##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780609_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 81271 Sequences flagged as poor quality 0 Sequence length 50 %GC 58 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.122245327361544 18.0 10.0 29.0 7.0 31.0 2 19.978602453519706 19.0 10.0 30.0 7.0 31.0 3 20.205103911604386 22.0 10.0 30.0 7.0 33.0 4 22.081431260843353 22.0 10.0 33.0 7.0 37.0 5 21.550122429895044 19.0 10.0 33.0 7.0 37.0 6 21.98303207786295 23.0 10.0 33.0 7.0 37.0 7 21.991792890452928 23.0 10.0 33.0 7.0 37.0 8 21.91268718238978 23.0 10.0 33.0 7.0 37.0 9 22.59089958287704 23.0 10.0 34.0 7.0 39.0 10 22.27074848346889 22.0 10.0 34.0 7.0 39.0 11 22.49903409580293 23.0 10.0 34.0 7.0 39.0 12 22.43794219340232 23.0 10.0 34.0 7.0 39.0 13 22.383100983130515 23.0 10.0 34.0 7.0 39.0 14 22.758745431949897 23.0 10.0 34.0 7.0 40.0 15 22.572615077949084 22.0 10.0 34.0 7.0 40.0 16 22.433524873571137 22.0 10.0 34.0 7.0 40.0 17 22.539909684881447 22.0 10.0 34.0 7.0 40.0 18 22.242448105720367 21.0 10.0 34.0 7.0 40.0 19 22.160032483911852 21.0 9.0 34.0 7.0 40.0 20 22.284308055764047 22.0 10.0 34.0 7.0 40.0 21 22.01675874543195 21.0 8.0 34.0 7.0 40.0 22 22.051789691279794 21.0 9.0 34.0 7.0 40.0 23 21.917153720269223 21.0 9.0 33.0 7.0 40.0 24 21.420875835168758 19.0 8.0 33.0 7.0 40.0 25 21.349263574952936 19.0 8.0 33.0 7.0 39.0 26 21.05519804112168 18.0 8.0 32.0 7.0 39.0 27 20.84157940716861 18.0 8.0 32.0 7.0 39.0 28 20.69169814571003 18.0 8.0 32.0 7.0 39.0 29 20.50384516002018 17.0 8.0 32.0 7.0 39.0 30 20.303958361531173 17.0 8.0 31.0 7.0 39.0 31 20.254002042549004 17.0 8.0 31.0 7.0 39.0 32 19.97772883316312 16.0 8.0 31.0 7.0 38.0 33 19.992875687514612 16.0 8.0 31.0 7.0 38.0 34 19.63530656691809 16.0 8.0 30.0 6.0 38.0 35 19.51533757428849 15.0 8.0 30.0 7.0 38.0 36 19.3867185096775 15.0 8.0 30.0 7.0 38.0 37 19.19218417393658 15.0 8.0 30.0 7.0 38.0 38 19.274587491233035 15.0 8.0 30.0 7.0 38.0 39 18.894291936853243 15.0 8.0 30.0 7.0 38.0 40 18.45810928867616 14.0 8.0 29.0 6.0 37.0 41 18.631713649395234 14.0 8.0 29.0 7.0 37.0 42 18.572811950142118 14.0 8.0 29.0 7.0 37.0 43 18.697407439307995 14.0 8.0 29.0 7.0 37.0 44 18.601936730198965 14.0 8.0 29.0 7.0 37.0 45 18.644362687797614 14.0 8.0 29.0 7.0 37.0 46 18.507130464741422 14.0 8.0 29.0 7.0 37.0 47 18.416212425096283 14.0 8.0 28.0 7.0 36.0 48 18.34034280370612 13.0 8.0 28.0 7.0 36.0 49 18.080606858535024 13.0 7.0 28.0 7.0 36.0 50 18.06079659411106 13.0 7.0 28.0 7.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4493795367181441 1101 2 0.218937489469333 1101 3 0.12431277876406455 1101 4 -0.7105294197555239 1101 5 -0.2485625269926821 1101 6 -1.056523478673519 1101 7 -1.0468064283436789 1101 8 -0.8845517827868115 1101 9 -0.4482503818943435 1101 10 -0.21593496210143925 1101 11 -0.05584673363196302 1101 12 0.14960727843176258 1101 13 0.3727784122579223 1101 14 1.3401055253804515 1101 15 0.32578693425833904 1101 16 -0.11960401712185131 1101 17 -0.24839445764436618 1101 18 0.020780927627225054 1101 19 -1.0097422615745835 1101 20 0.31428907605461376 1101 21 0.026817335719680813 1101 22 -0.31784250892363275 1101 23 -0.6997333353227617 1101 24 -0.6932283093410412 1101 25 0.21392053979266024 1101 26 -0.2069369210437202 1101 27 -1.1894842073414011 1101 28 -0.371966731148202 1101 29 -0.408999168575928 1101 30 -0.2950616540758233 1101 31 -0.8430054953474517 1101 32 -0.3277969208939062 1101 33 -0.5533631278231965 1101 34 0.6352379867028866 1101 35 -0.5839576538825284 1101 36 0.029858116342595054 1101 37 -0.13336230595650278 1101 38 -0.4951664417148258 1101 39 -0.7939394815839762 1101 40 -0.6504975100746613 1101 41 -0.6765178064078619 1101 42 -0.5500678272628363 1101 43 -0.835565776434148 1101 44 -0.8236854812390106 1101 45 -0.33373550345151415 1101 46 -0.1999694885127994 1101 47 -0.13815340264903853 1101 48 -0.41363398512745064 1101 49 -0.6023739251317579 1101 50 -0.8771974956409672 1102 1 -1.1884957182025424 1102 2 -0.6699295443622937 1102 3 0.12155904154927555 1102 4 -0.9076838913002376 1102 5 -2.251316264207471 1102 6 -2.1452987212646555 1102 7 -1.8598145584249792 1102 8 -0.9003529891859721 1102 9 -1.860852007910605 1102 10 -1.7967112537353245 1102 11 -2.3888522411063917 1102 12 -1.7751239043639373 1102 13 -1.1728549473014809 1102 14 -0.8459028669615378 1102 15 -0.22141998942380425 1102 16 -0.8959612161777137 1102 17 -1.4190605997896597 1102 18 -1.7229903810536022 1102 19 -0.8647776667673455 1102 20 -0.3839800034103753 1102 21 -1.1011003144245635 1102 22 -1.0330929678798348 1102 23 -1.1527886723277447 1102 24 -1.2144714249980026 1102 25 -0.7332339317520535 1102 26 -0.7618805349959885 1102 27 -1.0649759461297599 1102 28 -1.2603748087773674 1102 29 -0.9333894124783662 1102 30 -1.7784081004435102 1102 31 -1.7162680182952634 1102 32 -1.7358089848855158 1102 33 -1.5327756638840384 1102 34 -1.8346675994497517 1102 35 -0.978594422830863 1102 36 -1.1105824816117718 1102 37 -2.197747304645201 1102 38 -1.1072828854599095 1102 39 -2.569050942376002 1102 40 -0.7779562040164407 1102 41 -1.7005802244513966 1102 42 -1.2116204105306032 1102 43 -1.5921222936122241 1102 44 -1.1847528822356033 1102 45 -1.5949471478260229 1102 46 -1.95193644366622 1102 47 -1.9303773732758387 1102 48 -2.3158763140023524 1102 49 -1.3082485645233106 1102 50 -1.588776304977447 1103 1 0.016880625883480604 1103 2 -0.7339080389859518 1103 3 -0.48381730253674604 1103 4 -1.266017224633572 1103 5 -0.49002594162682556 1103 6 -0.5813202266409974 1103 7 -0.7431478917583121 1103 8 0.0060986237172535596 1103 9 -0.3858520079106036 1103 10 -0.19052845803639684 1103 11 -1.1401963271278994 1103 12 -1.9170593882349038 1103 13 -0.9857581731079321 1103 14 -0.6166017916927196 1103 15 -1.0362049356603613 1103 16 -0.9712300333820139 1103 17 -1.1859960836606263 1103 18 -1.0318613487955375 1103 19 -1.017465838810356 1103 20 -0.6877434442705912 1103 21 -0.4933046154998344 1103 22 -1.1422327528260716 1103 23 0.09640487605935277 1103 24 -1.4940413174711225 1103 25 -0.5582339317520528 1103 26 -0.2782783844583534 1103 27 -0.6171264837641672 1103 28 -0.49290169049779564 1103 29 0.016610587521633136 1103 30 -0.04668767033598442 1103 31 0.23077499245742672 1103 32 -0.7296261891865896 1103 33 -0.5088509327012432 1103 34 0.6919453037760555 1103 35 0.4310829965239762 1103 36 -0.7181093633322 1103 37 -0.792102143354878 1103 38 -0.030938799438402498 1103 39 -1.0660939531286928 1103 40 -1.072042225521816 1103 41 -0.935257643806235 1103 42 -0.7909214857994193 1103 43 -1.0235739065154483 1103 44 -0.8960432048162481 1103 45 -1.5989794058905389 1103 46 -0.5022052608705216 1103 47 -0.6989257603726138 1103 48 -0.7000160989485913 1103 49 -0.21416254301793458 1103 50 -0.029636520031210978 1104 1 0.15431231987255245 1104 2 0.09778595008508617 1104 3 -0.3974785047225389 1104 4 0.5472341414866477 1104 5 0.7788265173895681 1104 6 -1.3187519206300706 1104 7 0.35330019567338056 1104 8 3.6091879922039993E-4 1104 9 0.1514430740566084 1104 10 0.9727229080838207 1104 11 0.32360148707975256 1104 12 0.073104546191324 1104 13 0.23063526951501956 1104 14 -0.5282138135506429 1104 15 -1.6672158646221114 1104 16 -0.3141262082454013 1104 17 -0.050340345955710575 1104 18 0.30133537251593623 1104 19 0.43308060927707714 1104 20 -0.5415685808826112 1104 21 0.25519265225972987 1104 22 -0.8695551571976559 1104 23 -0.38679184525212307 1104 24 -0.38475169998478265 1104 25 -0.5856929481454962 1104 26 0.2669401947766197 1104 27 0.4134746091320096 1104 28 0.03100541332733897 1104 29 -0.10552056001935028 1104 30 -0.6808406758004644 1104 31 -0.4718206359578758 1104 32 -0.6671944951975171 1104 33 -0.34477989444987855 1104 34 0.5308797300055659 1104 35 0.0794436522616806 1104 36 0.09883052737817977 1104 37 -0.0023480449942212545 1104 38 -0.2008841546296587 1104 39 -0.7438261935658481 1104 40 0.46265722803009623 1104 41 0.7085948152101587 1104 42 -0.853079955744775 1104 43 0.2858523229927492 1104 44 -0.3665350080949352 1104 45 -0.2075859632675865 1104 46 -0.8782981570453856 1104 47 -0.1198273997168755 1104 48 0.3312680540568742 1104 49 0.23802324933179264 1104 50 -0.9162485418891322 1105 1 -0.9669735407831865 1105 2 -1.1177622056526175 1105 3 -1.5140256358700803 1105 4 -0.8061213913002376 1105 5 -2.222838441626827 1105 6 -1.9396535599743316 1105 7 -1.6801270584249792 1105 8 -2.523588876282748 1105 9 -1.399914507910605 1105 10 -1.4353201247030647 1105 11 -1.7157171604612316 1105 12 -0.6811218882349053 1105 13 -0.9810706731079328 1105 14 -1.0061851250260538 1105 15 -0.8247466023270285 1105 16 -1.2785217000486817 1105 17 -1.6766210836606277 1105 18 -1.9177988487955382 1105 19 -0.7138200054770216 1105 20 -1.9742017776039233 1105 21 -0.8584087821664994 1105 22 -0.09483691949273876 1105 23 -0.44109512394064865 1105 24 -0.7726871508044546 1105 25 -0.7097964317520535 1105 26 -1.7386950511250205 1105 27 -0.3207723170975001 1105 28 -1.1830058571644635 1105 29 -0.07401441247836615 1105 30 -0.644083503669318 1105 31 -1.6509958408759076 1105 32 -0.38223035585325604 1105 33 -1.1411425993679103 1105 34 -1.2502421962239438 1105 35 -1.245479503476023 1105 36 -1.7441510299988678 1105 37 -1.3358521433548773 1105 38 -1.1710429661050696 1105 39 -0.8296356197953578 1105 40 -0.3069380588551489 1105 41 -0.9883826438062364 1105 42 -1.0122756524660872 1105 43 -1.0001364065154483 1105 44 -1.0569807048162474 1105 45 -1.2942919058905389 1105 46 -1.693871927537188 1105 47 -0.9286132603726145 1105 48 -1.2057452656152563 1105 49 -0.38864170968460243 1105 50 -0.8239073533645431 1106 1 -0.2997860407831858 1106 2 -1.0774977825756942 1106 3 -0.7402275589470051 1106 4 -0.988453122069469 1106 5 -0.4131028647037489 1106 6 -0.7877304830512557 1106 7 -0.48289148150190186 1106 8 -0.4900552224365953 1106 9 -0.36469816175675973 1106 10 -2.0860412785492173 1106 11 -2.5331450450766155 1106 12 -0.5632132343887513 1106 13 -0.12421971156947009 1106 14 -0.9172428173337472 1106 15 -1.289410063865489 1106 16 -0.01866593081791379 1106 17 -0.7263806990452437 1106 18 -1.229938271872463 1106 19 -1.7956709670154822 1106 20 -1.663384469911616 1106 21 -2.2657405129357286 1106 22 -2.162745573338892 1106 23 -1.8093643547098779 1106 24 -1.473528496958302 1106 25 -1.2178493163674382 1106 26 -1.0507142818942512 1106 27 -1.7350752017128848 1106 28 -1.7422606648567722 1106 29 -1.4718509509399063 1106 30 -1.6607902344385472 1106 31 -1.4890968024143696 1106 32 -0.8828313173917159 1106 33 -0.37744067629098765 1106 34 -1.109977773147019 1106 35 -1.8112246957837144 1106 36 -1.2616991069219452 1106 37 -1.8017175279702613 1106 38 -1.2668362353358376 1106 39 -0.9308375428722826 1106 40 -0.9630678665474584 1106 41 -0.7217961053446977 1106 42 -1.3441266140045478 1106 43 -1.1254969834385236 1106 44 -1.9191201278931693 1106 45 -2.000902482813615 1106 46 -1.3207950044602654 1106 47 -1.2970026834495378 1106 48 -1.4378366117691037 1106 49 -0.3539061327615265 1106 50 -0.9437390841337745 1107 1 -1.2415770855593067 1107 2 -1.6125150041600804 1107 3 -1.2171506358700803 1107 4 -0.4785794136882977 1107 5 -0.35121997147757256 1107 6 -1.0422655002728405 1107 7 -0.14339664797721952 1107 8 -1.1050954061334934 1107 9 -0.6332400676120962 1107 10 -0.9262249754493332 1107 11 0.10669422013578256 1107 12 -0.3752683434587851 1107 13 -0.4350119044512155 1107 14 -1.0980694533842623 1107 15 -0.9532447366553853 1107 16 -0.06700117766062164 1107 17 -0.7990557851531648 1107 18 0.3901535765775961 1107 19 -0.5714459383128414 1107 20 -0.4501812552158633 1107 21 -0.2678071030620224 1107 22 -0.21039195680616984 1107 23 -1.296505571701843 1107 24 -0.34976271050594576 1107 25 -0.12875631981175673 1107 26 -0.0423331108265117 1107 27 -0.8474747424706344 1107 28 -0.46740417805998646 1107 29 -0.9333894124783662 1107 30 0.1953521306590389 1107 31 -0.866364311025162 1107 32 -0.719800318539825 1107 33 -0.09006983817388203 1107 34 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2.4165164618780572 2211 46 3.027202452628103 2211 47 2.2293800247513538 2211 48 3.4769122757070576 2211 49 2.57991459196829 2211 50 3.6638896507676897 2212 1 0.9080879749648467 2212 2 1.0309213573395084 2212 3 0.3419044822401567 2212 4 -0.10256578106401548 2212 5 0.06351736545978781 2212 6 0.9983385660099202 2212 7 0.37640591401596524 2212 8 -0.11949192746385151 2212 9 1.0741873621681357 2212 10 1.3111119619111093 2212 11 0.9270608907198721 2212 12 0.813649273182417 2212 13 0.6512497009078153 2212 14 0.22782078048575727 2212 15 0.25165595672809005 2212 16 -0.25403843233214474 2212 17 -0.24564175295196478 2212 18 -0.010010955094749363 2212 19 -0.23885691492584016 2212 20 0.2249862145220618 2212 21 -0.3152862427964216 2212 22 0.9911005805072612 2212 23 0.5151056634609255 2212 24 1.1450662940774343 2212 25 0.7116479580117243 2212 26 0.03517305911120161 2212 27 0.4227685293591925 2212 28 0.1662190444103402 2212 29 -0.2995311447618292 2212 30 0.2896640357007598 2212 31 0.4236227614862962 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-0.5348613487955376 2213 19 -0.6821325054770213 2213 20 0.16892322239607793 2213 21 -0.46697128216650086 2213 22 0.47110058050726167 2213 23 -0.5025951239406474 2213 24 -0.6953746508044532 2213 25 0.5667660682479472 2213 