0 38.0 28-29 34.74698795180723 37.0 34.0 38.0 29.0 38.0 30-31 34.0571876547285 37.0 34.0 38.0 25.0 38.0 32-33 34.774880343290974 37.0 35.0 38.0 27.0 38.0 34-35 34.04934807724047 37.0 34.0 38.0 25.0 38.0 36-37 34.13896682620894 37.0 34.0 38.0 25.5 38.0 38-39 28.648209275457997 34.0 22.0 37.0 11.0 38.0 40-41 31.9915827694339 35.0 30.5 37.0 17.0 38.0 42-43 34.60793860372999 37.0 34.5 38.0 28.5 38.0 44-45 35.17321340155141 37.0 35.5 38.0 31.0 38.0 46-47 35.3333883479122 37.5 36.0 38.0 31.0 38.0 48-49 34.86969796996204 37.5 35.0 38.0 28.0 38.0 50-51 34.652582934477635 37.0 34.0 38.0 28.0 38.0 52-53 34.28148209275458 37.0 34.0 38.0 25.0 38.0 54-55 34.75441491995379 37.0 35.0 38.0 27.0 38.0 56-57 34.82142267700941 37.0 35.0 38.0 27.0 38.0 58-59 34.097623370193105 37.0 34.0 38.0 24.0 38.0 60-61 33.97144743356989 37.0 34.0 38.0 24.0 38.0 62-63 34.82480607360951 37.0 35.0 38.0 27.0 38.0 64-65 35.077075424987626 37.0 36.0 38.0 29.0 38.0 66-67 32.97326291467239 37.0 32.0 38.0 21.5 38.0 68-69 30.331077735599933 35.0 24.0 37.5 11.0 38.0 70-71 26.713236507674534 29.0 17.0 35.5 10.5 37.5 72-73 21.442482257798318 22.0 11.0 29.0 10.5 36.0 74-75 20.692358474995874 21.0 11.0 27.5 11.0 35.5 76 20.9511470539693 21.0 11.0 28.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1860287870838633 1101 2 0.23134618830194853 1101 3 0.9229247923785167 1101 4 0.5795592257849904 1101 5 0.5727774057953638 1101 6 -1.9855411366420412 1101 7 0.7108555282459506 1101 8 0.8496480726061577 1101 9 1.0562965243662248 1101 10-11 -0.036042949059790885 1101 12-13 1.659509753809182 1101 14-15 1.864897974086098 1101 16-17 -1.2189936654332953 1101 18-19 -0.533016246479157 1101 20-21 -2.1474365940126816 1101 22-23 0.7318111345455662 1101 24-25 -3.8590998215886216 1101 26-27 -0.907579473556325 1101 28-29 0.605443844624233 1101 30-31 3.115626430571126 1101 32-33 -0.43730171153688957 1101 34-35 1.5488732787295802 1101 36-37 -0.4253749256193373 1101 38-39 1.4921705377277092 1101 40-41 1.9865106471887053 1101 42-43 1.2044205016463323 1101 44-45 0.2940995878363495 1101 46-47 1.482934487162936 1101 48-49 -3.5634789876423554 1101 50-51 -3.5413774214623572 1101 52-53 -3.117933685251799 1101 54-55 -0.7563864383900523 1101 56-57 -1.102745690762525 1101 58-59 -1.025338746695887 1101 60-61 -2.8882645386428862 1101 62-63 -1.025516299281982 1101 64-65 -3.159524082524932 1101 66-67 -6.376264518053048 1101 68-69 -0.44265368964224194 1101 70-71 -3.2980507336208404 1101 72-73 -3.54144797066267 1101 74-75 -1.2691250010196526 1101 76 1.554184938524802 1102 1 -0.8139712129161367 1102 2 0.6210520706548905 1102 3 0.7464542041432196 1102 4 0.5795592257849904 1102 5 0.3963068175600668 1102 6 0.7681353339461907 1102 7 -0.020762118812875485 1102 8 0.2724421902532157 1102 9 0.25482593613092774 1102 10-11 0.9805011685872671 1102 12-13 1.460980342044472 1102 14-15 -0.97517555532567 1102 16-17 1.2276975110372987 1102 18-19 0.08463081234437198 1102 20-21 0.8341810530461444 1102 22-23 0.13806113454556623 1102 24-25 -5.927114527470977 1102 26-27 -0.935153002968093 1102 28-29 -1.0287473318463611 1102 30-31 2.850920548218184 1102 32-33 0.4303453472866394 1102 34-35 2.1701968081413483 1102 36-37 -2.4713308079722793 1102 38-39 1.9498911259630027 1102 40-41 2.4037900589534154 1102 42-43 0.8496410898816222 1102 44-45 2.1065995878363495 1102 46-47 2.150213898927646 1102 48-49 -4.019361340583529 1102 50-51 -0.30424506852118327 1102 52-53 0.8747133735717298 1102 54-55 1.6664076792570057 1102 56-57 0.9781366621786489 1102 58-59 -0.46651521728411893 1102 60-61 -3.7926763033487703 1102 62-63 -2.279192769870214 1102 64-65 -0.22386231781904797 1102 66-67 1.1807207760645966 1102 68-69 -4.343388983759887 1102 70-71 -2.2576095571502535 1102 72-73 -1.376006794192083 1102 74-75 -0.8867720598431816 1102 76 1.1975672914659796 1103 1 0.3765049775600531 1103 2 0.8563461883019485 1103 3 