##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780608_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7857 Sequences flagged as poor quality 0 Sequence length 76 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.272750413643884 33.0 21.0 34.0 12.0 34.0 2 28.835178821433118 34.0 23.0 34.0 11.0 34.0 3 28.81583301514573 34.0 23.0 34.0 11.0 34.0 4 29.15629375079547 34.0 27.0 34.0 11.0 34.0 5 28.853633702430955 34.0 23.0 34.0 11.0 34.0 6 30.98841797123584 37.0 26.0 38.0 11.0 38.0 7 30.964744813542065 37.0 26.0 38.0 11.0 38.0 8 29.781850579101437 36.0 23.0 38.0 11.0 38.0 9 30.931398752704595 36.0 26.0 38.0 12.0 38.0 10-11 30.877815960290185 37.0 26.0 38.0 11.0 38.0 12-13 31.070955835560646 37.0 26.5 38.0 11.0 38.0 14-15 30.802914598447245 37.0 26.0 38.0 11.0 38.0 16-17 30.656166475754105 37.0 26.0 38.0 11.0 38.0 18-19 30.69950362733868 37.0 25.5 38.0 11.0 38.0 20-21 30.78140511645666 37.0 26.0 38.0 11.0 38.0 22-23 30.58120147639048 37.0 25.5 38.0 11.0 38.0 24-25 30.964872088583427 37.0 26.0 38.0 11.0 38.0 26-27 30.893025327733234 37.0 26.0 38.0 11.0 38.0 28-29 30.899261804760087 37.0 26.0 38.0 11.0 38.0 30-31 30.796614483899706 37.0 26.0 38.0 11.0 38.0 32-33 30.512027491408936 37.0 25.0 38.0 11.0 38.0 34-35 30.732595138093423 37.0 26.0 38.0 11.0 38.0 36-37 30.67544864452081 37.0 26.0 38.0 11.0 38.0 38-39 30.87768868524882 37.0 26.0 38.0 11.0 38.0 40-41 30.52730049637266 37.0 25.0 38.0 11.0 38.0 42-43 30.851533664248443 37.0 26.0 38.0 11.0 38.0 44-45 30.0400280005091 36.0 24.0 38.0 11.0 38.0 46-47 30.706058291968944 37.0 26.0 38.0 11.0 38.0 48-49 30.557082856051927 37.0 25.0 38.0 11.0 38.0 50-51 30.404161893852617 36.5 25.0 38.0 11.0 38.0 52-53 30.521064019345808 36.0 25.5 38.0 11.0 38.0 54-55 30.396907216494846 36.5 25.0 38.0 11.0 38.0 56-57 30.095710831106018 36.5 24.0 38.0 11.0 38.0 58-59 30.059819269441263 36.5 23.5 38.0 11.0 38.0 60-61 30.181366933944254 36.0 24.5 38.0 11.0 38.0 62-63 30.038818887616138 36.0 24.0 38.0 11.0 38.0 64-65 30.10812014763905 36.0 24.0 38.0 11.0 38.0 66-67 30.33155148275423 36.0 24.5 38.0 11.0 38.0 68-69 29.76288659793814 36.0 23.5 38.0 11.0 38.0 70-71 30.0203640066183 36.0 24.0 38.0 11.0 38.0 72-73 29.930889652539136 36.0 24.0 38.0 11.0 38.0 74-75 29.476072292223495 35.5 23.0 38.0 11.0 38.0 76 28.751940944380806 34.0 22.0 37.0 11.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -2.662929544035606 1101 2 -1.5416853005227757 1101 3 -0.2240817466234759 1101 4 -3.753403615122881 1101 5 -0.10577575322712462 1101 6 -1.550792755767425 1101 7 0.3391003563811843 1101 8 -0.6509410606290551 1101 9 -0.6290624236256619 1101 10-11 -2.2480090333231963 1101 12-13 -1.1532474168028308 1101 14-15 -1.829287421477357 1101 16-17 -1.9668924743727523 1101 18-19 -0.5410223179930647 1101 20-21 -1.3350335542897405 1101 22-23 -0.684612140535755 1101 24-25 -1.2675805111178384 1101 26-27 0.47371157358719174 1101 28-29 0.13575270881705492 1101 30-31 -1.5092036388753165 1101 32-33 0.12912324141035114 1101 34-35 -0.7801108684879487 1101 36-37 -1.0491559956418506 1101 38-39 -0.8395489106675349 1101 40-41 0.5089480129599835 1101 42-43 -0.44388996868926966 1101 44-45 -0.32515289848717543 1101 46-47 0.32514274085581363 1101 48-49 -1.9564347122515677 1101 50-51 -1.0801944076586913 1101 52-53 -0.6310292094112526 1101 54-55 -1.6805051153488684 1101 56-57 -2.179806415220906 1101 58-59 -0.5997623029843382 1101 60-61 -0.9532444551295676 1101 62-63 -1.2259397495775488 1101 64-65 -2.132172725976531 1101 66-67 -1.4034523812111033 