26 0.740054948874981 2213 27 -0.17445981709749958 2213 28 -0.18256835716446318 2213 29 1.2546105875216327 2213 30 0.857978996330683 2213 31 0.5924416591240913 2213 32 0.43070714414674427 2213 33 0.199482400632089 2213 34 -0.43305469622394455 2213 35 -0.6469170034760232 2213 36 0.8952239700011333 2213 37 0.6988978566451216 2213 38 0.426394533894932 2213 39 -0.3477606197953591 2213 40 6.244411448506071E-4 2213 41 0.2757423561937635 2213 42 -0.358588152466087 2213 43 -1.0645739065154487 2213 44 -0.6280432048162474 2213 45 -0.14797940589053837 2213 46 -0.6038719275371882 2213 47 -0.1579257603726134 2213 48 0.16431723438474322 2213 49 0.9491707903153959 2213 50 -0.11296985336454313 2214 1 0.29865145921681346 2214 2 0.11661279434738248 2214 3 0.3219118641299197 2214 4 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0.3006798561937636 2214 42 1.6205368475339128 2214 43 0.19517609348455167 2214 44 0.4039567951837526 2214 45 -0.13804190589053889 2214 46 0.5951905724628119 2214 47 0.2198242396273855 2214 48 0.2473797343847437 2214 49 -1.1620792096846024 2214 50 -0.11296985336454313 2215 1 1.1383091973120507 2215 2 1.197837992760082 2215 3 1.3780874593680146 2215 4 1.0169192833029363 2215 5 1.8941010425001572 2215 6 2.3401083447875735 2215 7 1.4814552828448626 2215 8 2.1481621157807425 2215 9 2.1748622778036797 2215 10 1.0773286848207455 2215 11 2.048295736364164 2215 12 1.5543691831936677 2215 13 1.2221783348285769 2215 14 1.7988743987834717 2215 15 2.710223635768209 2215 16 1.8501985380465555 2215 17 0.94614677348223 2215 18 1.3090116670774776 2215 19 1.5849151135705988 2215 20 0.9670184604913139 2215 21 2.077727130531912 2215 22 2.0704656598723403 2215 23 1.8753731300276044 2215 24 1.7837364603066561 2215 25 0.3223216238035036 2215 26 -0.3374053685853369 2215 27 1.4717624051247213 2215 28 0.8940030714069636 2215 29 1.4396264605375073 2215 30 1.4842647106163973 2215 31 1.2772035638859975 2215 32 0.4914055568451552 2215 33 0.9836093847590739 2215 34 1.8510722879030403 2215 35 1.2985433139842932 2215 36 2.1072874620646225 2215 37 1.8908343645816323 2215 38 1.0468389783393732 2215 39 1.1954139833792468 2215 40 1.1239895205099302 2215 41 0.712758229209637 2215 42 0.25074518086724495 2215 43 1.4625372045956624 2215 44 1.4039567951837526 2215 45 1.8395126576015244 2215 46 2.1434296597644007 2215 47 1.8641694777226228 2215 48 0.9067299327974432 2215 49 0.9961549172995241 2215 50 1.2441730037783145 2216 1 0.6502139592168135 2216 2 1.9429736814441583 2216 3 1.8393009770331439 2216 4 2.3181225603126663 2216 5 2.821264380953817 2216 6 2.0966367626063125 2216 7 2.688572538349213 2216 8 1.381905075330156 2216 9 2.695599604992619 2216 10 2.5661919720711275 2216 11 1.7966316298613485 2216 12 2.765198676281223 2216 13 2.940048278504971 2216 14 3.275064874973946 2216 15 2.4721283976729715 2216 16 1.4830710418868023 2216 17 2.1454555292425965 2216 18 2.4060418770109138 2216 19 2.3448997525874944 2216 20 1.7853748352993009 2216 21 1.2053512984786607 2216 22 1.8620683224427452 2216 23 2.3472113276722553 2216 24 1.9306898653245774 2216 25 1.2828951005060105 2216 26 1.0284420456491716 2216 27 0.45115308612830773 2216 28 0.19123809444844042 2216 29 1.1875783294571178 2216 30 1.76191448020165 2216 31 1.5659900462208682 2216 32 1.949674886082228 