0.39911526856899116 1103 4 0.5319401781659394 1103 5 0.42992026293821795 1103 6 0.5412445776436741 1103 7 0.9400221949126149 1103 8 0.751433786891873 1103 9 -0.4734653803956803 1103 10-11 0.7452070509402091 1103 12-13 0.3321288014282331 1103 14-15 0.2979336883718062 1103 16-17 0.898565858376223 1103 18-19 1.6812694678065583 1103 20-21 -0.626603260679353 1103 22-23 0.06663256311699683 1103 24-25 -4.396302202541001 1103 26-27 -0.828710425937274 1103 28-29 -1.271044250613862 1103 30-31 2.020388335333031 1103 32-33 2.336507812272629 1103 34-35 -0.7933886260323177 1103 36-37 -2.444720163714578 1103 38-39 -0.7980080337008637 1103 40-41 -1.181644114716054 1103 42-43 1.054122882598712 1103 44-45 1.6065995878363495 1103 46-47 0.8028749633534105 1103 48-49 -1.4563361304994942 1103 50-51 -1.2244131357480725 1103 52-53 -1.0286479709660838 1103 54-55 -0.7117435812471911 1103 56-57 1.129397166380329 1103 58-59 -0.477719699076836 1103 60-61 -2.715645491023839 1103 62-63 -1.8142067754724565 1103 64-65 -0.4675597968106473 1103 66-67 -2.2408478513863805 1103 68-69 -0.9218203563089062 1103 70-71 -0.9200745431446506 1103 72-73 -3.371805113519816 1103 74-75 -2.7691250010196526 1103 76 1.586923033762897 1104 1 -0.013971212916139564 1104 2 0.70634618830195 1104 3 0.9229247923785167 1104 4 0.5795592257849904 1104 5 -0.4772225942046333 1104 6 0.2769588633579616 1104 7 0.7233555282459534 1104 8 0.23714807260616055 1104 9 1.2812965243662262 1104 10-11 -0.12979294905979089 1104 12-13 1.484509753809185 1104 14-15 0.7586479740860952 1104 16-17 1.2747563345667103 1104 18-19 1.1919837535208444 1104 20-21 -0.15993659401268445 1104 22-23 -0.26193886545443945 1104 24-25 1.1096501784113784 1104 26-27 1.5236705264436736 1104 28-29 2.5491938446242344 1104 30-31 0.1156264305711261 1104 32-33 0.2376982884631076 1104 34-35 -2.613626721270421 1104 36-37 1.393375074380664 1104 38-39 2.7484205377277107 1104 40-41 0.48026064718870387 1104 42-43 0.5481705016463323 1104 44-45 0.23159958783634949 1104 46-47 1.3016844871629303 1104 48-49 -0.4134789876423568 1104 50-51 2.1398725785376485 1104 52-53 1.5070663147482009 1104 54-55 0.7311135616099449 1104 56-57 1.8722543092374764 1104 58-59 -1.2003387466958841 1104 60-61 0.48673546135711376 1104 62-63 0.061983700718023726 1104 64-65 0.8217259174750708 1104 66-67 -0.48251451805305123 1104 68-69 0.8948463103577566 1104 70-71 1.3894492663791596 1104 72-73 -0.97894797066267 1104 74-75 -3.244125001019654 1104 76 -1.0583150614751986 1105 1 1.1333972081364934 1105 2 0.8037146093545786 1105 3 0.9229247923785167 1105 4 0.42166448894288777 1105 5 0.5727774057953638 1105 6 0.6690641265158561 1105 7 1.1680923703512107 1105 8 -0.2786414010780547 1105 9 0.9049807348925398 1105 10-11 0.48204915620336664 1105 12-13 0.10029922749339448 1105 14-15 1.136279553033468 1105 16-17 1.4273879135140746 1105 18-19 2.1248784903629456 1105 20-21 1.4084844586188936 1105 22-23 0.8222716608613609 1105 24-25 0.8530712310429536 1105 26-27 1.0710389474963051 1105 28-29 2.4820885814663427 1105 30-31 1.9314159042553385 1105 32-33 1.7337509200420556 1105 34-35 0.16400485767695017 1105 36-37 1.8460066533280326 1105 38-39 0.7734205377277092 1105 40-41 1.4749974892939655 1105 42-43 1.2258020805937022 1105 44-45 2.2381785352047743 1105 46-47 2.353000276636621 1105 48-49 2.00231048604185 1105 50-51 2.2267146838008003 1105 52-53 0.9939084200113584 1105 54-55 1.0890082984520575 1105 56-57 1.4827806250269475 1105 58-59 2.027292832251483 1105 60-61 2.4209459876729014 1105 62-63 -1.5748584045451395 1105 64-65 1.720410128001383 1105 66-67 0.9609065345785339 1105 68-69 2.771162099831443 1105 70-71 3.494712424273896 1105 72-73 2.428946766179436 1105 74-75 0.8624539463487686 1105 76 3.860105991156381 1106 1 -2.9250823240272474 1106 2 0.3007906327463914 1106 3 0.25625812571185236 1106 4 0.5795592257849904 1106 5 0.5172218502398067 1106 6 -0.06193002553092697 1106 7 -2.615533360642935 1106 8 -0.24062970517162086 1106 9 -0.8464812534115538 1106 10-11 -0.7547929490597909 1106 12-13 1.5900653093647392 1106 14-15 -1.5274631370250162 1106 16-17 -0.5419103320999668 1106 18-19 0.32809486463195725 1106 20-21 0.623396739320647 1106 22-23 0.5547278012122376 1106 24-25 -2.432016488255286 1106 26-27 -1.0152183624452107 1106 28-29 0.21308273351311868 1106 30-31 0.08784865279334753 1106 32-33 -1.0761906004257824 1106 34-35 1.3613732787295802 1106 36-37 -0.289958258952673 1106 38-39 -1.2543572400500693 1106 40-41 1.4969273138553767 1106 42-43 -0.46571838724255343 1106 44-45 0.884377365614121 1106 46-47 0.1322400427184931 1106 48-49 2.103187679024316 1106 50-51 0.2954281340931999 1106 52-53 0.9792885369704223 1106 54-55 0.23666911716550487 1106 56-57 -0.31107902409585364 1106 58-59 2.78021680885967 1106 60-61 -1.152153427531772 1106 62-63 0.02309481182913231 1106 64-65 -1.7810518603027106 1106 66-67 -1.5741811847197127 1106 68-69 1.744846310357758 1106 70-71 2.028338155268049 1106 72-73 -2.562281303996002 1106 74-75 -3.685791667686317 1106 76 -3.4527595059196408 1107 1 0.6622192632743378 1107 2 -1.1436538116980515 1107 3 0.9229247923785167 1107 4 0.4367020829278445 1107 5 -0.617698784680826 1107 6 0.588863625262718 1107 7 1.0828793377697608 1107 8 0.6561956916537781 1107 9 0.6693917624614656 1107 10-11 1.435683241416399 1107 12-13 1.1892716585710872 1107 14-15 1.4407908312289592 1107 16-17 1.6842801440905149 1107 18-19 1.6098408963779818 1107 20-21 1.5876824536063694 1107 22-23 1.3047278012122376 1107 24-25 1.6989358926970937 1107 26-27 1.4808133835865362 1107 28-29 1.728955749386131 1107 30-31 2.5918169067616006 1107 32-33 2.7174601932250155 1107 34-35 1.8256589930152884 1107 36-37 1.1505179315235239 1107 38-39 2.2734205377277092 1107 40-41 1.2469273138553696 1107 42-43 0.2207895492653833 1107 44-45 1.6065995878363495 1107 46-47 1.4695416300200748 1107 48-49 1.90080672664336 1107 50-51 2.8232059118709714 1107 52-53 3.3284948861767703 1107 54-55 1.240637371133758 1107 56-57 1.4627304997136648 1107 58-59 3.665137443780303 1107 60-61 1.498640223261873 1107 62-63 2.400078938813259 1107 64-65 2.794344965094119 1107 66-67 0.6162950057564771 1107 68-69 2.7210367865482326 1107 70-71 2.6513540282839223 1107 72-73 -0.41942416113885983 1107 74-75 -1.1024583343529848 1107 76 2.0154944623343276 1108 1 -0.5639712129161367 1108 2 0.6563461883019457 1108 3 -0.2770752076214862 1108 4 0.3295592257849904 1108 5 -1.4772225942046369 1108 6 0.12695886335795592 1108 7 -1.2266444717540494 1108 8 0.38714807260615913 1108 9 0.8812965243662276 1108 10-11 0.04520705094020627 1108 12-13 1.734509753809185 1108 14-15 1.083647974086098 1108 16-17 1.0497563345667018 1108 18-19 -0.15801624647915702 1108 20-21 2.0900634059873155 1108 22-23 0.3130611345455634 1108 24-25 0.8096501784113812 1108 26-27 0.748670526443675 1108 28-29 -2.4008061553757685 1108 30-31 0.9406264305711289 1108 32-33 0.7876982884631119 1108 34-35 -0.3136267212704169 1108 36-37 0.8183750743806613 1108 38-39 -1.67657946227229 1108 40-41 0.7802606471887081 1108 42-43 -1.8518294983536663 1108 44-45 1.4315995878363523 1108 46-47 1.9016844871629388 1108 48-49 1.7115210123576432 1108 50-51 1.0148725785376413 1108 52-53 -0.5179336852517977 1108 54-55 -2.618886438390053 1108 56-57 -0.7527456907625307 1108 58-59 0.09966125330411302 1108 60-61 1.9367354613571166 1108 62-63 2.4369837007180237 1108 64-65 1.371725917475068 1108 66-67 1.0174854819469488 1108 68-69 3.169846310357755 1108 70-71 -1.885550733620839 1108 72-73 0.8210520293373307 1108 74-75 -2.0441250010196512 1108 76 -1.1583150614751965 1109 1 -1.702860101805026 1109 2 -0.14365381169805147 1109 3 0.4784803479340738 1109 4 0.5240036702294333 1109 5 0.23944407246202815 1109 6 0.2158477522468445 1109 7 -1.8377555828651637 1109 8 0.5371480726061577 1109 9 -0.29092569785599665 1109 10-11 0.9952070509402091 1109 12-13 1.1733986426980678 1109 14-15 0.08364797408609803 1109 16-17 0.09697855678892608 1109 18-19 0.8280948646319573 1109 20-21 1.2622856282095398 1109 22-23 0.47139446787889483 1109 24-25 0.06798351174470696 1109 26-27 -3.4041072513341035 1109 28-29 -2.425806155375767 1109 30-31 -2.1065957916510953 1109 32-33 0.1738093995742247 1109 34-35 -0.8052933879370912 1109 36-37 1.7378195188251055 1109 38-39 3.7734205377277092 1109 40-41 2.163593980522041 1109 42-43 1.2842816127574466 1109 44-45 2.1621551433919066 1109 46-47 1.5211289316073788 1109 48-49 0.18652101235764462 1109 50-51 0.18431702298208563 1109 52-53 -0.3818225741406849 1109 54-55 0.5977802282766191 1109 56-57 1.577809864793032 1109 58-59 0.11355014219299875 1109 60-61 1.903402128023778 1109 62-63 1.6342059229402395 1109 64-65 0.496725917475068 1109 66-67 0.14804103750250874 1109 68-69 -1.1718203563089062 1109 70-71 1.8061159330458239 1109 72-73 2.6877186960039943 1109 74-75 2.203097221202569 1109 76 0.7694627163025807 1110 1 -1.6776075765525 1110 2 -1.870926538970778 1110 3 0.4683793378330634 1110 4 0.21592286214862355 1110 5 0.5727774057953638 1110 6 0.3724134088125055 1110 7 0.7733555282459506 1110 8 0.9462389816970642 1110 9 1.067660160729858 1110 10-11 -0.14115658542343112 1110 12-13 1.3754188447182756 1110 14-15 1.515466155904285 1110 16-17 1.4883926982030644 1110 18-19 0.5578928444299365 1110 20-21 1.9264270423509586 1110 22-23 -1.0210297745453403 1110 24-25 -0.4471680034068086 1110 26-27 1.6009432537164017 1110 28-29 1.6878302082605927 1110 30-31 0.38835370329839947 1110 32-33 1.471789197554017 1110 34-35 1.8841005514568536 1110 36-37 1.7251932561988426 1110 38-39 3.705238719545889 1110 40-41 0.12571519264325204 1110 42-43 0.5595341380099725 1110 44-45 1.5838723151090761 1110 46-47 1.894866305344749 1110 48-49 1.5728846487212849 1110 50-51 -0.9874001487350839 1110 52-53 1.0525208602027476 1110 54-55 -0.546159165662786 1110 56-57 1.5677088546920217 1110 58-59 0.42920670784956627 1110 60-61 0.8958263704480203 1110 62-63 -0.15619811746379497 1110 64-65 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72-73 -1.8122813039960022 2116 74-75 -1.8524583343529883 2116 76 -4.34164839480853 2117 1 1.1860287870838633 2117 2 0.8563461883019485 2117 3 -1.1604085409548155 2117 4 0.4128925591183261 2117 5 0.23944407246202815 2117 6 0.49362553002462306 2117 7 0.8566888615792863 2117 8 0.3704814059394934 2117 9 -3.568703475633775 2117 10-11 0.03687371760688052 2117 12-13 -2.6738235795241536 2117 14-15 -0.33301869258057337 2117 16-17 -1.333576998766631 2117 18-19 -1.033016246479157 2117 20-21 -1.126603260679353 2117 22-23 0.7213944678788948 2117 24-25 -1.7653498215886216 2117 26-27 -1.251329473556325 2117 28-29 -2.4674728220424313 2117 30-31 -0.2177069027622025 2117 32-33 -5.520635044870225 2117 34-35 0.6530399453962517 2117 36-37 -0.24829159228600162 2117 38-39 -3.559912795605623 2117 40-41 -1.586406019477959 2117 42-43 -2.0351628316870034 2117 44-45 -2.5184004121636505 2117 46-47 -2.3816488461703997 2117 48-49 -2.3134789876423554 2117 50-51 1.1148725785376428 2117 52-53 2.298732981414865 2117 54-55 0.26444689494327633 2117 56-57 0.27225430923747496 2117 58-59 -3.3586720800292227 2117 60-61 -1.9299312053095505 2117 62-63 -2.713016299281982 2117 64-65 -2.878274082524932 2117 66-67 -4.699181184719713 2117 68-69 -2.338487022975574 2117 70-71 -3.2772174002875047 2117 72-73 -0.7706146373293343 2117 74-75 3.4808749989803474 2117 76 -2.84164839480853 2118 1 0.269362120417199 2118 2 0.6063461883019485 2118 3 -0.9937418742881476 2118 4 0.24622589245165472 2118 5 0.5727774057953638 2118 6 -0.5063744699753769 2118 7 1.0233555282459506 2118 8 0.703814739272822 2118 9 -4.568703475633775 2118 10-11 -0.12979294905979089 2118 12-13 -1.8821569128574893 2118 14-15 0.04198130741943373 2118 16-17 -5.916910332099963 2118 18-19 -1.1996829131458213 2118 20-21 -1.7099365940126816 2118 22-23 0.5963944678789019 2118 24-25 -0.09868315492195023 2118 26-27 -3.1679961402229893 2118 28-29 -2.175806155375767 2118 30-31 -2.842706902762206 2118 32-33 -2.603968378203554 2118 34-35 1.2780399453962517 2118 36-37 -1.5816249256193373 2118 38-39 -4.8099127956056265 2118 40-41 0.1635939805220339 2118 42-43 -0.0768294983536677 2118 44-45 -4.1434004121636505 2118 46-47 -2.2566488461703997 2118 48-49 -2.230145654309027 2118 50-51 -5.9267940881290215 2118 52-53 -5.367933685251799 2118 54-55 -4.48555310505672 2118 56-57 0.18892097590414636 2118 58-59 -2.9836720800292227 2118 60-61 -3.679931205309554 2118 62-63 -5.088016299281982 2118 64-65 -1.753274082524932 2118 66-67 -1.3658478513863805 2118 68-69 -1.630153689642242 2118 70-71 -0.3188840669541726 2118 72-73 1.02105202933733 2118 74-75 1.8975416656470117 2118 76 -0.9249817281418657 2119 1 0.9360287870838633 2119 2 0.35634618830194853 2119 3 -1.7020752076214833 2119 4 0.20455922578499042 2119 5 -1.4272225942046362 2119 6 -1.6730411366420412 2119 7 -2.6016444717540494 2119 8 0.03714807260615771 2119 9 0.4312965243662248 2119 10-11 -2.067292949059791 2119 12-13 -3.215490246190818 2119 14-15 0.771147974086098 2119 16-17 -3.7502436654332953 2119 18-19 -4.658016246479157 2119 20-21 -3.8974365940126816 2119 22-23 -0.7369388654544338 2119 24-25 -1.3278498215886216 2119 26-27 -0.31382947355632496 2119 28-29 0.511693844624233 2119 30-31 -2.384373569428874 2119 32-33 -4.62480171153689 2119 34-35 0.11137327872958025 2119 36-37 -0.20662492561933732 2119 38-39 -6.101579462272291 2119 40-41 -5.482239352811295 2119 42-43 -5.201829498353668 2119 44-45 -5.8309004121636505 2119 46-47 -2.173315512837064 2119 48-49 -6.000978987642355 2119 50-51 -2.1976274214623572 2119 52-53 -5.242933685251799 2119 54-55 -2.4438864383900523 2119 56-57 -4.227745690762525 2119 58-59 -7.025338746695887 2119 60-61 -5.700764538642886 2119 62-63 -3.963016299281982 2119 64-65 -0.253274082524932 2119 66-67 0.7799854819469516 2119 68-69 0.5573463103577581 2119 70-71 -2.8605507336208404 2119 72-73 0.20855202933732997 2119 74-75 2.3558749989803474 2119 76 -2.258315061475198 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 3.0 23 3.0 24 10.0 25 21.0 26 43.0 27 91.0 28 173.0 29 250.0 30 332.0 31 492.0 32 753.0 33 1009.0 34 1229.0 35 1114.0 36 374.0 37 161.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 4.538702756230402 14.078230731143753 12.130714639379434 69.25235187324641 2 2.244594817626671 3.581449084007262 81.23452714969467 12.939428948671397 3 2.6572041591021622 15.233536887275129 73.956098366067 8.153160587555702 4 1.3863673873576499 60.68658194421521 22.47895692358475 15.448093744842383 5 1.551411123947846 27.925400231061232 66.61165208780326 3.9115365571876546 6 2.9212741376464764 68.09704571711504 11.619079055949827 17.362601089288663 7 2.838752269351378 27.66133025251692 8.549265555372173 60.95065192275953 8 2.3601254332398085 67.53589701270838 13.76464763162238 16.339329922429442 9 3.300874731803928 15.448093744842383 3.498927215712164 77.75210430764152 10-11 3.078065687407163 38.438686251856744 39.26390493480773 19.219343125928372 12-13 7.889090609011388 6.304670737745502 40.9225944875392 44.88364416570391 14-15 10.851625680805412 8.3182043241459 10.52153820762502 70.30863178742366 16-17 38.09209440501733 33.57814820927546 2.8222478956923585 25.50750949001485 18-19 38.91731308796832 15.654398415580129 2.6819607195906916 42.74632777686087 20-21 10.925895362271001 6.552236342630797 3.1358309952137313 79.38603729988446 22-23 10.884634428123453 42.29245750123783 2.574682290807064 44.24822577983166 24-25 38.14160752599439 42.523518732464105 3.144083182043241 16.190790559498268 26-27 38.52946030698135 46.8064036969797 8.169664961214721 6.494471034824229 28-29 6.114870440666777 45.95642845354019 