1101 68-69 0.13853884071798817 1101 70-71 0.07803847681491405 1101 72-73 -2.3448446970962458 1101 74-75 -3.0233045941316696 1101 76 0.16057470614580183 1102 1 -0.21848509959116313 1102 2 -2.5046482634857377 1102 3 -0.0759335984753271 1102 4 -0.8645147262339918 1102 5 -1.031701679153052 1102 6 -0.8841260891007572 1102 7 -0.6979366806558538 1102 8 1.6083181986302044 1102 9 -1.3698031643664024 1102 10-11 -2.859120144434307 1102 12-13 -4.9680622316176475 1102 14-15 -3.977435569625502 1102 16-17 -3.300225807706088 1102 18-19 -4.115096392067137 1102 20-21 -2.7239224431786297 1102 22-23 -2.980908436832049 1102 24-25 -2.2305434740808003 1102 26-27 -6.767029167153549 1102 28-29 -3.475358402294056 1102 30-31 -2.4906851203567975 1102 32-33 -8.83383972155261 1102 34-35 -2.6319627203398 1102 36-37 -1.493600440086297 1102 38-39 -1.5617711328897563 1102 40-41 -0.5836445796326046 1102 42-43 -1.4994455242448268 1102 44-45 -8.12144919478347 1102 46-47 -2.2118942961812245 1102 48-49 -1.5490273048441594 1102 50-51 -6.6357499632142485 1102 52-53 -2.0384366168186645 1102 54-55 -0.6434680783118303 1102 56-57 -3.235361970776463 1102 58-59 -0.9145771177991477 1102 60-61 -0.7125037143888271 1102 62-63 -8.781495305133102 1102 64-65 -5.8729134667172715 1102 66-67 -2.310859788618508 1102 68-69 -5.787387085207939 1102 70-71 -0.921961523185086 1102 72-73 -2.1411409933925434 1102 74-75 -6.7084897793168565 1102 76 -5.839425293854198 1103 1 2.7167000855940238 1103 2 1.5983609957735219 1103 3 2.1462886237468943 1103 4 1.9433556441363784 1103 5 1.4902890615876885 1103 6 1.9723553923807238 1103 7 3.1678040600848867 1103 8 3.8965126430746473 1103 9 1.631354243041006 1103 10-11 2.214375225936063 1103 12-13 0.5128405461601311 1103 14-15 1.1296246155596812 1103 16-17 1.5018575256272477 1103 18-19 2.1991397190439734 1103 20-21 2.366355334599149 1103 22-23 0.8391147113160962 1103 24-25 2.5790630074006806 1103 26-27 1.1461652772908941 1103 28-29 2.0587851162244633 1103 30-31 2.3571158055691264 1103 32-33 -3.3726128697007596 1103 34-35 1.9259076500305703 1103 36-37 2.558482893247035 1103 38-39 1.9197103485917246 1103 40-41 2.5112628277748 1103 42-43 2.1220822535329518 1103 44-45 -2.6289723429316183 1103 46-47 2.432202926040997 1103 48-49 1.2652088062669513 1103 50-51 1.9637870738227896 1103 52-53 2.2729059757739307 1103 54-55 0.6748189587252078 1103 56-57 1.3363972884827966 1103 58-59 2.4991960303489975 1103 60-61 3.3945564707963563 1103 62-63 1.8475556207928214 1103 64-65 2.5587994962456904 1103 66-67 1.6087003965666753 1103 68-69 -0.13634541854127136 1103 70-71 1.5375292175556545 1103 72-73 2.390687710311159 1103 74-75 0.061764850312773234 1103 76 0.9696024839235804 1104 1 -1.4261570572631186 1104 2 -0.7811032899407628 1104 3 0.2891457666040367 1104 4 -1.7798586415779063 1104 5 -0.43381808126945387 1104 6 -0.7597874647621339 1104 7 0.8999469172277443 1104 8 -1.2686659283539257 1104 9 -0.9981100426732787 1104 10-11 -1.4173212026353674 1104 12-13 1.7382869747315617 1104 14-15 -0.6493932415831765 1104 16-17 -2.0383210458013217 1104 18-19 0.33530043332968873 1104 20-21 -0.23185895111513588 1104 22-23 0.3636682827446691 1104 24-25 -0.7133477068850347 1104 26-27 1.0881295630051788 1104 28-29 -2.5639827409183944 1104 30-31 -1.029044908716589 1104 32-33 -0.8391307268436172 1104 34-35 -1.208020921397999 1104 36-37 -0.0174099638958225 1104 38-39 0.4732817771631552 1104 40-41 -0.1940943150823422 1104 42-43 -0.3645248893241906 1104 44-45 -4.341025914360188 1104 46-47 -0.29324350253043363 