2216 33 1.4914178845030577 2216 34 1.5363001424857323 2216 35 0.9810829965239769 2216 36 1.5184497764527443 2216 37 1.7377365663225426 2216 38 1.0378784048626706 2216 39 1.2051426060110941 2216 40 1.0688179895319472 2216 41 0.7187746142582796 2216 42 0.42547636366294483 2216 43 0.8756196418716478 2216 44 0.29105356937730065 2216 45 1.227633497335269 2216 46 0.7254829111724881 2216 47 0.8438161751112574 2216 48 1.4905752989008718 2216 49 1.0627191774121734 2216 50 0.8789656305064248 >>END_MODULE >>Per sequence quality scores fail #Quality Count 4 5.0 5 4.0 6 102.0 7 1525.0 8 4178.0 9 5168.0 10 4198.0 11 3561.0 12 3586.0 13 3695.0 14 3783.0 15 3647.0 16 3250.0 17 3211.0 18 2869.0 19 2756.0 20 2505.0 21 2237.0 22 2012.0 23 1973.0 24 1903.0 25 1811.0 26 1639.0 27 1667.0 28 1573.0 29 1397.0 30 1370.0 31 1289.0 32 1245.0 33 1183.0 34 1265.0 35 1330.0 36 1704.0 37 1914.0 38 1901.0 39 3815.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.05617700684965 17.79800916572217 9.458926723500714 27.68688710392746 2 25.8903300435628 15.895498510989144 31.740542935197265 26.473628510250798 3 23.65050263931784 19.399293721007492 25.71766066616628 31.232542973508387 4 29.89996431691501 17.814472567090352 22.423742786479803 29.86182032951483 5 22.922075525095053 25.095052355698833 25.25870236615767 26.724169753048443 6 38.09230844950843 24.10576958570708 12.099026713095693 25.702895251688794 7 32.6623272754119 27.426757391935624 12.756087657343947 27.154827675308535 8 27.74175290078872 30.55456435875035 16.565564592536084 25.138118147924843 9 30.570936384889873 18.55543250892088 13.55973914113449 37.31389196505476 10 26.57192075796727 24.597022271440878 24.0802264058078 24.750830564784053 11 35.13227513227513 19.83880890857635 19.102990033222593 25.925925925925924 12 27.66211394118371 21.68081702965424 22.149624707764243 28.507444321397813 13 31.206546483726083 16.565557127914847 21.164092782870856 31.063803605488218 14 36.280855462308956 18.27578569143307 17.23475992419954 28.208598922058425 15 29.922596999864638 23.065847925870322 17.68824680358835 29.32330827067669 16 33.981731444843014 19.125278897476672 18.40168632816834 28.491303329511975 17 39.67539099511487 18.606568471827433 16.08401934364502 25.634021189412675 18 34.61073858593827 16.911547184695788 17.288728922197024 31.18898530716892 19 32.07772832077728 17.827947178279473 20.348199203481993 29.74612529746125 20 36.17210243252578 18.041644051907042 20.07203828884393 25.714215226723248 21 30.23514026993017 19.584741789829504 16.88050531718029 33.299612623060035 22 35.081131657351094 16.47502031369266 15.573831039322384 32.87001698963386 23 40.38816550569818 18.703079233022375 16.644103674896005 24.26465158638344 24 31.132331197634304 26.334401182848694 15.53351404632824 26.999753573188762 25 37.527699807948004 19.65701482247501 15.450337322105678 27.364948047471316 26 34.935680433310765 24.240782913768697 15.867544777497383 24.955991875423155 27 31.841739194662267 24.74117661541492 18.49371560818879 24.923368581734024 28 37.84066569831852 21.803835643418104 16.871414855117063 23.484083803146312 29 32.666904676901105 21.94290216548277 20.381883317534378 25.008309840081743 30 36.93577190713714 20.55668452490917 18.281913910955108 24.225629656998585 31 34.2583472865163 