FastQCFastQC Report
Wed 25 May 2016
SRR1780609_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780609_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81271
Sequences flagged as poor quality0
Sequence length50
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT1931023.760012796692546No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG30813.7910201670952737No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCTT29103.580613010791057TruSeq Adapter, Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC23162.849724994155357No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGG22782.8029678483099754No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT18112.2283471348943658No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT16752.061005770816158No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGT16662.0499317099580416No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGC11401.402714375361445No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCG6020.7407316262873596No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCT4790.5893861278930984No Hit
CTTTACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC4120.5069458970604521No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGT3980.48971958017004835No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGTCTT3690.4540364951827835No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGG3670.4515755927698687No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCCT3490.4294274710536354No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGG3370.41466205657614646No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCG3330.40974025175031686No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGT3330.40974025175031686No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGGCTT3270.40235754451157235No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCC3070.37774852038242424No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCC3030.3728267155565946No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCC3020.3715962643501372No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCG2940.36175265469847795No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGC2930.3605222034920205No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCGT2930.3605222034920205No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2880.3543699474597335No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCGTCTT2820.34698724022098903No Hit
TATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTCT2750.3383740817757872No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGT2530.3113041552337242No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCC2420.29776919196269275No Hit
CTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC2330.286695131104576No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGC2010.24732069249793898No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGCCTT1970.24239888767210935No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGTCGT1900.2337857292269075No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCCT1600.1968721930331853No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCTTATGCCGT1600.1968721930331853No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCTCTT1550.19071993700089823No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCCCGTATGCCGT1310.16118910804592046No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCG1270.1562673032200908No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG1230.15134549839426117No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCCC1190.14642369356843155No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCC1180.14519324236197415No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCG1170.14396279115551672No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGGATGCCGG1120.1378105351232297No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGCGG1100.13534963271031486No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC1070.13165827909094263No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGG1040.12796692547157043No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGC970.11935376702636856No Hit
ATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCCGTCTT950.11689286461345375No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATTCC930.11443196220053894No Hit
CTTATACACATCTGACGCCTGTCTCTTATACACATCTCCGAGCCCACGAG920.11320151099408152No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTTCCGT910.11197105978762413No Hit
TTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGTC900.1107406085811667No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCCTATGCCC890.1095101573747093No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGGC850.10458835254887969No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTTTGCCCT840.10335790134242226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC207.8149256E-443.99815814
TATGTCC207.8149256E-443.99815843
TAAGACT207.8149256E-443.99815815
ATTACAC207.8149256E-443.9981584
TCTGCCC207.8149256E-443.99815843
AAGACTG207.8149256E-443.99815816
ATTGCGT207.8149256E-443.99815844
TATTACA207.8149256E-443.9981583
TGGCGGC207.8149256E-443.99815844
CTTCCGT207.8149256E-443.99815844
TTTCGTC207.8149256E-443.99815842
CCTATGT254.4073946E-543.99815441
TATTGCG302.4994024E-643.99815443
CTATGTC254.4073946E-543.99815442
GCCCTCT254.4073946E-543.99815443
CTTTACA1050.041.9805031
TTTACAC1050.041.9030042
CCGTCTT4300.039.39369644
TCTTAAT404.0046507E-738.569591
TATCGTA357.209095E-637.71270438
Produced by FastQC (version 0.11.5)