1104 48-49 -0.6700590508759028 1104 50-51 0.5620013595370743 1104 52-53 0.6791559757739307 1104 54-55 -1.4992881841319345 1104 56-57 0.845325859911366 1104 58-59 -0.15928611250814484 1104 60-61 0.2762528993677833 1104 62-63 0.7337163350785367 1104 64-65 -0.8385219323257367 1104 66-67 -0.9113888891476094 1104 68-69 -1.4957204185412714 1104 70-71 -0.9133636395872031 1104 72-73 0.7054198531683014 1104 74-75 -1.5967172925443691 1104 76 -1.4902189446478467 1105 1 -0.2666332477393105 1105 2 -1.2120556708931431 1105 3 0.3796219570802286 1105 4 -1.8608110225302887 1105 5 -0.6576276050789787 1105 6 0.4640220590473909 1105 7 -0.7905292732484455 1105 8 1.2527626430746466 1105 9 2.4376042430410045 1105 10-11 0.29458355926939817 1105 12-13 -1.6736177871732032 1105 14-15 -1.1755837177736517 1105 16-17 -1.9668924743727523 1105 18-19 -0.8873186142893594 1105 20-21 -2.5461446654008526 1105 22-23 -2.4160936220172395 1105 24-25 -2.3657286592659865 1105 26-27 -1.7559180560424394 1105 28-29 -2.123506550442201 1105 30-31 -1.2814258610975422 1105 32-33 -1.4319878697007624 1105 34-35 -2.2782590166360954 1105 36-37 -2.1936004400862963 1105 38-39 -1.0302896514082747 1105 40-41 -3.1762371722252 1105 42-43 -3.738334413133714 1105 44-45 -1.4029306762649512 1105 46-47 -2.1730054072923366 1105 48-49 -2.1879161937330487 1105 50-51 -1.898712926177211 1105 52-53 -2.3125106908927364 1105 54-55 -0.9564310412747936 1105 56-57 -2.942769378183872 1105 58-59 -2.949762302984336 1105 60-61 -1.010651862536978 1105 62-63 -2.322236045873847 1105 64-65 -1.609950503754309 1105 66-67 0.6909920632333453 1105 68-69 -0.5207204185412699 1105 70-71 -1.1812207824443455 1105 72-73 -2.070770623022174 1105 74-75 -2.4788601496872253 1105 76 -3.3283141827430853 1106 1 1.2710479116809807 1106 2 1.841567517512651 1106 3 1.5375929715729804 1106 4 0.8536817310929017 1106 5 2.491647757239864 1106 6 3.0321380010763725 1106 7 1.955847538345754 1106 8 0.704936556118124 1106 9 2.2984738082583966 1106 10-11 1.7503806607186725 1106 12-13 -0.5598496712311736 1106 14-15 1.4468800503422905 1106 16-17 1.272237960409857 1106 18-19 2.7351451538265827 1106 20-21 2.292985769381758 1106 22-23 2.167239711316096 1106 24-25 3.2291988769658992 1106 26-27 2.1556761468561128 1106 28-29 0.5214209857896819 1106 30-31 0.7178495012212984 1106 32-33 2.778881695516631 1106 34-35 3.3688424326392727 1106 36-37 3.1440807193339886 1106 38-39 2.1371016529395526 1106 40-41 2.432458479948707 1106 42-43 1.6913757317938192 1106 44-45 3.6217070048944677 1106 46-47 1.227719230388825 1106 48-49 2.931649023658256 1106 50-51 2.1186783781706175 1106 52-53 1.4671994540348017 1106 54-55 2.9805254804643404 1106 56-57 3.3065059841349687 1106 58-59 3.2987884216533416 1106 60-61 2.5378988621007004 1106 62-63 3.12269149035804 1106 64-65 3.959614713636995 1106 66-67 2.2656297443927684 1106 68-69 2.574931755371775 1106 70-71 2.340518347990436 1106 72-73 3.171258362485066 1106 74-75 -0.5571210192524454 1106 76 1.6340046578366234 1107 1 -2.299966581072642 1107 2 -1.0453890042264788 1107 3 -0.5203780429197735 1107 4 -1.3941443558636202 1107 5 -3.524294271745646 1107 6 -3.33597794095261 1107 7 0.5094707267515517 1107 8 -2.147237356925352 1107 9 -0.7623957569589948 1107 10-11 -0.10541644073060397 1107 12-13 0.09304887949346252 1107 14-15 -2.1755837177736517 1107 16-17 -3.516892474372753 1107 18-19 -1.9873186142893609 1107 20-21 -2.996144665400852 1107 22-23 -3.3827602886839045 1107 24-25 -3.4990619925993194 1107 26-27 -2.8559180560424373 