19.78602383531961 20.74509997045208 25.210528907712003 32 31.566312523852318 21.519408093168696 20.5505422940083 26.36373708897069 33 35.888059885252765 19.54519711408239 21.169141365639852 23.39760163502499 34 31.436451164107805 21.92413292128883 21.61509954322158 25.024316371381783 35 33.53647276084949 23.788242536164976 19.06063404124346 23.614650661742072 36 36.97565900444466 19.25364129966388 21.528914935792468 22.24178476009899 37 32.22212647569337 20.529833934484753 22.13878596137038 25.10925362845149 38 33.79541285533135 18.53693968754232 24.107747423886146 23.559900033240176 39 34.550983723621684 19.147521607446258 23.994730491738693 22.306764177193372 40 28.08496997941093 29.090998754792814 22.28304422334825 20.540987042448002 41 31.911750221609374 16.227962178666406 24.018762927213633 27.841524672510587 42 26.44940472526255 19.75056326410007 29.54335594597589 24.256676064661487 43 26.841969198951137 16.071450554591227 26.73486723049082 30.35171301596681 44 27.395591004763485 16.065384094629692 30.394003175658213 26.14502172494861 45 26.554652880354507 16.07582471688823 33.26440177252585 24.105120630231415 46 25.868264946956458 19.77871858616191 26.82083343589239 27.532183030989245 47 30.522221811960765 18.568843924232915 28.79050819087004 22.11842607293628 48 30.20923076923077 22.035692307692308 24.530461538461537 23.224615384615387 49 28.657576055922025 20.46248892389485 25.530422368809685 25.349512651373434 50 28.95404081431701 23.820249612288606 22.077394579425448 25.148314993968935 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 1.0 8 2.0 9 2.5 10 3.0 11 3.0 12 3.0 13 4.0 14 5.0 15 11.5 16 18.0 17 31.0 18 44.0 19 35.0 20 26.0 21 70.0 22 114.0 23 118.5 24 123.0 25 148.0 26 173.0 27 246.0 28 319.0 29 349.0 30 379.0 31 469.5 32 560.0 33 695.0 34 830.0 35 955.5 36 1081.0 37 1240.0 38 1399.0 39 1527.5 40 1656.0 41 1760.5 42 1865.0 43 2118.0 44 2371.0 45 2719.0 46 3067.0 47 3338.5 48 3610.0 49 3979.5 50 4349.0 51 4482.0 52 4615.0 53 4907.5 54 5200.0 55 5166.0 56 5132.0 57 5204.5 58 5277.0 59 5084.5 60 4892.0 61 4805.0 62 4718.0 63 4701.5 64 4685.0 65 4331.5 66 3978.0 67 3791.5 68 3605.0 69 3521.5 70 3438.0 71 3229.5 72 3021.0 73 2777.5 74 2534.0 75 2310.5 76 2087.0 77 1932.0 78 1777.0 79 1617.0 80 1457.0 81 1222.0 82 987.0 83 893.5 84 800.0 85 649.5 86 499.0 87 374.0 88 249.0 89 199.5 90 150.0 91 115.5 92 81.0 93 59.0 94 37.0 95 29.5 96 22.0 97 18.0 98 14.0 99 16.0 100 18.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12181466943928339 2 0.011074060858116672 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0012304512064574078 10 0.0012304512064574078 11 0.0012304512064574078 12 0.0012304512064574078 13 0.0073827072387444475 14 0.00615225603228704 15 0.009843609651659263 16 0.18210677855569637 17 0.004921804825829631 18 0.17595452252340932 19 0.20671580268484452 20 0.24978159491085383 21 0.2620861069754279 22 0.05537030429058335 23 0.020917670509775936 24 0.1365800839167723 25 0.052909401877668544 26 0.044296243432466686 27 0.0467571458453815 28 0.04060488981309446 29 0.05167895067121113 30 0.093514291690763 31 0.05783120670349817 32 0.05167895067121113 33 0.060292109116412994 34 0.061522560322870394 35 0.05660075549704076 36 0.061522560322870394 37 0.0467571458453815 38 0.05413985308412595 39 0.060292109116412994 40 0.1968721930331853 41 0.05783120670349817 42 0.05906165790995558 43 0.04921804825829632 44 0.03445263378080742 45 0.038143987400179644 46 0.020917670509775936 47 0.027069926542062975 48 0.025839475335605565 49 0.018456768096861118 50 0.030761280161435197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 81271.