FastQCFastQC Report
Wed 25 May 2016
SRR1780609_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780609_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81271
Sequences flagged as poor quality0
Sequence length50
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG880.1082797061682519No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACC207.8224443E-443.98953240
CACATCG207.846222E-443.962467
ATTACCA207.846222E-443.9624633
AATACAC207.846222E-443.962465
TTTACAC800.041.240192
ACGCCTG250.002314459335.2785216
TCTTTAT250.002335588235.213311
CTTTATA250.002342664935.1916282
TTACACA950.034.7072073
CGCTCCC300.005651948529.39876616
CTTATAA300.00570334729.3444231
AGGTCTC300.00572056229.32635337
AAATTAC300.005737817429.30830831
ATGACCC300.005737817429.30830841
CATACAC300.005737817429.3083083
CTTTACA1100.028.0105861
CTATACA406.9599826E-427.5103971
TGACGCG406.9599826E-427.51039710
ATATACA406.9599826E-427.5103971
AACGGCT655.8610567E-727.05382229
Produced by FastQC (version 0.11.5)