1107 28-29 -2.3568398837755353 1107 30-31 -1.2480925277642072 1107 32-33 -1.2819878697007567 1107 34-35 -1.678259016636094 1107 36-37 -2.6769337734196306 1107 38-39 -1.3969563180749418 1107 40-41 -1.9262371722251999 1107 42-43 -2.438334413133717 1107 44-45 -2.902930676264951 1107 46-47 -3.10633874062567 1107 48-49 -2.104582860399713 1107 50-51 -1.3987129261772075 1107 52-53 -2.1958440242260693 1107 54-55 -2.0230977079414565 1107 56-57 -2.79276937818387 1107 58-59 -2.699762302984336 1107 60-61 -2.077318529203641 1107 62-63 -0.9389027125405143 1107 64-65 -1.7932838370876425 1107 66-67 -1.9423412700999911 1107 68-69 -2.220720418541273 1107 70-71 -1.0978874491110133 1107 72-73 -1.937437289688841 1107 74-75 -2.5955268163538925 1107 76 -3.3283141827430853 1108 1 -0.17123094888873425 1108 2 -0.6936648662954426 1108 3 0.04284034788482671 1108 4 -0.3562133213808636 1108 5 -0.1875126625502439 1108 6 0.14907953031175936 1108 7 1.3876316462917835 1108 8 -0.22309942589086873 1108 9 1.6927766568341092 1108 10-11 1.8733191914533016 1108 12-13 1.706267270298067 1108 14-15 1.10430133969761 1108 16-17 1.8951764911444862 1108 18-19 1.2201526500784574 1108 20-21 1.9676484380474264 1108 22-23 1.0810328147643702 1108 24-25 1.4750759384351646 1108 26-27 0.5394842428081326 1108 28-29 1.5259187369141145 1108 30-31 0.6421373572932616 1108 32-33 1.3628397165061372 1108 34-35 1.2728904086512607 1108 36-37 1.8914570311780672 1108 38-39 1.230055176177931 1108 40-41 -0.14175441360450947 1108 42-43 -0.34235740163946105 1108 44-45 1.1786785191373497 1108 46-47 -0.6103617291314158 1108 48-49 0.2517389786807449 1108 50-51 0.41852845313313125 1108 52-53 0.1576042516360019 1108 54-55 1.7039137863114142 1108 56-57 0.6445869436552094 1108 58-59 -3.0595324179268637 1108 60-61 0.6450952638998046 1108 62-63 -0.0969486895520042 1108 64-65 0.8831529445215551 1108 66-67 0.8398426379459849 1108 68-69 1.1723830297345934 1108 70-71 1.4911930106590994 1108 72-73 -0.989161427619873 1108 74-75 0.6797605399679476 1108 76 0.8693869666821996 1109 1 -3.340992222098283 1109 2 -0.6684659273034015 1109 3 -1.776788299330029 1109 4 -1.1095289712482383 1109 5 -1.2524993999507714 1109 6 -1.9026446076192762 1109 7 -4.611042093761267 1109 8 -2.9087758184638126 1109 9 -1.8470111415743773 1109 10-11 -1.3733651586793236 1109 12-13 -3.171053684609099 1109 14-15 -0.6768657690557056 1109 16-17 -2.293815551295829 1109 18-19 -2.7924468194175667 1109 20-21 0.24616302690684222 1109 22-23 -1.6404525963762104 1109 24-25 -0.7682927618300894 1109 26-27 -2.322584722709106 1109 28-29 -1.09145526839092 1109 30-31 -1.487836117507797 1109 32-33 -1.5973724850853728 1109 34-35 -2.3962077345848165 1109 36-37 -2.1025747990606583 1109 38-39 -2.157212728331352 1109 40-41 -2.0031602491482765 1109 42-43 -2.933206208005508 1109 44-45 -2.5580588813931584 1109 46-47 -2.7589028431897695 1109 48-49 -2.1494546552715086 1109 50-51 -1.5794821569464403 1109 52-53 0.4964636680816241 1109 54-55 -2.317969502813252 1109 56-57 -2.34148732690182 1109 58-59 -2.1318135850356192 1109 60-61 -1.5080877599728737 1109 62-63 -0.16326168689948517 1109 64-65 -2.640719734523543 1109 66-67 -1.0102899880487115 1109 68-69 -0.8130281108489648 1109 70-71 -1.1619900132135754 1109 72-73 -2.9566680589196075 1109 74-75 -1.4057832266103034 1109 76 -0.5231859776148795 1110 1 -2.0767781752755425 1110 2 -1.5062585694438688 1110 3 -1.679798332774844 1110 4 -2.015883486298403 1110 5 -4.76922180797753 1110 6 -3.01134025979319 1110 7 -3.1745872442629377 1110 8 -2.468976487360134 1110 9 -3.35370010478508 1110 10-11 -2.488749774063937 1110 12-13 -1.2989801060137829 1110 14-15 -2.4879025583533654 1110 16-17 -2.3581968221988383 1110 18-19 -2.2865939766081986 1110 20-21 -3.554840317574765 1110 22-23 -2.180586375640427 1110 24-25 -2.0751489491210613 1110 26-27 -4.018236896622152 1110 28-29 -3.130752927253795 1110 30-31 -2.0430200639960923 1110 32-33 -1.2428574349181503 1110 34-35 -2.4137662630129064 1110 36-37 -1.6385279763181835 1110 38-39 -0.7324635644517521 1110 40-41 -3.284932824399114 1110 42-43 -0.46079818124965755 1110 44-45 -1.2043799516272706 1110 46-47 -2.9679329435242217 1110 48-49 -0.568350976341744 1110 50-51 -1.250886839220687 1110 52-53 -0.9458440242260693 1110 54-55 -2.975996258666097 1110 56-57 -2.432624450647637 1110 58-59 -5.288168100085784 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70-71 0.8687792175556517 2116 72-73 5.162562710311153 2116 74-75 2.321139850312772 2116 76 0.5383524839235818 2117 1 2.800033418927356 2117 2 2.921277662440186 2117 3 2.312955290413562 2117 4 0.7558556441363784 2117 5 3.792372394921024 2117 6 4.097355392380724 2117 7 0.042804060084886686 2117 8 2.636095976407983 2117 9 1.037604243041006 2117 10-11 4.511250225936063 2117 12-13 4.26804887949346 2117 14-15 3.082749615559681 2117 16-17 4.116440858960576 2117 18-19 3.954348052377302 2117 20-21 2.1371886679324774 2117 22-23 5.542239711316096 2117 24-25 3.8342713407340163 2117 26-27 4.885748610624223 2117 28-29 5.251493449557792 2117 30-31 5.726907472235791 2117 32-33 4.601345463632576 2117 34-35 3.571740983363899 2117 36-37 3.2563995599137066 2117 38-39 2.503043681925053 2117 40-41 4.0737628277748 2117 42-43 3.2366655868662804 2117 44-45 4.563735990401717 2117 46-47 5.010327926040997 2117 48-49 3.2287504729336156 2117 50-51 4.359620407156125 2117 52-53 4.429155975773931 2117 54-55 1.1019022920585435 2117 56-57 3.9822306218161323 2117 58-59 5.1085710303489975 2117 60-61 5.097681470796356 2117 62-63 4.22776395412615 2117 64-65 2.798382829579026 2117 66-67 4.707658729900004 2117 68-69 3.9626129147920572 2117 70-71 4.360445884222326 2117 72-73 5.145896043644488 2117 74-75 5.504473183646102 2117 76 3.8550191505902447 2118 1 0.21670008559402376 2118 2 -1.8703890042264781 2118 3 -0.8537113762531057 2118 4 1.3808556441363784 2118 5 -1.4159609384123115 2118 6 -0.9026446076192762 2118 7 0.5428040600848867 2118 8 -1.6972373569253527 2118 9 -0.587395756958994 2118 10-11 -0.3012497740639368 2118 12-13 0.4347155461601311 2118 14-15 -0.4797503844403188 2118 16-17 1.3456075256272477 2118 18-19 -1.0039852809560266 2118 20-21 0.11635533459914882 2118 22-23 -1.0827602886839038 2118 24-25 0.6259380074006806 2118 26-27 1.1149152772908941 2118 28-29 1.1681601162244633 2118 30-31 0.5602408055691264 2118 32-33 0.4555121302992404 2118 34-35 2.8634076500305703 2118 36-37 -3.347767106752965 2118 38-39 -5.705289651408275 2118 40-41 -1.8012371722251999 2118 42-43 2.5283322535329518 2118 44-45 0.8554026570683817 2118 46-47 2.072827926040997 2118 48-49 -1.0004161937330487 2118 50-51 -0.2237129261772104 2118 52-53 -3.8833440242260693 2118 54-55 -0.5439310412747922 2118 56-57 -0.6636027115172034 2118 58-59 1.1710710303489975 2118 60-61 -1.1523185292036437 2118 62-63 1.4569306207928214 2118 64-65 -1.2224505037543096 2118 66-67 -1.1881746034333247 2118 68-69 2.2542795814587286 2118 70-71 0.006279217555654526 2118 72-73 -1.687437289688841 2118 74-75 1.9836398503127732 2118 76 3.4383524839235804 2119 1 3.0917000855940238 2119 