5.768278593827364 42.16042251196567 30-31 3.102822247895692 13.805908565769927 40.66677669582439 42.424492490509984 32-33 8.120151840237662 36.09506519227595 9.14342300709688 46.64135996038951 34-35 5.52071298894207 12.617593662320514 40.625515761676844 41.23617758706057 36-37 35.5504208615283 33.116025746822906 13.962700115530616 17.37085327611817 38-39 11.825383726687571 14.069978544314242 34.81597623370193 39.28866149529625 40-41 5.6940089123617765 9.481762667106782 44.43802607691038 40.38620234362106 42-43 40.221158607030866 5.264895197227265 43.01039775540518 11.503548440336688 44-45 8.18616933487374 35.28635088298399 41.50024756560489 15.027232216537382 46-47 41.021620729493314 5.94157451724707 16.809704571711503 36.22710018154811 48-49 35.34411619079056 3.449414094735105 11.652087803267866 49.55438191120647 50-51 9.754084832480608 3.251361610826869 45.23023601254332 41.7643175441492 52-53 7.030863178742367 3.5649447103482426 75.2104307641525 14.193761346756892 54-55 4.745007426968146 3.4081531605875557 49.76893876877372 42.077900643670574 56-57 39.84981019970292 3.68047532596138 19.81350057765308 36.65621389668262 58-59 7.963360290476977 15.398580623865325 65.27479782142268 11.363261264235023 60-61 34.50239313418056 47.79666611652088 13.335533916487869 4.365406832810695 62-63 4.901798976728832 87.86928536062057 2.6324475986136324 4.596468064036969 64-65 1.9805248390823569 91.10414259778842 2.434395114705397 4.480937448423832 66-67 2.0382901468889254 88.8100346591847 2.607691038125103 6.5439841558012875 68-69 2.450899488364416 83.7844528800132 3.589701270836772 10.174946360785608 70-71 3.2266050503383394 75.97788413929692 5.644495791384717 15.15101501898003 72-73 4.13434560158442 60.546294768113555 10.12543323980855 25.19392639049348 74-75 4.678989932332068 57.50123782802443 10.802112559828354 27.017659679815154 76 4.10958904109589 59.59729328271992 9.242449249050999 27.05066842713319 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 1.5 24 0.5 25 0.0 26 1.0 27 4.0 28 6.0 29 6.0 30 8.0 31 9.5 32 9.0 33 9.0 34 9.0 35 10.0 36 15.5 37 20.0 38 34.5 39 112.0 40 534.5 41 1157.0 42 1420.0 43 1365.5 44 1103.0 45 685.5 46 476.0 47 357.5 48 184.5 49 102.0 50 74.0 51 61.5 52 36.5 53 27.5 54 31.0 55 24.5 56 21.0 57 24.5 58 25.0 59 29.0 60 29.0 61 21.0 62 17.0 63 18.5 64 19.5 65 16.5 66 9.5 67 5.0 68 4.5 69 2.5 70 1.0 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 6059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.653738240633768 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8201219512195 16.850965505859055 2 9.908536585365853 4.291137151345106 3 4.496951219512195 2.9212741376464764 4 1.676829268292683 1.4523848819937284 5 0.9146341463414633 0.9902624195411784 6 0.6097560975609756 0.7922099356329428 7 0.38109756097560976 0.5776530780656874 8 0.7621951219512195 1.320349892721571 9 0.22865853658536583 0.4456180887935303 >10 2.5152439024390247 9.423997359300214 >50 0.07621951219512195 0.8912361775870606 >100 0.5335365853658537 23.056610001650437 >500 0.0 0.0 >1k 0.07621951219512195 36.986301369863014 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 2241 36.986301369863014 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT 456 7.525994388512956 RNA PCR Primer, Index 42 (95% over 22bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 284 4.687242119161578 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 191 3.152335368872751 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT 155 2.5581779171480443 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGT 107 1.7659679815151015 No Hit CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 103 1.699950486879023 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGT 101 1.6669417395609836 No Hit TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT 54 0.8912361775870606 RNA PCR Primer, Index 42 (95% over 