FastQCFastQC Report
Wed 25 May 2016
SRR1780608_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780608_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7857
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATGGGGAGGGGGGTTCATAGTAGAAGAGCGATGGTGAGAGCTAAGGT100.12727504136438844No Hit
ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT90.11454753722794961No Hit
ATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTG90.11454753722794961No Hit
GTGTATGATATGTTTGCGGTTTCGATGATGTGGTCTTTGGAGTAGAAACC80.10182003309151075No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG80.10182003309151075No Hit
CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA80.10182003309151075No Hit
GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT80.10182003309151075No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAGA150.002144820569.9872624
GTAGAAG150.002144820569.9872623
AGAAGAG150.002144820569.9872625
AAGAGCG150.002144820569.9872627
GGTTCAT150.002144820569.9872615
GAAGAGC150.002144820569.9872626
AGAGCGA150.002144820569.9872628
CATAGTA150.002144820569.9872619
ATGTCTT150.002144820569.9872616
CGATGGT150.002144820569.9872632
GATGGTG150.002144820569.9872633
AGGGGGG200.006703702352.4904410
GGTGAGA200.006703702352.4904436
GAGCGAT200.006703702352.4904429
CGGGGCG200.006703702352.4904451
AGCTAAG200.006703702352.4904442
AGAGGGT200.006703702352.4904465
GCGGTGA200.006703702352.4904455
GATGTAG200.006703702352.4904460
CTAAGGT200.006703702352.4904444
Produced by FastQC (version 0.11.5)