2 1.6296109957735219 2119 3 1.3962886237468943 2119 4 2.8808556441363784 2119 5 3.0840390615876885 2119 6 1.5973553923807238 2119 7 -2.0821959399151133 2119 8 2.1777626430746473 2119 9 2.162604243041006 2119 10-11 3.511250225936063 2119 12-13 3.934715546160131 2119 14-15 5.645249615559681 2119 16-17 1.4081075256272477 2119 18-19 1.9960147190439734 2119 20-21 1.4288553345991488 2119 22-23 2.667239711316096 2119 24-25 2.5634380074006806 2119 26-27 3.052415277290894 2119 28-29 3.0431601162244633 2119 30-31 0.9352408055691264 2119 32-33 0.7680121302992404 2119 34-35 3.6759076500305703 2119 36-37 -0.09776710675296485 2119 38-39 3.6072103485917246 2119 40-41 0.5112628277748001 2119 42-43 3.6533322535329518 2119 44-45 0.7304026570683817 2119 46-47 1.197827926040997 2119 48-49 1.9995838062669513 2119 50-51 2.1512870738227896 2119 52-53 3.5541559757739307 2119 54-55 0.9560689587252078 2119 56-57 1.6488972884827966 2119 58-59 1.8585710303489975 2119 60-61 -1.3398185292036437 2119 62-63 -0.7930693792071786 2119 64-65 0.7150494962456904 2119 66-67 -0.3131746034333247 2119 68-69 2.3167795814587286 2119 70-71 3.3812792175556545 2119 72-73 4.812562710311159 2119 74-75 4.733639850312773 2119 76 5.93835248392358 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 9.0 13 44.0 14 123.0 15 185.0 16 273.0 17 300.0 18 293.0 19 290.0 20 207.0 21 177.0 22 124.0 23 86.0 24 90.0 25 66.0 26 94.0 27 109.0 28 97.0 29 150.0 30 154.0 31 229.0 32 260.0 33 360.0 34 555.0 35 813.0 36 1371.0 37 1398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.47125848162847 24.70874407886314 16.47676353859941 29.343233900908977 2 13.949344533536973 27.52959144711722 35.33155148275423 23.189512536591575 3 13.300241822578593 33.206058291968944 26.778668703067325 26.715031182385136 4 10.589283441517118 22.489499809087437 34.83517882143312 32.08603792796232 5 10.436553391879851 35.16609392898053 36.43884434262441 17.95850833651521 6 20.313096601756396 36.83339697085402 23.596792668957615 19.25671375843197 7 20.007636502481862 35.675194094438076 21.000381825124094 23.316787577955964 8 17.780323278605064 35.30609647448135 23.291332569683085 23.622247677230497 9 20.198549064528446 19.46035382461499 22.005854651902762 38.3352424589538 10-11 20.007636502481862 27.192312587501593 27.491408934707906 25.308641975308642 12-13 19.15489372534046 24.03589156166476 26.25684103347334 30.552373679521445 14-15 18.39760722922235 25.887743413516613 24.6722667684867 31.04238258877434 16-17 20.313096601756396 28.840524373170425 24.812269313987528 26.034109711085655 18-19 19.333078783250603 27.033218785796105 24.856815578465064 28.776886852488225 20-21 18.42942598956345 27.695049000890926 24.494081710576555 29.381443298969074 22-23 20.764922998599975 27.128675066819397 24.735904289168893 27.370497645411735 24-25 20.243095329005982 29.655084637902508 23.304060073819524 26.79775995927199 26-27 17.423953162784777 30.864197530864196 25.12409316533028 26.587756141020748 28-29 19.517627593228966 27.96232658775614 28.49688176148657 24.02316405752832 30-31 20.274914089347078 27.618683976072294 26.403207331042385 25.70319460353825 32-33 17.64032073310424 30.196003563701158 26.123202240040726 26.040473463153873 34-35 18.874888634338806 29.355988290696196 25.83046964490264 25.938653430062363 36-37 19.275805014636628 27.707776505027365 26.581392388952523 26.435026091383477 38-39 18.951253659157437 26.97594501718213 28.30596919943999 25.76683212422044 40-41 19.485808832887873 26.632302405498283 26.8995799923635 