21bp) CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 44 0.7261924409968642 RNA PCR Primer, Index 32 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCG 33 0.5446443307476482 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCG 30 0.4951312097705892 No Hit TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 28 0.4621224624525499 No Hit TCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCG 26 0.42911371513451063 No Hit CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT 26 0.42911371513451063 No Hit CTTATACACATCTCCGAGCCCACGCGACATCACTCGATCTCGTATGCCGT 24 0.3961049678164714 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGTCTT 21 0.34659184683941247 No Hit ACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCT 19 0.3135830995213732 RNA PCR Primer, Index 42 (95% over 24bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGT 18 0.2970787258623535 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTAGTATGCCGT 18 0.2970787258623535 No Hit TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 17 0.2805743522033339 RNA PCR Primer, Index 32 (95% over 21bp) CTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCGT 17 0.2805743522033339 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCCGTCTT 16 0.26406997854431424 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT 16 0.26406997854431424 No Hit CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 15 0.2475656048852946 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGAAATCGTATGCCGT 15 0.2475656048852946 No Hit CTTATACACATCTCCGAGCACACGAGACATCACTCGATCTCGTATGCCGT 15 0.2475656048852946 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCC 14 0.23106123122627495 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACCCGATCTCGTATGCCGT 14 0.23106123122627495 No Hit CTTATACACATCTCCGAGCCCACGAGACATCCCTCGATCTCGTATGCCGT 14 0.23106123122627495 No Hit CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGAACTCGTATGCC 13 0.21455685756725532 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT 12 0.1980524839082357 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT 12 0.1980524839082357 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGACCTCGTATGCCGTCTT 11 0.18154811024921605 No Hit CTTATACACATATCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 11 0.18154811024921605 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCACGTATGCCGT 11 0.18154811024921605 No Hit ATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTTCTGCTT 11 0.18154811024921605 RNA PCR Primer, Index 32 (96% over 29bp) CTTATACACATCTCCGAGCCCAAGAGACATCACTCGATCTCGTATGCCGT 10 0.1650437365901964 No Hit CTTATACACATCTCCGAGCCCACGTGACATCACTCGATCTCGTATGCCGT 10 0.1650437365901964 No Hit ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 10 0.1650437365901964 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACACGATCTCGTATGCCGT 10 0.1650437365901964 No Hit CTTATACACATCTCCGCGCCCACGAGACATCACTCGATCTCGTATGCCGT 10 0.1650437365901964 No Hit ATACACATCTCCGAGCCCACGAGACATCACTCGAGCTCGTATGCCGTCTT 9 0.14853936293117675 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTCTGCCGT 9 0.14853936293117675 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTAGATCTCGTATGCCGT 9 0.14853936293117675 No Hit CTTATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT 8 0.13203498927215712 No Hit ATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGTCTT 8 0.13203498927215712 RNA PCR Primer, Index 42 (100% over 22bp) CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCCCGTATGCCGT 8 0.13203498927215712 No Hit CTTAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC 8 0.13203498927215712 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGACATCGTATGCCGT 8 0.13203498927215712 No Hit TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC 8 0.13203498927215712 No Hit CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 8 0.13203498927215712 No Hit CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT 8 0.13203498927215712 No Hit CTTATACACATCTCCGAGCCCACGAGACACCACTCGATCTCGTATGCCGT 8 0.13203498927215712 RNA PCR Primer, Index 32 (95% over 23bp) CTTATACACATCTCCGAGCCAACGAGACATCACTCGATCTCGTATGCCGT 8 0.13203498927215712 No Hit CTTATACACATCTCCGAGCCCACGAGACATCACTCGATTTCGTATGCCGT 7 0.11553061561313747 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTCACTCGATCTCGTATGCCG 7 0.11553061561313747 No Hit CTTATACACATCTCCGAGCCCACGAGAAATCACTCGATCTCGTATGCCGT 7 0.11553061561313747 No Hit CTTATAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG 7 0.11553061561313747 No Hit CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT 7 0.11553061561313747 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.01650437365901964 0.0 2 0.0 0.0 0.0 0.01650437365901964 0.0 3 0.0 0.0 0.0 0.03300874731803928 0.0 4 0.0 0.0 0.0 0.04951312097705892 0.0 5 0.0 0.0 0.0 0.04951312097705892 0.0 6 0.0 0.0 0.0 0.04951312097705892 0.0 7 0.0 0.0 0.0 0.04951312097705892 0.0 8 0.0 0.0 0.0 0.04951312097705892 0.0 9 0.0 0.0 0.0 0.04951312097705892 0.0 10 0.0 0.0 0.0 0.04951312097705892 0.0 11 0.0 0.0 0.0 0.04951312097705892 0.0 12 0.0 0.0 0.0 0.0825218682950982 0.0 13 0.0 0.0 0.0 0.0825218682950982 0.0 14 0.0 0.0 0.0 0.14853936293117675 0.0 15 0.0 0.0 0.0 0.14853936293117675 0.0 16 0.0 0.0 0.0 0.1650437365901964 0.0 17 0.0 0.0 0.0 0.23106123122627495 0.0 18 0.0 0.0 0.0 0.37960059415745173 0.0 19 0.0 0.0 0.0 0.4621224624525499 0.0 20 0.0 0.0 0.0 0.4951312097705892 0.0 21 0.0 0.0 0.0 0.4951312097705892 0.0 22 0.0 0.0 0.0 0.5446443307476482 0.0 23 0.0 0.0 0.0 0.594157451724707 0.0 24 0.0 0.0 0.0 0.594157451724707 0.0 25 0.0 0.0 0.0 0.6106618253837267 0.0 26 0.0 0.0 0.0 0.6106618253837267 0.0 27 0.0 0.0 0.0 0.643670572701766 0.0 28 0.0 0.0 0.0 0.643670572701766 0.0 29 0.0 0.0 0.0 0.643670572701766 0.0 30 0.0 0.0 0.0 0.6766793200198052 0.0 31 0.0 0.0 0.0 0.6766793200198052 0.0 32 0.0 0.0 0.0 0.6931836936788249 0.0 33 0.0 0.0 0.0 0.8912361775870605 0.0 34 0.0 0.0 0.0 0.9737580458821588 0.0 35 0.0 0.0 0.0 1.0727842878362766 0.0 36 0.0 0.0 0.0 1.320349892721571 0.0 37 0.0 0.0 0.0 1.6669417395609836 0.0 38 0.0 0.0 0.0 1.9475160917643175 0.0 39 0.0 0.0 0.0 2.112559828354514 0.0 40 0.0 0.0 0.0 2.3601254332398085 0.0 41 0.0 0.0 0.0 2.5416735434890247 0.0 42 0.0 0.0 0.0 2.706717280079221 0.0 43 0.0 0.0 0.0 2.8552566430103976 0.0 44 0.0 0.0 0.0 2.9542828849645155 0.0 45 0.0 0.0 0.0 3.1853441161907905 0.0 46 0.0 0.0 0.0 3.3668922264400067 0.0 47 0.0 0.0 0.0 3.6474665786433405 0.0 48 0.0 0.0 0.0 3.796005941574517 0.0 49 0.0 0.0 0.0 4.12609341475491 0.0 50 0.0 0.0 0.0 4.2416240303680475 0.0 51 0.0 0.0 0.0 4.340650272322165 0.0 52 0.0 0.0 0.0 4.390163393299225 0.0 53 0.0 0.0 0.0 4.6212246245254995 0.0 54 0.0 0.0 0.0 4.670737745502558 0.0 55 0.0 0.0 0.0 4.720250866479617 0.0 56 0.0 0.0 0.0 4.835781482092755 0.0 57 0.0 0.0 0.0 4.885294603069814 0.0 58 0.0 0.0 0.0 4.934807724046872 0.0 59 0.0 0.0 0.0 5.05033833966001 0.0 60 0.0 0.0 0.0 5.099851460637069 0.0 61 0.0 0.0 0.0 5.149364581614128 0.0 62 0.0 0.0 0.0 5.264895197227265 0.0 63 0.0 0.0 0.0 5.363921439181383 0.0 64 0.0 0.0 0.0 5.594982670407658 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAC 25 2.5141107E-6 70.00001 33 TCGAACT 25 2.5141107E-6 70.00001 34 AACTCGT 25 2.5141107E-6 70.00001 37 CGAACTC 25 2.5141107E-6 70.00001 35 GAACTCG 25 2.5141107E-6 70.00001 36 ACTCGTA 20 7.309471E-5 70.0 38 ACTCGAA 30 8.628194E-8 70.0 32 CTATACA 15 0.002119169 70.0 1 ATCTACG 15 0.002119169 70.0 10 ACATCTA 15 0.002119169 70.0 8 TCTACGA 15 0.002119169 70.0 11 ACGAGCC 15 0.002119169 70.0 14 TACGAGC 15 0.002119169 70.0 13 CTACGAG 15 0.002119169 70.0 12 ATCGTAT 25 2.2053851E-4 56.000004 39 CTTATAC 405 0.0 55.30864 1 TTATACA 420 0.0 53.333336 2 TCGATAT 20 0.0066241086 52.5 34 CCTCGTA 20 0.0066241086 52.5 38 AAAAACT 20 0.0066241086 52.5 67 >>END_MODULE