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.4678544622313 #Duplication Level Percentage of deduplicated Percentage of total 1 98.08260416959739 85.79074946782985 2 0.6949328981796697 1.215685791979919 3 0.1702163576512956 0.4466537879440391 4 0.10269251329375687 0.35929175228556315 5 0.06752384435753876 0.2953082895497779 6 0.05345637678305151 0.28054287507228903 7 0.06330360408519257 0.3875921300340835 8 0.05767661705539769 0.4035879957180298 9 0.06893059111498748 0.5426289820477169 >10 0.6302225473370284 9.758708518413703 >50 0.008440480544692345 0.5192504091250262 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTGACGCTGCCGACGAGACCCAATGTGTAGATCTCGGTG 88 0.1082797061682519 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.014765414477488895 0.0 2 0.0 0.0 0.0 0.015995865683946304 0.0 3 0.0 0.0 0.0 0.019687219303318525 0.0 4 0.0 0.0 0.0 0.030761280161435197 0.0 5 0.0 0.0 0.0 0.06521391394224262 0.0 6 0.0 0.0 0.0 0.06521391394224262 0.0 7 0.0 0.0 0.0 0.06644436514870003 0.0 8 0.0 0.0 0.0 0.07136616997452966 0.0 9 0.0 0.0 0.0 0.0922838404843056 0.0 10 0.0 0.0 0.0 0.10458835254887967 0.0 11 0.0 0.0 0.0 0.14519324236197415 0.0 12 0.0 0.0 0.0 0.16611091287175006 0.0 13 0.0 0.0 0.0 0.19071993700089823 0.0 14 0.0 0.0 0.0 0.22886392440107786 0.0 15 0.0 0.0 0.0 0.22886392440107786 0.0 16 0.0 0.0 0.0 0.2510120461173112 0.0 17 0.0 0.0 0.0 0.28054287507228903 0.0 18 0.0 0.0 0.0 0.3703658131436798 0.0 19 0.0 0.0 0.0 0.5770816158285244 0.0 20 0.0 0.0 0.0 0.6570609442482558 0.0 21 0.0 0.0 0.0 0.6767481635515744 0.0 22 0.0 0.0 0.0 0.7997932841973151 0.0 23 0.0 0.0 0.0 0.7997932841973151 0.0 24 0.0 0.0 0.0 0.8157891498812615 0.0 25 0.0 0.0 0.0 0.8403981740104096 0.0 26 0.0 0.0 0.0 0.8539331372814412 0.0 27 0.0 0.0 0.0 0.8846944174428764 0.0 28 0.0 0.0 0.0 0.9142252463978541 0.0 29 0.0 0.0 0.0 0.9511387825915764 0.0 30 0.0 0.0 0.0 1.0040481844692448 0.0 31 0.0 0.0 0.0 1.0532662327275413 0.0 32 0.0 0.0 0.0 1.1147887930504117 0.0 33 0.0 0.0 0.0 1.2538297793800988 0.0 34 0.0 0.0 0.0 1.4519324236197413 0.0 35 0.0 0.0 0.0 1.668491835956245 0.0 36 0.0 0.0 0.0 1.9921005032545434 0.0 37 0.0 0.0 0.0 2.5015073027279104 0.0 38 0.0 0.0 0.0 2.940778383433205 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGACC 20 7.8224443E-4 43.989532 40 CACATCG 20 7.846222E-4 43.96246 7 ATTACCA 20 7.846222E-4 43.96246 33 AATACAC 20 7.846222E-4 43.96246 5 TTTACAC 80 0.0 41.24019 2 ACGCCTG 25 0.0023144593 35.27852 16 TCTTTAT 25 0.0023355882 35.21331 1 CTTTATA 25 0.0023426649 35.191628 2 TTACACA 95 0.0 34.707207 3 CGCTCCC 30 0.0056519485 29.398766 16 CTTATAA 30 0.005703347 29.344423 1 AGGTCTC 30 0.005720562 29.326353 37 AAATTAC 30 0.0057378174 29.308308 31 ATGACCC 30 0.0057378174 29.308308 41 CATACAC 30 0.0057378174 29.308308 3 CTTTACA 110 0.0 28.010586 1 CTATACA 40 6.9599826E-4 27.510397 1 TGACGCG 40 6.9599826E-4 27.510397 10 ATATACA 40 6.9599826E-4 27.510397 1 AACGGCT 65 5.8610567E-7 27.053822 29 >>END_MODULE