26.982308769250352 42-43 17.869415807560138 27.033218785796105 27.10958381061474 27.98778159602902 44-45 17.29031436935217 27.446862670230367 27.7523227695049 27.510500190912563 46-47 18.060328369606722 28.1786941580756 27.33867888507064 26.422298587247038 48-49 17.786687030673285 28.967799414534813 26.536846124474987 26.70866743031691 50-51 18.18123965890289 27.30049637266132 26.104110983836073 28.41415298459972 52-53 16.997581774214076 28.248695430826015 26.015018454881 28.738704340078915 54-55 16.475754104620083 28.153239149802722 26.428662339315263 28.94234440626193 56-57 17.570319460353826 27.828687794323532 26.053200967290312 28.547791778032327 58-59 17.882143311696577 28.11505663739341 25.55046455390098 28.452335497009035 60-61 16.946671757668323 27.185948835433372 26.275932289677996 29.591447117220316 62-63 18.327605956471935 26.505027364133895 25.824105892834414 29.343260786559753 64-65 17.793050782741503 27.905052819142167 25.187730686012472 29.114165712103855 66-67 17.385770650375463 29.470535827924145 23.24042255313733 29.903270968563067 68-69 17.15667557591956 29.623265877561412 23.297696321751303 29.922362224767724 70-71 17.576683212422044 30.692376225022272 22.947689957999238 28.78325060455645 72-73 17.72941326205931 30.94692630775105 22.324042255313735 28.999618174875906 74-75 18.276695939926178 30.386916125747742 22.947689957999238 28.388697976326842 76 18.111238386152475 30.20236731576938 22.871324933180603 28.815069364897543 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.5 14 0.5 15 1.5 16 2.5 17 2.0 18 1.5 19 1.5 20 3.5 21 5.0 22 4.0 23 8.0 24 14.0 25 15.0 26 21.5 27 34.0 28 54.0 29 68.0 30 69.0 31 86.5 32 113.0 33 123.0 34 150.0 35 192.0 36 231.0 37 255.0 38 281.5 39 332.5 40 360.5 41 419.0 42 474.0 43 482.5 44 526.0 45 571.5 46 582.0 47 577.0 48 561.0 49 530.0 50 510.0 51 451.5 52 343.5 53 272.5 54 251.0 55 254.5 56 216.0 57 170.0 58 166.0 59 132.5 60 95.0 61 82.0 62 73.0 63 58.5 64 39.5 65 30.0 66 19.0 67 13.0 68 15.0 69 11.5 70 4.5 71 3.0 72 4.5 73 5.5 74 4.0 75 3.0 76 1.5 77 1.0 78 2.5 79 3.0 80 3.0 81 2.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5854651902761868 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 7857.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.17780323278605 #Duplication Level Percentage of deduplicated Percentage of total 1 83.74859347371805 66.31029655084639 2 9.99839254139206 15.833015145729924 3 4.018646519852114 9.545628102329133 4 1.3824144028291272 4.378261422934963 5 0.49831216846166215 1.972763141148021 6 0.176820446873493 0.8400152730049638 7 0.06429834431763383 0.35637011582028766 8 0.06429834431763383 0.407280132366043 9 0.032149172158816915 0.22909507445589922 >10 0.016074586079408457 0.12727504136438844 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTATGGGGAGGGGGGTTCATAGTAGAAGAGCGATGGTGAGAGCTAAGGT 10 0.12727504136438844 No Hit ATAATAAAGGTGGATGCGACAATGGATTTTACATAATGGGGGTATGAGTT 9 0.11454753722794961 No Hit ATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAATCGTGTG 9 0.11454753722794961 No Hit GTGTATGATATGTTTGCGGTTTCGATGATGTGGTCTTTGGAGTAGAAACC 8 0.10182003309151075 No Hit GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG 8 0.10182003309151075 No Hit CTATAGTTGTAGCAGGAATCTTCTTACTCATCCGCTTCCACCCCCTAGCA 8 0.10182003309151075 No Hit GTATTATACCATAGCCGCCTAGTTTTAAGAGTACTGCGGCAAGTACTATT 8 0.10182003309151075 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012727504136438845 0.0 2 0.0 0.0 0.0 0.012727504136438845 0.0 3 0.0 0.0 0.0 0.012727504136438845 0.0 4 0.0 0.0 0.0 0.02545500827287769 0.0 5 0.0 0.0 0.0 0.02545500827287769 0.0 6 0.0 0.0 0.0 0.02545500827287769 0.0 7 0.0 0.0 0.0 0.02545500827287769 0.0 8 0.0 0.0 0.0 0.02545500827287769 0.0 9 0.0 0.0 0.0 0.038182512409316534 0.0 10 0.0 0.0 0.0 0.038182512409316534 0.0 11 0.0 0.0 0.0 0.05091001654575538 0.0 12 0.0 0.0 0.0 0.05091001654575538 0.0 13 0.0 0.0 0.0 0.05091001654575538 0.0 14 0.0 0.0 0.0 0.07636502481863307 0.0 15 0.0 0.0 0.0 0.08909252895507191 0.0 16 0.0 0.0 0.0 0.10182003309151076 0.0 17 0.0 0.0 0.0 0.10182003309151076 0.0 18 0.0 0.0 0.0 0.1145475372279496 0.0 19 0.0 0.0 0.0 0.12727504136438844 0.0 20 0.0 0.0 0.0 0.12727504136438844 0.0 21 0.0 0.0 0.0 0.14000254550082727 0.0 22 0.0 0.0 0.0 0.14000254550082727 0.0 23 0.0 0.0 0.0 0.14000254550082727 0.0 24 0.0 0.0 0.0 0.14000254550082727 0.0 25 0.0 0.0 0.0 0.14000254550082727 0.0 26 0.0 0.0 0.0 0.14000254550082727 0.0 27 0.0 0.0 0.0 0.20364006618302152 0.0 28 0.0 0.0 0.0 0.24182257859233805 0.0 29 0.0 0.0 0.0 0.24182257859233805 0.0 30 0.0 0.0 0.0 0.2545500827287769 0.0 31 0.0 0.0 0.0 0.31818760341097113 0.0 32 0.0 0.0 0.0 0.31818760341097113 0.0 33 0.0 0.0 0.0 0.36909761995672646 0.0 34 0.0 0.0 0.0 0.42000763650248185 0.0 35 0.0 0.0 0.0 0.5091001654575538 0.0 36 0.0 0.0 0.0 0.6363752068219423 0.0 37 0.0 0.0 0.0 0.7381952399134529 0.0 38 0.0 0.0 0.0 1.0309278350515463 0.0 39 0.0 0.0 0.0 1.5400280005091003 0.0 40 0.0 0.0 0.0 2.125493190785287 0.0 41 0.0 0.0 0.0 2.596410843833524 0.0 42 0.0 0.0 0.0 2.927325951380934 0.0 43 0.0 0.0 0.0 3.449153620974927 0.0 44 0.012727504136438845 0.0 0.0 3.9709812905689192 0.0 45 0.012727504136438845 0.0 0.0 4.543718976708668 0.0 46 0.012727504136438845 0.0 0.0 5.2691867124856815 0.0 47 0.012727504136438845 0.0 0.0 5.714649357261041 0.0 48 0.012727504136438845 0.0 0.0 6.300114547537228 0.0 49 0.012727504136438845 0.0 0.0 6.669212167493955 0.0 50 0.012727504136438845 0.0 0.0 7.165584828815069 0.0 51 0.012727504136438845 0.0 0.0 7.611047473590429 0.0 52 0.012727504136438845 0.0 0.0 8.094692630775105 0.0 53 0.012727504136438845 0.0 0.0 8.667430316914853 0.0 54 0.012727504136438845 0.0 0.0 9.074710449280897 0.0 55 0.012727504136438845 0.0 0.0 9.672903143693521 0.0 56 0.012727504136438845 0.0 0.0 10.34746086292478 0.0 57 0.012727504136438845 0.0 0.0 10.79292350770014 0.0 58 0.012727504136438845 0.0 0.0 11.352933689703448 0.0 59 0.012727504136438845 0.0 0.0 11.912943871706759 0.0 60 0.012727504136438845 0.0 0.0 12.269313987527045 0.0 61 0.012727504136438845 0.0 0.0 12.600229095074456 0.0 62 0.012727504136438845 0.0 0.0 12.918416698485427 0.0 63 0.012727504136438845 0.0 0.0 13.376606847397225 0.0 64 0.012727504136438845 0.0 0.0 13.783886979763269 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAGA 15 0.0021448205 69.98726 24 GTAGAAG 15 0.0021448205 69.98726 23 AGAAGAG 15 0.0021448205 69.98726 25 AAGAGCG 15 0.0021448205 69.98726 27 GGTTCAT 15 0.0021448205 69.98726 15 GAAGAGC 15 0.0021448205 69.98726 26 AGAGCGA 15 0.0021448205 69.98726 28 CATAGTA 15 0.0021448205 69.98726 19 ATGTCTT 15 0.0021448205 69.98726 16 CGATGGT 15 0.0021448205 69.98726 32 GATGGTG 15 0.0021448205 69.98726 33 AGGGGGG 20 0.0067037023 52.49044 10 GGTGAGA 20 0.0067037023 52.49044 36 GAGCGAT 20 0.0067037023 52.49044 29 CGGGGCG 20 0.0067037023 52.49044 51 AGCTAAG 20 0.0067037023 52.49044 42 AGAGGGT 20 0.0067037023 52.49044 65 GCGGTGA 20 0.0067037023 52.49044 55 GATGTAG 20 0.0067037023 52.49044 60 CTAAGGT 20 0.0067037023 52.49044 44 >>END_MODULE